响应面法优化炮天雄多糖超声提取工艺
|
摘要
目的优化炮天雄中多糖超声辅助提取的最佳工艺条件。方法以多糖得率为指标,在单因素实验的基础上,选择超声功率、超声时间和液料比3个提取因素为影响因素,根据Box-Behnken响应面实验原理设计3因素3水平的响应面实验,通过对各因子显著性和交互作用的分析,确定超声提取炮天雄多糖的最佳工艺。结果最佳提取工艺为超声功率270 W,超声时间13min,液料比25∶1(mL∶g),在此条件下,多糖得率为45.91%,与理论值46.14%非常接近。结论采用响应面法优化炮天雄多糖超声提取的工艺可行,可作为炮天雄多糖的提取方法。
Objective To optimize the ultrasonic-assisted extraction process of polysaccharides from Processed Tianxiong by response surface methodology.Methods 3 influencing factors(ultrasonic-power,ultrasonic-time,liquid-solid ratio)were selected to investigate polysaccharides yield which were based on single factor experiment,then the response surface test of 3 factors and 3 levels was designed,according to the theory of Box-Behnken response surface methodology.The ultrasonic-assisted extraction for polysaccharides from Processed Tianxiong was optimized by analyzing the significance and interaction of each factor.Results The optimal process extraction was as follows:the power of ultrasonics was 270 Wand the extraction time of ultrasonics was 13 min,and liquid-solid ratio was 25∶1(mL∶g).Under such conditions,the extraction ratio of polysaccharides from Processed Tianxiong was45.91%,which was basically closed to the predicted of 46.14%.Conclusion The extraction process of polysaccharides from Processed Tianxiong is feasible,and can be used for the extraction of polysaccharides from Processed Tianxiong.
Objective To optimize the ultrasonic-assisted extraction process of polysaccharides from Processed Tianxiong by response surface methodology.Methods 3 influencing factors(ultrasonic-power,ultrasonic-time,liquid-solid ratio)were selected to investigate polysaccharides yield which were based on single factor experiment,then the response surface test of 3 factors and 3 levels was designed,according to the theory of Box-Behnken response surface methodology.The ultrasonic-assisted extraction for polysaccharides from Processed Tianxiong was optimized by analyzing the significance and interaction of each factor.Results The optimal process extraction was as follows:the power of ultrasonics was 270 Wand the extraction time of ultrasonics was 13 min,and liquid-solid ratio was 25∶1(mL∶g).Under such conditions,the extraction ratio of polysaccharides from Processed Tianxiong was45.91%,which was basically closed to the predicted of 46.14%.Conclusion The extraction process of polysaccharides from Processed Tianxiong is feasible,and can be used for the extraction of polysaccharides from Processed Tianxiong.
引文
[1]曹晖,王春燕,王孝涛.毒性中药天雄炮制历史沿革研究[J].中国中药杂志,1998,23(6):348-350.
[2]国家药典委员会.中国药典:2015年版:一部[M].北京:中国医药科技出版社,2015:192.
[3]覃军,龚又明,郑显辉,等.岭南特色炮天雄和传统炮附片的成分比较[J].新中医,2016,48(12):220-223.
[4]曹晖,王时桃,吴连英,等.香港市售天雄补肾助阳药效学研究[J].中国中药杂志,2001,26(6):369-372.
[5]覃军,邓广海,陈艳红,等.响应面法优化岭南炮天雄生物碱提取工艺[J].中国医药导报,2017,14(19):31-34.
[6]高静东,李湧健,陈嘉璐,等.复方附子多糖对肿瘤细胞糖基化作用的影响[J].中国中西医结合杂志,2016,36(9):1103-1106.
[7]Zhang A Q,Li X Q,Xing C,et al.Antioxidant activity of polysaccharide extracted from Pleurotus eryngii using response surface methodology[J].Int J Bio Macromol,2014,65:28-32.
[8]Wang Z J,Xie J H,Nie S P,et al.Review on cell models to evaluate the potential antioxidant activity of polysaccharides[J].Food Funct,2017,8(3):915-926.
[9]李萍,李卫燕,曹蔚.酶解技术在多糖结构和活性研究中的应用进展[J].西北药学杂志,2015,30(4):433-436.
[10]陈文良,王冬梅,卢传礼,等.酶法提取土茯苓多糖的工艺研究[J].中药新药与临床药理,2015,26(3):392-396.
[11]张东东,许欢,刘银环,等.星点设计-响应面法优选鹿药总黄酮提取工艺[J].西北药学杂志,2015,30(5):556-558.
[12]Ghasemzadeh A,Jaafar H Z,Karimi E,et al.Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf(Murraya koenigii L.)using response surface methodology[J].BMC Complement Altern Med,2014,14:318.
