摘要
研究不同大孔吸附树脂对薰衣草中迷迭香酸静态吸附与解吸特性,筛选出一种吸附及解吸效果良好的树脂LX-60,其静态吸附量与解吸量分别达到5.12 mg/g和4.86 mg/g。对LX-60进行吸附和解析条件考察,最终得到纯度为15.6%的迷迭香酸产品,其得率为86.79%。比纯化前的迷迭香酸质量浓度0.37%提高了42倍。本研究表明,利用大孔吸附树脂能够纯化薰衣草中的迷迭香酸,为通过薰衣草提取分离来生产迷迭香酸提供理论和实验基础。
Static adsorption and desorption of characteristics of different macroporous adsorption resin was studied,LX-60 has a good effect to adsorb and desorb. The static adsorption and desorption of quantity was 5.12 mg/g and 4.86 mg/g. And adsorption and desorption of condition was investigated for LX-60,products of rosemary acid was got in the end,purity was 15.6% and yield was 86.79%. The purity of rosemary acid was 42 times higher than the purity of 0.37%. This study indicated it was feasible to purify rosemary acid in the lavender,as well as this research provided theory and experimental basis for production.
引文
[1]PETERSEN M,SIMMONDS M S J.Molecules of interest-rosmarinic acid[J].Phytochemistry,2003,62(2):121-125.
[2]YUAN Ganjun,LONG Lina.Separation of rosmarinic acid from rosmarinus officinalis L with macroporous absorption resin[J].Lishizhen Medicineand Materia Medica Research,2010,21(1):234-236.(in Chinese)
[3]OU Yuhang,LI Jie,CHEN Qiang.Study on separation and enrichment of rosmarinic acid from sarcandr glabra extract by macroporous resin[J].China Medical Herald,2012,9(36):128-131.(in Chinese)
[4]FONTELES,ANALU A,de Souza,et al.Rosmarinic acid prevents against memory deficits inischemic mice[J].Behavioural Brain Research,2016,297:91-103.
[5]FRANKEL E N,HUANG S W,AESCHBACH R,et al.Antioxidant activity of a rosemary extract and its constituents,carnosic acid,carnosol,and rosmarinicacid,in bulk oil and oil-in-wateremulsion[J].Journal of Agricultural and Food Chemistry,1996,44(1):131-135.
[6]OSAKABE N,YASUDA A,NATSUME M,et al.Rosmarinic acid inhibits epidermal inflammatory responses:anticarcinogenic effect of Perilla frutescens extract in the murine two-stage skin model[J].Carcinogenesis,2004,25(4):549-557.
[7]OSAKABE N,TAKANO H,SANBONGI C,et al.Anti-inflammatory and anti-allergic effect of rosmarinic acid(RA);inhibition of seasonal allergic rhinoconjunctivitis(SAR)and its mechanism[J].Biofactors,2004,21(1):127-131.
[8]LI Chenyang,ZHAO Jun,XU Fang,et al.Determination of the content of rosmarinic acid in Lavandula angustifolia[J].Northwest Pharm,2014,29(3):230-232.(in Chinese)
[9]UENO T,MATSUI Y,MASUDA H,et al.Antidepressant-like effects of 3-(3,4-dihydroxyphenyl)lactic acid isolated from lavender(Lavandula angustifolia)flowers in mice[J].Food Science and Technology Research,2014,20(6):1213-1219.
[10]PENG Qing,LI Xiaogang,LIU Yaming.Macroporous adsorption resin is reviewed[J].Journal of Practical Traditional Chinese Medicine,2013,29(5):409-412.(in Chinese)
[11]SHI Lei,TIAN Yumiao,YU Wenguo,et al.Separation and purification of 3-methylxanthine from crystallization mother liquor with macroporous adsorption resin[J].Chinese Journal of Pharmaceuticals,2015,46(12):1347-1351.(in Chinese)
[12]FU B Q,LIU J,LI H,et al.The application of macroporous resins in the separation of licorice flavonoids and glycyrrhizic acid[J].Journal of Chromatography A,2005,1089(1):18-24.
[13]LI Yingchang,ZHENG Fenge,MENG Xianjun.Studies on purification of anthocyanins from blueberry fruits by macroporous resins[J].Journal of Food Science and Biotechnology,2009,28(4):496-500.(in Chinese)
[14]QIAN Zhu,XU Peng,ZHANG Kechang,et al.Extraction of ganoderic triterpenes from fermented broth by macroporous resin[J].Journal of Food Science and Biotechnology,2006,25(6):111-114.(in Chinese)
[15]ZHOU Ping,LU Xiaoling,HU Chungang.Study on separation and purification of rosmarinic acid[J].Ion Exchange and Adsorption,2011,27(4):304-314.(in Chinese)
[16]BAI Duolong,YANG Kaihua.Macroporous adsorption resin purification technology and application[J].Strait Pharmaceutical Journal,2007,19(9):96-99.(in Chinese)
[17]SUN Xing,SHENG Huagang,ZHANG Chao,et al.Influence of chitosan flocculation sedimentation method on flavonoids and alkaloids from gegen Qinlian decoction[J].Chinese Journal of Experimental Traditional Medical Formulae,2012,18(20):12-15.(in Chinese)