高分辨熔解技术在食品真实溯源检测中的应用
|
摘要
随着食品工业的发展,食品供应链和消费链的延长,以经济利益为驱动的食品掺假行为日益增多并严重侵害了消费者的权益和身体健康.食品的安全追溯可以有效控制食品的质量安全,为食品安全提供保障.在众多的溯源技术中,以核酸为靶标的PCR(Polymerase Chain Reaction)技术,克服了蛋白质加工处理易变性的缺点而被广泛地应用于食品真伪鉴别中,其中高分辨率熔解曲线(High Resolution Melting,HRM)分析是一种新型的PCR检测技术,具有操作简单、成本低廉、高通量、灵敏度强、特异性高和真正闭管操作等优点,目前已逐渐应用于食品掺假以及产地溯源检测.本文总结了影响HRM食品分析的关键因素以及HRM在食品鉴定中的应用情况,包括在肉制品、乳制品、谷物类、水果饮料等食品鉴定中的应用,最后分析了HRM在食品真实溯源检测中的优缺点以及发展趋势.
With the development of the food industry,and the extension of the food supply and consumption chain,food-related adulteration driven by economic interests has seriously influence on the rights and health of consumers.Food safety traceability can effectively control the quality and safety of food and provide guarantee for food safety.Among many traceable technologies,nucleic acid-based PCR technology has been widely used in food assays because it overcomes the variability of protein processing.As a new type of PCR technology,the highresolution dissolution(HRM)has advantages of simple operation,low cost,high throughput,high sensitivity,high specificity,and true closed tube operation and is gradually used in food authentication.This paper summarizes the key factors affecting HRM food assay and the applications of HRM to identify meat products,dairy products,cereals,and fruit drinks.Finally,the advantages and limitation of HRM and its potential in food analysis are also discussed.
With the development of the food industry,and the extension of the food supply and consumption chain,food-related adulteration driven by economic interests has seriously influence on the rights and health of consumers.Food safety traceability can effectively control the quality and safety of food and provide guarantee for food safety.Among many traceable technologies,nucleic acid-based PCR technology has been widely used in food assays because it overcomes the variability of protein processing.As a new type of PCR technology,the highresolution dissolution(HRM)has advantages of simple operation,low cost,high throughput,high sensitivity,high specificity,and true closed tube operation and is gradually used in food authentication.This paper summarizes the key factors affecting HRM food assay and the applications of HRM to identify meat products,dairy products,cereals,and fruit drinks.Finally,the advantages and limitation of HRM and its potential in food analysis are also discussed.
引文
[1]李敏,凌超,邬琦沁,等.DNA条形码技术在食品溯源中的应用[J].乳业科学与技术,2014,37(5):25-30.
[2]马慧鋆,余冰雪,李妍,等.食品溯源技术研究进展[J].食品与发酵工业,2017,43(5):277-284.
[3]郭振华,陈换美.食品溯源技术研究现状及分析[J].新疆农机化,2017(6):34-37.
[4]宋君,雷绍荣,郭灵安,等.DNA指纹技术在食品掺假、产地溯源检验中的应用[J].安徽农业科学,2012,40(6):3 226-3 228.
[5]赵燕,李建科.现代技术在食品真伪和产地鉴别中的应用[J].食品研究与开发,2007,28(9):157-161.
[6]李茹,廖灿,李东至,等.高分辨熔解曲线分析技术检测中国人常见β地中海贫血基因突变[J].中国优生与遗传杂志,2011,19(7):19-21,137.
[7]陈斌,雷秀霞,周小棉.高分辨率熔解曲线技术及其在分子诊断中的应用进展[J].分子诊断与治疗杂志,2009,1(2):120-124.
[8]潘登.高分辨率熔解曲线分析技术(HRM)系统的建立及其在基因多态性和突变检测方面的应用[D].上海:复旦大学,2013.
[9]王冬梅,李俊,杨红敏,等.高分辨率熔解曲线HRM在SNP检测中的应用[J].基因组学与应用生物学,2015,34(4):892-895.
[10]White H E,Hall V J,Cross N C.Methylation-sensitive high-resolution melting-curve analysis of the SNRPN gene as a diagnostic screen for Prader-Willi and Angelman syndromes[J].Clinical Chemistry,2007,53(11):1 960-1 962.
