昆虫病原线虫与吡虫啉混用对异迟眼蕈蚊幼虫的毒力测定
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摘要
【目的】为了解决生产中韭菜异迟眼蕈蚊(Bradysia difformis Frey)防治难、农药残留超标等问题,将昆虫病原线虫与高效低毒的化学杀虫剂减量混用,是一种高效环保的防控手段.【方法】以甘肃省分离得到的优良昆虫病原线虫Steinernema feltiae 0619HT品系,高效低毒农药吡虫啉为研究材料,采用药膜法测定了吡虫啉原药单用、与昆虫病原线虫斯氏线虫Steinernema feltiae 0619HT品系混配,对韭菜异迟眼蕈蚊幼虫的室内毒力,并计算毒力回归方程及致死中浓度LC_(50),以评价线虫与吡虫啉混用对韭菜异迟眼蕈蚊的控制效能.【结果】昆虫病原线虫Steinernema feltiae 0619HT品系,以1 000 Ijs/mL与低剂量10 mg/L的吡虫啉混配,韭菜异迟眼蕈蚊幼虫的校正死亡率达73.3%,比单用吡虫啉(校正死亡率为43.3%)的毒力更高(P<0.05).【结论】昆虫病原线虫与低剂量吡虫啉混用,对异迟眼蕈蚊的毒力增强,具有明显的增效作用,可起到吡虫啉减量增效的效果.
【Objective】 To find out the environment-friendly biocontrol application to control Bradysia difformis which damages leek by using the mixture of entomopathogenic nematode(EPN) and low dose of low toxicity pesticides.【Method】 The control efficiency of mixture of an native EPN Steinernema feltiae 0619 HT in Gansu and imidacloprid against B.difformis was tested with insecticide-impregnated filter method,and the virulence regression equation and LC_(50) value were obtained.【Result】 The mortality rate for larvae of B.difformis was 73.3% under the condition of 1000 IJs/mL S.feltiae 0619 HT and 10 mg/L of imidacloprid,and it was 43.3% while only imidacloprid was used.【Conclusion】 The control efficiency of imidacloprid against B.difformis larvae could be enhanced by mixing EPN and EPN could reduce the application amount of imidacloprid.
【Objective】 To find out the environment-friendly biocontrol application to control Bradysia difformis which damages leek by using the mixture of entomopathogenic nematode(EPN) and low dose of low toxicity pesticides.【Method】 The control efficiency of mixture of an native EPN Steinernema feltiae 0619 HT in Gansu and imidacloprid against B.difformis was tested with insecticide-impregnated filter method,and the virulence regression equation and LC_(50) value were obtained.【Result】 The mortality rate for larvae of B.difformis was 73.3% under the condition of 1000 IJs/mL S.feltiae 0619 HT and 10 mg/L of imidacloprid,and it was 43.3% while only imidacloprid was used.【Conclusion】 The control efficiency of imidacloprid against B.difformis larvae could be enhanced by mixing EPN and EPN could reduce the application amount of imidacloprid.
引文
[1] 张艳霞,郭苏帆,刘长仲.异迟眼蕈蚊在不同植物上的生长发育及种群参数[J].应用昆虫学报,2016,53(6):1184-1189.
[2] 苟玉萍,刘倩,张艳霞,等.不同韭菜品种对异迟眼蕈蚊生长发育及繁殖的影响[J].中国生态农业学报,2015,23(6):741-747.
[3] 史彩华,胡静荣,李传仁,等.采用两种不同施药方法评价8种药剂对韭蛆的防治效果[J].应用昆虫学报,2016,53(6):1225-1232.
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[6] 王玉东,肖春,尹姣,等.三种化学杀虫剂对病原线虫侵染暗黑鳃金龟能力的影响[J].中国生物防治学报,2012,28(1):67-73.
[7] 孙瑞红,李爱华.昆虫病原线虫H06与化学杀虫剂对韭菜迟眼蕈蚊的联合作用[J].农药学学报,2007,9(1):66-70.
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[9] 刘其全,邱良妙,吴玮,等.11种稻田常用杀虫剂对拟环纹豹蛛的室内安全性评价[J].福建农业学报,2016,30 (11):1226-1230.
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[12] 魏洪义,王国汉,庞雄飞.几种杀虫剂对斯氏线虫的毒力[J].昆虫天敌,1991,13(2):91-93.
[13] 王志超,王思一,史雪岩,等.吡虫啉与三种有机磷杀虫剂混配对韭菜迟眼蕈蚊幼虫的室内毒力测定[J].2014,41(4):511-512.
[14] 秦久刚.新线虫对药剂敏感性的测定[J].昆虫天敌,1984,6(1):46-48.
[15] Koppenh?fer A M,Cowles R S,Cowles E A,et al.Comparison of neonicotinoid insecticides as synergists for entomopathogenic nematodes[J].Biological Control,2002,24:90-97.
