奶牛乳房炎病原菌金黄色葡萄球菌的分离鉴定及耐药性分析
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摘要
为研究江苏某奶牛场金黄色葡萄球菌的耐药特点,本试验通过MALDI Biotyper系统对8株金黄色葡萄球菌疑似株进行鉴定和耐药性试验。结果显示,8株金黄色葡萄球菌疑似株均为金黄色葡萄球菌,其对青霉素、链霉素、环丙沙星的耐药率均为100%;其次,对阿莫西林的耐药率为75%;极少数对头孢氨苄、庆大霉素、丁胺卡那、卡那霉素耐药;而对四环素、多西环素、克林霉素均敏感。结果表明,该奶牛场金黄色葡萄球菌的分离率为14.55%(8/55),且均呈现多重耐药。
In order to investigate the drug resistance characteristics of Staphylococcus aureus isolated from bovine mastitis in Jiangsu, totally 8 suspected S.aureus strains isolated from 55 milk samples of bovine mastitis were identified by MALDI Biotyper system. Meanwhile, the antibiotic susceptibility test was also carried out. The results showed that 8 suspected S.aureus strains were identified as Staphylococcus aureus. All S.aureus isolates were resistant to penicillin, streptomycin and ciprofloxacin. About 75% of the S.aureus were resistant to amoxicillin and only few isolates were resistant to cephalexin, gentamicin, amikacin and kanamycin. And all S.aureus isolates were susceptible to tetracycline, doxycycline and clindamycin. The results showed that isolation rate of S.aureus was14.55%(8/55), and the 8 S.aureus isolates showed multi-drug resistance. The results can provide theoretical basis for standard application of antibacterial drugs in clinical practice.
In order to investigate the drug resistance characteristics of Staphylococcus aureus isolated from bovine mastitis in Jiangsu, totally 8 suspected S.aureus strains isolated from 55 milk samples of bovine mastitis were identified by MALDI Biotyper system. Meanwhile, the antibiotic susceptibility test was also carried out. The results showed that 8 suspected S.aureus strains were identified as Staphylococcus aureus. All S.aureus isolates were resistant to penicillin, streptomycin and ciprofloxacin. About 75% of the S.aureus were resistant to amoxicillin and only few isolates were resistant to cephalexin, gentamicin, amikacin and kanamycin. And all S.aureus isolates were susceptible to tetracycline, doxycycline and clindamycin. The results showed that isolation rate of S.aureus was14.55%(8/55), and the 8 S.aureus isolates showed multi-drug resistance. The results can provide theoretical basis for standard application of antibacterial drugs in clinical practice.
引文
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[2]Patel R.MALDI-TOF MS for the diagnosis of infectious diseases[J].Clinical Chemistry,2015,61(1):100-111.
[3]田晓英,王军,于忠娜,等,天津地区乳房炎奶样中金黄色葡萄球菌耐药性研究[J].现代食品科技,2017,10:208-216.
[4]田会丽,袁筠,廖超,等.石河子地区乳源性金黄色葡萄球菌毒力及耐药性的研究[J].现代食品科技,2017,9:265-270.
[5]章慧,戴春风,韩奕奕.上海地区奶牛的乳房炎金黄色葡萄球菌耐药性[J].乳业科学与技术,2018,41(2):1-4.
[6]Anderson,K.L.,Lyman,R.,Moury,K.,et al.Molecular epidemiology of Staphylococcus aureus mastitis in dairy heifers[J].Journal of Dairy Science,2012,9:4921-4930.
[7]高潮,刘国庆,赵莉莉,等.上海地区奶牛乳房炎金黄色葡萄球菌基因分型与耐药性研究[J].上海交通大学学报(农业科学版),2014,32(1):53-58.
[8]刘敬兰,刘继超,姜铁民,等.北京某牛场奶牛乳房炎金黄色葡萄球菌的分离鉴定与药敏试验[J].中国奶牛,2015,7:39-42.
[9]王冰,刘明春,吴聪明,等.呼和浩特地区奶牛乳房炎金黄色葡萄球菌的耐药性调查[J].中国兽医杂志,2007,43(3):30-32.
[10]张倩,屈勇刚,于会举,等.石河子地区奶牛乳房炎源金黄色葡萄球菌的分离鉴定及耐药性分析[J].黑龙江畜牧兽医,2017,2:102-105+291.
[11]燕霞,陈俭,张丽佳,等.浙江省主要奶牛养殖区乳房炎致病菌金黄色葡萄球菌的耐药性[J].福建农林大学学报(自然科学版),2012,41(6):633-636.
[12]Wang H,Bi C,Wang Y,et al.Selenium ameliorates Staphylococcus aureus-induced inflammation in bovine mammary epithelial cells by inhibiting activation of TLR2,NF-κB and MAPK signaling pathways[J].BMC Vet Res,2018,14(1):197-205.
[13]Ma J,Zhu S,Guo Y,et al.Selenium Attenuates Staphylococcus aureus Mastitis in Mice by Inhibiting the Activation of the NALP3Inflammasome and NF-κB/MAPK Pathway[J].Biol Trace Elem Res,2018,https://doi.org/10.1007/s12011-018-1591-8.