超声提取山楂叶中多糖工艺优化及抗氧化性研究
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摘要
目的筛选山楂叶多糖的最佳提取条件及抗氧化性研究。方法采用乙醇溶液做提取试剂,超声辅助提取山楂叶多糖,通过单因素、正交试验确定最优提取条件,通过Sevage法除去其中蛋白质,用1,1-二苯基-2-三硝基苯肼自由基(DPPH·)法测定提取物即多糖的自由基清除率。结果最佳提取条件为料液比为1∶20,60℃提取20 min,提取2次;山楂叶多糖对1,1-二苯基-2-三硝基苯肼自由基清除率达到65.1%。结论超声辅助可以较好提取山楂叶多糖,所提取的山楂叶多糖具有良好的抗氧化活性。
Objective To screen the optimum extraction conditions and antioxidative activity of polysaccharide from hawthorn leaves.Methods Ethanol extract was used as an extraction reagent and ultrasonic assisted extraction of polysaccharides from hawthorn leaves. The optimal extraction conditions were determined by single factor,orthogonal test,and protein was removed by Sevage method.The radical scavenging rate of polysaccharides was determined by DPPH·.Results The optimum extraction conditions were as follows: the ratio of material to liquid is 1 ∶ 20,extracted at 60 ℃ for 20 min,and 2 times were extracted.The scavenging rate of hawthorn leaf polysaccharide on DPPH· was 65.1%. Conclusion The polysaccharide of hawthorn leaves can be extracted with the aid of ultrasound,and the extracted polysaccharides from hawthorn leaves have good antioxidant activity.
Objective To screen the optimum extraction conditions and antioxidative activity of polysaccharide from hawthorn leaves.Methods Ethanol extract was used as an extraction reagent and ultrasonic assisted extraction of polysaccharides from hawthorn leaves. The optimal extraction conditions were determined by single factor,orthogonal test,and protein was removed by Sevage method.The radical scavenging rate of polysaccharides was determined by DPPH·.Results The optimum extraction conditions were as follows: the ratio of material to liquid is 1 ∶ 20,extracted at 60 ℃ for 20 min,and 2 times were extracted.The scavenging rate of hawthorn leaf polysaccharide on DPPH· was 65.1%. Conclusion The polysaccharide of hawthorn leaves can be extracted with the aid of ultrasound,and the extracted polysaccharides from hawthorn leaves have good antioxidant activity.
引文
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[2]张燕,张树淼,王飞,等.近年来植物多糖提取方法研究进展[J].农产品加工,2015(6):65-68.
[3]黄飞,梁智群.对山楂叶中多糖活性成分的分离提取研究[J].广西轻工业,2006(6):45-46.
[4]张振华,解宁湘,王基云,等.DPPH法测定沙栆花水提物各组分清除自由基的研究[J].时珍国医国药,2011,32(11):2703-2704.
[5]周云美,李毅群,陈填烽,等.光谱法研究晒代大蒜新素与大蒜新素对DPPH自由基的清除作用[J].食品科学,2013,34(3):7-10.
[6]张越锋,李福燕,吴瑛.红枣多糖及红枣硒多糖抗氧化性活性的比较研究[J].食品研究与开发,2015,36(3):4-9.
[7]WU H C,CHEN H M,SHIAU C Y.Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel[J].Food Res Int,2003,36(9-10):949-957.
[8]WU F,YAN H,MA X,et al.Structural characterization and antioxidant activity of purified poiysaccharide from cultured Cordyceps militaris[J].Afr J Microbiol Res,2011,5(18):2743-2751.
[9]刘钧发,冯梦莹,游丽君,等.超声和水提法提取灵芝多糖的结构和抗氧化性的比较研究[J].食品工业科学,2013,15(24):88-92.
[10]滕海英,祝国强,黄平,等.正交试验设计实例分析[J].药学服务与研究,2008,8(1):75-77.