氮掺杂碳量子点荧光猝灭法测定柠檬黄
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摘要
以甘氨酸和乙二胺为前驱物,采用水热法一步合成了氮掺杂碳量子点(N-CQDs)。该量子点近乎球形,分散性好,稳定性高。在pH=5的B-R缓冲溶液中,柠檬黄对N-CQDs的荧光强度有明显猝灭作用,且其猝灭程度与柠檬黄浓度具有良好线性关系,基于此构建了荧光传感体系,建立了柠檬黄检测新方法。方法线性范围为0.16～20.0μmol·L~(-1),检出限为0.076μmol·L~(-1),应用于饮料和枸杞酒中柠檬黄的测定,加标回收率为92.3%～103.5%。光谱分析和荧光寿命测量结果表明柠檬黄对量子点的荧光猝灭为内滤效应和荧光共振能量转移协同作用的结果。
Nitrogen doped carbon quantum dots(N-CQDs)were synthesized through one-step hydrothermal method using glycine and ethylenediamine as precursors.The N-CQDs are near spherical with good dispersion and high stability.Based on the obvious fluorescence quenching effect of tartrazine on quantum dots in the pH=5 B-R buffer solution,a fluorescence sensing system was constructed and a new method for detection of tartrazine was established.Under the optimum experimental conditions,the linear range of this method is 0.16～20.0 μmol·L~(-1) and the detection limit is 0.076 μmol·L~(-1).The proposed method was applied to the analysis of tartrazine in drink and wine and the recovery rate of the actual sample is range of 92.3%～103.5%,indicating the method could be used for accurate,sensitive and rapid analysis of actual samples.Meanwhile,the results of spectroscopic analysis and fluorescence lifetime measurement showed fluorescence quenching results from the synergistic effect of internal filtration and fluorescence resonance energy transfer.
Nitrogen doped carbon quantum dots(N-CQDs)were synthesized through one-step hydrothermal method using glycine and ethylenediamine as precursors.The N-CQDs are near spherical with good dispersion and high stability.Based on the obvious fluorescence quenching effect of tartrazine on quantum dots in the pH=5 B-R buffer solution,a fluorescence sensing system was constructed and a new method for detection of tartrazine was established.Under the optimum experimental conditions,the linear range of this method is 0.16～20.0 μmol·L~(-1) and the detection limit is 0.076 μmol·L~(-1).The proposed method was applied to the analysis of tartrazine in drink and wine and the recovery rate of the actual sample is range of 92.3%～103.5%,indicating the method could be used for accurate,sensitive and rapid analysis of actual samples.Meanwhile,the results of spectroscopic analysis and fluorescence lifetime measurement showed fluorescence quenching results from the synergistic effect of internal filtration and fluorescence resonance energy transfer.
引文
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[3]Karim-Nezhad G,Khorablou Z,Zamani M,et al.Voltammetric sensor for tartrazine determination in soft drinks using poly(p-aminobenzenesulfonic acid)/zinc oxide nanoparticles in carbon paste electrode[J].J Food Drug Anal,2017,25(2):293-301.
[4]Al-Degs Y S.Determination of three dyes in commercial soft drinks using HLA/GO and liquid chromatography[J].Food Chem,2009,117(3):485-490.
[5]Gan T,Sun J Y,Meng W,et al.Electrochemical sensor based on graphene andmesoporous TiO2 for the simultaneous determination of trace colourants in food[J].Food Chem,2013,141(4):3731-3737.
[6]Soponar F,Mot A C,Sarbu C.Quantitative determination of some food dyes using digital processing of images obtained by thin-layer chromatography[J].J Chromatogr A,2008,1188(2):295-300.
[7]孙延春,敬铭.分光光度法测定饮料中色素含量[J].四川理工学院学报:自然科学版,2012,25(5):25-28.
[8]Qu S,Zhu X S,Feng Y L,et al.Aqueous two-phase based on ionic liquid liquid–liquid microextraction for simultaneous determination of five synthetic food colourants in different food samples by high-performance liquid chromatography[J].Food Chem,2015,174:380-386.
[9]张秋菊,曹林波,王硕,等.绿色高效液相色谱法测定饮料中4种合成着色剂[J].中国卫生检验杂志,2018,28(7):785-788.
[10]Xu H,Yang X P,Li G,et al.Green synthesis of fluorescent carbon dots for selective detection of tartrazine in food samples[J].J Agric Food Chem,2015,63(30):6707-6714.
[11]黄颖,程正军.一步水热法合成硫、氮掺杂碳量子点高效检测柠檬黄和pH[J].分析试验室,2018,37(5):589-593.
[12]Tuerhong M,Xu Y,Yin X B.Review on carbon dots and their applications[J].Chinese J Anal Chem,2017,45(1):139-150.
[13]Sheng M L,Gao Y,Sun J Y,et al.Carbon nanodots-chitosan composite film:a platform for protein immobilization,direct electrochemistry and bioelectrocatalysis[J].Biosens Bioelectron,2014,58(8):351-358.
[14]Liu Y L,Lu Q J,Hu X J,et al.A nanosensor based on carbon dots for recovered fluorescence detection clenbuterol in pork samples[J].J Fluoresc,2017,27:1847-1853.
[15]Hou J,Li H Y,Wang L,et al.Rapid microwave-assisted synthesis of molecularly imprinted polymers on carbon quantum dots for fluorescent sensing of tetracycline in milk[J].Talanta,2016,146:34-40.
[16]Feng J,Ju Y Y,Liu J J,et al.Polyethyleneimine-templated copper nanoclusters via ascorbic acid reduction approach as ferric ion sensor[J].Anal Chim Acta,2015,854:153-160.
[17]胡月芳,张亮亮,林丽云,等.基于枸杞为原料的碳量子点制备及作为荧光探针高灵敏检测D-青霉胺[J].中国科学:化学,2017,47(2):258-266.
[18]Huang S,He Q,Xu S,et al.Polyaniline-based photothermal paper sensor for sensitive and selective detection of 2,4,6-trinitrotoluene[J].Anal Chem,2015,87(10):5451-5456.