大孔吸附树脂纯化刺梨多酚工艺的优化
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摘要
目的优化大孔吸附树脂纯化刺梨多酚工艺。方法以吸附率、解吸率为评价指标,考察4种大孔吸附树脂(ADS-17、HPD-600、AB-8、NKA-9)的吸附、解吸性能。然后,响应面法优化上样液质量浓度、pH、体积流量,以及解吸液(乙醇)体积分数、pH、体积流量。结果 AB-8型大孔吸附树脂的吸附、解吸性能最佳,最大静态吸附量、解吸率分别达8. 11 mg/g、94. 39%。最佳吸附条件为上样液质量浓度0. 7 mg/mL,pH 5. 9,体积流量2. 6 mL/min;最佳解吸条件为解吸液体积分数69%,pH 6. 3,体积流量2. 7 mL/min,多酚回收率20. 37%,含有量36. 33%,是纯化前的4. 9倍。结论该方法稳定可靠,可用于大孔吸附树脂纯化刺梨多酚工艺,也能为该类成分后续开发利用提供技术保证。
引文
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[20]程振玉,杨英杰,成乐琴,等. Box-Behnken响应面法优化组织破碎提取龙胆苦苷[J].中成药,2016,38(6):1408-1412.
[21]卢可可,郑少杰,吴素蕊,等.响应面试验优化黑脉羊肚菌多酚纯化工艺及其抗氧化活性[J].食品科学,2015,36(18):13-20.
[22]樊梓鸾.红豆越橘多酚对氧化诱导损伤及癌细胞增殖抑制作用研究[D].哈尔滨:哈尔滨工业大学,2012.
[2]陈淑珍,甄永苏.茶多酚的分子作用靶点及其在抗肿瘤药物实验治疗中的作用[J].药学学报,2013,48(1):1-7.
[3]周宁,戴旖旎,白旭光,等.姜黄素对肝癌细胞凋亡及TNF-α、IL-1β、IL-6炎性因子影响的机制研究[J].癌症进展,2017,15(11):1282-1285.
[4]李巍巍,李俊发,郭安臣,等.植物多酚神经保护作用的研究进展[J].中国卒中杂志,2015,10(2):141-146.
[5] Cabello-Hurtado F,Gicquel M,Esnault M A. Evaluation of the antioxidant potential of cauliflower(Brassica oleracea)from a glucosinolate content perspective[J]. Food Chem,2012,132(2):1003-1009.
[6] Kim Y J,Uyama H. Tyrosinase inhibitors from natural and synthetic sources:structure,inhibition mechanism and perspective for the future[J]. Cell Mol Life Sci, 2005, 62(15):1707-1723.
[7]凌关庭.有“第七类营养素”之称的多酚类物质[J].中国食品添加剂,2000(1):28-37.
[8]贵州省药品监督管理局.贵州省中药材、民族药材质量标准(2003年版)[S].贵阳:贵州科技出版社,2014.
[9] Lu X T,Bao S J. Effect of polysaccharides from Fructus Rosae Roxburghii on stress tolerance and immune function[J]. J Guangzhou Univ Tradit Chin Med,2002,19(2):141-142.
[10] Yang J T,Yang J,Yang J B,et al. Antioxidant effect of polysaccharide from Rosa roxburghii in aged mice[J]. Acta Nutr Sin,2008,30(4):407-409.
[11] Zhang C,Liu X,Qiang H,et al. Inhibitory effects of Rosa roxburghii tratt juice on in vitro oxidative modification of low density lipoprotein and on the macrophage growth and cellular cholesteryl ester accumulation induced by oxidized low density lipoprotein[J]. Clin Chim Acta,2001,313(1-2):37-43.
[12]孙红艳,胡凯中,郭志龙,等.超声波法提取刺梨多酚的工艺优化及体外抑菌活性研究[J].中国食品添加剂,2016(2):57-61.
[13]张汇慧,吴彩娥,范龚健,等.刺梨黄酮的精制及其抗氧化活性比较[J].南京林业大学学报(自然科学版),2015,39(3):101-105.
[14]何伟平,滕建文,朱晓韵,等.刺梨Vc和多酚成分稳定性及抗氧化性研究[J].广西轻工业,2011,27(9):1-3.
[15] Li W B,Sun H Y,Zhou J W,et al. Antibacterial activities,antioxidant contents and antioxidant properties of three traditional Chinese medicinal extracts[J]. Bangladesh J Pharmacol,2015,10:131-137.
[16]刘清,李虎虎,姚惠源,等.大孔吸附树脂分离富集大麦多酚的研究[J].食品科学,2007,28(11):98-102.
[17]李春美,徐淑芬,党美珠,等.芒果多酚的提取纯化工艺研究[J].食品科技,2010,35(5):214-218.
[18] Chen Q Q,Gan Z L,Zhao J H,et al. In vitro comparison of antioxidant capacity of cumin(Cuminum cyminum L.)oils and their main components[J]. LWT-Food Sci Technol,2014,55(2):632-637.
[19]马俊鹏,马迪,安晓晶,等.响应面法优化天山花楸总黄酮回流提取工艺[J].中成药,2017,39(3):509-512.
[20]程振玉,杨英杰,成乐琴,等. Box-Behnken响应面法优化组织破碎提取龙胆苦苷[J].中成药,2016,38(6):1408-1412.
[21]卢可可,郑少杰,吴素蕊,等.响应面试验优化黑脉羊肚菌多酚纯化工艺及其抗氧化活性[J].食品科学,2015,36(18):13-20.
[22]樊梓鸾.红豆越橘多酚对氧化诱导损伤及癌细胞增殖抑制作用研究[D].哈尔滨:哈尔滨工业大学,2012.