越桔VcWRKY33基因的分离及其响应胁迫的表达分析
|
摘要
以南高丛越桔"雷格西"Vaccinium corymbosum ‘Legacy’为试材,克隆与抗逆相关的WRKY基因,分析其表达模式及其对高盐、低温、渗透(PEG6000)和水杨酸处理的响应。结果表明,克隆获得越桔VcWRKY33基因,其编码蛋白含有2个WRKYGQK结构域和1个C2H2锌指结构。VcWRKY33在不同组织器官中均可表达,但表达量差异明显,在幼叶中表达量较高。该基因明显受高盐、低温、PEG 6000和水杨酸诱导表达。VcWRKY33在越桔的抗盐及抗寒等过程中可能起重要作用。
引文
[1]李亚东,孙海悦,陈丽.我国蓝莓产业发展报告[J].中国果树,2016(5):1-10
[2] BERKHEIMER S F,HANSON E.Deicing salts reduce cold hardiness and increase flower bud mortality of highbush blueberry[J].Journal of the American Society for Horticultural Science,2006,131(1):1-16
[3] WRIGHT G C,PATTEN K D,DREW M C.Salinity and supplemental calcium influence growth of rabbiteye and southern highbush blueberry[J].Journal of the American Society for Horticultural Science,1992,117(5):749-756
[4] MICHEL M,CHRISTIAN P.Evidence of droughtsentive periods from flowering to maturity on highbush blueberry[J].Sciential Horticultural,2001,89:23-40
[5]吴林,李亚东,张志东,等.淹水、干旱条件下北空越桔生理反应的研究[J].果树学报,1998,15(2):137-140
[6]李亚东,卢春雨,裴嘉博.低温伤害对越桔开花坐果的影响及成因分析[J].中国果树,2008(4):26-28
[7]乌凤章,王贺新,韩慧,等.防寒措施对越桔越冬微环境和越冬性的影响[J].果树学报,2012,29(2):278-282
[8] JIANG Y Q,DEYHOLOS M K.Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes[J].BMC Plant Biology,2006,6:25
[9] MARE C,MAZZUCOTELLI E,CROSATTI C,et al.Hv-WRKY38:a new trans-cription factor involved in cold and drought response in barley[J].Plant Molecular Biology,2004,55:399-416
[10]TROGNITZ F,MANOSALVA P,GYSIN R,et al.Plant defense genes associated with quantitative resistance to potato late blight in Solanum phureja×dihaploid S.tuberosum hybrids[J].Molecular Plant Microbe Interact,2002,15:587-597
[11]ZHU D,CHE Y M,XIAO P L,et al.Functional anaylsis of a grape WRKY30gene in drought resistance[J].Plant Cell,Tissue and Organ Culture,2018,132(3):449-459
[12]LUI S,LUO C G,ZHU L M,et al.Identification and expression analysis of WRKY transcription factor genes in response to fungal pathogen and hormone treatments in apple(Malus domestica)[J].Jounrl of Plant Biology,2017,60(2):215-230
[13] SONG Y,LIU H D,ZHOU Q,et al. Highthroughput sequencing of highbush blueberry transcriptome and analysis of basic helix-loop-helix transcription factors[J].Journal of Integrative Agriculture,2017,16(3):591-604
[14]EULGEM T,GUSHTON P J,ROBATZEK S,et al.The WRKY superfamily of plant transcriptional factors[J].Trends in Plant Science,2000,5:199-206
[15]JIANG Y Q,DEYHOLOS M.Functional characterization of Arabidopsis NaCl-inducible WRKY25and WRKY33 transcription factors in abiotic stresses[J].Plant Molecular Biology,2009,69:91-105
[16] MATSUI A,ISHIDA J,MOROSAWA T,et al.Arabidopsis transcriptome analysis under drought,cold,high-salinity and ABA treatment conditions using a tiling array[J].Plant Cell Phydiol,2008,49:1135-1149
[17]MIURA K,TADA Y.Regulation of water,salinity,and cold stress response by salicylic acid[J].Frontiers in Plant Science,2014,5(2):4
[18]LIU X Q,BAI X Q,QIAN Q,et al.OsWRKY03,a rice transcription factor,is involved in rice defense response[J].Journal of Plant Physiology,2007,164:969-979
[19]RYU H S,HAN M H,LEE S K,et al.A comprehensive expression analysis of the WRKY gene superfamily in rice plants during defense response[J].Plant Cell Reporter,2006,25:836-847
