蛹虫草的分子生物学研究进展
详细信息    查看全文 | 推荐本文 |
  • 英文篇名:Recent Advances in Molecular Biological Research on Cordyceps militaris(L.) Link
  • 作者:乔宇琛 ; 刘桂君 ; 王平 ; 周思静 ; 郑洁 ; 尚宏忠
  • 英文作者:QIAO Yuchen;LIU Guijun;WANG Ping;ZHOU Sijing;ZHENG Jie;SHANG Hongzhong;Beijing Radiation Center;
  • 关键词:蛹虫草 ; 遗传多样性 ; 分子遗传学 ; 基因组学 ; 转录组学 ; 蛋白质组学
  • 英文关键词:Cordyceps militaris;;genetic diversity;;molecular genetics;;genomics;;transcriptomics;;proteinomics
  • 中文刊名:SPKX
  • 英文刊名:Food Science
  • 机构:北京市辐射中心;
  • 出版日期:2017-12-12 15:37
  • 出版单位:食品科学
  • 年:2018
  • 期:v.39;No.588
  • 基金:北京市科学技术研究院“萌芽计划”项目(201601);北京市科学技术研究院“青年骨干计划”项目(201511)
  • 语种:中文;
  • 页:SPKX201823048
  • 页数:8
  • CN:23
  • ISSN:11-2206/TS
  • 分类号:330-337
摘要
蛹虫草是虫草属的模式种,具有抗炎、抗氧化、抗肿瘤、提高免疫力等作用,可以实现大规模人工培养,经济和药用价值很高,现已成为食药用菌的研究热点。本文对蛹虫草遗传多样性、分子遗传学和基因组学、转录组学、蛋白质组学方面的研究现状进行了总结,并对蛹虫草的研究前景进行展望。
        Cordyceps militaris(L.) Link is the type species of the genus Cordyceps, with anti-inflammatory, anti-oxidant, anti-tumor and immunoenhancing effects, and it can be artificially cultivated in large scale for high economic and medicinal value. C. militaris has become a hot research subject as an edible and medicinal fungus. This paper summarizes the recent progresses in research on the genetic diversity, molecular genetics, genomics, transcriptomics and proteomics of C. militarisand discusses future prospects.
引文
[1]黄年来,林志彬,陈国良,等.中国食药用菌学[M].上海:上海科学技术文献出版社,2010:1763.
    [2]LIAN Tiantian,YANG Tao,SUN Junde,et al.Variations of SSUrDNA group I introns in different isolates of Cordyceps militaris and the loss of an intron during cross-mating[J].Journal of Microbiology,2014,52(8):659-666.DOI:610.1007/s12275-12014-13681-12274.
    [3]高凌飞,王义祥,翁伯琦.蛹虫草工厂化栽培与系列加工技术研究进展[J].中国农学通报,2014,30(13):93-101.
    [4]张姝,张永杰,SHRESTHA B,等.冬虫夏草菌和蛹虫草菌的研究现状、问题及展望[J].菌物学报,2013,32(4):577-597.DOI:10.13346/j.mycosystema.2013.04.001.
    [5]杨涛,董彩虹.虫草素的研究开发现状与思考[J].菌物学报,2011,30(2):180-190.
    [6]樊慧婷,林洪生.蛹虫草化学成分及药理作用研究进展[J].中国中药杂志,2013,38(15):2549-2552.DOI:10.3969/j.issn.0517-6611.2011.32.041.
    [7]文庭池,查岭生,康冀川,等.蛹虫草研究和开发过程中的一些问题和展望[J].菌物学报,2017,36(1):14-27.DOI:10.13346/j.mycosystema.160199.
    [8]孟泽彬,陈林会,韩近雨,等.蛹虫草化学活性成分的研究进展[J].分子植物育种,2015,13(9):2147-2154.DOI:10.1327/j.mpb.013.002147.
    [9]杜琳,邱芳萍,邢燕,等.蛹虫草菌丝体中SOD提纯工艺及鉴定[J].食用菌学报,2013,20(4):49-54.DOI:10.3969/j.issn.1005-9873.2013.04.012.
