蒺藜皂苷对环磷酰胺致小鼠生精细胞凋亡的保护作用
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摘要
目的研究蒺藜皂苷对环磷酰胺(cyclophosphamide,CP)所致小鼠生精细胞凋亡的保护作用,探讨蒺藜皂苷对生殖功能障碍小鼠的保护机制。方法将8周龄的雄性昆明种小鼠随机分为3组,对照组采取腹腔注射生理盐水,模型组和治疗组采取连续5d腹腔注射CP 80mg/(kg·d),腹腔注射同时将对照组和模型组小鼠连续灌胃生理盐水,治疗组小鼠连续灌胃蒺藜皂苷200mg/(kg·d)35d。比较各组小鼠体质比及睾丸重量;检测附睾精子密度、活精子百分率、精子活力和精子畸形率;HE染色观察睾丸生精上皮的发育;TUNEL法检测生精细胞的凋亡;Western blot检测睾丸组织中凋亡相关蛋白Caspase-8表达水平的变化。结果与模型组比较,蒺藜皂苷显著提高了生精功能损伤小鼠的精子密度、活精子百分比、精子活力,降低了精子畸形率,促进了生精小管上皮精原细胞及精子细胞的发育,上调了睾丸组织中Caspase-8的表达水平,使生精细胞的凋亡减少。结论蒺藜皂苷能显著修复CP所致的小鼠睾丸损伤,其修复机制可能通过上调Caspase-8的表达,拮抗环磷酰胺引起的生精功能损伤,减少生精细胞的凋亡。
引文
[1] 余华,周艳艳,黎砚书.蒺藜皂苷的主要药理作用研究进展[J].江西化工,2015(2):142-145.
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[4] Tummers B,Green DR.Caspase-8:regulating life and death [J].Immunol Rev,2017,277(1):76-89.
[5] 王正引,郭明章,陈燕.四物汤对血虚证小鼠肝细胞凋亡相关分子Caspase3及Caspase8表达的影响[J].陕西中医药大学学报,2017,40(6):98-102.
[6] Potnuri AG,Allakonda L,Lahkar M.Crocin attenuates cyclo phosphamide inducedtesticular toxicity by preserving glutathione redox system[J].Biomed Pharmacother,2018,101:174-180.
[2] Moallem SA,Hales BF.Induction of apoptosis and cathepsin D in limbs exposed in vitro to an activated analog of cyclophospamide[J].Teratology,1995,52(1):3-14.
[3] Pavin NF,Lzaguirry AP,Soares MB,et al.Tribulus terrestris Protects against Male Reproductive Damage Induced by Cyclophosphamide in Mice [J].Oxidative Medicine and Cellular Longevity,doi:10.1155/2018/5758191.
[4] Tummers B,Green DR.Caspase-8:regulating life and death [J].Immunol Rev,2017,277(1):76-89.
[5] 王正引,郭明章,陈燕.四物汤对血虚证小鼠肝细胞凋亡相关分子Caspase3及Caspase8表达的影响[J].陕西中医药大学学报,2017,40(6):98-102.
[6] Potnuri AG,Allakonda L,Lahkar M.Crocin attenuates cyclo phosphamide inducedtesticular toxicity by preserving glutathione redox system[J].Biomed Pharmacother,2018,101:174-180.