超高效液相色谱/串联质谱同步测定水中多种亚硝胺类物质
|
摘要
饮用水中的亚硝胺类消毒副产物因其毒性强,近年来日益受到广泛关注。以8种亚硝胺为对象,针对仪器分析中环境基质干扰和多种物质同时分析的难点,利用一根活性炭固相萃取(AC-SPE)柱实现同时富集,并结合不同的前处理纯化方法,有效消除了分析过程中基质对响应信号的抑制作用,建立了UPLC-ESI-MS/MS分析方法。8种亚硝胺物质的回收率达到52.2%～113%,相对标准偏差低于18%,方法检出限(MDLs)在0.2～0.9 ng/L,能满足实际的水质检测需求。
Due to their high carcinogenic potency, disinfection byproducts of nitrosamines in drinking water have raised great public concerns. A sensitive and specific method for simultaneous determination of eight nitrosamines in water matrices by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry(UPLC-ESI MS/MS) was developed. An activated carbon cartridge was adopted for simultaneous extraction of nitrosamines, and matrix suppression was effectively reduced by optimizing cleanup procedure. The recoveries of eight nitrosamines were 52.2%～113% in various water samples, and method detection limits were in the range of 0.2～0.9 ng/L. This method was applicable for determining actual environmental water samples.
Due to their high carcinogenic potency, disinfection byproducts of nitrosamines in drinking water have raised great public concerns. A sensitive and specific method for simultaneous determination of eight nitrosamines in water matrices by ultra-performance liquid chromatography-electrospray ionization tandem mass spectrometry(UPLC-ESI MS/MS) was developed. An activated carbon cartridge was adopted for simultaneous extraction of nitrosamines, and matrix suppression was effectively reduced by optimizing cleanup procedure. The recoveries of eight nitrosamines were 52.2%～113% in various water samples, and method detection limits were in the range of 0.2～0.9 ng/L. This method was applicable for determining actual environmental water samples.
引文
[1] HRUDEY S E, CHARROIS J W A. Disinfection by-products and human health[M]. London: IWA Publishing, 2012.
[2] IARC Working Group on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. N-Nitrosodimethylamine in IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans[M]. Switzerland: World Health Organization, 1978: 125-175.
[3] JOBB D B, HUNSINGER R, MERESZ O, et al. A study of the occurrence and inhibition of formation of N-nitrosodimethylamine(NDMA) in the Ohsweken water supply[C]. Winnipeg: Proceedings of the Fifth National Conference on Drinking Water, 1992.
[4] BARRETT S, HWANG C, GUO Y C, et al. Occurrence of NDMA in drinking water: North American survey, 2001-2003[C]. Anaheim: Proceedings of American Water Works Association Annual Conference and Exhibition, 2003.
[5] CHARROIS J W A, BOYD J M, FROESE K L, et al. Occurrence of N-nitrosamines in Alberta public drinking-water distribution systems[J]. Journal of Environmental Engineering & Science, 2007, 6(1): 103-114.
[6] ASAMI M, OYA M, KOSAKA K. A nationwide survey of NDMA in raw and drinking water in Japan[J]. Science of the Total Environment, 2009(11): 3540-3545.
[7] BEI E, SHU Y Y, LI S X, et al. Occurrence of nitrosamines and their precursors in drinking water systems around mainland China[J]. Water Research, 2016, 50(13):168-175.
[8] MEHMET A, SEVKET A. Simultaneous determination of aliphatic and aromatic amines in water and sediment samples by ion-pair extraction and gas chromatography-mass spectrometry[J]. Journal of Chromatography A, 2006(1): 88-94.
[9] PLANAS C, PALACIOS O, VENTURA F, et al. Analysis of nitrosamines in water by automated SPE and isotope dilution GC/HRMS-Occurrence in the different steps of a drinking water treatment plant, and in chlorinated samples from a reservoir and a sewage treatment plant effluent[J]. Talanta, 2008, 76(4): 906-913.
[10] KRAUSS M, HOLLENDER J. Analysis of nitrosamines in wastewater: Exploring the trace level quantification capabilities of a hybrid linear ion trap/orbitrap massspectrometer[J]. Analytical Chemistry, 2008, 80(3): 834-842.