[11]朱岩芳,张炜,胡晋,等.高分辨率熔解曲线分析(HRM)及其在植物种质资源鉴定中的应用[J].种子,2013,32(10):57-60.
[12]Pereira L,Gomes S,Barrias S,et al.Applying high-resolution melting(HRM)technology to olive oil and wine authenticity[J].Food Research International,2018,103:170-181.
[13]Druml B,Cichna Markl M.High resolution melting(HRM)analysis of DNA-Its role and potential in food analysis[J].Food Chemistry,2014,158(9):245-254.
[14]饶丹,嘎利兵嘎,郭小权,等.HRM分析方法中不同扩增片段、染料和仪器的比较[J].江西农业大学学报,2015,37(1):169-175.
[15]刘自增,吴周良,阎萍,等.高分辨率熔解曲线分析应用的研究进展[J].中国畜牧兽医,2013,40(8):105-111.
[16]Radvanszky J,Surovy M,Nagyova E,et al.Comparison of different DNA binding fluorescent dyes for applications of high-resolution melting analysis[J].Clinical Biochemistry,2015,48(9):609-616.
[17]郭心灵,尤崇革.高分辨率熔解曲线技术常用的仪器和荧光染料[J].分子诊断与治疗杂志,2012,4(1):50-53.
[18]Herrmann M G,Durtschi J D,Bromley L K,et al.Amplicon DNA melting analysis for mutation scanning and genotyping:Cross-platform comparison of instruments and dyes[J].Clinical Chemistry,2006,52(3):494-503.
[19]Herrmann M G,Durtschi J D,Wittwer C T,et al.Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping[J].Clinical Chemistry,2007,53(8):1 544-1 548.
[20]Liao J,Yang L,Sheppard A M,et al.Comparison of DNA quality in raw and reconstituted milk during sterilization[J].Journal of Dairy Science,2017,101(1):1-7.
[21]Spaniolas S,Bazakos C,Ntourou T,et al.Use of lambda DNA as a marker to assess DNA stability in olive oil during storage[J].European Food Research and Technology,2008,227:175-179.
[22]Faria M A,Magalh2es A,Nunes M E,et al.High resolution melting of trnL,amplicons in fruit juices authentication[J].Food Control,2013,33(1):136-141.
[23]Ganopoulos I,Argiriou A,Tsaftaris A.Microsatellite high resolution melting(SSR-HRM)analysis for authenticity testing of protected designation of origin(PDO)sweet cherry products[J].Food Control,2011,22(3-4):532-541.
[24]Sakalar E,Abasiyanik M F,Bektik E,et al.Effect of heat processing on DNA quantification of meat species[J].Journal of Food Science,2012,77(9),40-44.
[25]Pereira L,Gomes S,Castro C,et al.High resolution melting(HRM)applied to wine authenticity[J].Food Chemistry,2017,216:80-86.
[26]Tomas C,I M P L V O Ferreira,Faria M A.Codfish authentication by a fast short amplicon high resolution melting analysis(SA-HRMA)method[J].Food Control,2017,71:255-263.
[27]Jaakola L,Suokas M,Hggman H.Novel approaches based on DNA barcoding and high-resolution melting of amplicons for authenticity analyses of berry species[J].Food Chemistry,2010,123(2):494-500.
[28]郭瑜,赖松家.线粒体DNA的研究及其应用[J].畜禽业,2004(11):64-66.
[29]闫华超.动物线粒体DNA分析技术及其应用[J].生物技术通讯,2010,21(2):280-283,294.
[30]Robin E D,Wong R.Mitochondrial DNA molecules and virtual number of mitochondria per cell in mammalian cells[J].Journal of Cellular Physiology,1988,136(3):507-513.
[31]Sarri C,Stamatis C,Sarafidou T,et al.A new set of 16S rRNA universal primers for identification of animal species[J].Food Control,2014,43(5):35-41.
[32]Lopez Oceja A,Gamarra D,Borragan S,et al.New cyt b gene universal primer set for forensic analysis[J].Forensic Science International Genetics,2016,23:159-165.
[33]何玮玲,张驰,杨静,等.食品中4种肉类成分多重PCR的快速鉴别方法[J].中国农业科学,2012,45(9):1 873-1 880.
[34]Mafra I,urea Roxo,I M P L V O Ferreira,et al.A duplex polymerase chain reaction for the quantitative detection of cows′milk in goats′milk cheese[J].International Dairy Journal,2007,17(9):1 132-1 138.