[13]Ghasemzadeh A,Jaafar H Z,Rahmat A,et al.Optimization of microwave-assisted extraction of zerumbone from Zingiber zerumbet L.rhizome and evaluation of antiproliferative activity of optimized extracts[J].Chem Cent J,2017,11:5.
[14]刘瑞林,余佩,陈国宁,等.响应曲面法优化超声-微波协同提取澳洲坚果油的工艺研究[J].西北药学杂志,2017,32(6):718-722.
[15]蒋丽芸,黄玉梅,吴志坚,等.岭南炮天雄的炮制工艺优选[J].中国实验方剂学杂志,2015,21(21):24-27.
[16]张雪,宋玉琴,杨雨婷,等.附子炮制品水煎液对比格犬心脏急性毒性研究[J].中国民族民间医药,2015,24(16):21-23.
[17]宋玉琴,张雪,董艳红,等.附子不同炮制品灌胃Beagle犬的急性毒性研究[J].世界科学技术-中医药现代化,2015,17(7):1432-1437.
[18]覃军,邓广海,陈艳红,等.不同产地炮天雄的质量考察与比较[J].中国药业,2017,26(3):11-14.
[19]Wang M,Yang X B,Zhao J W,et al.Structural characterization and macrophage immunomodulatory activity of a novel polysaccharide from Smilax glabra Roxb[J].Carbohydrate Polym,2017,156:390-402.
[2]国家药典委员会.中国药典:2015年版:一部[M].北京:中国医药科技出版社,2015:192.
[3]覃军,龚又明,郑显辉,等.岭南特色炮天雄和传统炮附片的成分比较[J].新中医,2016,48(12):220-223.
[4]曹晖,王时桃,吴连英,等.香港市售天雄补肾助阳药效学研究[J].中国中药杂志,2001,26(6):369-372.
[5]覃军,邓广海,陈艳红,等.响应面法优化岭南炮天雄生物碱提取工艺[J].中国医药导报,2017,14(19):31-34.
[6]高静东,李湧健,陈嘉璐,等.复方附子多糖对肿瘤细胞糖基化作用的影响[J].中国中西医结合杂志,2016,36(9):1103-1106.
[7]Zhang A Q,Li X Q,Xing C,et al.Antioxidant activity of polysaccharide extracted from Pleurotus eryngii using response surface methodology[J].Int J Bio Macromol,2014,65:28-32.
[8]Wang Z J,Xie J H,Nie S P,et al.Review on cell models to evaluate the potential antioxidant activity of polysaccharides[J].Food Funct,2017,8(3):915-926.
[9]李萍,李卫燕,曹蔚.酶解技术在多糖结构和活性研究中的应用进展[J].西北药学杂志,2015,30(4):433-436.
[10]陈文良,王冬梅,卢传礼,等.酶法提取土茯苓多糖的工艺研究[J].中药新药与临床药理,2015,26(3):392-396.
[11]张东东,许欢,刘银环,等.星点设计-响应面法优选鹿药总黄酮提取工艺[J].西北药学杂志,2015,30(5):556-558.
[12]Ghasemzadeh A,Jaafar H Z,Karimi E,et al.Optimization of ultrasound-assisted extraction of flavonoid compounds and their pharmaceutical activity from curry leaf(Murraya koenigii L.)using response surface methodology[J].BMC Complement Altern Med,2014,14:318.
[13]Ghasemzadeh A,Jaafar H Z,Rahmat A,et al.Optimization of microwave-assisted extraction of zerumbone from Zingiber zerumbet L.rhizome and evaluation of antiproliferative activity of optimized extracts[J].Chem Cent J,2017,11:5.
[14]刘瑞林,余佩,陈国宁,等.响应曲面法优化超声-微波协同提取澳洲坚果油的工艺研究[J].西北药学杂志,2017,32(6):718-722.
[15]蒋丽芸,黄玉梅,吴志坚,等.岭南炮天雄的炮制工艺优选[J].中国实验方剂学杂志,2015,21(21):24-27.
[16]张雪,宋玉琴,杨雨婷,等.附子炮制品水煎液对比格犬心脏急性毒性研究[J].中国民族民间医药,2015,24(16):21-23.
[17]宋玉琴,张雪,董艳红,等.附子不同炮制品灌胃Beagle犬的急性毒性研究[J].世界科学技术-中医药现代化,2015,17(7):1432-1437.
[18]覃军,邓广海,陈艳红,等.不同产地炮天雄的质量考察与比较[J].中国药业,2017,26(3):11-14.
[19]Wang M,Yang X B,Zhao J W,et al.Structural characterization and macrophage immunomodulatory activity of a novel polysaccharide from Smilax glabra Roxb[J].Carbohydrate Polym,2017,156:390-402.