外泌体检测鉴定及其在凝血中作用的研究进展
摘要
外泌体是能够在细胞间传递信息的细胞分泌的纳米尺寸的膜结构颗粒。有效鉴定检测外泌体是外泌体研究领域的首要问题。除了透射电镜之外,近来新开发的诸如流式细胞术、纳米颗粒追踪分析或电阻脉冲传感等方法已被越来越多地运用到外泌体检测。明确这些方法的原理、适用性及进展无疑对研究外泌体具有重要意义。本文作者对这些方法检测外泌体最新进展进行综述,讨论外泌体参与凝血过程最新研究进展。
引文
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[31]KAPUSTIN A N,CHATROU M L,DROZDOV I,et al. Vascular smooth muscle cell calcification is mediated by regulated exosome secretion[J]. Cir Res,2015,116(8):1312-1323.
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[33] LOYER X,VION A-C,TEDGUI A,et al. Microvesicles as cell–cell messengers in cardiovascular diseases[J]. Cir Res,2014,114(2):345-353.
[34]GARDINER C,HARRISON P,BELTING M,et al. Extracellular vesicles,tissue factor,cancer and thrombosis–discussion themes of the isev 2014 educational day[J]. J Extracell Vesicles,2015,4(1):26901.
[35]MULDER A B,SMIT J,BOM V,et al. Association of smooth muscle cell tissue factor with caveolae[J]. Blood,1996,88(4):1306-1313.
[36]COSTALESS P,FUENTES-PRIOR P,CASTERLLANO J,et al. K domain cr9 of low density lipoprotein(ldl)receptor-related protein 1(lrp1)is critical for aggregated ldl-induced foam cell formation from human vascular smooth muscle cells[J]. J Biol Chem,2015,290(24):14852-14865.
[2] TKACH M,THERY C. Communication by extracellular vesicles:where we are and where we need to go[J]. Cell,2016,164(6):1226-1232.
[3]HESSVIK N P,LLORENTE A. Current knowledge on exosome biogenesis and release[J]. Cell Mol Life Sci,2018,75(2):193-208.
[4]MEDINA C B,RAVICHANDRNA K S. Do not let death do us part:'Find-me'signals in communication between dying cells and the phagocytes[J]. Cell Death Differ,2016,23(6):979-989.
[5]ZHANG C,JI Q,YANG Y,et al. Exosome:function and role in cancer metastasis and drug resistance[J/OL]. Technol Cancer Res Treat, 2018, 17:1533033818763450. DOI:10.1177/1533033818763450.
[6] SZATANEK R,BAJ-KRZYWORZEKA M,ZIMOCH J,et al.The methods of choice for extracellular vesicles(evs)characterization[J]. Int J Mol Sci,2017,18(6). DOI:10. 3390/ijms18061153.
[7]ERDBRUGGER U,LANNIGAN J. Analytical challenges of extracellular vesicle detection:a comparison of different techniques[J]. Cytometry A,2016,89(2):123-134.
[8]NOLAN J P,JONES J C. Detection of platelet vesicles by flow cytometry[J]. Platelets,2017,28(3):256-262.
[9]XU R,GREENING D W,ZHU H J,et al. Extracellular vesicle isolation and characterization:toward clinical application[J]. J Clin Invest,2016,126(4):1152-1162.
[10]LIBREGTS S,ARKESTEIJIN G J A,NEMETH A,et al. Flow cytometric analysis of extracellular vesicle subsets in plasma:impact of swarm by particles of non-interest[J/OL]. J Thromb Haemost,2018. DOI:10. 1111/jth. 14154.
[11]WELSH J A,HOLLOWAY J A,WIKINSON J S,et al. Extracellular vesicle flow cytometry analysis and standardization[J]. Front Cell Dev Biol,2017,5:78.
[12] ZWICKER J I,LACROIX R,DIGNAT-GEORGE F,et al.Measurement of platelet microparticles[J]. Methods Mol Biol,2012,788:127-139.
[13]SADEGHI J,PATABADIGE D E,CULBERTSON A H,et al.Out-of-plane integration of a multimode optical fiber for single particle/cell detection at multiple points on a microfluidic device with applications to particle/cell counting,velocimetry,size discrimination and the analysis of single cell lysate injections[J]. Lab Chip,2016,17(1):145-155.
[14]ZEMING K K,SALAFI T,SHIKHA S,et al. Fluorescent label-free quantitative detection of nano-sized bioparticles using a pillar array[J]. Nat Commun,2018,9(1):1254.
[15] COSCIA C,PAROLINI I,SANCHEZ M,et al. Generation,quantification,and tracing of metabolically labeled fluorescent exosomes[J]. Methods Mol Biol,2016,1448:217-235.
