连云港山楂叶黄酮的超声提取及体外活性研究
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摘要
以连云港花果山山楂叶为原料,以总黄酮提取率为衡量提取工艺的指标。通过单因素和正交实验设计优化了超声辅助提取山楂叶黄酮的工艺,确定了从山楂叶中提取黄酮的最佳工艺条件:乙醇浓度70%,液料比20:1 m L/g,超声温度70℃,超声时间40 min,提取3次,山楂叶黄酮提取率为2.53%。此外,采用琼脂平板扩散抑菌法和DPPH·法研究了连云港山楂叶黄酮对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌的抑制作用以及抗氧化效应,结果表明山楂叶黄酮具有明显抑菌活性和清除DPPH·的能力。
Hawthorn leaves as raw material were collected from Huaguo mountain of Lianyungang city and the yield of total flavonoids was used as evaluation indicator. The extraction technology of flavonoids from hawthorn leaves with ultrasonic assisted method was optimized by single and orthogonal experimental design, and the optimal extraction technology was obtained as following: ethanol concentration of 70%, solvent-to-liquid ratio of 20:1(m L/g), extraction temperature of 70℃, extraction time of 40 min and extraction times of 3. The yield of flavonoids from hawthorn leaves is 2.53%. Moreover, the inhibitory effect of the flavonoids against Staphylococcus aureus, Escherichia coli and Bacillus subtilis was determined by the agar diffusion method, and the antioxidant effect was evaluated by DPPH method. The results showed that the flavonoids extracted from hawthorn leaves had good antibacterial activity and excellent scavenging capacity to DPPH radical.
Hawthorn leaves as raw material were collected from Huaguo mountain of Lianyungang city and the yield of total flavonoids was used as evaluation indicator. The extraction technology of flavonoids from hawthorn leaves with ultrasonic assisted method was optimized by single and orthogonal experimental design, and the optimal extraction technology was obtained as following: ethanol concentration of 70%, solvent-to-liquid ratio of 20:1(m L/g), extraction temperature of 70℃, extraction time of 40 min and extraction times of 3. The yield of flavonoids from hawthorn leaves is 2.53%. Moreover, the inhibitory effect of the flavonoids against Staphylococcus aureus, Escherichia coli and Bacillus subtilis was determined by the agar diffusion method, and the antioxidant effect was evaluated by DPPH method. The results showed that the flavonoids extracted from hawthorn leaves had good antibacterial activity and excellent scavenging capacity to DPPH radical.
引文
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[2]英锡相,李绍维,林延会,等.山楂叶植物学及化学成分研究近况[J].辽宁中医学院学报,2001,3(2):98-99.
[3]Li H L,Song F R,Xing J P,et a1.Screening and structural characterization ofα-glucosidase inhibitors from hawthorn leaf flavonoids extract by ultrafiltration LC-DAD-MSn and SORI-CID FTICR MS[J].J Am Soc Mass Spectrom,2009,20(8):1496-1503.
[4]李冬菊,林阳.山楂叶黄酮的提取及其鉴定[J].辽宁中医杂志,2003,30(7):578-579.
[5]Tadi V M,Dobri S,Markovi G.M,et a1.Antiinflammatory,gstroprotective,fee-rdical-savenging,and atimicrobial ativities of hwthorn brries ehanol etract[J].Journal of Agricultural and Food Chemisty,2008,56(17):7700-7709.
[6]许瑞波,刘炜玮,马卫兴,等.山楂叶多糖的提取工艺研究[J].食品科技,2008,10:149-151.
[7]石瑞平,周庆英,陈兴菊.不同生长期山楂叶黄酮类有效成分的含量测定[J].中国药业,2002,11(7):55.
[8]秦猛,韩春英.山楂叶提取物的研究进展[J].齐鲁药事,2009,28(9):542-545.
[9]王晓华,张晓丹.山楂叶总黄酮药理作用及机制研究进展[J].内蒙古民族大学学报:自然科学版,2010,25(4):436-438.
[10]王建全,张亚娜,林敏.乙醇/硫酸铵双水相体系分离纯化款冬花总黄酮[J].井冈山大学学报:自然科学版,2016,37(1):40-45.
[11]许瑞波,周洪英,陈林,等.地瓜叶总黄酮的超声辅助提取及生物活性研究[J].食品科学,2013,34(14):141-146.
[12]勾明明,刘梁,张春枝.采用DPPH法测定26种植物的抗氧化活性[J].食品与发酵工业,2010,36(3):148-150.
[13]石雪萍,李小华,杨爱萍.南京雨花茶中总黄酮提取以及DPPH自由基清除活性研究[J].江苏农业科学,2013,41(6):238-240.