鱼腥草根及地上部分乙醇提取物抑菌活性和化学成分的分析
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摘要
【目的】考察鱼腥草(Houttuynia cordata)根和地上部分乙醇提取物的抑菌效果和其中化学成分的差异。【方法】通过乙醇回流萃取制备鱼腥草根和地上部分的乙醇提取物,采用涂布平板法和96孔板法测定两者抑菌活性,并结合气相色谱-质谱法(GC-MS)对两种乙醇提取物的成分进行检测分析。【结果】鱼腥草根乙醇提取物对金黄色葡萄球菌(Staphylococcus aureus)、大肠杆菌(Escherichia coli)、枯草芽孢杆菌(Bacillus subitilis)、青霉菌(Penicillium notatum)、黑曲霉菌(Aspergillus niger)和酿酒酵母菌(Saccharomyces cerevisiae)的最低抑菌浓度分别为0.068,0.250,0.125,0.125,0.004和0.250g·mL~(-1);地上部分乙醇提取物对上述实验菌的最低抑菌浓度分别为0.068,0.034,0.068,0.125,0.001和0.125g·mL~(-1)。GC-MS分析结果显示,地上部分乙醇提取物主要含有具有抑菌活性的成分亚麻酸、棕榈酸、5-甲基-2,4-(1H,3H)-嘧啶二酮、磷酸三乙酯、2,4-二叔丁基苯酚和叶绿醇;而根乙醇提取物中仅发现抑菌成分亚麻酸和棕榈酸。【结论】鱼腥草地上部分乙醇提取物的抑菌效果优于根乙醇提取物,且抑菌成分的种类数及含量也高于后者。
[Purposes]To explore the antimicrobial activity and chemical components of the root and aerial part ethanol extracts from H.cordata.[Methods]The ethanol extracts of root and aerial part from H.cordata were prepared with heat reflux extraction by ethanol solvent.The antimicrobial activity was then determined by the plate coated method and 96-well plate method.Meanwhile the chemical components of ethanol extracts were detected by using gas chromatography-mass spectrometry(GC-MS)technology.[Findings]The minimum inhibitory concentration(MIC)of root extract against Staphylococcus aureus,Escherichia coli,Bacillus subitilis,Penicillium notatum,Aspergillus niger,and Saccharomyces cerevisiae were 0.068,0.250,0.125,0.125,0.004,and 0.250 g·mL~(-1),respectively.The MIC of aerial part extract against the test bacteria were 0.068,0.034,0.068,0.125,0.001,and 0.125 g·mL~(-1),respectively.The results obtained by GC-MS revealed that the root and aerial part extracts consisted of various components with antimicrobial activity,including 9,12,15-octadecatrienoic acid,(Z,Z,Z)-and n-hexadecanoic acid,thymine,triethyl phosphate,2,4-di-tert-butylphenol,and phytol.However,only two antibacterial constituents,9,12,15-octadecatrienoic acid,(Z,Z,Z)-and n-hexadecanoic acid,were found in the root extract.[Conclusions]The antimicrobial activity of aerial part extract was stronger than root extract,moreover,the types and contents of antibacterial constituents were obviously increased in the aerial part extract than root extract.
[Purposes]To explore the antimicrobial activity and chemical components of the root and aerial part ethanol extracts from H.cordata.[Methods]The ethanol extracts of root and aerial part from H.cordata were prepared with heat reflux extraction by ethanol solvent.The antimicrobial activity was then determined by the plate coated method and 96-well plate method.Meanwhile the chemical components of ethanol extracts were detected by using gas chromatography-mass spectrometry(GC-MS)technology.[Findings]The minimum inhibitory concentration(MIC)of root extract against Staphylococcus aureus,Escherichia coli,Bacillus subitilis,Penicillium notatum,Aspergillus niger,and Saccharomyces cerevisiae were 0.068,0.250,0.125,0.125,0.004,and 0.250 g·mL~(-1),respectively.The MIC of aerial part extract against the test bacteria were 0.068,0.034,0.068,0.125,0.001,and 0.125 g·mL~(-1),respectively.The results obtained by GC-MS revealed that the root and aerial part extracts consisted of various components with antimicrobial activity,including 9,12,15-octadecatrienoic acid,(Z,Z,Z)-and n-hexadecanoic acid,thymine,triethyl phosphate,2,4-di-tert-butylphenol,and phytol.However,only two antibacterial constituents,9,12,15-octadecatrienoic acid,(Z,Z,Z)-and n-hexadecanoic acid,were found in the root extract.[Conclusions]The antimicrobial activity of aerial part extract was stronger than root extract,moreover,the types and contents of antibacterial constituents were obviously increased in the aerial part extract than root extract.
