气相色谱-串联质谱法测定茶叶中草甘膦残留量的不确定度评定
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摘要
对茶叶中草甘膦残留量的气相色谱-串联质谱法测定过程进行了研究,分析测量过程的不确定度来源,并且对不确定度的各个分量进行评估和合成。此方法主要不确定度来源为标准物质纯度和标准曲线拟合。当茶叶中草甘膦残留量为0.573 mg/kg时,扩展不确定度为0.042 mg/kg,测定结果表示为X=(0.573±0.042)mg/kg,k=2,P=95%。
The determination process of glyphosate residue in tea was studied by the gas chromatography-tandem mass spectrometry(GC-MS),the major source of uncertainty of the determination process was analyzed,and each component of the uncertainty was evaluated and synthesized. The main sources of uncertainty in this method are the purity of standard substance and standard curve fitting. When the content of glyphosate residue was 0.573 mg/kg,the expanded uncertainty was 0.042 mg/kg.The determination result of glyphosate residue content can be expressed as follows:X=(0.573±0.042) mg/kg,k=2,P=95%.
The determination process of glyphosate residue in tea was studied by the gas chromatography-tandem mass spectrometry(GC-MS),the major source of uncertainty of the determination process was analyzed,and each component of the uncertainty was evaluated and synthesized. The main sources of uncertainty in this method are the purity of standard substance and standard curve fitting. When the content of glyphosate residue was 0.573 mg/kg,the expanded uncertainty was 0.042 mg/kg.The determination result of glyphosate residue content can be expressed as follows:X=(0.573±0.042) mg/kg,k=2,P=95%.
引文
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[3]叶美君,朱明,杜颖颖,等.茶园主要除草剂草甘膦和草铵膦概述[J].中国茶叶加工,2017(5/6):32-37.
[4]诸力,陈红平,周苏娟,等.超高效液相色谱-串联质谱法测定不同茶叶中草甘膦、氨甲基膦酸及草铵膦的残留分析化学[J].2015,43(2):271-276.
[5]刘正才,蔡春平,林永辉,等.分散固相萃取/液相色谱-串联质谱法测定茶叶中草甘膦及其代谢物氨甲基膦酸的残留量[J].分析测试学报,2015,34(3):335-340.
[6]陈磊.HPLC-MS/MS同时检测乌龙茶中草铵膦、草甘膦和氨甲基膦酸残留[J].茶叶科学技术,2014(1):21-27.
[7]朱伟.高效液相色谱-串联质谱法测定茶叶中草甘膦残留量的不确定度评价[J].包装与食品机械,2013,31(6):58-60.
[8]Daruich J,Fanny Z,Gimenez M S.Effect of the Herbicide Glyphosate on Enzymatic Activity in Pregnant Rats and Their Fetuses[J].Environmental Reaserch,2001,85(3):226-231.
[9]Fujii T.Transgenerational Effects of Maternal Exposure to Chemicals on the Functional Development of the Brain in the Off-spring[J].Cancer Causes&Control,1997,8(3):524-528.
[10]Dallegrave E,Mantese F D,Coelho R S,et al.The Teratogenic Potential of the Herbicide GlyphosateRoundup in Wistar Rats[J].Toxicology Letters,2003,142(1/2):45-52.
[11]南旭阳,陈浩,张碧双,等.除草剂“草甘膦”对蟾蜍外周血红细胞微核及核异常的影响[J].温州师范学院学报(自然科学版),2000,21(6):57-58.
[12]梁建中,廖培军,黎昌烈.急性草甘膦中毒12例分析[J].现代预防医学,2003,30(6):900.
[13]李波,邓晓军,郭德华,等.高效液相色谱-串联质谱法检测食品中的草甘膦及其主要代谢物氨甲基膦酸残留[J].色谱,2007,25(4):486-490.
[14]中华人民共和国国家质量监督检验检疫总局.测量不确定度评定与表示:JJF 1059.1-2012[S].北京:中国计量出版社,2012.
[15]中国合格评定国家认可委员会.化学分析中不确定度的评估指南:CNAS-GL006[S].北京:中国计量出版社,2018.
[16]中国合格评定国家认可委员会.测量不确定度的要求:CNAS-CL01-G003[S].北京:中国计量出版社,2018.
[17]中华人民共和国国家质量监督检验检疫总局.化学分析测量不确定度评定:JJF 1135-2005[S].北京:中国计量出版社,2005.
[18]中华人民共和国国家质量监督检验检疫总局.植物性产品中草甘膦残留量的测定气相色谱-质谱法:GB/T 23750-2009[S].北京:中国标准出版社,2009.
[19]国家质量监督检验检疫总局.常用玻璃量嚣检定规程:JJG196-2006[S].北京:中国计量出版社,2006.