多花筋骨草提取物抗氧化活性研究
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摘要
目的:采用体外抗氧化实验,对多花筋骨草醇提物的抗氧化活性进行研究,为多花筋骨草的开发和应用提供参考数据。方法:用甲醇冷浸提取多花筋骨草,减压浓缩得到多花筋骨草醇提物,采用1,1-二苯基-2-三硝基苯肼(DPPH·)法、2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS+)法和总还原能力测定法,研究其提取物的抗氧化活性。结果:多花筋骨草醇提物对DPPH·、ABTS+均有清除活性,以及较强还原能力,且DPPH·清除活性(IC50值)为0.348 mg/mL、ABTS+清除活性(IC50值)为0.292 mg/mL,总还原能力(IC50值)为2.736 mg/mL。结论:多花筋骨草醇提物有较强的抗氧化活性,且抗氧化活性与醇提物质量浓度两者间存在一定量效关联。
Objective :The anti-oxidation activity of the ethanol extract of Ajuga multiflora Bunge was studied by in vitro antioxidant experiment,which provided reference data for the development and application of Ajuga multiflora Bunge. Methods :The extract of Ajuga multiflora Bunge was extracted by cold soaking with methanol,and concentrated under reduced pressure to obtain ethanol extract of Ajuga multiflora Bunge. The antioxidation activities of dried residues were detected and evaluated by three methods of DPPH·,ABTS+ and total reducing power. Results :The ethanol extract of Ajuga multifloraBunge,as the scavenging activity on DPPH·and ABTS+,and had strong reducing power,and DPPH·clearing activity(IC50 value)was 0.348 mg/mL,and ABTS+ scavenging activity(IC50 value)was 0.292 mg/mL. And total reducing power(IC50 value)was 2.736 mg/mL. Conclusion :The ethanol extract of Ajuga multiflora Bunge has strong antioxidant activity,and there is a dose-effect correlation between antioxidant activity and alcohol extract mass concentration.
Objective :The anti-oxidation activity of the ethanol extract of Ajuga multiflora Bunge was studied by in vitro antioxidant experiment,which provided reference data for the development and application of Ajuga multiflora Bunge. Methods :The extract of Ajuga multiflora Bunge was extracted by cold soaking with methanol,and concentrated under reduced pressure to obtain ethanol extract of Ajuga multiflora Bunge. The antioxidation activities of dried residues were detected and evaluated by three methods of DPPH·,ABTS+ and total reducing power. Results :The ethanol extract of Ajuga multifloraBunge,as the scavenging activity on DPPH·and ABTS+,and had strong reducing power,and DPPH·clearing activity(IC50 value)was 0.348 mg/mL,and ABTS+ scavenging activity(IC50 value)was 0.292 mg/mL. And total reducing power(IC50 value)was 2.736 mg/mL. Conclusion :The ethanol extract of Ajuga multiflora Bunge has strong antioxidant activity,and there is a dose-effect correlation between antioxidant activity and alcohol extract mass concentration.
引文
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[14]陈晓宇,田振坤,高翔,等.大孔树脂纯化五味子总木脂素及其体外还原能力研究[J].辽宁中医药大学学报,2017,19(4):39-42.
[15] Jang HJ,Lee HJ,Yoon DK,et al. Antioxidant and antimicrobial activities of fresh garlic and aged garlic by-products extracted with different solvents[J].Food Science and Biotechnology,2018,27(1):219-225.
[16]张鲁,张媛,王文全,等.甘草地上部分总黄酮提取工艺优化及抗氧化活性研究[J].辽宁中医药大学学报,2018,20(4):55-59.
[17]王雪梅,吴汉平,汪晓敏,等. 16种中草药提取物的抗氧化活性研究[J].安徽大学学报(自然科学版),2015,39(2):74-80.
[18] Arokiyaraj S,Bharanidharan R,Agastian P. Chemical composition,antioxidant activity and antibacterial mechanism of action from Marsilea minuta leaf hexane:methanol extract[J].Chemistry Central Journal,2018,12(1):105.
[19] WU NH,KE ZQ,WU S,et al. Evaluation of the antioxidant and endothelial protective effects of Lysimachia christinae Hanceextract fractions[J].BMC Complementary and Alternative Medicine,2018,18(1):128.
[20]华艳宏,庞春花,张永清,等.藜麦种子不同溶剂提取物及其抗氧化活性[J].江苏农业科学,2018,46(20):1-4.
