摘要
目的观察尖叶假龙胆对脂多糖(LPS)诱导脓毒症大鼠急性肾损伤的保护作用。方法通过单次腹腔注射LPS(7 mg/kg)制备模型,以地塞米松为阳性对照药,通过观察与检测大鼠的一般情况、尿量、血清尿素氮(BUN)和肌酐(Scr)及尿中肾组织肾损伤分子-1(KIM-1)、中性粒细胞明胶酶相关脂质运载蛋白(NGAL)水平,考察尖叶假龙胆对大鼠急性肾损伤保护作用。结果造模后24 h时,尖叶假龙胆组、地塞米松组血清Scr和BUN、尿液中KIM-1与NGAL含量与模型组差异有统计学意义(P <0.05)。结论尖叶假龙胆对LPS诱导脓毒症模型大鼠急性肾损伤模型有一定的保护作用,其机制可能与抑制KIM-1与NGAL蛋白有关。
Objective: To investigate the protective effect of Gentianella Acuta on LPS-induced acute kidney injury in sepsis rats. Methods: The model was established by single intraperitoneal injection of LPS(7 mg/kg).Dexamethasone was used as positive control drug. The protective effects of Gentianella acuta on acute renal injury in rats were studied by observing and detecting the general condition,urine volume,BUN,Scr,KIM-1 and NGAL levels in urine. Results: 24 h after model establishment,serum Scr,BUN and levels of urine KIM-1 and NGAL of other groups were significantly different from those in the model group(P < 0.05). Conclusion: Gentianella acuta has a protective effect on LPS-induced acute renal injury in rats,which may be related to the inhibition of KIM-1 and NGAL protein.
引文
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