[16]ZHANG X F,LIU Z G,SHEN W,et al. Silver nanoparticles:synthesis,characterization,properties,applications,and therapeutic approaches[J/OL]. Int J Mol Sci,2016,17(9). DOI:10. 3390/ijms17091534.
[17]TUORINIEMI J,JOHNSSON A J,HOLMBERG J P,et al. Intermethod comparison of the particle size distributions of colloidal silica nanoparticles[J/OL]. Sci Technol Adv Mater,2014,15(3):035009. DOI:10. 1088/1468-6996/15/3/035009.
[18]WATANABE A,TAKAGI M,MURATA S,et al. Stability and drug release studies of an antimycotic nanomedicine using hplc,dynamic light scattering and atomic force microscopy[J]. J Pharm Biomed Anal,2018,148:149-155.
[19] RACZAT T,KRAEMER L,GALL C,et al. The influence of four different anticoagulants on dynamic light scattering of platelets[J]. Vox Sang,2014,107(2):196-199.
[20]VOGEL R,COUMANS F A,MALTESEN R G,et al. A standardized method to determine the concentration of extracellular vesicles using tunable resistive pulse sensing[J]. J Extracell Vesicles,2016,5:31242.
[21]SIKORA A,SHARD A G,MINELLI C. Size and zeta-potential measurement of silica nanoparticles in serum using tunable resistive pulse sensing[J]. Langmuir,2016,32(9):2216-2224.
[22]WILLMOTT G R. Tunable resistive pulse sensing:better size and charge measurements for submicrometer colloids[J]. Anal Chem,2018,90(5):2987-2995.
[23]VARGA Z,YUANA Y,GROOTEMAAT A E,et al. Towards traceable size determination of extracellular vesicles[J/OL].J Extracell Vesicles,2014,3. DOI:10. 3402/jev. v3. 23298.
[24]CARNELL-MORRIS P,TANNETTA D,SIUPA A,et al. Analysis of extracellular vesicles using fluorescence nanoparticle tracking analysis[J]. Methods Mol Biol,2017,1660:153-173.
[25]PARSONS M E M,MCPARLAND D,SZKLANNA P B,et al.A protocol for improved precision and increased confidence in nanoparticle tracking analysis concentration measurements between 50 and 120 nm in biological fluids[J]. Front Cardiovasc Med,2017,4:68.
[26]HUBER H J,HOLVOET P. Exosomes:emerging roles in communication between blood cells and vascular tissues during atherosclerosis[J]. Curr Opin Lipidol,2015,26(5):412-419.
[27]HEIJNEN H F,SCHIEL A E,FIJNHEER R,et al. Activated platelets release two types of membrane vesicles:microvesicles by surface shedding and exosomes derived from exocytosis of multivesicular bodies and alpha-granules[J]. Blood,1999,94(11):3791-3799.
[28]GIDLOFO,van der BRUG M,OHMAN J,et al. Platelets activated during myocardial infarction release functional mirna,which can be taken up by endothelial cells and regulate icam1 expression[J]. Blood,2013,121(19):3908-3917,S3901-3926.
[29]SRIKANTHAN S,LI W,SILVERSTEIN R L,et al. Exosome poly-ubiquitin inhibits platelet activation,downregulates cd36and inhibits pro-atherothombotic cellular functions[J]. J Thromb Haemost,2014,12(11):1906-1917.
[30]GASECKA A,NIEUWLAND R,van der POL E,et al. P2y12antagonist ticagrelor inhibits the release of procoagulant extracellular vesicles from activated platelets:preliminary results[J/OL]. Cardiol J,2018. DOI:10. 5603/CJ. a2018. 0045.
[31]KAPUSTIN A N,CHATROU M L,DROZDOV I,et al. Vascular smooth muscle cell calcification is mediated by regulated exosome secretion[J]. Cir Res,2015,116(8):1312-1323.
[32]NOMURA S,SHIMIZU M. Clinical significance of procoagulant microparticles[J]. J Intensive Care,2015,3(1):2.
[33] LOYER X,VION A-C,TEDGUI A,et al. Microvesicles as cell–cell messengers in cardiovascular diseases[J]. Cir Res,2014,114(2):345-353.
[34]GARDINER C,HARRISON P,BELTING M,et al. Extracellular vesicles,tissue factor,cancer and thrombosis–discussion themes of the isev 2014 educational day[J]. J Extracell Vesicles,2015,4(1):26901.
[35]MULDER A B,SMIT J,BOM V,et al. Association of smooth muscle cell tissue factor with caveolae[J]. Blood,1996,88(4):1306-1313.
[36]COSTALESS P,FUENTES-PRIOR P,CASTERLLANO J,et al. K domain cr9 of low density lipoprotein(ldl)receptor-related protein 1(lrp1)is critical for aggregated ldl-induced foam cell formation from human vascular smooth muscle cells[J]. J Biol Chem,2015,290(24):14852-14865.