[20]ROBATZEK S,SOMSSICH I E.A new member of the Arabidopsis WRKY transcription factor family,AtWRKY6,is associated with both senescence-and defense-related processes[J].The Plant Journal,2001,28:123-133
[2] BERKHEIMER S F,HANSON E.Deicing salts reduce cold hardiness and increase flower bud mortality of highbush blueberry[J].Journal of the American Society for Horticultural Science,2006,131(1):1-16
[3] WRIGHT G C,PATTEN K D,DREW M C.Salinity and supplemental calcium influence growth of rabbiteye and southern highbush blueberry[J].Journal of the American Society for Horticultural Science,1992,117(5):749-756
[4] MICHEL M,CHRISTIAN P.Evidence of droughtsentive periods from flowering to maturity on highbush blueberry[J].Sciential Horticultural,2001,89:23-40
[5]吴林,李亚东,张志东,等.淹水、干旱条件下北空越桔生理反应的研究[J].果树学报,1998,15(2):137-140
[6]李亚东,卢春雨,裴嘉博.低温伤害对越桔开花坐果的影响及成因分析[J].中国果树,2008(4):26-28
[7]乌凤章,王贺新,韩慧,等.防寒措施对越桔越冬微环境和越冬性的影响[J].果树学报,2012,29(2):278-282
[8] JIANG Y Q,DEYHOLOS M K.Comprehensive transcriptional profiling of NaCl-stressed Arabidopsis roots reveals novel classes of responsive genes[J].BMC Plant Biology,2006,6:25
[9] MARE C,MAZZUCOTELLI E,CROSATTI C,et al.Hv-WRKY38:a new trans-cription factor involved in cold and drought response in barley[J].Plant Molecular Biology,2004,55:399-416
[10]TROGNITZ F,MANOSALVA P,GYSIN R,et al.Plant defense genes associated with quantitative resistance to potato late blight in Solanum phureja×dihaploid S.tuberosum hybrids[J].Molecular Plant Microbe Interact,2002,15:587-597
[11]ZHU D,CHE Y M,XIAO P L,et al.Functional anaylsis of a grape WRKY30gene in drought resistance[J].Plant Cell,Tissue and Organ Culture,2018,132(3):449-459
[12]LUI S,LUO C G,ZHU L M,et al.Identification and expression analysis of WRKY transcription factor genes in response to fungal pathogen and hormone treatments in apple(Malus domestica)[J].Jounrl of Plant Biology,2017,60(2):215-230
[13] SONG Y,LIU H D,ZHOU Q,et al. Highthroughput sequencing of highbush blueberry transcriptome and analysis of basic helix-loop-helix transcription factors[J].Journal of Integrative Agriculture,2017,16(3):591-604
[14]EULGEM T,GUSHTON P J,ROBATZEK S,et al.The WRKY superfamily of plant transcriptional factors[J].Trends in Plant Science,2000,5:199-206
[15]JIANG Y Q,DEYHOLOS M.Functional characterization of Arabidopsis NaCl-inducible WRKY25and WRKY33 transcription factors in abiotic stresses[J].Plant Molecular Biology,2009,69:91-105
[16] MATSUI A,ISHIDA J,MOROSAWA T,et al.Arabidopsis transcriptome analysis under drought,cold,high-salinity and ABA treatment conditions using a tiling array[J].Plant Cell Phydiol,2008,49:1135-1149
[17]MIURA K,TADA Y.Regulation of water,salinity,and cold stress response by salicylic acid[J].Frontiers in Plant Science,2014,5(2):4
[18]LIU X Q,BAI X Q,QIAN Q,et al.OsWRKY03,a rice transcription factor,is involved in rice defense response[J].Journal of Plant Physiology,2007,164:969-979
[19]RYU H S,HAN M H,LEE S K,et al.A comprehensive expression analysis of the WRKY gene superfamily in rice plants during defense response[J].Plant Cell Reporter,2006,25:836-847
[20]ROBATZEK S,SOMSSICH I E.A new member of the Arabidopsis WRKY transcription factor family,AtWRKY6,is associated with both senescence-and defense-related processes[J].The Plant Journal,2001,28:123-133