摘要
为探究硒化大蒜多糖对环磷酰胺所致免疫抑制的颉颃作用,将336只11日龄非免疫健康罗曼雏鸡随机均分为7组:空白对照组(BC)、免疫对照组(VC)、环磷酰胺对照组(Cy)、sGPS3、sGPS5、sGPS6和药物对照组(APS),每组6个重复,每个重复8只鸡。BC组和VC组注射0.5mL生理盐水,其余组均肌肉注射8mg/mL环磷酰胺0.5mL,每天1次,连续3d;14日龄时,除BC组外,其余组均用新城疫疫苗免疫,同时3个硒大蒜化多糖组分别注射0.5mL浓度为1mg/mL的sGPS3、sGPS5、sGPS6,APS组肌肉注射黄芪多糖注射液0.5mL,Cy组、VC组和BC组注射生理盐水0.5mL,每天1次,连续3d,28日龄二免。分别于首免后的第7、14、21、28天,每组随机抽取6只罗曼鸡翼静脉采血,分离血清,β-微量法检测血清ND-HI抗体效价、ELISA法检测血清γ干扰素(IFN-γ)和白细胞介素2(IL-2)含量。称量体重后采集胸腺、脾脏和法氏囊组织并称重,计算免疫器官指数。结果显示,各给药组的血清ND-HI抗体效价、血清IFN-γ和IL-2含量、法氏囊指数均显著高于Cy组(P<0.05);sGPS6组血清抗体效价、IFN-γ含量在28d,血清IL-2含量在21、28d均显著高于其余各组(P<0.05);在免疫后21、28d,4个给药组中sGPS6组体重、法氏囊指数最高,显著高于其余3个给药组(P<0.05);在免疫后28d,4个给药组中sGPS6组胸腺指数、脾脏指数最高,显著高于其余3个给药组(P<0.05)。结果表明,硒化大蒜多糖对环磷酰胺所致的免疫抑制具有颉颃作用,其中sGPS6组的活性最高,可作为新型免疫抑制颉颃剂的候选药。
In order to observe the antagonistic effect of selenizing garlic polysaccharide on immunosuppressed chicken induced by cyclophosphamide,336 11-day-old roman chickens were randomly divided into seven groups:Blank control group(BC),vaccine group(VC),cyclophosphamidegroup(Cy),sGPS3 group,sGPS5 group,sGPS6 group and drug control group(APS),with 6 duplications per group and 8 chickens per duplication.Each chicken except that in BC and VC groups were intramuscularly injected with 8 mg/mL cyclophosphamide 0.5 mL,while each chicken in BC and VC groups were injected with the same amount of saline,once a day for three successive days.At the 14 dof age,The chickens except BC group were vaccinated with ND vaccine,repeated vaccination at 28 days old.At the first vaccination,the chickens in three sGPSs groups were injected with 0.5 mL of 1 mg/mL sGPS3,sGPS5 and sGPS6,respectively.The chickens in APS group were intramuscularly injected with 0.5 mL Astragalus polysaccharide,while that in Cy,VC and BC groups were injected with the same amount of saline,once a day for three successive days.Six chickens were selected randomly from each group for collecting wing venous blood on 7,14,21 and 28 days after the first vaccination,and the serum ND-HI antibody titer were determined byβ-micro method,IFN-γand IL-2 contents were determined by ELISA.The body weighed,thymus,spleen,bursa of Fabricius were collected after culling,and the immune organ indexes were calculated.The result showed that the serum ND-HI antibody titer,IFN-γand IL-2 contents and bursa of Fabricius index of each administration groups were higher than that of the Cy group(P<0.05);the serum ND-HI antibody titer and IFN-γcontents at 21 d,IL-2 content at 21 and 28 din sGPS6 group were significantly higher than that of other groups(P<0.05);At 21 and 28 dafter first vaccination,the body weight and bursa of Fabricius index in sGPS6 group were significantly higher than that of other three administration groups(P<0.05).At 28 dafter first vaccination,the thymus and spleen indexes in sGPS6 group were significantly higher than that of other three administration groups(P<0.05).In conlusion,the selenizing garlic polysaccharide had the antagonistic effect on immunosuppressed chicken induced by cyclophosphamide,and sGPS6 had the better effect which could be used as candidate drug for novel immunosuppressive antagonists.
引文
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