浙江省甘薯上发现瓜类细菌性果斑病菌
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摘要
通过对浙江宁海县疑似甘薯茎腐病等检疫性病害的调查,在甘薯病株上分离纯化获得能够引起烟草过敏反应的4株分离物,通过菌落形态观察、革兰氏染色反应、16S rRNA序列分析以及致病性测定等细菌学测定,将这4株菌株都鉴定为西瓜噬酸菌(Acidovorax citrulli,原Acidovorax avenae subsp.citrulli),序列同源性高达99.00%～100.00%。瓜类细菌性果斑病菌为我国的检疫性有害生物,务必引起高度重视,以保障浙江重要经济作物西甜瓜产业的健康发展。
Bacterial stem and root rot of sweet potato is an important quarantine disease. In the current study, four isolates of suspected diseased sweet potato induced a hypersensitive response on tobacco plants. These isolates were then identified into Acidovorax citrulli(formerly Acidovorax avenae subsp. citrulli) based on morphology, Gram's stain reaction, 16 S rRNA sequence and pathogenicity analysis. Results here showed that the sequence homology of the novel isolates was as high as 99.00%-100.00% compared with A. citrulli, the bacterial pathogen of bacterial fruit blotch of cucurbits, which was the key quarantine pathogen of China. The current report indicated the importance of disease management for efficient controlling of bacterial fruit blotch of watermelon in Zhejiang Province.
Bacterial stem and root rot of sweet potato is an important quarantine disease. In the current study, four isolates of suspected diseased sweet potato induced a hypersensitive response on tobacco plants. These isolates were then identified into Acidovorax citrulli(formerly Acidovorax avenae subsp. citrulli) based on morphology, Gram's stain reaction, 16 S rRNA sequence and pathogenicity analysis. Results here showed that the sequence homology of the novel isolates was as high as 99.00%-100.00% compared with A. citrulli, the bacterial pathogen of bacterial fruit blotch of cucurbits, which was the key quarantine pathogen of China. The current report indicated the importance of disease management for efficient controlling of bacterial fruit blotch of watermelon in Zhejiang Province.
引文
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[2] 蔡学清,黄月英,杨建珍,等.福建省西瓜细菌性果斑病的病原鉴定[J].福建农林大学学报(自然科学版),2005,34(4):434-437.CAI X Q,HUANG Y Y,YANG J Z,et al.Pathogen identification of bacterial fruit blotch of watermelon in Fujian[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition),2005,34(4):434-437.(in Chinese with English abstract)
[3] 张敏荣,余继华,卢璐,等.台州新发现甘薯茎腐病[J].植物检疫,2016,30(3):84-86.ZHANG M R,YU J H,LU L,et al.Stem rot of sweet potato newly discovered in Taizhou[J].Plant Quarantine,2016,30(3):84-86.(in Chinese with English abstract)
[4] 徐玉恒,张永涛,陈香艳,等.春甘薯西瓜套作高效种植模式[J].山东农业科学,2005,37(3):40.XU Y H,ZHANG Y T,CHEN X Y,et al.High efficiency planting model of spring sweet potato watermelon intercropping[J].Shandong Agricultural Sciences,2005,37(3):40.(in Chinese)
[5] 张则林.西瓜套种甘薯高效栽培模式[J].河北农业科技,2007(3):6.ZHANG Z L.High-efficiency cultivation mode about interplanting of watermelon and sweet potato[J].Modern Rural Science and Technology,2007(3):6.(in Chinese)
[6] 明华.甘薯西瓜套种技术[J].农村实用技术,2010(5):47.MING H.Interplanting technique of sweet potato and watermelon[J].Rural Practical Technology,2010(5):47.(in Chinese)
[7] 赵廷昌,孙福在,王兵万,等.哈密瓜细菌性果斑病病原菌鉴定[J].植物病理学报,2001,31(4):357-364.ZHAO T C,SUN F Z,WANG B W,et al.Pathogen identification of Hami melon bacterial fruit blotch[J].Acta Phytopathologica Sinica,2001,31(4):357-364.(in Chinese with English abstract)
[8] 方中达.植病研究方法[M].3版.北京:中国农业出版社,1998.
[9] WALCOTT R R,GITAITIS R D.Detection of acidovorax avenae subsp.citrulli in watermelon seed using immunomagnetic separation and the polymerase chain reaction[J].Plant Disease,2000,84(4):470-474.