[11] MUNCH J W, BESSETT M V. Method 521: Determination of nitrosamines in drinking water by solid phase extraction and capillary column gas chromatography with large volume injection and chemical ionization tandem mass spectrometry (MS/MS)[Z]. 2004.
[12] ZHAO Y Y, BOYD J, HRUDEY S E, et al. Characterization of new nitrosamines in drinking water using liquid chromatography tandem mass spectrometry[J]. Environmental Science & Technology, 2006, 40 (24): 7636-7641.
[13] WANG W F, HU J Y, YU J W, et al. Determination of N-nitrosodimethylamine in drinking water by UPLC-MS/MS[J]. Journal of Environmental Sciences, 2010, 22(10): 1508-1512.
[14] PLUMLEE M H, LOPEZ-MESAS M, HEIDLBERGER A, et al. N-nitrosodimethylamine (NDMA) removal by reverse osmosis and UV treatment and analysis via LC-MS/MS[J]. Water Research, 2008, 42 (1-2): 347-355.Simultaneous Determination of Multiple Nitrosamines in Drinking Water by UPLC/MS-MS
[2] IARC Working Group on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. N-Nitrosodimethylamine in IARC monographs on the evaluation of the carcinogenic risk of chemicals to humans[M]. Switzerland: World Health Organization, 1978: 125-175.
[3] JOBB D B, HUNSINGER R, MERESZ O, et al. A study of the occurrence and inhibition of formation of N-nitrosodimethylamine(NDMA) in the Ohsweken water supply[C]. Winnipeg: Proceedings of the Fifth National Conference on Drinking Water, 1992.
[4] BARRETT S, HWANG C, GUO Y C, et al. Occurrence of NDMA in drinking water: North American survey, 2001-2003[C]. Anaheim: Proceedings of American Water Works Association Annual Conference and Exhibition, 2003.
[5] CHARROIS J W A, BOYD J M, FROESE K L, et al. Occurrence of N-nitrosamines in Alberta public drinking-water distribution systems[J]. Journal of Environmental Engineering & Science, 2007, 6(1): 103-114.
[6] ASAMI M, OYA M, KOSAKA K. A nationwide survey of NDMA in raw and drinking water in Japan[J]. Science of the Total Environment, 2009(11): 3540-3545.
[7] BEI E, SHU Y Y, LI S X, et al. Occurrence of nitrosamines and their precursors in drinking water systems around mainland China[J]. Water Research, 2016, 50(13):168-175.
[8] MEHMET A, SEVKET A. Simultaneous determination of aliphatic and aromatic amines in water and sediment samples by ion-pair extraction and gas chromatography-mass spectrometry[J]. Journal of Chromatography A, 2006(1): 88-94.
[9] PLANAS C, PALACIOS O, VENTURA F, et al. Analysis of nitrosamines in water by automated SPE and isotope dilution GC/HRMS-Occurrence in the different steps of a drinking water treatment plant, and in chlorinated samples from a reservoir and a sewage treatment plant effluent[J]. Talanta, 2008, 76(4): 906-913.
[10] KRAUSS M, HOLLENDER J. Analysis of nitrosamines in wastewater: Exploring the trace level quantification capabilities of a hybrid linear ion trap/orbitrap massspectrometer[J]. Analytical Chemistry, 2008, 80(3): 834-842.
[11] MUNCH J W, BESSETT M V. Method 521: Determination of nitrosamines in drinking water by solid phase extraction and capillary column gas chromatography with large volume injection and chemical ionization tandem mass spectrometry (MS/MS)[Z]. 2004.
[12] ZHAO Y Y, BOYD J, HRUDEY S E, et al. Characterization of new nitrosamines in drinking water using liquid chromatography tandem mass spectrometry[J]. Environmental Science & Technology, 2006, 40 (24): 7636-7641.
[13] WANG W F, HU J Y, YU J W, et al. Determination of N-nitrosodimethylamine in drinking water by UPLC-MS/MS[J]. Journal of Environmental Sciences, 2010, 22(10): 1508-1512.
[14] PLUMLEE M H, LOPEZ-MESAS M, HEIDLBERGER A, et al. N-nitrosodimethylamine (NDMA) removal by reverse osmosis and UV treatment and analysis via LC-MS/MS[J]. Water Research, 2008, 42 (1-2): 347-355.Simultaneous Determination of Multiple Nitrosamines in Drinking Water by UPLC/MS-MS