[35]田金辉.食品中动物源性成分定性鉴定方法研究[D].杨陵:西北农林科技大学,2011.
[36]Kitano T,Umetsu K,Tian W,et al.Two universal primer sets for species identification among vertebrates[J].International Journal of Legal Medicine,2007,121(5):423-427.
[37]宋洁,张小芳,刁雪淘,等.线粒体DNA12SrRNA、16SrRNA研究进展[J].安徽农学通报,2008,14(9):42-43.
[38]Taberlet P,Coissac E,Pompanon F,et al.Power and limitations of the chloroplast trnL(UAA)intron for plant DNA barcoding[J].Nucleic Acids Research,2007,35(3):1-8.
[39]付涛,王志龙,钱萍仙,等.高等植物DNA条形码最新研究进展及其应用[J].核农学报,2016,30(5):887-896.
[40]杨慧洁,杨世海,张淑丽,等.药用植物DNA条形码研究进展[J].中草药,2014,45(18):2 581-2 587.
[41]Sakaridis I,Ganopoulos I,Argiriou A,et al.A fast and accurate method for controlling the correct labeling of products containing buffalo meat using High Resolution Melting(HRM)analysis[J].Meat Science,2013,94(1):84-88.
[42]Klomtong P,Phasuk Y,Duangjinda M.Animal species identification through high resolution melting real time PCR(HRM)of the mitochondrial 16SrRNA gene[J].Annals of Animal Science,2016,16(2):415-424.
[43]Lopez Oceja A,Nu1ez C,Baeta M,et al.Species identification in meat products:A new screening method based on high resolution melting analysis of cyt b gene[J].Food Chemistry,2017,237:701-706.
[44]Naue J,Hansmann T,Schmidt U.High-resolution melting of 12SrRNA and cytochrome b DNA sequences for discrimination of species within distinct european animal families[J].Plos One,2014,9(12):1-17.
[45]Yang S,Li C,Wu Q,et al.High-resolution melting analysis:A promising molecular method for meat traceability[J].European Food Research&Technology,2014,239(3):473-480.
[46]Wu Q Y,Zhou G H,Yang S S,et al.SNP genotyping in sheep from northwest and east China for meat traceability[J].Journal of Consumer Protection&Food Safety,2017,12:1-6.
[47]Wu Q Y,Zhou G H,Yang S S,et al.Combined SNPs and miRNAs technologies for beef traceability[J].Journal of Food Safety,2017,37(4):1-11.
[48]Jilberto F,Araneda C,Larraín M A.High resolution melting analysis for identification of commercially-important Mytilus species[J].Food Chemistry,2017,229:716-720.
[49]Fernandes T J R,Costa J,Oliveira M B,et al.DNA barcoding coupled to HRM analysis as a new and simple tool for the authentication of Gadidae fish species[J].Food Chemistry,2017,230:49-57.
[50]Fernandes T J R,Costa J,Oliveira M B P P,et al.COI barcode-HRM as a novel approach for the discrimination of hake species[J].Fisheries Research,2017,197:50-59.
[51]Ganopoulos I,Sakaridis I,Argiriou A,et al.A novel closed-tube method based on high resolution melting(HRM)analysis for authenticity testing and quantitative detection in Greek PDO Feta cheese[J].Food Chemistry,2013,141(2):835-840.
[52]Sakaridis I,Ganopoulos I,Argiriou A,et al.High resolution melting analysis for quantitative detection of bovine milk in pure water buffalo mozzarella and other buffalo dairy products[J].International Dairy Journal,2013,28(1):32-35.
[53]Madesis P,Ganopoulos I,Anagnostis A,et al.The application of Bar-HRM(Barcode DNA-High Resolution Melting)analysis for authenticity testing and quantitative detection of bean crops(Leguminosae)without prior DNA purification[J].Food Control,2012,25(2):576-582.
[54]Ganopoulos I,Madesis P,Darzentas N,et al.Barcode High Resolution Melting(Bar-HRM)analysis for detection and quantification of PDO “Fava Santorinis”(Lathyrus clymenum)adulterants[J].Food Chemistry,2012,133(2):505-512.
[55]Ganopoulos I,Bosmali I,Madesis P,et al.Microsatellite genotyping with HRM(High Resolution Melting)analysis for identification of the PGI common bean variety plake megalosperma prespon[J].European Food Research&Technology,2012,234(3):501-508.