    [10]刘晓兰,张雯舒,郑喜群,等.蛹虫草发酵产物新纤溶酶的分离纯化[J].华南理工大学学报(自然科学版),2012,40(5):107-114.DOI:10.3969/j.issn.1000-565X.2012.05.019.
    [11]沈照鹏.虫草主要活性成分分析及指纹图谱研究[D].青岛:中国海洋大学,2012:11-59.
    [12]JOUNG H J,KIM Y S,HWANG J W,et al.Anti-inflammatory effects of extract from Haliotis discus hannai,fermented with Cordyceps militaris,mycelia in RAW264.7 macrophages through TRIFdependent signaling pathway[J].Fish&Shellfish Immunology,2014,38(1):184-189.DOI:10.1016/j.fsi.2014.03.018.
    [13]CHEN Xiaoli,WU Guanghong,HUANG Zhuolie.Structural analysis and antioxidant activities of polysaccharides from cultured Cordyceps militaris[J].International Journal of Biological Macromolecules,2013,58(7):18-22.DOI:10.1016/j.ijbiomac.2013.03.041.
    [14]YANG Shuang,JIN Lu,REN Xiaodong,et al.Optimization of fermentation process of Cordyceps militaris and antitumor activities of polysaccharides in vitro[J].Journal of Food&Drug Analysis,2014,22(4):468-476.DOI:10.1016/j.jfda.2014.01.028.
    [15]LIU Fei,ZHU Zhenyuan,SUN Xiaoli,et al.The preparation of three selenium-containing Cordyceps militaris polysaccharides:characterization and anti-tumor activities[J].Iternational Journal of Biological Macromolecules,2017,99:196-204.DOI:10.1016/j.ijbiomac.2017.02.064.
    [16]XIONG Ying,ZHANG Shuang,XU Linli,et al.Suppression of T-cell activation in vitro and in vivo by cordycepin from Cordyceps militaris[J].Journal of Surgical Research,2013,185(2):912-922.DOI:10.1016/j.jss.2013.06.057.
    [17]DAS S K,MASUDA M,SAKURAI A,et al.Medicinal uses of the mushroom Cordyceps militaris:current state and prospects[J].Fitoterapia,2010,81(8):961-968.DOI:10.1016/j.fitote.2010.07.01.
    [18]REIS F S,BARROS L,CALHELHA R C,et al.The methanolic extract of Cordyceps militaris(L.)Link fruiting body shows antioxidant,antibacterial,antifungal and antihuman tumor cell lines properties[J].Food&Chemical Toxicology,2013,62(6):91-98.DOI:10.1016/j.fct.2013.08.033.
    [19]KANG Chao,WEN Tingchi,KANG Jichuan,et al.Optimization of large-scale culture conditions for the production of cordycepin with Cordyceps militaris by liquid static culture[J].The Scientific World Journal,2014,2014(8):1-15.DOI:10.1155/2014/510627.
    [20]刘桂君,周思静,杨素玲,等.蛹虫草中虫草素的研究进展[J].食品科学,2013,34(21):408-413.DOI:10.7506/spkx1002-6630-201321080.
    [21]周思静,刘桂君,尚宏忠,等.蛹虫草人工培养技术研究进展[J].江苏农业科学,2014,42(7):13-17.DOI:10.3969/j.issn.1002-1302.2014.07.004.
    [22]TANG Jiapeng,LIU Yiting,ZHU Li.Optimization of fermentation conditions and purification of cordycepin from Cordyceps militaris[J].Preparative Biochemistry and Biotechnology,2014,44(1):90-106.DOI:10.1080/10826068.2013.833111.
    [23]燕心慧,齐秋月,汪世华,等.蛹虫草子实体活性成分的分离鉴定[J].菌物学报,2016,35(5):605-610.DOI:10.13346/j.mycosystema.160039.
    [24]卫生部.中华人民共和国卫生部公告(2009年第3号)[J].中华人民共和国卫生部公报,2009(5):21-22.
    [25]国家卫生和计划生育委员会.关于批准塔格糖等6种新食品原料的公告(2014年第10号)[J].中国食品添加剂,2014(5):202-204.DOI:10.3969/j.issn.1006-2513.2014.05.027.
    [26]DONG Caihong,GUO Suping,WANG Wenfeng,et al.Cordyceps industry in China[J].Mycology,2015,6(2):121-129.DOI:10.1080/21501203.2015.1043967.