引文
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[18]ABEBE A,SENDEK A,AYALEW S,et al.Copper(II)mixed-ligand complexes containing 1,10-phenanthroline,adenine and thymine:Synthesis,characterization and antibacterial activities[J].Chemistry International,2017,3(3):230-239.
[19]LIU H,CHEN Q,SONG L,et al.Ag-doped antibacterial porous materials with slow release of silver ions[J].Journal of Non-Crystalline Solids,2008,354(12/13):1314-1317.
[20]RIDA S M,SOLIMAN F S G,BADAWEY E A M,et al.Benzimidazole condensed ring systems.I:Syntheses and biological investigations of some substituted Pyrido[1,2-a]benzimidazoles[J].Journal of Heterocyclic Chemistry,1988,25(4):1087-1093.
[21]PADMAVATHI A R,BAKKIYAYAJ D,THAJUDDINN,et al.Effect of 2,4-di-tert-butylphenol on growth and biofilm formation by an opportunistic fungus Candida albicans[J].Biofouling,2015,31(7):565-574.
[22]ELUCHIE C N,NJOKU N,ALAGBAOSO S O,et al.Effect of phytol on bacterial isolates from grilled meat[J].European Journal of English Language and Literature Studies,2015,3(5):1-14.
[23]LEE W,WOO E R,LEE D G.Phytol has antibacterial property by inducing oxidative stress response in Pseudomonas aeruginosa[J].Free Radical Research,2016,50(12):1309-1318.
[2]陈婧,方建国,吴方建,等.鱼腥草抗炎药理作用机制的研究进展[J].中草药,2014,45(2):284-289.CHEN J,FANG J G,WU F J,et al.Progress of study on Anti-inflammatory Pharmacological Mechanism of Houttuynia Cordata Thunb[J].Chinese Traditional and Herbal Drugs,2014,45(2):284-289.
[3]ZENG H Y,JIANG L J,ZHANG Y C.Chemical constituents of volatile oil from Houttuynia cordata Thunb[J].Journal of Plant Resources&Environment,2003,12(3):50-52.
[4]CHIO W K H,PHOON M C,PUTTI T,et al.Evaluation of antiviral activities of Houttuynia cordata Thunb.extract,quercetin,quercetrin and cinanserin on murine coronavirus and dengue virus infection[J].Asian Pacific Journal of Tropical Medicine,2016,9(1):1-7.
[5]YANG X Y,SHI T,DU G,et al.iTRAQ-Based proteomics revealed the bactericidal mechanism of sodium new houttuyfonate against Streptococcus pneumoniae[J].Journal of Agricultural&Food Chemistry,2016,64(32):6375-6382.
[6]JUN L,ZHAO F.Anti-inflammatory functions of Houttuynia cordata Thunb.and its compounds:aperspective on its potential role in rheumatoid arthritis[J].Experimental&Therapeutic Medicine,2015,10(1):3-6.
[7]AHN J,CHAE H S,CHIN Y W,et al.Alkaloids from aerial parts of Houttuynia cordata and their anti-inflammatory activity[J].Bioorganic&Medicinal Chemistry Letters,2017,27(12):2807-2811.
