摘要
目的建立藿香正气丸的薄层鉴别和含量测定方法。方法采用TLC法定性鉴别藿香正气丸中的广藿香、厚朴、陈皮、白芷、甘草及白术;采用HPLC法测定制剂中橙皮苷、厚朴酚及和厚朴酚的含量。结果藿香正气丸中广藿香、厚朴、陈皮、白芷、甘草及白术的TLC图谱斑点清晰、重复性好、专属性强、分离较好、方法简便。制剂中橙皮苷、和厚朴酚及厚朴酚的线性范围分别为0.95~9.46,0.32~3.16和0.89~8.90μg,水丸平均加样回收率分别为100.63%,101.41%和100.17%,RSD值分别为0.94%,1.23%和0.45%;浓缩丸平均加样回收率分别为100.87%,101.30%和100.40%,RSD值分别为1.28%,1.14%和1.22%;2个指标测定的精密度、重复性、稳定性和准确度RSD值均低于3.00%。结论该方法简便、快捷,结果准确、可靠,可作为该制剂的质量控制标准。
Objective To establish the methods of TLC identification and content determination for Huoxiang Zhengqi Pills.Methods Herba pogostemonis,Magnolia officinalis Cortex,Angelicae dahuricae Radix,Pericarpium citri Reticulatae,Glycyrrhizae Radix et Rhizoma and Rhizoma atractylodis Macrocephalae were identified by TLC.The contents of aurantiamarin,magnolol and honokiol were determined by HPLC.Results The TLC spots were clear and showed good repeatability.Aurantiamarin showed a linear relationship in the range of 0.95-9.46μg with an average recovery of 100.63%(RSD was 0.94%)in water pills and an average recovery of 100.87%(RSD was 1.28%)in concentrated pills;magnolol showed a linear relationship in the range of 0.32-3.16μg with an average recovery of 101.41%(RSD was 1.23%)in water pills and an average recovery of 101.30%(RSD was1.14%)in concentrated pills;honokiol showed a linear relationship in the range of 0.89-8.90μg with an average recovery of100.17%(RSD was 0.45%)in water pills and an average recovery of 100.40%(RSD was 1.22%)in concentrated pills.The RSDs of precision,reproducibility,stability and accuracy were all less than 3.00%.Conclusion The methods are easy,fast and reliable,and could be used as a quality control method for Huoxiang Zhengqi Pills.
引文
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