[56]Bosmali I,Ganopoulos I,Madesis P,et al.Microsatellite and DNA-barcode regions typing combined with High Resolution Melting(HRM)analysis for food forensic uses:A case study on lentils(Lens culinaris)[J].Food Research International,2012,46(1):141-147.
[57]Ganopoulos I,Argiriou A,Tsaftaris A.Adulterations in basmati rice detected quantitatively by combined use of microsatellite and fragrance typing with High Resolution Melting(HRM)analysis[J].Food Chemistry,2011,129(2):652-659.
[58]Ganopoulos I,Bazakos C,Madesis P,et al.Barcode DNA high-resolution melting(Bar-HRM)analysis as a novel close-tubed and accurate tool for olive oil forensic use[J].Journal of the Science of Food&Agriculture,2013,93(9):2 281-2 286.
[59]Vietina M,Agrimonti C,Marmiroli N.Detection of plant oil DNA using high resolution melting(HRM)post PCR analysis:A tool for disclosure of olive oil adulteration[J].Food Chemistry,2013,141(4):3 820-3 826.
[60]Montemurro C,Miazzi M M,Pasqualone A,et al.Traceability of PDO olive oil“Terra di Bari”using high resolution melting[J].Journal of Chemistry,2015,205(1):1-7.
[61]Distefano G,Caruso M,La M S,et al.High resolution melting analysis is a more sensitive and effective alternative to gel-based platforms in analysis of SSR-an example in citrus[J].Plos One,2012,7(8):1-11.
[62]Mackay J F,Wright C D,Bonfiglioli R G.A new approach to varietal identification in plants by microsatellite high resolution melting analysis:Application to the verification of grapevine and olive cultivars[J].Plant Methods,2008,4(1):1-10.
[63]张梦婷,孙伟,师玉华,等.基于HRM的常见伞形科香辛料快速鉴定[J].中国实验方剂学杂志,2018,24(6):26-31.
[64]Heejin J,Yeongdeuk J,Soungwoo P,et al.Identification of capsicum species using SNP markers based on high resolution melting analysis[J].Genome,2010,53(12):1 029-1 040.
[65]Liu Y,Xiang L,Zhang Y,et al.DNA barcoding based identification of hippophae species and authentication of commercial products by high resolution melting analysis[J].Food Chemistry 2018,242:62-67.
[66]Jiang C,Cao L,Yuan Y,et al.Barcoding melting curve analysis for rapid,sensitive,and discriminating authentication of saffron(Crocus sativus L.)from its adulterants[J].Biomed Research International,2014(1):1-10.
[67]Bosmali I,Ordoudi S A,Tsimidou M Z,et al.Greek PDO saffron authentication studies using species specific molecular markers[J].Food Research International 2017,100:899-907.
[68]王志科,鲁放,熊超,等.Bar-HRM技术在人参和西洋参药材鉴定中的应用研究[J].世界科学技术-中医药现代化,2016,18(2):191-195.
[69]陈媞颖,蒋超,袁媛,等.基于DNA熔解曲线技术的金银花种质鉴定[J].中国中药杂志,2016,41(24):4 572-4 578.
[70]熊超,李景剑,孙伟,等.HRM结合DNA条形码在苦杏仁对桃仁掺混检测中的应用[J].药学学报,2017,52(4):647-652.
[71]童宇茹,蒋超,黄璐琦,等.基于DNA熔解曲线分析技术的三七粉分子鉴定[J].药物分析杂志,2014,34(8):1 384-1 390.
[2]马慧鋆,余冰雪,李妍,等.食品溯源技术研究进展[J].食品与发酵工业,2017,43(5):277-284.
[3]郭振华,陈换美.食品溯源技术研究现状及分析[J].新疆农机化,2017(6):34-37.
[4]宋君,雷绍荣,郭灵安,等.DNA指纹技术在食品掺假、产地溯源检验中的应用[J].安徽农业科学,2012,40(6):3 226-3 228.
[5]赵燕,李建科.现代技术在食品真伪和产地鉴别中的应用[J].食品研究与开发,2007,28(9):157-161.
[6]李茹,廖灿,李东至,等.高分辨熔解曲线分析技术检测中国人常见β地中海贫血基因突变[J].中国优生与遗传杂志,2011,19(7):19-21,137.
