铁皮石斛叶总黄酮泡腾颗粒剂的制备与抗氧化活性研究
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摘要
目的获得铁皮石斛叶黄酮提取物泡腾颗粒剂的制作工艺并测定其抗氧化能力。方法采取L_9(3~4)正交设计法,以粒度、溶化时间为评价指标,考察乳糖的用量、柠檬酸与碳酸氢钠的配比、聚乙二醇6000(PEG6000)的用量对泡腾颗粒剂影响,以最佳处方制备泡腾颗粒剂,并采用《中国药典》2015年版方法对其进行质量评价。采用亚硝酸钠比色法测定制剂中总黄酮的含量。采用1,1-二苯基-2-三硝基苯肼(DPPH)法测定制剂的抗氧化能力。结果最佳处方为乳糖用量40%,柠檬酸与碳酸氢钠的配比1.3∶1,聚乙二醇6000用量9%。所得制剂符合《中国药典》2015年版相关标准,其总黄酮含量为(1.29±0.08)mg·g~(-1),1,1-二苯基-2-三硝基苯肼清除能力的IC_(50)为(3.77±0.10)mg·mL~(-1)。结论本论文将铁皮石斛叶黄酮提取物制备成吸收快、生物利用度高、便于运输携带的泡腾颗粒制剂,该制剂黄酮含量丰富,具有较好的抗氧化能力。
Objective To prepare effervescent granules from Dendrobium candidum leaves and analyze its total flavonoids contents and antioxidant activity.Methods With the particle size,dissolvability time as the evaluation index,the amount of lactose,the ratio of citric acid to sodium bicarbonate and the dosage of PEG6000 which impacted response values significantly were investigated by L_9(3~4) orthogonal design method to find the best of the formula.The obtained formulation was evaluated according to Chinese Pharmacopoeia(2015 edition).Its total flavonoids contents were detected by NaNO_(2 ) photo colorimetric method.Its antioxidant activity was detected by DPPH method.Results The formula of effervescent granules was established as follows:the percentage of lactose and PEG6000 were 40% and 9% respectively;The ratio of citric acid to sodium bicarbonate was 1.3∶1.The obtained effervescent granules met the standard of the Chinese Pharmacopoeia.The content of total flavonoids was(1.29±0.08)mg·g~(-1),and it exerted desirable antioxidant activity with IC_(50) as(3.77±0.10)mg·mL~(-1).Conclusion In this experiment,the total flavonoids extract from Dendrobium candidum leaves was made into effervescent granules,which could be absorbed quickly,exerted high bioavailability and could be used conveniently.This formulation contained high number of flavonoids and had good antioxidant activity.
Objective To prepare effervescent granules from Dendrobium candidum leaves and analyze its total flavonoids contents and antioxidant activity.Methods With the particle size,dissolvability time as the evaluation index,the amount of lactose,the ratio of citric acid to sodium bicarbonate and the dosage of PEG6000 which impacted response values significantly were investigated by L_9(3~4) orthogonal design method to find the best of the formula.The obtained formulation was evaluated according to Chinese Pharmacopoeia(2015 edition).Its total flavonoids contents were detected by NaNO_(2 ) photo colorimetric method.Its antioxidant activity was detected by DPPH method.Results The formula of effervescent granules was established as follows:the percentage of lactose and PEG6000 were 40% and 9% respectively;The ratio of citric acid to sodium bicarbonate was 1.3∶1.The obtained effervescent granules met the standard of the Chinese Pharmacopoeia.The content of total flavonoids was(1.29±0.08)mg·g~(-1),and it exerted desirable antioxidant activity with IC_(50) as(3.77±0.10)mg·mL~(-1).Conclusion In this experiment,the total flavonoids extract from Dendrobium candidum leaves was made into effervescent granules,which could be absorbed quickly,exerted high bioavailability and could be used conveniently.This formulation contained high number of flavonoids and had good antioxidant activity.
引文
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[12] 韦献雅,殷丽琴,钟成,等.DPPH法评价抗氧化活性研究进展[J].食品科学,2014,35(9):317-322.
[13] 成兰英,梁书凤,张治强.DPPH法研究麦冬提取物抗氧化活性[J].精细化工,2012,29(9):870-874.
[2] 周桂芬,吕圭源.铁皮石斛叶中黄酮碳苷类成分的高效液相指纹图谱及指标成分的含量测定[J].中国药学杂志,2012,47(11):889-893.
[3] 唐丽,李菁,龙华,等.不同生长龄铁皮石斛茎与叶中总多糖、总生物碱及总黄酮含量的差异[J].广东农业科学,2015(8):17-21.
[4] 周桂芬,吕圭源.铁皮石斛不同部位黄酮碳苷类成分及清除DPPH自由基能力比较研究[J].中国中药杂志,2012,37(11):1536-1540.
[5] 杨群,张锴.石榴皮泡腾颗粒剂的研制[J].安徽农业科学,2012,40(15):8447.
[6] 龚庆芳,何金祥,黄宁珍,等.铁皮石斛花化学成分及抗氧化活性研究[J].食品科技,2014,39(12):106-110.
[7] 林为艺,颜美秋,吕圭源,等.铁皮石斛茎非多糖与粗多糖体内外抗氧化活性的比较[J].中药药理与临床,2016,32(2):138-141.
[8] LAM Y,NG T B,YAO R M,et al.Evaluation of chemical constituents and important mechanism of pharmacological biology in dendrobium plants[J].Evid Based Complement Alternat Med,2015(2015):841752.
[9] 国家药典委员会.中华人民共和国药典2015年版(四部)[S].北京:中国医药科技出版社,2015:7-8.
[10] 曹苗苗,徐桂红,刘洪波,等.响应面法优化降脂胶囊干法制粒工艺[J].中药材,2016,39(6):1365-1368.
[11] 罗晓梅,张吟,黄丹丹,等.紫外可见分光光度法测定无花果叶提取物中总黄酮的含量[J].中国药房,2015,26(15):2111-2113.
[12] 韦献雅,殷丽琴,钟成,等.DPPH法评价抗氧化活性研究进展[J].食品科学,2014,35(9):317-322.
[13] 成兰英,梁书凤,张治强.DPPH法研究麦冬提取物抗氧化活性[J].精细化工,2012,29(9):870-874.