大鲵源致病性弗氏柠檬酸杆菌的分离、鉴定和药物敏感性
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摘要
为鉴定患病大鲵(Andrias davidianus)病原菌,从大鲵肠、胃、脾脏中分离得到菌株int1705,分离菌株经形态特征观察、生理生化特性测定、16S rRNA和aspC管家基因序列测定及系统发育学分析,鉴定为弗氏柠檬酸杆菌(Citrobacter freundii)。经人工感染,结果显示:菌株int1705能使健康大鲵发病并致死,半致死浓度(LD_(50))为3.16×10~(6 )CFU/mL,且发病症状与自然发病相似。药敏试验结果显示,菌株int1705对多粘菌素B、头孢他啶、诺氟沙星、氧氟沙星、复方新诺明等10种药物敏感。
The int1705 strain was isolated from the lesion site of intestine,stomach,spleen of Andrias davidianus to identify the pathogenic agent of the diseased.The int1705 was identified as Citrobacter freundii based on morphological and biochemical identification,together with 16 S rRNA and aspC gene sequencing.Experimental infection results showed that int1705 exhibited virulence to Andrias davidianus with an LD_(50) value of 3.16×10~(6 )CFU/mL.Symptoms were similar to those natural cases.Antimicrobial susceptibility test results showed that the int1705 was sensitive to 10 kinds of antibiotics,such as polymyxin B,Ceftazidime and norfloxacin,etc.
The int1705 strain was isolated from the lesion site of intestine,stomach,spleen of Andrias davidianus to identify the pathogenic agent of the diseased.The int1705 was identified as Citrobacter freundii based on morphological and biochemical identification,together with 16 S rRNA and aspC gene sequencing.Experimental infection results showed that int1705 exhibited virulence to Andrias davidianus with an LD_(50) value of 3.16×10~(6 )CFU/mL.Symptoms were similar to those natural cases.Antimicrobial susceptibility test results showed that the int1705 was sensitive to 10 kinds of antibiotics,such as polymyxin B,Ceftazidime and norfloxacin,etc.
引文
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[9] 沈锦玉,顾志敏,潘晓艺,等.红螯螯虾弗氏柠檬酸杆菌病病原的分离与鉴定[J].中国水产科学,2005,12(2):197-200.
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[12] 陈言峰,马文山,陈文慧,等.斑鳢嗜水气单胞菌病病原的鉴定及其病理组织学的研究[J].大连海洋大学学报,2011,26(6):514-520.
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[15] 卢君辉,孙佳佳,董世龙,等.乌翅真鲨弗氏柠檬酸杆菌的分离与鉴定[J].海洋湖沼通报,2015,(4):83-86.
[16] 陈翠珍,房海,葛慕湘,等.锦鲤弗氏柠檬酸杆菌的鉴定[J].河北科技师范学院学报,2011,25(4):21-25.
[17] 王利,魏勇.鲫鱼弗氏柠檬酸杆菌的鉴定及系统发育分析[J].水产科学,2012,31(8):481-484.
[18]Titball R W.Bacterial phospholipases[J].Trends Microbiol,1997,5(7):265.
[19] Poirel L,Ros A,Carricajo A,et al.Extremely drug-resistant Citrobacter freundii isolate producing NDM-1 and other carbapenemases identified in a patient returning from India[J].Antimicrob Agents Chemother,2011,55(1):447-448.
[20] 孙洋,郭学军,周博,等.东北虎出血性腹泻病原分离与鉴定[J].中国兽医杂志,2008,44(9):89-90.
[2] 毛烁君,斯广杰,任晓金,等.一株多重耐药中华鳖源弗氏柠檬酸杆菌的分离鉴定[J].淡水渔业,2015,45(2):102-106.
[3]Trinidade A,Sekhawat V,Andreou Z,et al.Citrobacter freundii causing pharyngitis and secondary retropharyngeal abscess with intrathoracic extension to the diaphragm:minimally invasive management of a rare case[J].J Laryngol Otol,2010,124(5):296-300.
[4] 薛巧,赵战勤,刘会胜,等.弗氏柠檬酸杆菌对动物和人致病性研究进展[J].动物医学进展,2015,36(7):81-85.
[5] Sato N,Yamane N,Kawamura T.Systemic Citrobacter freundii infection among sunfish Mola mola in Matsushima Aquarium[J].Nihon Suisan Gakkaishi,1982,48(11):1551-1557.
[6]杨移斌,夏永涛,赵蕾,等.鲟源弗氏柠檬酸杆菌分离鉴定及药敏特性研究[J].水生生物学报,2013,37(4):766-771.
[7]杨方园,关瑞章,李忠琴,等.花鳗鲡病原菌弗氏柠檬酸杆菌的鉴定[J].集美大学学报:自然科学版,2013,18(2):81-87.
[8]李华,邢殿楼,白国福,等.弗氏柠檬酸杆菌对河蟹致病性的研究[J].水生生物学报,2001,25(3):217-223.
[9] 沈锦玉,顾志敏,潘晓艺,等.红螯螯虾弗氏柠檬酸杆菌病病原的分离与鉴定[J].中国水产科学,2005,12(2):197-200.
[10] 林启存,朱丽敏,李忠全,等.中华鳖弗氏柠檬酸杆菌败血症病原分离鉴定与药敏试验[J].水产科学,2008,27(1):42-43.
[11] 陈红莲,宋光同,何吉祥,等.克氏原螯虾弗氏柠檬酸杆菌的分离鉴定与药敏试验[J].淡水渔业,2014,44(1):73-77.
[12] 陈言峰,马文山,陈文慧,等.斑鳢嗜水气单胞菌病病原的鉴定及其病理组织学的研究[J].大连海洋大学学报,2011,26(6):514-520.
[13] Rozhavin M A,Sologub V V.Determination of the lecithinase activity of Pseudomonas aeruginosa[J].Lab Delo,1980,(11):681-682.
[14]张冬星,康元环,田佳鑫,等.团头鲂致病性弗氏柠檬酸杆菌的分离鉴定及药敏试验[J].中国兽医科学,2016,46(12):1589-1595.
[15] 卢君辉,孙佳佳,董世龙,等.乌翅真鲨弗氏柠檬酸杆菌的分离与鉴定[J].海洋湖沼通报,2015,(4):83-86.
[16] 陈翠珍,房海,葛慕湘,等.锦鲤弗氏柠檬酸杆菌的鉴定[J].河北科技师范学院学报,2011,25(4):21-25.
[17] 王利,魏勇.鲫鱼弗氏柠檬酸杆菌的鉴定及系统发育分析[J].水产科学,2012,31(8):481-484.
[18]Titball R W.Bacterial phospholipases[J].Trends Microbiol,1997,5(7):265.
[19] Poirel L,Ros A,Carricajo A,et al.Extremely drug-resistant Citrobacter freundii isolate producing NDM-1 and other carbapenemases identified in a patient returning from India[J].Antimicrob Agents Chemother,2011,55(1):447-448.
[20] 孙洋,郭学军,周博,等.东北虎出血性腹泻病原分离与鉴定[J].中国兽医杂志,2008,44(9):89-90.