[7]陈斌,雷秀霞,周小棉.高分辨率熔解曲线技术及其在分子诊断中的应用进展[J].分子诊断与治疗杂志,2009,1(2):120-124.
[8]潘登.高分辨率熔解曲线分析技术(HRM)系统的建立及其在基因多态性和突变检测方面的应用[D].上海:复旦大学,2013.
[9]王冬梅,李俊,杨红敏,等.高分辨率熔解曲线HRM在SNP检测中的应用[J].基因组学与应用生物学,2015,34(4):892-895.
[10]White H E,Hall V J,Cross N C.Methylation-sensitive high-resolution melting-curve analysis of the SNRPN gene as a diagnostic screen for Prader-Willi and Angelman syndromes[J].Clinical Chemistry,2007,53(11):1 960-1 962.
[11]朱岩芳,张炜,胡晋,等.高分辨率熔解曲线分析(HRM)及其在植物种质资源鉴定中的应用[J].种子,2013,32(10):57-60.
[12]Pereira L,Gomes S,Barrias S,et al.Applying high-resolution melting(HRM)technology to olive oil and wine authenticity[J].Food Research International,2018,103:170-181.
[13]Druml B,Cichna Markl M.High resolution melting(HRM)analysis of DNA-Its role and potential in food analysis[J].Food Chemistry,2014,158(9):245-254.
[14]饶丹,嘎利兵嘎,郭小权,等.HRM分析方法中不同扩增片段、染料和仪器的比较[J].江西农业大学学报,2015,37(1):169-175.
[15]刘自增,吴周良,阎萍,等.高分辨率熔解曲线分析应用的研究进展[J].中国畜牧兽医,2013,40(8):105-111.
[16]Radvanszky J,Surovy M,Nagyova E,et al.Comparison of different DNA binding fluorescent dyes for applications of high-resolution melting analysis[J].Clinical Biochemistry,2015,48(9):609-616.
[17]郭心灵,尤崇革.高分辨率熔解曲线技术常用的仪器和荧光染料[J].分子诊断与治疗杂志,2012,4(1):50-53.
[18]Herrmann M G,Durtschi J D,Bromley L K,et al.Amplicon DNA melting analysis for mutation scanning and genotyping:Cross-platform comparison of instruments and dyes[J].Clinical Chemistry,2006,52(3):494-503.
[19]Herrmann M G,Durtschi J D,Wittwer C T,et al.Expanded instrument comparison of amplicon DNA melting analysis for mutation scanning and genotyping[J].Clinical Chemistry,2007,53(8):1 544-1 548.
[20]Liao J,Yang L,Sheppard A M,et al.Comparison of DNA quality in raw and reconstituted milk during sterilization[J].Journal of Dairy Science,2017,101(1):1-7.
[21]Spaniolas S,Bazakos C,Ntourou T,et al.Use of lambda DNA as a marker to assess DNA stability in olive oil during storage[J].European Food Research and Technology,2008,227:175-179.
[22]Faria M A,Magalh2es A,Nunes M E,et al.High resolution melting of trnL,amplicons in fruit juices authentication[J].Food Control,2013,33(1):136-141.
[23]Ganopoulos I,Argiriou A,Tsaftaris A.Microsatellite high resolution melting(SSR-HRM)analysis for authenticity testing of protected designation of origin(PDO)sweet cherry products[J].Food Control,2011,22(3-4):532-541.
[24]Sakalar E,Abasiyanik M F,Bektik E,et al.Effect of heat processing on DNA quantification of meat species[J].Journal of Food Science,2012,77(9),40-44.
[25]Pereira L,Gomes S,Castro C,et al.High resolution melting(HRM)applied to wine authenticity[J].Food Chemistry,2017,216:80-86.
[26]Tomas C,I M P L V O Ferreira,Faria M A.Codfish authentication by a fast short amplicon high resolution melting analysis(SA-HRMA)method[J].Food Control,2017,71:255-263.
[27]Jaakola L,Suokas M,Hggman H.Novel approaches based on DNA barcoding and high-resolution melting of amplicons for authenticity analyses of berry species[J].Food Chemistry,2010,123(2):494-500.
[28]郭瑜,赖松家.线粒体DNA的研究及其应用[J].畜禽业,2004(11):64-66.
[29]闫华超.动物线粒体DNA分析技术及其应用[J].生物技术通讯,2010,21(2):280-283,294.
