生脉姜黄散对鼻咽癌放射抗拒株细胞移植瘤裸鼠肠道菌群的影响
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摘要
现代药理研究表明,生脉散具有增强免疫力、改善血液循环等功效;姜黄具有抗炎、抗癌、抗氧化等多种作用。生脉散联合姜黄在中药抗肿瘤的研究中是一种崭新的研究方向。目前鼻咽癌的主要治疗手段是放射治疗,但众所周知放射治疗会引起多种副作用,同时也会改变肠道菌群的构成。研究表明,鼻咽癌放疗后机体的病机主要为正虚血瘀,生脉姜黄散体现了中医益气活血养阴的重要治法。该研究用鼻咽癌抗照射细胞株对裸鼠种植移植瘤,然后利用16s rDNA测序平台对服用生脉姜黄散或照射治疗裸鼠的肠道粪便样本进行宏基因测序,之后对肠道菌群数据结果进行分析,探讨生脉姜黄散对肿瘤移植裸鼠肠道菌群结构产生的影响。结果表明,生脉姜黄散联合照射治疗可增强肿瘤治疗的疗效,放射治疗会降低裸鼠肠道菌群的总数与多样性,同时也会改变菌群结构组成。生脉姜黄散可保护菌落的多样性,也能部分恢复因照射引起的菌落失调。该研究为生脉姜黄散作为鼻咽癌放射治疗的增敏辅助用药提供了研究思路。
Modern pharmacological studies have shown that Shengmai San has the effects of enhancing immunity and improving blood circulation, and Curcumae Longae Rhizoma(Jianghuang) has anti-inflammatory, anti-cancer, anti-oxidation and other functions. Shengmai San combined with Jianghuang is a new research direction in the study of anti-tumor of traditional Chinese medicines. The main treatment for nasopharyngeal carcinoma is radiation therapy, but radiation therapy can cause a variety of side effects, and it also changes the composition of the intestinal flora. In this study, the 16 s rDNA sequencing platform was used to perform macro-sequence sequencing of the intestinal flora samples of nude mice bearing the veins of Shengmai Jianghuang San, and then the results of intestinal flora data were analyzed to investigate the effect of Shengmai Jianghuang San on tumors. The results showed that Shengmai Jianghuang San combined with irradiation could enhance the therapeutic effect of tumor treatment. Radiation therapy would reduce the total number and diversity of intestinal flora in nude mice, and also change the structure of the flora. Shengmai Jianghuang San could protect the diversity of colonies, and also partially restore the colony imbalance caused by irradiation. This study provides a research idea for Shengmai Jianghuang San as a sensitizing adjuvant for radiotherapy of nasopharyngeal carcinoma.
Modern pharmacological studies have shown that Shengmai San has the effects of enhancing immunity and improving blood circulation, and Curcumae Longae Rhizoma(Jianghuang) has anti-inflammatory, anti-cancer, anti-oxidation and other functions. Shengmai San combined with Jianghuang is a new research direction in the study of anti-tumor of traditional Chinese medicines. The main treatment for nasopharyngeal carcinoma is radiation therapy, but radiation therapy can cause a variety of side effects, and it also changes the composition of the intestinal flora. In this study, the 16 s rDNA sequencing platform was used to perform macro-sequence sequencing of the intestinal flora samples of nude mice bearing the veins of Shengmai Jianghuang San, and then the results of intestinal flora data were analyzed to investigate the effect of Shengmai Jianghuang San on tumors. The results showed that Shengmai Jianghuang San combined with irradiation could enhance the therapeutic effect of tumor treatment. Radiation therapy would reduce the total number and diversity of intestinal flora in nude mice, and also change the structure of the flora. Shengmai Jianghuang San could protect the diversity of colonies, and also partially restore the colony imbalance caused by irradiation. This study provides a research idea for Shengmai Jianghuang San as a sensitizing adjuvant for radiotherapy of nasopharyngeal carcinoma.
引文
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[20] 李平兰,时向东,吕燕妮,等. 常见中草药对两种肠道有益菌体外生长的影响[J]. 中国农业大学学报,2003(5):33.
[21] 王广. 党参多糖对肠道菌群失调小鼠的调整作用机制的初步研究[D]. 佳木斯:佳木斯大学,2010.
[22] 花海莹,李雪晴,刘吉华. 生脉散对人肠道菌群失衡的调节作用[J]. 中国药科大学学报,2016,47(1):95.
[23] 孙艺凡,张霞,王晓艳,等. 长期服用人参提取物对大鼠肠道菌群结构的影响[J]. 中国中药杂志,2018,43(19):3927.
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[26] 郑勇,郑袁明,张丽梅,等. 极端环境下嗜热酸甲烷营养细菌研究进展[J]. 生态学报,2009,29(7):3864.