[8]JIN M C,NHO K J,KIM H S,et al.An ethyl acetate fraction derived from Houttuynia cordata extract inhibits the production of inflammatory markers by suppressing NF-кBand MAPK activation in lipopolysaccharide-stimulated RAW 264.7 macrophages[J].Bmc Complementary&Alternative Medicine,2014,14(1):234-244.
[9]国家药典委员会.中国药典(一部)[M].北京:中国医药科学出版社,2010:208-209.Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia,VolΙ[M].Beijing:China Medical Science Press,2010:208-209.
[10]KRAML M,ROBINSON W T.Identification and antimicrobial activity of combined extract fromAzadirachta indica and Ocimum sanctum[J].The Israeli Journal of Aquaculture-Bamidgeh,2010,62(2):85-89.
[11]ZHANG J,WU X J,LUO Z G,et al.Study on the change of chemical components and antibacterial activity of Houttuynia cordata Thunb during desiccation[J].Food Science&Technology,2007,32(4):89-92.
[12]MING F.Research on antibacterial activity in vitro of the Houttuynia cordata Thunb injection[J].Journal of Anhui Agricultural Sciences,2007,35(8):2264-2265.
[13]杨勇,张凤英,陈岑.PDA培养基改良配方的研究[J].酿酒科技,2012,214(4):29-31.YANG Y,ZHANG F Y,CHEN Q.Study on the improved formula of PDA culture medium[J].Liquor-making Science&Technology,2012,214(4):29-31.
[14]CHOI W H.Evaluation of anti-tubercular activity of linolenic acid and conjugated-linoleic acid as effective inhibitors against Mycobacterium tuberculosis[J].Asian Pacific Journal of Tropical Medicine,2016,9(2):121-125.
[15]LEE J Y,KIM Y S,SHIN D H.Antimicrobial synergistic effect of linolenic acid and monoglyceride against Bacillus cereus and Staphylococcus aureus[J].Journal of Agricultural&Food Chemistry,2002,50(7):2193-2199.
[16]OBONYO M,ZHANG L,THAMPHIWATANA S,et al.Antibacterial activities of liposomal linolenic acids against antibiotic-resistant Helicobacter pylori[J].Molecular Pharmaceutics,2012,9(9):2677-2685.
[17]李晶,阮维斌,陈永智,等.天然脂肪酸类物质对温室连作黄瓜和番茄幼苗生长的影响[J].农业环境科学学报,2008,27(3):1022-1028.LI J,YUAN W B,CHEN Y Z,et al.Effects of Natural Fatty Acids on the Growth of Continuous Cropping Cucumber and Tomato Seedlings in Greenhouse[J].Journal of Agro-Environment Science 2008,27(3):1022-1028.
[18]ABEBE A,SENDEK A,AYALEW S,et al.Copper(II)mixed-ligand complexes containing 1,10-phenanthroline,adenine and thymine:Synthesis,characterization and antibacterial activities[J].Chemistry International,2017,3(3):230-239.
[19]LIU H,CHEN Q,SONG L,et al.Ag-doped antibacterial porous materials with slow release of silver ions[J].Journal of Non-Crystalline Solids,2008,354(12/13):1314-1317.
[20]RIDA S M,SOLIMAN F S G,BADAWEY E A M,et al.Benzimidazole condensed ring systems.I:Syntheses and biological investigations of some substituted Pyrido[1,2-a]benzimidazoles[J].Journal of Heterocyclic Chemistry,1988,25(4):1087-1093.
[21]PADMAVATHI A R,BAKKIYAYAJ D,THAJUDDINN,et al.Effect of 2,4-di-tert-butylphenol on growth and biofilm formation by an opportunistic fungus Candida albicans[J].Biofouling,2015,31(7):565-574.
[22]ELUCHIE C N,NJOKU N,ALAGBAOSO S O,et al.Effect of phytol on bacterial isolates from grilled meat[J].European Journal of English Language and Literature Studies,2015,3(5):1-14.
[23]LEE W,WOO E R,LEE D G.Phytol has antibacterial property by inducing oxidative stress response in Pseudomonas aeruginosa[J].Free Radical Research,2016,50(12):1309-1318.