水杨酸对孕穗期低温胁迫寒地粳稻颖花形成的影响
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摘要
为探讨外源水杨酸(Salicylic acid,SA)对孕穗期低温胁迫寒地粳稻颖花形成的影响,丰富寒地粳稻化控抗寒研究,试验以耐冷品种东农428和冷敏感品种松粳10号为供试材料,设置5个SA浓度(0.25、0.50、1.00、2.00和4.00 mmol·L~(-1)),设置低温清水对照(Cold)与常温清水对照(CK),分析外源SA对孕穗期低温胁迫寒地粳稻颖花形成、干物质积累及产量的影响。结果表明,与CK处理相比,低温胁迫下水稻产量及产量构成指标显著降低。施用不同浓度SA提高寒地粳稻花粉育性、颖花分化数、籽粒干物质积累量、灌浆速率、产量及产量构成指标,随喷施SA浓度增加,东农428与松粳10号花粉育性、颖花分化数、籽粒干物质积累量、灌浆速率、产量及产量构成指标均表现为先升后降趋势。分析得出,孕穗期低温胁迫下东农428与松粳10号喷施外源SA最适浓度为0.50 mmol·L~(-1)。
In order to investigate the effect of salicylic acid(SA)on the formation of spikelet in japonica rice under low-temperature stress at booting stage and enrich the physiological basis of chemical control research, using the cold-tolerant variety Dongnong 428 and the cold-sensitive variety Songjing 10 as experimental materials, and five SA concentrations(0.25, 0.50, 1.00, 2.00, and 4.00 mmol?L~(-1)) were set up. The effect of exogenous SA on spikelet formation, dry matter amount, and yield of japonica rice were analyzed under low-temperature stress at booting stage with low temperature water control(Cold) and normal water control(CK). The results showed that compared with the normal water control, the yield and the yield components significantly reduced. Application different concentrations of SA could significantly improve pollen fertility, spikelet form, dry matter amount,grouting rate, yield and yield components of japonica rice. With the increase of SA concentration, the pollen fertility of Dongnong 428 and Songjing 10, and the spikelet form, the dry matter amount, the grouting rate, the yield and yield components all showed a trend of increasing firstly and then decreasing. According to the analysis, the optimum concentration of SA in Dongnong 428 and Songjing10 sprayed by low-temperature stress at booting stage was 0.50 mmol?L~(-1).
In order to investigate the effect of salicylic acid(SA)on the formation of spikelet in japonica rice under low-temperature stress at booting stage and enrich the physiological basis of chemical control research, using the cold-tolerant variety Dongnong 428 and the cold-sensitive variety Songjing 10 as experimental materials, and five SA concentrations(0.25, 0.50, 1.00, 2.00, and 4.00 mmol?L~(-1)) were set up. The effect of exogenous SA on spikelet formation, dry matter amount, and yield of japonica rice were analyzed under low-temperature stress at booting stage with low temperature water control(Cold) and normal water control(CK). The results showed that compared with the normal water control, the yield and the yield components significantly reduced. Application different concentrations of SA could significantly improve pollen fertility, spikelet form, dry matter amount,grouting rate, yield and yield components of japonica rice. With the increase of SA concentration, the pollen fertility of Dongnong 428 and Songjing 10, and the spikelet form, the dry matter amount, the grouting rate, the yield and yield components all showed a trend of increasing firstly and then decreasing. According to the analysis, the optimum concentration of SA in Dongnong 428 and Songjing10 sprayed by low-temperature stress at booting stage was 0.50 mmol?L~(-1).
引文
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[14]贾琰,沈阳,邹德堂,等.孕穗期冷水灌溉对寒地粳稻籽粒灌浆及其氮素积累的影响[J].中国水稻科学,2015,29(3):259-272.
[15]赵宏伟,张妍,贾琰,等.分蘖期低温胁迫下施用外源物质对寒地粳稻生长发育及产量形成的影响[J].东北农业大学学报,2018,49(8):10-23.