    [27]董彩虹,李文佳,李增智,等.我国虫草产业发展现状、问题及展望:虫草产业发展金湖宣言[J].菌物学报,2016,35(1):1-15.DOI:10.13346/j.mycosystema.150207.
    [28]SHRESTHA B,ZHANG Weimin,ZHANG Yongjie,et al.The medicinal fungus Cordyceps militaris:research and development[J].Mycological Progress,2012,11(3):599-614.DOI:10.1007/s11557-012-0825-y.
    [29]SHRESHA B,SUNG J M.Notes on Cordyceps species collected from the central region of Nepal[J].Mycobiology,2005,33(4):235-239.
    [30]宋玉溪,赵新利.蛹虫草的研究开发进展[J].河南农业,2007(21):44.DOI:10.3969/j.issn.1006-950X.2007.21.047.
    [31]宋斌,林群英,李泰辉,等.中国虫草属已知种类及其分布[J].菌物研究,2006,4(4):10-26.DOI:10.3969/j.issn.1672-3538.2006.04.004.
    [32]蒋三俊.冬虫夏草及蛹虫草寄主昆虫资源[J].特种经济动植物,2001,4(5):15.DOI:10.3969/j.issn.1001-4713.2001.05.013.
    [33]ZHANG Y J,XU L L,ZHANG S,et al.Genetic diversity of Ophiocordyceps sinensis,a medicinal fungus endemic to the Tibetan Plateau:implications for its evolution and conservation[J].BMCEvolutionary Biology,2009,9(1):1-12.
    [34]SUNG J M,KIM S H,YOON C S,et al.Analysis of genetic relationship of Cordyceps militaris in Korea by random amplified polymorphic DNA[J].Korean Journal of Mycology,1999,27(4):256-273.
    [35]WANG Lei,ZHANG Weimin,HU Bo,et al.Genetic variation of Cordyceps militaris and its allies based on phylogenetic analysis of rDNA ITS sequence data[J].Fungal Diversity,2008,31:147-155.
    [36]YUAN Feng,YU Hong,ZUO Shimei,et al.Development of ESTSSRfor preliminary analysis of genetic diversity of Cordyceps militaris[J].Biochemical Systematics&Ecology,2015,58:126-131.DOI:10.1016/j.bse.2014.11.008.
    [37]李挺,宋斌,林敏.药用真菌蛹虫草遗传多样性的SCoT分析[J].中药材,2016,39(7):1488-1492.DOI:10.13863/j.issn1001-4454.2016.07.011.
    [38]李美娜,吴谢军,李春燕,等.人工栽培蛹虫草退化现象的分子分析[J].菌物系统,2003,22(2):277-282.DOI:10.13346/j.mycosystema.2003.02.019.
    [39]李闽锋,何劲,丁玲,等.不产子实体蛹虫草单孢子分离株的获得及RAPD分析[J].西南农业学报,2007,20(3):547-550.
    [40]丁湖广.菌种退化与老化原因及防止措施[J].特种经济动植物,2006,9(1):39-40.DOI:10.3969/j.issn.1001-4829.2007.03.050.
    [41]林清泉,丘雪红,郑壮丽,等.蛹虫草退化菌株的特征研究[J].菌物学报,2010,29(5):670-677.
    [42]CHEN Anhui,WANG Yulong,SHAO Ying,et al.A novel technique for rejuvenation of degenerated caterpillar medicinal mushroom,Cordyceps militaris(Ascomycetes),a valued traditional Chinese medicine[J].International Journal of Medicinal Mushrooms,2017,19(1):87-91.DOI:10.1615/IntJMedMushrooms.v19.i1.90.
    [43]李美娜.人工栽培蛹虫草性状变异的遗传学分析[D].大连:辽宁师范大学,2003:14-57.
    [44]熊承慧.蛹虫草发育相关的转录组和菌种改良研究[D].上海:中国科学院大学,2013:1-49.
    [45]XIONG Chenghui,XIA Yongliang,ZHENG Peng,et al.Increasing oxidative stress tolerance and subculturing stability of Cordyceps militaris by overexpression of a glutathione peroxidase gene[J].Applied Microbiology&Biotechnology,2013,97(5):2009-2015.DOI:10.1007/s00253-012-4286-7.