[2] Sze H, Blanchard P, Ng W T, et al. Chemotherapy for nasopharyngeal carcinoma--current recommendation and controversies[J]. Hematol Oncol Clin North Am,2015,29(6):1107.
[3] Chen W, Hu G H. Biomarkers for enhancing the radiosensitivity of nasopharyngeal carcinoma[J]. Cancer Biol Med,2015,12(1):23.
[4] 丘文泽,黄培钰,施君理,等. 调强放疗结合诱导化疗或同期加辅助化疗治疗局部晚期鼻咽癌的疗效比较[J]. 中国肿瘤临床,2015,42(4):231.
[5] Crawford P A, Gordon J I. Microbial regulation of intestinal radiosensitivity[J]. Proc Natl Acad Sci USA,2005,102(37):13254.
[6] Wang Q, Fan H, Liu Y, et al. Curcumin enhances the radiosensitivity in nasopharyngeal carcinoma cells involving the reversal of differentially expressed long non-coding RNAs[J]. Int J Oncol,2014,44(3):858.
[7] 魏辉,王启瑞,刘英,等. 加味生脉散对气虚型鼻咽癌大鼠的放射增敏作用及相关机制[J]. 中国实验方剂学杂志,2012,18(24):233.
[8] 黄沐,李爱武,刘钊汝,等. 生脉姜黄散对鼻咽癌CNE-2细胞裸鼠移植瘤的放射增敏作用[J]. 中国实验方剂学杂志,2018,24(6):145.
[9] 朱道琦,黄沐,刘钊汝,等. 姜黄素对鼻咽癌抗拒株的放射增敏作用及其机制研究[J]. 中国药理学通报,2017,33(8):1086.
[10] 刘丽,张石川. 铂类在鼻咽癌同步放化疗中的应用[J]. 肿瘤预防与治疗,2017,30(2):136.
[11] 江曼,钱晓萍,刘宝瑞. 恶性肿瘤放射增敏剂研究进展[J]. 现代肿瘤医学,2014,22(1):226.
[12] 冉晨曦,何人可,汤小玲,等. 肿瘤放射治疗中辐射增敏剂的应用进展[J]. 山东医药,2015,55(3):86.
[13] 陈晓慧,李巍,鱼芳,等. 肺癌患者铂类药物化疗后肠道菌群变化的临床研究[J]. 中国微生态学杂志,2016,28(7):781.
[14] Veeraraghavan J, Aravindan S, Natarajan M, et al. Neem leaf extract induces radiosensitization in human neuroblastoma xenograft through modulation of apoptotic pathway[J]. Anticancer Res,2011,31(1):161.
[15] Jia L, Ma S, Hou X, et al. The synergistic effects of traditional Chinese herbs and radiotherapy for cancer treatment[J]. Oncol Lett,2013,5(5):1439.
[16] Thyagarajan A, Jedinak A, Nguyen H, et al. Triterpenes from Ganoderma lucidum induce autophagy in colon cancer through the inhibition of p38 mitogen-activated kinase (p38 MAPK)[J]. Nutr Cancer,2010,62(5):630.
[17] 姜东京,张丽,曹雨诞,等. 肠道菌群在中药研究中的应用[J]. 中国中药杂志,2016,41(17):3218.
[18] 安婉丽,李雪丽,孔冉,等.中医药治疗肠道菌群失调症的方剂用药规律分析[J].中国实验方剂学杂志,2018,24(12):210.
[19] 于岚,邢志凯,米双利,等. 中药对肠道菌群的调节作用[J]. 中国中药杂志,2019,44(1):34.
[20] 李平兰,时向东,吕燕妮,等. 常见中草药对两种肠道有益菌体外生长的影响[J]. 中国农业大学学报,2003(5):33.
[21] 王广. 党参多糖对肠道菌群失调小鼠的调整作用机制的初步研究[D]. 佳木斯:佳木斯大学,2010.
[22] 花海莹,李雪晴,刘吉华. 生脉散对人肠道菌群失衡的调节作用[J]. 中国药科大学学报,2016,47(1):95.
[23] 孙艺凡,张霞,王晓艳,等. 长期服用人参提取物对大鼠肠道菌群结构的影响[J]. 中国中药杂志,2018,43(19):3927.
[24] Palm N W, de Zoete M R, Cullen T W, et al. Immunoglobulin A coating identifies colitogenic bacteria in inflammatory bowel disease[J]. Cell,2014,158(5):1000.
[25] Latham E A, Weldon K K, Wickersham T A, et al. Responses in the rumen microbiome of Bos taurus and indicus steers fed a low-quality rice straw diet and supplemented protein[J]. J Anim Sci,2018,96(3):1032.
[26] 郑勇,郑袁明,张丽梅,等. 极端环境下嗜热酸甲烷营养细菌研究进展[J]. 生态学报,2009,29(7):3864.