萃取浓缩沼液对尖孢镰刀菌和腐皮镰孢菌抑制效果的影响研究
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摘要
为了研究不同有机溶剂萃取浓缩沼液对尖孢镰刀菌(Fusariumoxysporum)和腐皮镰孢菌(Fusariumsolani)抑制效果的影响,该实验采用6种常见的有机溶剂(乙酸乙酯、苯、乙醚、甲苯、环己烷、石油醚)对已经去除80%水分的沼液浓缩液进行萃取后再浓缩,将最终的萃取浓缩物进行抑菌试验。结果显示:6种有机溶剂萃取得到的萃取浓缩物都对尖孢镰刀菌和腐皮镰孢菌有抑制作用,且萃取浓缩物的抑制效果强于浓缩液、原沼液和萃取后剩余浓缩液的抑制效果。该实验可为沼液防治三七根腐病的利用奠定基础和依据。
In order to study the influences of different organic solvent extracted and concentrated biogas slurry on the inhibition effect of Fusariumoxysporum and Fusariumsolani, in this experiment, six common organic solvents(ethyl acetate, benzene, diethyl ether, toluene, cyclohexane, petroleum ether) were used to extract and concentrate a concentrated biogas slurry which had in advance removed 80% of the water. The final extraction concentrated slurry was tested for bacteriostasis. The results showed that these extraction concentrated slurries by six organic solvents all could inhibite Fusariumoxysporum and Fusariumsolani, and they had stronger inhibition effect than those of the starting slurry concentrate(with 80% water removed), the original biogas slurry, and the remained liquid after the extraction. This experiment can lay a foundation and basis for the utilization of biogas slurry to control the root rot of Panaxnotoginseng.
In order to study the influences of different organic solvent extracted and concentrated biogas slurry on the inhibition effect of Fusariumoxysporum and Fusariumsolani, in this experiment, six common organic solvents(ethyl acetate, benzene, diethyl ether, toluene, cyclohexane, petroleum ether) were used to extract and concentrate a concentrated biogas slurry which had in advance removed 80% of the water. The final extraction concentrated slurry was tested for bacteriostasis. The results showed that these extraction concentrated slurries by six organic solvents all could inhibite Fusariumoxysporum and Fusariumsolani, and they had stronger inhibition effect than those of the starting slurry concentrate(with 80% water removed), the original biogas slurry, and the remained liquid after the extraction. This experiment can lay a foundation and basis for the utilization of biogas slurry to control the root rot of Panaxnotoginseng.
引文
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[3] 杨永建,崔秀明,杨涛,等.文山三七规范化种植及其发展对策[J].云南农业大学学报,2008,23(3):402-406.
[4] 李冠烈.三七的现代研究与进展(一)[J].世界中西结合杂志,2008,(3-10):619-623.
[5] 郑光植,杨崇仁,三七生物学及其应用[M].北京:科学出版社,1994:14.
[6] 吴凤云.三七连作对土壤酶活及土壤化学性质的影响研究[D].昆明:云南师范大学,2017.
[7] 魏丹丽.三七根腐病绿色防治技术体系研发[D].昆明:云南农业大学,2017.
[8] 缪作清,李世东,刘杏忠,等.三七根腐病病原研究[J].中国农业科学,2006,39(7):1371-1378.
[9] 张无敌,沼气发酵残留物利用基础[M].昆明:云南科技出版社,20024.
[10] 沈其林,单胜道,周健驹,等.猪粪发酵沼液成分测定与分析[J].中国沼气,2014,32(3):83-86.
[11] 杨顺强.不同pH值PDA培养基对平菇、香菇母种培养的影响[J].现代农业科技,2016,16:67-68.
[12] 刘丁才.沼液中萜类化合物新型结构的发现与生物活性的研究[D].上海:上海海洋大学,2013.
[13] 方梦萦,张无敌,尹芳,等.不同来源沼液对根霉抑制作用的研究[J].云南师范大学学报(自然科学版),2014,34(3):29-32.
[14] 方梦萦,张无敌,尹芳,等.沼气发酵液对烟草赤星菌抑菌作用的研究[J].云南师范大学学报(自然科学版),2011,31(增刊):115-118.