    [46]李振刚.分子遗传学[M].4版.北京:科学出版社,2014:1.
    [47]MICHIELSE C B,HOOYKAAS P J J,VAN DEN HONDELC,et al.Agrobacterium-mediated transformation as a tool for functional genomics in fungi[J].Current Genetics,2005,48(1):1-17.DOI:10.1007/s00294-005-0578-0.
    [48]HARWOOD W A.A protocol for high-throughput Agrobacteriummediated barley transformation[J].Methods in Molecular Biology,2014,1099(2):251-260.DOI:10.1007/978-1-62703-715-0_20.
    [49]ZHENG Zhuangli,HUANG Chuanhua,CHAO Li,et al.Agrobacterium tumefaciens-mediated transformation as a tool for insertional mutagenesis in medicinal fungus Cordyceps militaris[J].Fungal Biology,2011,115(3):265-274.DOI:10.1016/j.funbio.2010.12.011.
    [50]JIANG Keqing,HAN Richou.Rhf1 gene is involved in the fruiting body production of Cordyceps militaris fungus[J].Journal of Industrial Microbiology&Biotechnology,2015,42(8):1183-1196.DOI:10.1007/s10295-015-1637-2.
    [51]ZHENG Zhuangli,QIU Xuehong,HAN Richou.Identification of the genes involved in the fruiting body production and Cordycepin formation of Cordyceps militaris fungus[J].Mycobiology,2015,43(1):37-42.DOI:10.5941/MYCO.2015.43.1.37.
    [52]茅文俊,鲍大鹏,周陈力,等.运用基因枪法进行蛹虫草遗传转化的研究[J].园艺学报,2015,42(9):1837-1842.DOI:10.16420/j.issn.0513-353x.2015-0145.
    [53]SANFORD J C.The biolistic process[J].Trends in Biotechnology,1988,6(12):299-302.DOI:10.1016/0167-7799(88)90023-6.
    [54]BERG M A V D,MARUTHACHALAM K.Genetic transformation systems in fungi,volume 1[M].Berlin:Springer International Publishing,2015:129.
    [55]KLOSE R J,BIRD A P.Genomic DNA methylation:the mark and its mediators[J].Trends in Biochemical Sciences,2006,31(2):89-97.DOI:10.1016/j.tibs.2005.12.008.
    [56]MARTIENSSEN R A,COLOT V.DNA methylation and epigenetic inheritance in plants and filamentous fungi[J].Science,2001,293:1070-1074.DOI:10.1126/science.293.5532.1070.
    [57]WANG Yulong,WANG Zhangxun,LIU Chun,et al.Genomewide analysis of DNA methylation in the sexual stage of the insect pathogenic fungus Cordyceps militaris[J].Fungal Biology,2015,119(12):1246-1254.DOI:10.1016/j.funbio.2015.08.017.
    [58]LAW J A,JACOBSEN S E.Establishing,maintaining and modifying DNA methylation patterns in plants and animals[J].Nature Reviews Genetics,2010,11(3):204-220.DOI:10.1038/nrg2719.
    [59]ZHENG Peng,XIA Yongliang,XIAO Guohua,et al.Genome sequence of the insect pathogenic fungus Cordyceps militaris,a valued traditional Chinese medicine[J].Genome Biology,2011,12(11):1-21.DOI:10.1186/gb-2011-12-11-r116.
    [60]汪小艳,荣跃文,徐莉,等.蛹虫草无性型蛹草拟青霉的电泳核型分析[J].安徽农业大学学报,2010,37(4):716-719.
    [61]ZHENG Peng,XIA Yongliang,ZHANG Siwei,et al.Genetics of Cordyceps and related fungi[J].Applied Microbiology&Biotechnology,2013,97(7):2797-2804.DOI:10.1007/s00253-013-4771-7.
    [62]STAATS C C,翹GELA J,GUEDES R L M,et al.Comparative genome analysis of entomopathogenic fungi reveals a complex set of secreted proteins[J].BMC Genomics,2014,15(1):1-18.DOI:10.1186/1471-2164-15-822.
