高效液相色谱法测定广西不同产地楠藤中5种成分的含量
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摘要
目的:采用高效液相色谱法同时测定广西区内不同产地楠藤中绿原酸、隐绿原酸、3,5-O-二咖啡酰基奎宁酸、3,4-O-二咖啡酰基奎宁酸及4,5-O-二咖啡酰基奎宁酸5种成分的含量。方法:色谱柱为Kromasil C18(4.6mm×250mm,5μm);以乙腈(A)-0.4%磷酸水溶液(B)为流动相,梯度洗脱:0～15 min,89%B;15～25 min,89%～80%B;25～50 min,80%B;流速1.0mL·min-1;检测波长328nm;柱温30℃。结果:5种成分在各自进行范围内均呈良好的线性关系,平均加样回收率在98.92%～102.71%之间;广西区内不同产地的楠藤样品中5种成分的总含量在0.418%～1.580%,不同地区的总含量有一定的差异。结论:该高效液相色谱法快速、准确,可用于楠藤中5种咖啡酰基奎宁酸类成分的含量测定;楠藤中该5种成分含量具在一定的地域差异性。
Objective:To develop an HPLC method for the simultaneous determination of five components,including chlorogenic acid,cyrptochlorogenic acid,3,4-O-dicaffeoylquinic acid,3,5-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid,in Mussaenda erosa Champ.from different districts in Guangxi.Methods:The separation was completed on a Kromasil C18(4.6 mm×250 mm,5μm)column.The mobile phase was acetonitrile-0.4%phosphoric acid with the gradient elution:0-15 min,89% B;15-25 min,89%-80% B;25-50 min,80% B.The flow rate was 1.0 mLmin-1,the detection wavelength was 328 nm and the column temperature was 30℃.Results:The standard curve revealed a good linear relationship for this five components and the average recovers were between 98.92%to 102.71%.The total content of the five components in the Nantong samples from different regions in Guangxi was 0.418%to 1.580%,and the total content in different regions had a certain difference.Conclusion:The developed method can be used for the rapid and accurate determination of five components in Mussaenda erosa Champ.
Objective:To develop an HPLC method for the simultaneous determination of five components,including chlorogenic acid,cyrptochlorogenic acid,3,4-O-dicaffeoylquinic acid,3,5-O-dicaffeoylquinic acid and 4,5-O-dicaffeoylquinic acid,in Mussaenda erosa Champ.from different districts in Guangxi.Methods:The separation was completed on a Kromasil C18(4.6 mm×250 mm,5μm)column.The mobile phase was acetonitrile-0.4%phosphoric acid with the gradient elution:0-15 min,89% B;15-25 min,89%-80% B;25-50 min,80% B.The flow rate was 1.0 mLmin-1,the detection wavelength was 328 nm and the column temperature was 30℃.Results:The standard curve revealed a good linear relationship for this five components and the average recovers were between 98.92%to 102.71%.The total content of the five components in the Nantong samples from different regions in Guangxi was 0.418%to 1.580%,and the total content in different regions had a certain difference.Conclusion:The developed method can be used for the rapid and accurate determination of five components in Mussaenda erosa Champ.
引文
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[2]广东省植物研究所.海南植物志[M].北京:科学出版社,1973:313.
[3]李祖晟,朱志安.二咖啡酰基奎宁酸药理实验研究进展[J].医学综述,2004,10(4):249-251.
[4]赵昱,赵军,李湘萍,等.咖啡酰奎尼酸类化合物研究进展[J].中国中药杂志,2006,31(11):869-874.
[5]朱乃亮,彭平,赵丽敏,等.植物中常见咖啡酰基奎宁酸类化合物研究进展[J].中华中医药学会中药化学分会第八届学术年会论文集,2013:200-209.
[6]周彩霞.关于中药材质量和检验方法问题的探讨[J].临床医药文献杂志,2016,32(3):6506-6507.
[7]金璟研.浅谈影响中药材质量的几种因素[J].中国现代药物应用,2014,17(8):211-212.
[8]傅博,李京玉.浅谈影响中药材质量的因素[J].中国农村卫生,2016,6(84):43.
[9]韩亚平,雷振宏,赵丹.中药质量的影响因素及对策[J].现代农业科技,2015(24):113-114.
[10]李恩泽,刘月芬,刘玉君,等.不同产地黄芩中6种化学成分含量测定[J].中国医院药学杂志,2018,38(9):946-948.