[16] 王果红,韩日畴,陈镜华,等.利用昆虫病原线虫与化学农药混用防治褐纹甘蔗象[J].中国生物防治,2007,23(3):218-222.
[17] 张中润,曹莉,刘秀玲,等.昆虫病原线虫Steinernema longicaudum X-7增效药剂的筛选[J].昆虫知识,2005,43(1):68-73.
[18] Koppenh¨ofer A M,Brown I M,Gaugler R,et al.Synergists of entomopathogenic nematodes and imi-dacloprid against white grubs:greenhouse and field evaluation[J].Biological Control,2000,19:245 -251.
[19] Koppenh ofer A M,Kaya H K.Synergism of imidacloprid and an entomopathogenic nematode:a novel approach to white grub (Coleoptera:Scarabaeidae) control in turfgrass[J].Journal of Economic Entomology,1998,9(1):618-623.
[20] 杨秀芬,简恒,杨怀文,等.用昆虫病原线虫防治韭菜蛆[J].植物保护学报,2004,31(1):33-37.
[21] 雷仲仁,吴圣勇,王海鸿.我国蔬菜害虫生物防治研究进展[J].植物保护,2016,42,(1):1-6.
[2] 苟玉萍,刘倩,张艳霞,等.不同韭菜品种对异迟眼蕈蚊生长发育及繁殖的影响[J].中国生态农业学报,2015,23(6):741-747.
[3] 史彩华,胡静荣,李传仁,等.采用两种不同施药方法评价8种药剂对韭蛆的防治效果[J].应用昆虫学报,2016,53(6):1225-1232.
[4] 钱秀娟,谷黎娜,邢玉芳,等.甘肃省昆虫病原线虫区系研究[J].草地学报,2014,22(3):593-599.
[5] 张中润,韩日畴,许再福.草坪地下害虫蛴螬的生物防治研究进展[J].中国生物防治,2004,41(5):387-392.
[6] 王玉东,肖春,尹姣,等.三种化学杀虫剂对病原线虫侵染暗黑鳃金龟能力的影响[J].中国生物防治学报,2012,28(1):67-73.
[7] 孙瑞红,李爱华.昆虫病原线虫H06与化学杀虫剂对韭菜迟眼蕈蚊的联合作用[J].农药学学报,2007,9(1):66-70.
[8] 钱秀娟,刘长仲,阮艳娥.昆虫病原线虫对甘肃省草地蛴螬的致病力研究[J].草地学报,2015,23(2):414-421.
[9] 刘其全,邱良妙,吴玮,等.11种稻田常用杀虫剂对拟环纹豹蛛的室内安全性评价[J].福建农业学报,2016,30 (11):1226-1230.
[10] 中华人民共和国农业部.无公害食品韭菜生产技术规程 NY/T5002-2001[S].2001.
[11] 武海斌,辛力,宫庆涛,等.化学杀虫剂对昆虫病原线虫侵染韭菜迟眼蕈蚊能力的影响[J].应用昆虫学报,2014,51(4):1060-1068.
[12] 魏洪义,王国汉,庞雄飞.几种杀虫剂对斯氏线虫的毒力[J].昆虫天敌,1991,13(2):91-93.
[13] 王志超,王思一,史雪岩,等.吡虫啉与三种有机磷杀虫剂混配对韭菜迟眼蕈蚊幼虫的室内毒力测定[J].2014,41(4):511-512.
[14] 秦久刚.新线虫对药剂敏感性的测定[J].昆虫天敌,1984,6(1):46-48.
[15] Koppenh?fer A M,Cowles R S,Cowles E A,et al.Comparison of neonicotinoid insecticides as synergists for entomopathogenic nematodes[J].Biological Control,2002,24:90-97.
[16] 王果红,韩日畴,陈镜华,等.利用昆虫病原线虫与化学农药混用防治褐纹甘蔗象[J].中国生物防治,2007,23(3):218-222.
[17] 张中润,曹莉,刘秀玲,等.昆虫病原线虫Steinernema longicaudum X-7增效药剂的筛选[J].昆虫知识,2005,43(1):68-73.
[18] Koppenh¨ofer A M,Brown I M,Gaugler R,et al.Synergists of entomopathogenic nematodes and imi-dacloprid against white grubs:greenhouse and field evaluation[J].Biological Control,2000,19:245 -251.
[19] Koppenh ofer A M,Kaya H K.Synergism of imidacloprid and an entomopathogenic nematode:a novel approach to white grub (Coleoptera:Scarabaeidae) control in turfgrass[J].Journal of Economic Entomology,1998,9(1):618-623.
[20] 杨秀芬,简恒,杨怀文,等.用昆虫病原线虫防治韭菜蛆[J].植物保护学报,2004,31(1):33-37.
[21] 雷仲仁,吴圣勇,王海鸿.我国蔬菜害虫生物防治研究进展[J].植物保护,2016,42,(1):1-6.