    [63]XIAO Guohua,Ying Shenghua,ZHENG Peng,et al.Genomic perspectives on the evolution of fungal entomopathogenicity in Beauveria bassiana[J].Scientific Reports,2012,2:1-10.DOI:10.1038/srep00483.
    [64]GAO Qiang,JIN Kai,YING Shenghua,et al.Genome sequencing and comparative transcriptomics of the model entomopathogenic fungi Metarhizium anisopliae and M.acridum[J].PLoS Genetics,2011,7(1):1-18.DOI:10.1371/journal.pgen.1001264.
    [65]HU Xiao,ZHANG Yongjie,XIAO Guohua,et al.Genome survey uncovers the secrets of sex and lifestyle in caterpillar fungus[J].Chinese Science Bulletin,2013,58(23):2846-2854.DOI:10.1007/s11434-013-5929-5.
    [66]BECKER K,BEER C,FREITAG M,et al.Genome-wide identification of target genes of a mating-typeα-domain transcription factor reveals functions beyond sexual development[J].Molecular Microbiology,2015,96(5):1002-1022.DOI:10.1111/mmi.12987.
    [67]CONG Weiran,GONG Zhenhua,SHI Dandan,et al.Cloning of mating-type gene MAT1-1 from the caterpillar medicinal mushroom,Cordyceps militaris(Ascomycetes)using TAIL-PCR technology[J].International Journal of Medicinal Mushrooms,2014,16(5):449-461.DOI:10.1615/IntJMedMushrooms.v16.i5.40.
    [68]魏静.蛹虫草交配型基因的克隆及其对子实体形成的影响[D].南京:南京农业大学,2009:27-34.
    [69]莫红丽,叶小舟,吴继明,等.蛹虫草中虫草素差异表达基因cDNA的克隆[J].广东蚕业,2010,44(2):38-46.
    [70]叶小舟,吴继明,莫红丽,等.与虫草素生物合成相关的基因序列:CN101423835[P].2009-05-06[2017-07-03].https://patentscope.wipo.int/search/en/detail.jsf?docId=CN83618368.
    [71]WANG Chengshu,XIA Yongliang,SHANG Yanfang.Identification and use of synthetic gene cluster of cordycepin:WO 2016029802[P].2016-03-03[2017-07-03].https://patentscope.wipo.int/search/en/detail.jsf?docId=WO2016029802.
    [72]DESMOND E,BROCHIER-ARMANET C,FORTRICK P,et al.On the last common ancestor and early evolution of eukaryotes:reconstructing the history of mitochondrial ribosomes[J].Research in Microbiology,2011,162(1):53-70.DOI:10.1016/j.resmic.2010.10.004.
    [73]SUNG G H.Complete mitochondrial DNA genome of the medicinal mushroom Cordyceps militaris(Ascomycota,Cordycipitaceae)[J].Mitochondrial DNA,2015,26(5):789-790.DOI:10.3109/19401736.2013.855754.
    [74]张永杰,郭丽宏,张姝,等.适于蛹虫草遗传多样性研究的线粒体分子标记的筛选[J].微生物学报,2015,55(7):826-833.DOI:10.13343/j.cnki.wsxb.20150064.
    [75]SCHUSTER S C.Next-generation sequencing transforms today’s biology[J].Nature Methods,2008,5(1):16-18.DOI:10.1038/NMETH1156.
    [76]MUTZ K O,HEILKENBRINKER A,L諲NE M,et al.Transcriptome analysis using next-generation sequencing[J].Current Opinion in Biotechnology,2013,24(1):22-30.DOI:10.1016/j.copbio.2012.09.004.
    [77]KEELING P J,BURKI F,WILCOX H M,et al.The marine microbial eukaryote transcriptome sequencing project(MMETSP):illuminating the functional diversity of eukaryotic life in the oceans through transcriptome sequencing[J].PLoS Biology,2014,12(6):1-6.DOI:10.1371/journal.pbio.1001889.
    [78]XIONG Chenghui,XIA Yongliang,ZHENG Peng,et al.Developmental stage-specific gene expression profiling for a medicinal fungus Cordyceps militaris[J].Mycology,2010,1(1):25-66.DOI:10.1080/21501201003674581.