[30]Robin E D,Wong R.Mitochondrial DNA molecules and virtual number of mitochondria per cell in mammalian cells[J].Journal of Cellular Physiology,1988,136(3):507-513.
[31]Sarri C,Stamatis C,Sarafidou T,et al.A new set of 16S rRNA universal primers for identification of animal species[J].Food Control,2014,43(5):35-41.
[32]Lopez Oceja A,Gamarra D,Borragan S,et al.New cyt b gene universal primer set for forensic analysis[J].Forensic Science International Genetics,2016,23:159-165.
[33]何玮玲,张驰,杨静,等.食品中4种肉类成分多重PCR的快速鉴别方法[J].中国农业科学,2012,45(9):1 873-1 880.
[34]Mafra I,urea Roxo,I M P L V O Ferreira,et al.A duplex polymerase chain reaction for the quantitative detection of cows′milk in goats′milk cheese[J].International Dairy Journal,2007,17(9):1 132-1 138.
[35]田金辉.食品中动物源性成分定性鉴定方法研究[D].杨陵:西北农林科技大学,2011.
[36]Kitano T,Umetsu K,Tian W,et al.Two universal primer sets for species identification among vertebrates[J].International Journal of Legal Medicine,2007,121(5):423-427.
[37]宋洁,张小芳,刁雪淘,等.线粒体DNA12SrRNA、16SrRNA研究进展[J].安徽农学通报,2008,14(9):42-43.
[38]Taberlet P,Coissac E,Pompanon F,et al.Power and limitations of the chloroplast trnL(UAA)intron for plant DNA barcoding[J].Nucleic Acids Research,2007,35(3):1-8.
[39]付涛,王志龙,钱萍仙,等.高等植物DNA条形码最新研究进展及其应用[J].核农学报,2016,30(5):887-896.
[40]杨慧洁,杨世海,张淑丽,等.药用植物DNA条形码研究进展[J].中草药,2014,45(18):2 581-2 587.
[41]Sakaridis I,Ganopoulos I,Argiriou A,et al.A fast and accurate method for controlling the correct labeling of products containing buffalo meat using High Resolution Melting(HRM)analysis[J].Meat Science,2013,94(1):84-88.
[42]Klomtong P,Phasuk Y,Duangjinda M.Animal species identification through high resolution melting real time PCR(HRM)of the mitochondrial 16SrRNA gene[J].Annals of Animal Science,2016,16(2):415-424.
[43]Lopez Oceja A,Nu1ez C,Baeta M,et al.Species identification in meat products:A new screening method based on high resolution melting analysis of cyt b gene[J].Food Chemistry,2017,237:701-706.
[44]Naue J,Hansmann T,Schmidt U.High-resolution melting of 12SrRNA and cytochrome b DNA sequences for discrimination of species within distinct european animal families[J].Plos One,2014,9(12):1-17.
[45]Yang S,Li C,Wu Q,et al.High-resolution melting analysis:A promising molecular method for meat traceability[J].European Food Research&Technology,2014,239(3):473-480.
[46]Wu Q Y,Zhou G H,Yang S S,et al.SNP genotyping in sheep from northwest and east China for meat traceability[J].Journal of Consumer Protection&Food Safety,2017,12:1-6.
[47]Wu Q Y,Zhou G H,Yang S S,et al.Combined SNPs and miRNAs technologies for beef traceability[J].Journal of Food Safety,2017,37(4):1-11.
[48]Jilberto F,Araneda C,Larraín M A.High resolution melting analysis for identification of commercially-important Mytilus species[J].Food Chemistry,2017,229:716-720.
[49]Fernandes T J R,Costa J,Oliveira M B,et al.DNA barcoding coupled to HRM analysis as a new and simple tool for the authentication of Gadidae fish species[J].Food Chemistry,2017,230:49-57.
[50]Fernandes T J R,Costa J,Oliveira M B P P,et al.COI barcode-HRM as a novel approach for the discrimination of hake species[J].Fisheries Research,2017,197:50-59.
[51]Ganopoulos I,Sakaridis I,Argiriou A,et al.A novel closed-tube method based on high resolution melting(HRM)analysis for authenticity testing and quantitative detection in Greek PDO Feta cheese[J].Food Chemistry,2013,141(2):835-840.
[52]Sakaridis I,Ganopoulos I,Argiriou A,et al.High resolution melting analysis for quantitative detection of bovine milk in pure water buffalo mozzarella and other buffalo dairy products[J].International Dairy Journal,2013,28(1):32-35.
