温里药挥发油的皮肤刺激性考察
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摘要
目的:考察温里药挥发油的皮肤刺激性。方法:以3种温里药挥发油(肉桂挥发油、丁香挥发油、高良姜挥发油为研究对象,比较三者的皮肤细胞毒性和在体皮肤刺激性,并利用皮肤测试板预测这3种中药挥发油皮肤刺激性。结果:3种中药挥发油对人皮肤成纤维细胞(HSF)的毒性相差显著,肉桂挥发油、高良姜挥发油、丁香挥发油的半数抑制率(IC50)分别为(11. 16±0. 28),(53. 33±1. 71),(226. 70±17. 61) mg·L~(-1);在体皮肤刺激性评价表明,3种温里药挥发油对皮肤刺激性的排序为肉桂挥发油>丁香挥发油>高良姜挥发油;皮肤测试板评价3种挥发油刺激性结果与在体皮肤刺激性结果一致。结论:肉桂挥发油、丁香挥发油以及高良姜挥发油的皮肤细胞毒性评价结果与其在体皮肤刺激性结果不一致,采用皮肤测试板有望能够准确预测挥发油的在体皮肤刺激性。
Objective: To investigate the skin irritation of essential oils( EOs) extracted from interiorwarming medicines. Method: Three EOs from interior-warming medicines( Cinnamomi Cortex,Caryophylli Flos and Alpiniae Officinarum Rhizoma) were selected as research objects. The in vitro skin cytotoxicity and in vivo skin irritation of these EOs were determined and compared. Moreover,the skin irritation was also predicted by the novel skin test panels. Result: Toxicity of these three EOs to human skin fibroblasts( HSF) was significantly different,half-inhibitory concentration( IC50) values of EOs from Cinnamomi Cortex,Alpiniae Officinarum Rhizoma and Caryophylli Flos were( 11. 16 ± 0. 28),( 53. 33 ± 1. 71),( 226. 70 ± 17. 61) mg·L~(-1),respectively. However,in vivo skin irritation evaluation showed that the local toxicity of these three EOs was in the order of EO of Cinnamomi Cortex > EO of Caryophylli Flos > EO of Alpiniae Officinarum Rhizoma. The evaluation results of skin test panels for these three EOs were in accordance with the results of in vivo skin irritation evaluation. Conclusion: Toxicity of these three EOs against skin cells in vitro is inconsistent with their in vivo skin irritation. Skin test panels are expected to be able to accurately predict in vivo skin irritation of EOs instead of cytotoxicity evaluation.
Objective: To investigate the skin irritation of essential oils( EOs) extracted from interiorwarming medicines. Method: Three EOs from interior-warming medicines( Cinnamomi Cortex,Caryophylli Flos and Alpiniae Officinarum Rhizoma) were selected as research objects. The in vitro skin cytotoxicity and in vivo skin irritation of these EOs were determined and compared. Moreover,the skin irritation was also predicted by the novel skin test panels. Result: Toxicity of these three EOs to human skin fibroblasts( HSF) was significantly different,half-inhibitory concentration( IC50) values of EOs from Cinnamomi Cortex,Alpiniae Officinarum Rhizoma and Caryophylli Flos were( 11. 16 ± 0. 28),( 53. 33 ± 1. 71),( 226. 70 ± 17. 61) mg·L~(-1),respectively. However,in vivo skin irritation evaluation showed that the local toxicity of these three EOs was in the order of EO of Cinnamomi Cortex > EO of Caryophylli Flos > EO of Alpiniae Officinarum Rhizoma. The evaluation results of skin test panels for these three EOs were in accordance with the results of in vivo skin irritation evaluation. Conclusion: Toxicity of these three EOs against skin cells in vitro is inconsistent with their in vivo skin irritation. Skin test panels are expected to be able to accurately predict in vivo skin irritation of EOs instead of cytotoxicity evaluation.
引文
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[11]李钰,姚俊宏,殳叶婷,等.肉桂油与桂皮醛体外透皮吸收促进作用比较研究[J].中国中药杂志,2018,43(17):3493-3497.
[12] Mendanha S A,Moura S S,Anjos J L,et al. Toxicity of terpenes on fibroblast cells compared to their potential and increase in erythrocyte membrane fluidity[J].Toxicol in Vitro,2013,27(1):323-329.
[13] CHEN J,YAO J H,MA Z Y,et al. Delivery of fluorescent-labeled cyclodextrin by liposomes:role of transferrin modification and phosphatidylcholine composition[J]. J Liposome Res,2017,27(1):21-31.
[14]程娜娜,陆兔林,陈军,等.莪术油复合磷脂脂质体的制备工艺[J].中国实验方剂学杂志,2010,16(18):14-17.
[2]姚俊宏,蒋秋冬,陈军,等. 21种辛味中药挥发油透皮促渗效果的药性规律分析[J].中国实验方剂学杂志,2018,24(1):1-7.
[3]杨文国,姚俊宏,陈军,等. 33种辛味中药挥发油皮肤细胞毒性与药性特征的关联性研究[J].南京中医药大学学报,2017,33(6):597-602.
[4]郑声星,金胜威,连庆泉.成纤维细胞参与炎症发生和消退[J].生命的化学,2010,30(3):350-353.
[5] YI Q F,YAN J,TANG S Y,et al. Effect of borneol on the transdermal permeation of drugs with differing lipophilicity and molecular organization of stratum corneum lipids[J]. Drug Dev Ind Pharm,2016,42(7):1086-1093.
[6] Ahad A,Aqil M,Ali A. Investigation of antihypertensive activity of carbopol valsartan transdermal gel containing1,8-cineole[J]. Int J Biol Macromol,2014,64(2):144-149.
[7] Welss T,Basketter D A,Schrder K R. In vitro skin irritation:facts and future. State of the art review of mechanisms and models[J]. Toxicol in Vitro,2004,18(3):231-243.
[8] MA M,DI H J,ZHANG H,et al. Development of phospholipid vesicle-based permeation assay models capable of evaluating percutaneous penetration enhancing effect[J]. Drug Dev Ind Pharm,2017,43(12):2055-2063.
[9]陈军,张会,马敏,等.一种评价皮肤刺激性的方法、装置及其应用:中国,ZL201610406817. 8[P]. 2017-11-28.
[10]蒋秋冬,吴叶鸣,张会等.中药挥发油透皮促渗布洛芬的药动学及体内外相关性研究[J].中国中药杂志,2016,41(23):4362-4367.
[11]李钰,姚俊宏,殳叶婷,等.肉桂油与桂皮醛体外透皮吸收促进作用比较研究[J].中国中药杂志,2018,43(17):3493-3497.
[12] Mendanha S A,Moura S S,Anjos J L,et al. Toxicity of terpenes on fibroblast cells compared to their potential and increase in erythrocyte membrane fluidity[J].Toxicol in Vitro,2013,27(1):323-329.
[13] CHEN J,YAO J H,MA Z Y,et al. Delivery of fluorescent-labeled cyclodextrin by liposomes:role of transferrin modification and phosphatidylcholine composition[J]. J Liposome Res,2017,27(1):21-31.
[14]程娜娜,陆兔林,陈军,等.莪术油复合磷脂脂质体的制备工艺[J].中国实验方剂学杂志,2010,16(18):14-17.