一测多评法测定香鳞毛蕨有效部位中10种间苯三酚类成分
|
摘要
目的建立香鳞毛蕨DryopterisFragrans有效部位中10种间苯三酚类成分的一测多评测定方法。方法采用高效液相色谱法,以绵马素BB为内参物,测定其与绵马素PB、绵马素AB、黄绵马酸BB、田基黄绵马酸A、黄绵马酸PB、异黄绵马酸PB、黄绵马酸AB、Compound VI和绵马酚B的相对校正因子,采用相对校正因子计算这9种间苯三酚成分的量,实现一测多评。同时采用外标法测定有效部位中该10种成分的量,并比较2种测定方法的差异,以验证一测多评法的可行性和准确性。结果各相对校正因子重复性良好,12批有效部位中10种间苯三酚成分量的计算值与实测值无显著性差异。结论在缺少对照品的情况下,以绵马素BB为内参物,建立的一测多评法可用于香鳞毛蕨有效部位的定量分析,为香鳞毛蕨多指标成分质量评价提供参考。
Objective To establish quantitative analysis of multi-components with single marker(QAMS) for determination of 10 phloroglucinol contents in effective fraction of Dryopteris fragrans. Methods The relative correction factors of nine phloroglucinol(aspidin PB, aspidin AB, flavaspidic acid BB, saroaspidin A, flavaspidic acid PB, disflavapidic acid PB, flavaspidic acid AB, compound VI, and aspidinol B) were determined by HPLC method with the aspidinn BB as the internal standard, which were to calculate the content of each. At the same time, external standard method(ESM) was used to determine the contents of 10 components in effective fraction, and the differences between the two methods were compared to verify the feasibility and accuracy of QAMS method. Results The relative correction factor(RCF) was good. There was no significant difference between the quantitative results with the two methods in the 12 batches of 10 phloroglucinols. Conclusion The established QAMS method can be used for quantitative analysis of D. fragrans with aspidinn BB as the internal standard in the absence of reference substances, and provided a reference for the multi-index quality evaluation in effective fraction of D. fragrans.
Objective To establish quantitative analysis of multi-components with single marker(QAMS) for determination of 10 phloroglucinol contents in effective fraction of Dryopteris fragrans. Methods The relative correction factors of nine phloroglucinol(aspidin PB, aspidin AB, flavaspidic acid BB, saroaspidin A, flavaspidic acid PB, disflavapidic acid PB, flavaspidic acid AB, compound VI, and aspidinol B) were determined by HPLC method with the aspidinn BB as the internal standard, which were to calculate the content of each. At the same time, external standard method(ESM) was used to determine the contents of 10 components in effective fraction, and the differences between the two methods were compared to verify the feasibility and accuracy of QAMS method. Results The relative correction factor(RCF) was good. There was no significant difference between the quantitative results with the two methods in the 12 batches of 10 phloroglucinols. Conclusion The established QAMS method can be used for quantitative analysis of D. fragrans with aspidinn BB as the internal standard in the absence of reference substances, and provided a reference for the multi-index quality evaluation in effective fraction of D. fragrans.
引文
[1]朱冲冲,彭冰,曾祖平,等.香鳞毛蕨的化学成分及药理作用研究进展[J].中国药房,2017,28(10):1418-1423.
[2]Li N,Gao C,Peng X,et al.Aspidin BB,a phloroglucinol derivative,exerts its antibacterial activity against Staphylococcus aureus by inducing the generation of reactive oxygen species[J].Res Microbiol,2014,165(4):263-272.
[3]黄奕曦,沈志滨,江涛,等.香鳞毛蕨有效部位对红色毛癣菌体外抗真菌的作用[J].广东药科大学学报,2016,32(1):78-82.
[4]Sun Y,Mu F,Li C,et al.Aspidin BB,a phloroglucinol derivative,induces cell cycle arrest and apoptosis in human ovarian HO-8910 cells[J].Chemico-Biol Inter,2013,204(2):88-97.
[5]Wan D,Jiang C,Hua X,et al.Cell cycle arrest and apoptosis induced by aspidin PB through the p53/p21 and mitochondria-dependent pathways in human osteosarcoma cells[J].Anti Cancer Drugs,2015,26(9):931-941.
[6]Lee S M,Na M R,Min B S,et al.Antioxidant activity of two phloroglucinol derivatives from Dryopteris crassirhizoma[J].Biol Pharm Bull,2003,26(9):1354-1356.
[7]王洁,唐春萍,江涛,等.香鳞毛蕨有效部位对糠秕马拉色菌的体外抗真菌作用研究[J].中成药,2015,37(3):642-645.
[8]杜文钊,宋国强,刘海燕,等.香鳞毛蕨中间苯三酚类化合物的研究[J].广东药科大学学报,2016,32(1):22-24.
[9]陈文浩,宋国强,贾小舟,等.香鳞毛蕨中1对间苯三酚类同分异构体的分离与抗真菌活性研究[J].中草药,2017,48(3):433-436.
[10]刘海燕,杜文钊,宋国强,等.香鳞毛蕨药材的质量标准研究[J].广东药学院学报,2016,32(1):36-40.
[11]田莉,施洋,畅静,等.新疆香鳞毛蕨药材质量研究[J].新疆医科大学学报,2014,37(9):1111-1114.
[2]Li N,Gao C,Peng X,et al.Aspidin BB,a phloroglucinol derivative,exerts its antibacterial activity against Staphylococcus aureus by inducing the generation of reactive oxygen species[J].Res Microbiol,2014,165(4):263-272.
[3]黄奕曦,沈志滨,江涛,等.香鳞毛蕨有效部位对红色毛癣菌体外抗真菌的作用[J].广东药科大学学报,2016,32(1):78-82.
[4]Sun Y,Mu F,Li C,et al.Aspidin BB,a phloroglucinol derivative,induces cell cycle arrest and apoptosis in human ovarian HO-8910 cells[J].Chemico-Biol Inter,2013,204(2):88-97.
[5]Wan D,Jiang C,Hua X,et al.Cell cycle arrest and apoptosis induced by aspidin PB through the p53/p21 and mitochondria-dependent pathways in human osteosarcoma cells[J].Anti Cancer Drugs,2015,26(9):931-941.
[6]Lee S M,Na M R,Min B S,et al.Antioxidant activity of two phloroglucinol derivatives from Dryopteris crassirhizoma[J].Biol Pharm Bull,2003,26(9):1354-1356.
[7]王洁,唐春萍,江涛,等.香鳞毛蕨有效部位对糠秕马拉色菌的体外抗真菌作用研究[J].中成药,2015,37(3):642-645.
[8]杜文钊,宋国强,刘海燕,等.香鳞毛蕨中间苯三酚类化合物的研究[J].广东药科大学学报,2016,32(1):22-24.
[9]陈文浩,宋国强,贾小舟,等.香鳞毛蕨中1对间苯三酚类同分异构体的分离与抗真菌活性研究[J].中草药,2017,48(3):433-436.
[10]刘海燕,杜文钊,宋国强,等.香鳞毛蕨药材的质量标准研究[J].广东药学院学报,2016,32(1):36-40.
[11]田莉,施洋,畅静,等.新疆香鳞毛蕨药材质量研究[J].新疆医科大学学报,2014,37(9):1111-1114.