[21]席晓志,崔晓伟,郭弘,等.响应面分析法优化后淫羊藿提取物抗氧化活性研究[J].辽宁中医药大学学报,2018,20(4):63-67.
[2]袁保刚.生地黄提取物抗氧化活性研究[D].杨陵:西北农林科技大学,2011.
[3]赵佳佳,宇佳,迟德富.多花筋骨草高频再生体系的建立[J].湖南师范大学自然科学学报,2016,39(1):30-34.
[4]熊焰,曲玮,梁敬钰.筋骨草属植物化学成分和药理作用研究进展[J].海峡药学,2012,24(2):1-7.
[5]陈银,杨凡,敖慧,等.美花圆叶筋骨草的化学成分研究[J].中草药,2017,48(17):3475-3479.
[6]张慧,夏厚林,杨孟妮,等. HPLC测定美花筋骨草中三种黄酮的含量[J].中药与临床,2017,8(2):19-21.
[7] Taleb-Senouci D,Ghomari H,Krouf D,et al. Antioxidant effect of Ajuga iva aqueous extract in streptozotocin-induced diabetic rats[J].Phytomedicine:International Journal of Phytotherapy and Phytopharmacology,2009,16(6-7):623-631.
[8] Bouderbala S,Lamri-Senhadji M,Prost J,et al. Changes in antioxidant defense status in hypercholesterolemic rats treated with Ajuga iva[J].Phytomedicine:International Journal of Phytotherapy and Phytopharmacology,2008,15(6-7):453-461.
[9] Bouderbala S,Prost J,Lacaille-Dubois MA,et al. Iridoid extracts from Ajuga iva increase the antioxidant enzyme activities in red blood cells of rats fed a cholesterol-rich diet[J].Nutrition Research,2010,30(5):358-365.
[10]李婷婷,易超凡.不同来源菊花总黄酮含量及其抗氧化活性研究[J].辽宁中医药大学学报,2015,17(5):72-74.
[11]洪佳敏,林宝妹,张朝坤,等.番石榴醇提物抗氧化能力及α-葡萄糖苷酶抑制活性研究[J].西北农林科技大学学报(自然科学版),2019,47(1):1-7.
[12] da Costa Cordeiro BMP,de Lima Santos ND,Ferreira MRA,et al. Hexane extract from Spondias tuberosa(Anacardiaceae)leaves has antioxidant activity and is an anti-Candida agent by causing mitochondrial and lysosomal damages[J].BMC Complementary and Alternative Medicine,2018,18(1):284.
[13]孙荣花,周元飞,邵莹,等.桃叶卫矛叶提取物的体外抗氧化活性及对CCL4致小鼠急性肝损伤的保护作用[J].辽宁中医药大学学报,2015,17(11):28-31.
[14]陈晓宇,田振坤,高翔,等.大孔树脂纯化五味子总木脂素及其体外还原能力研究[J].辽宁中医药大学学报,2017,19(4):39-42.
[15] Jang HJ,Lee HJ,Yoon DK,et al. Antioxidant and antimicrobial activities of fresh garlic and aged garlic by-products extracted with different solvents[J].Food Science and Biotechnology,2018,27(1):219-225.
[16]张鲁,张媛,王文全,等.甘草地上部分总黄酮提取工艺优化及抗氧化活性研究[J].辽宁中医药大学学报,2018,20(4):55-59.
[17]王雪梅,吴汉平,汪晓敏,等. 16种中草药提取物的抗氧化活性研究[J].安徽大学学报(自然科学版),2015,39(2):74-80.
[18] Arokiyaraj S,Bharanidharan R,Agastian P. Chemical composition,antioxidant activity and antibacterial mechanism of action from Marsilea minuta leaf hexane:methanol extract[J].Chemistry Central Journal,2018,12(1):105.
[19] WU NH,KE ZQ,WU S,et al. Evaluation of the antioxidant and endothelial protective effects of Lysimachia christinae Hanceextract fractions[J].BMC Complementary and Alternative Medicine,2018,18(1):128.
[20]华艳宏,庞春花,张永清,等.藜麦种子不同溶剂提取物及其抗氧化活性[J].江苏农业科学,2018,46(20):1-4.
[21]席晓志,崔晓伟,郭弘,等.响应面分析法优化后淫羊藿提取物抗氧化活性研究[J].辽宁中医药大学学报,2018,20(4):63-67.