[53]Madesis P,Ganopoulos I,Anagnostis A,et al.The application of Bar-HRM(Barcode DNA-High Resolution Melting)analysis for authenticity testing and quantitative detection of bean crops(Leguminosae)without prior DNA purification[J].Food Control,2012,25(2):576-582.
[54]Ganopoulos I,Madesis P,Darzentas N,et al.Barcode High Resolution Melting(Bar-HRM)analysis for detection and quantification of PDO “Fava Santorinis”(Lathyrus clymenum)adulterants[J].Food Chemistry,2012,133(2):505-512.
[55]Ganopoulos I,Bosmali I,Madesis P,et al.Microsatellite genotyping with HRM(High Resolution Melting)analysis for identification of the PGI common bean variety plake megalosperma prespon[J].European Food Research&Technology,2012,234(3):501-508.
[56]Bosmali I,Ganopoulos I,Madesis P,et al.Microsatellite and DNA-barcode regions typing combined with High Resolution Melting(HRM)analysis for food forensic uses:A case study on lentils(Lens culinaris)[J].Food Research International,2012,46(1):141-147.
[57]Ganopoulos I,Argiriou A,Tsaftaris A.Adulterations in basmati rice detected quantitatively by combined use of microsatellite and fragrance typing with High Resolution Melting(HRM)analysis[J].Food Chemistry,2011,129(2):652-659.
[58]Ganopoulos I,Bazakos C,Madesis P,et al.Barcode DNA high-resolution melting(Bar-HRM)analysis as a novel close-tubed and accurate tool for olive oil forensic use[J].Journal of the Science of Food&Agriculture,2013,93(9):2 281-2 286.
[59]Vietina M,Agrimonti C,Marmiroli N.Detection of plant oil DNA using high resolution melting(HRM)post PCR analysis:A tool for disclosure of olive oil adulteration[J].Food Chemistry,2013,141(4):3 820-3 826.
[60]Montemurro C,Miazzi M M,Pasqualone A,et al.Traceability of PDO olive oil“Terra di Bari”using high resolution melting[J].Journal of Chemistry,2015,205(1):1-7.
[61]Distefano G,Caruso M,La M S,et al.High resolution melting analysis is a more sensitive and effective alternative to gel-based platforms in analysis of SSR-an example in citrus[J].Plos One,2012,7(8):1-11.
[62]Mackay J F,Wright C D,Bonfiglioli R G.A new approach to varietal identification in plants by microsatellite high resolution melting analysis:Application to the verification of grapevine and olive cultivars[J].Plant Methods,2008,4(1):1-10.
[63]张梦婷,孙伟,师玉华,等.基于HRM的常见伞形科香辛料快速鉴定[J].中国实验方剂学杂志,2018,24(6):26-31.
[64]Heejin J,Yeongdeuk J,Soungwoo P,et al.Identification of capsicum species using SNP markers based on high resolution melting analysis[J].Genome,2010,53(12):1 029-1 040.
[65]Liu Y,Xiang L,Zhang Y,et al.DNA barcoding based identification of hippophae species and authentication of commercial products by high resolution melting analysis[J].Food Chemistry 2018,242:62-67.
[66]Jiang C,Cao L,Yuan Y,et al.Barcoding melting curve analysis for rapid,sensitive,and discriminating authentication of saffron(Crocus sativus L.)from its adulterants[J].Biomed Research International,2014(1):1-10.
[67]Bosmali I,Ordoudi S A,Tsimidou M Z,et al.Greek PDO saffron authentication studies using species specific molecular markers[J].Food Research International 2017,100:899-907.
[68]王志科,鲁放,熊超,等.Bar-HRM技术在人参和西洋参药材鉴定中的应用研究[J].世界科学技术-中医药现代化,2016,18(2):191-195.
[69]陈媞颖,蒋超,袁媛,等.基于DNA熔解曲线技术的金银花种质鉴定[J].中国中药杂志,2016,41(24):4 572-4 578.
[70]熊超,李景剑,孙伟,等.HRM结合DNA条形码在苦杏仁对桃仁掺混检测中的应用[J].药学学报,2017,52(4):647-652.
[71]童宇茹,蒋超,黄璐琦,等.基于DNA熔解曲线分析技术的三七粉分子鉴定[J].药物分析杂志,2014,34(8):1 384-1 390.