[16]Zeng L,Shannon M C.Effects of salinity on grain yield and yield components of rice at different seeding densities[J].Agronomy Journal,2000,92:418-423.
[17]Siband P,Wey J,Oliver R,et al.Analysis of the yield of two groups of tropical maize cultivars.varietal characteristics,yield potentials,optimum densities[J].Agronomie,1999,19(5):379-394.
[18]Rajjou L,Belghazi M,Huguet R,et al.Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms[J].Plant Physiology,2006,141(3):910-923.
[19]Xie Z,Zhang Z L,Hanzlik S,et al.Salicylic acid inhibits gibberellin-induced alpha-amylase expression and seed germination via a pathway involving an abscisic-acid-inducible WRKY gene[J].Plant Molecular Biology,2007,64(3):293-303.
[2]赵秀琴,张婷,王文生,等.水稻低温胁迫不同时间的代谢物谱图分析[J].作物学报,2013,39(4):720-726.
[3]程式华,李建.现代中国水稻[M].北京:金盾出版社,2007.
[4]张矢,徐一戎.寒地稻作[M].等.哈尔滨:黑龙江科学技术出版社,1990.
[5]吕俊,张蕊,宗学凤,等.水杨酸对高温胁迫下水稻幼苗抗热性的影响[J].中国生态农业学报,2009,17(6):1168-1171.
[6]Mishra A,Choudhuri M A.Effects of salicylic acid on heavy metal-induced membrane deterioration mediated by lipoxygenase in rice[J].Biologia Plantarum(Prague),1999,42(3):409-415.
[7]马培芳,李利红,杨亚军,等.水杨酸对高温强光胁迫下小麦叶绿体D_1蛋白磷酸化及光系统Ⅱ功能的影响[J].应用生态学报,2008,19(12):2632-2636.
[8]松岛省三.稻作的理论与技术[M].北京:中国农业出版社,1979.
[9]张荣萍,马均,蔡光泽,等.开花期低温胁迫对四川攀西稻区水稻开花结实的影响[J].作物学报,2012,38(9):1734-1742.
[10]娄伟平,孙永飞,吴利红,等.孕穗期气象条件对水稻每穗总粒数和结实率的影响[J].中国农业气象,2007(3):296-299.
[11]郭萌生,张红林,谢勇,等.温度条件对杂交中晚稻结实率的影响[J].中国农业气象,2008(3):304-307,319.
[12]符冠富,张彩霞,杨雪芹,等.水杨酸减轻高温抑制水稻颖花分化的作用机理研究[J].中国水稻科学,2015,29(6):637-647.
[13]沙汉景,胡文成,贾琰,等.外源水杨酸、脯氨酸和γ-氨基丁酸对盐胁迫下水稻产量的影响[J].作物学报,2017,43(11):1677-1688.
[14]贾琰,沈阳,邹德堂,等.孕穗期冷水灌溉对寒地粳稻籽粒灌浆及其氮素积累的影响[J].中国水稻科学,2015,29(3):259-272.
[15]赵宏伟,张妍,贾琰,等.分蘖期低温胁迫下施用外源物质对寒地粳稻生长发育及产量形成的影响[J].东北农业大学学报,2018,49(8):10-23.
[16]Zeng L,Shannon M C.Effects of salinity on grain yield and yield components of rice at different seeding densities[J].Agronomy Journal,2000,92:418-423.
[17]Siband P,Wey J,Oliver R,et al.Analysis of the yield of two groups of tropical maize cultivars.varietal characteristics,yield potentials,optimum densities[J].Agronomie,1999,19(5):379-394.
[18]Rajjou L,Belghazi M,Huguet R,et al.Proteomic investigation of the effect of salicylic acid on Arabidopsis seed germination and establishment of early defense mechanisms[J].Plant Physiology,2006,141(3):910-923.
[19]Xie Z,Zhang Z L,Hanzlik S,et al.Salicylic acid inhibits gibberellin-induced alpha-amylase expression and seed germination via a pathway involving an abscisic-acid-inducible WRKY gene[J].Plant Molecular Biology,2007,64(3):293-303.