[10] 徐福寿,王笑,谢关林,等.免疫捕捉PCR法检测西瓜种子带细菌性果斑病菌[J].果树学报,2008,25(2):215-218.XU F S,WANG X,XIE G L,et al.Detection of Acidovorax avenae subsp.citrulli from seeds of watermelon by immuno-capture PCR[J].Journal of Fruit Science,2008,25(2):215-218.(in Chinese with English abstract)
[11] 冯建军,许勇,李健强,等.免疫凝聚试纸条和TaqMan探针实时荧光PCR检测西瓜细菌性果斑病菌比较研究[J].植物病理学报,2006,36(2):102-108.FENG J J,XU Y,LI J Q,et al.Comparison of immunoStrip and real-time fluorescent PCR (TaqMan) for detection of Acidovorax avenae subsp.citrulli,the causal agent of bacterial fruit blotch of watermelon[J].Acta Phytopathologica Sinica,2006,36(2):102-108.(in Chinese with English abstract)
[12] 陈新垲.西瓜细菌性果斑病菌的PCR检测[D].福州:福建农林大学,2010.CHEN X K.Detection the pathogen of bacterial fruit blotch of watermelon by polymerase chain reaction[D].Fuzhou:Fujian Agriculture and Forestry University,2010.(in Chinese with English abstract)
[13] 沈肖玲,林钗,钱俊婷,等.甘薯茎腐病症状及其病原鉴定[J].植物病理学报,2018,48(1):25-34.SHEN X L,LIN C,QIAN J T,et al.Characterization of stem and root rot symptoms of sweet potato and the causal pathogen of the disease[J].Acta Phytopathologica Sinica,2018,48(1):25-34.(in Chinese with English abstract)
[14] 邢东光.西瓜细菌性果斑病发生与防治[J].北方园艺,2002(3):69.XING D G.The occurance and prevention of bacterical fruit blotch of watermelon[J].Northern Horticulture,2002(3):69.(in Chinesse )
[15] 孟昭军,郭卜,吴连凯,等.西瓜、厚皮甜瓜果斑病防治进展[J].蔬菜,2004(3):22-23.MENG Z J,GUO B,WU L K,et al.The progresses in prevention and treatment of spot disease of watermelon and thick-skinned melon[J].Vegetables,2004(3):22-23.(in Chinese)
[2] 蔡学清,黄月英,杨建珍,等.福建省西瓜细菌性果斑病的病原鉴定[J].福建农林大学学报(自然科学版),2005,34(4):434-437.CAI X Q,HUANG Y Y,YANG J Z,et al.Pathogen identification of bacterial fruit blotch of watermelon in Fujian[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition),2005,34(4):434-437.(in Chinese with English abstract)
[3] 张敏荣,余继华,卢璐,等.台州新发现甘薯茎腐病[J].植物检疫,2016,30(3):84-86.ZHANG M R,YU J H,LU L,et al.Stem rot of sweet potato newly discovered in Taizhou[J].Plant Quarantine,2016,30(3):84-86.(in Chinese with English abstract)
[4] 徐玉恒,张永涛,陈香艳,等.春甘薯西瓜套作高效种植模式[J].山东农业科学,2005,37(3):40.XU Y H,ZHANG Y T,CHEN X Y,et al.High efficiency planting model of spring sweet potato watermelon intercropping[J].Shandong Agricultural Sciences,2005,37(3):40.(in Chinese)
[5] 张则林.西瓜套种甘薯高效栽培模式[J].河北农业科技,2007(3):6.ZHANG Z L.High-efficiency cultivation mode about interplanting of watermelon and sweet potato[J].Modern Rural Science and Technology,2007(3):6.(in Chinese)
[6] 明华.甘薯西瓜套种技术[J].农村实用技术,2010(5):47.MING H.Interplanting technique of sweet potato and watermelon[J].Rural Practical Technology,2010(5):47.(in Chinese)
[7] 赵廷昌,孙福在,王兵万,等.哈密瓜细菌性果斑病病原菌鉴定[J].植物病理学报,2001,31(4):357-364.ZHAO T C,SUN F Z,WANG B W,et al.Pathogen identification of Hami melon bacterial fruit blotch[J].Acta Phytopathologica Sinica,2001,31(4):357-364.(in Chinese with English abstract)
[8] 方中达.植病研究方法[M].3版.北京:中国农业出版社,1998.
[9] WALCOTT R R,GITAITIS R D.Detection of acidovorax avenae subsp.citrulli in watermelon seed using immunomagnetic separation and the polymerase chain reaction[J].Plant Disease,2000,84(4):470-474.
[10] 徐福寿,王笑,谢关林,等.免疫捕捉PCR法检测西瓜种子带细菌性果斑病菌[J].果树学报,2008,25(2):215-218.XU F S,WANG X,XIE G L,et al.Detection of Acidovorax avenae subsp.citrulli from seeds of watermelon by immuno-capture PCR[J].Journal of Fruit Science,2008,25(2):215-218.(in Chinese with English abstract)
[11] 冯建军,许勇,李健强,等.免疫凝聚试纸条和TaqMan探针实时荧光PCR检测西瓜细菌性果斑病菌比较研究[J].植物病理学报,2006,36(2):102-108.FENG J J,XU Y,LI J Q,et al.Comparison of immunoStrip and real-time fluorescent PCR (TaqMan) for detection of Acidovorax avenae subsp.citrulli,the causal agent of bacterial fruit blotch of watermelon[J].Acta Phytopathologica Sinica,2006,36(2):102-108.(in Chinese with English abstract)
[12] 陈新垲.西瓜细菌性果斑病菌的PCR检测[D].福州:福建农林大学,2010.CHEN X K.Detection the pathogen of bacterial fruit blotch of watermelon by polymerase chain reaction[D].Fuzhou:Fujian Agriculture and Forestry University,2010.(in Chinese with English abstract)
[13] 沈肖玲,林钗,钱俊婷,等.甘薯茎腐病症状及其病原鉴定[J].植物病理学报,2018,48(1):25-34.SHEN X L,LIN C,QIAN J T,et al.Characterization of stem and root rot symptoms of sweet potato and the causal pathogen of the disease[J].Acta Phytopathologica Sinica,2018,48(1):25-34.(in Chinese with English abstract)
[14] 邢东光.西瓜细菌性果斑病发生与防治[J].北方园艺,2002(3):69.XING D G.The occurance and prevention of bacterical fruit blotch of watermelon[J].Northern Horticulture,2002(3):69.(in Chinesse )
[15] 孟昭军,郭卜,吴连凯,等.西瓜、厚皮甜瓜果斑病防治进展[J].蔬菜,2004(3):22-23.MENG Z J,GUO B,WU L K,et al.The progresses in prevention and treatment of spot disease of watermelon and thick-skinned melon[J].Vegetables,2004(3):22-23.(in Chinese)