    [79]CHOI J,JUNG W H,KRONSTAD J W.The cAMP/protein kinase A signaling pathway in pathogenic basidiomycete fungi:connections with iron homeostasis[J].Journal of Microbiology,2015,53(9):579-587.DOI:10.1007/s12275-015-5247-5.
    [80]YIN Yalin,YU Guojun,CHEN Yijie,et al.Genome-wide transcriptome and proteome analysis on different developmental stages of Cordyceps militaris[J].PLoS ONE,2012,7(12):1-15.DOI:10.1371/journal.pone.0051853.
    [81]RADONIA,THULKE S,MACKAY I M,et al.Guideline to reference gene selection for quantitative real-time PCR[J].Biochemical&Biophysical Research Communications,2004,313(4):856-862.DOI:10.1016/j.bbrc.2003.11.177.
    [82]LIAN Tiantian,YANG Tao,LIU Guijun,et al.Reliable reference gene selection for Cordyceps militaris gene expression studies under different developmental stages and media[J].FEMS Microbiology Letters,2014,356(1):97-104.DOI:10.1111/1574-6968.12492.
    [83]WILKINS M R,PASQUALI C,APPEL R D,et al.From proteins to proteomes:large scale protein identification by two-dimensional electrophoresis and amino acid analysis[J].Nature Biotechnology,1996,14(1):61-65.
    [84]ANDERSON N L,ANDERSON N G.Proteome and proteomics:new technologies,new concepts,and new words[J].Electrophoresis,1998,19(11):1853-1861.
    [85]尹稳,伏旭,李平.蛋白质组学的应用研究进展[J].生物技术通报,2014(1):32-38.
    [86]王雅玲,孙力军,赵轶男,等.蛹虫草大米培养残基中虫草素提取方法的优化研究[J].安徽农业科学,2009,37(8):3364-3366.DOI:10.3969/j.issn.0517-6611.2009.08.010.
    [87]方尚瑜,贾志华,张霞,等.碳氮源对液体培养蛹虫草生物合成虫草多糖的影响[J].食品科学,2013,34(13):165-169.DOI:10.7506/spkx1002-6630-201313036.
    [88]MAO Xianbing,EKSRIWONG T,CHAUVATCHARIN S,et al.Optimization of carbon source and carbon/nitrogen ratio for cordycepin production by submerged cultivation of medicinal mushroom Cordyceps militaris[J].Process Biochemistry,2005,40(5):1667-1672.DOI:10.1016/j.procbio.2004.06.046.
    [89]李会平,魏晓鹏,岳素红.桑天牛幼虫致病性白僵菌菌株退化的RAPD分析[J].蚕业科学,2013,39(2):226-230.DOI:10.13441/j.cnki.cykx.2013.02.008.
    [90]LI Lin,HU Xiao,XIA Yongliang,et al.Linkage of oxidative stress and mitochondrial dysfunctions to spontaneous culture degeneration in Aspergillus nidulans[J].Molecular&Cellular Proteomics,2014,13(2):449-461.DOI:10.1074/mcp.M113.028480.
    [91]沈爱喜,胡中娥,李小云,等.北冬虫夏草菌种退化原因及防控方法[J].食用菌,2013,35(4):28-29.DOI:10.3969/j.issn.1000-8357.2013.04.014.
    [92]LI A M,BEGIN M,KOKUREWICZ K,et al.Inheritance of strain instability(sectoring)in the commercial button mushroom,Agaricus bisporus[J].Applied&Environmental Microbiology,1994,60(7):2384-2388.
    [93]RYAN M J,BRIDGE P D,SMITH D,et al.Phenotypic degeneration occurs during sector formation in Metarhizium anisopliae[J].Journal of Applied Microbiology,2002,93(1):163-168.
    [94]CUI Jiandong,ZHANG Baozhu.Comparison of culture methods on exopolysaccharide production in the submerged culture of Cordyceps militaris and process optimization[J].Letters in Applied Microbiology,2011,52(2):123-128.DOI:10.1111/j.1472-765X.2010.02987.x.
    [95]何洋,刘林德,赵彦宏,等.蛹虫草优化培养研究进展[J].鲁东大学学报(自然科学版),2011,27(1):64-70.DOI:10.3969/j.issn.1673-8020.2011.01.016.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700