SDS作用下土壤中四环素类药物的解吸性能
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摘要
高效液相色谱法可同时测定三种四环素类抗生素:四环素(TC)、金霉素(CTC)、差向金霉素(CQC),本文利用该方法分析SDS作用下四环素在土壤中的解吸性能。采用C18色谱柱(3.9 mm×150 mm,5μm),流动相为N,N-二甲基甲酰胺:水:2 mol/L磷酸溶液=28:70:2,紫外测定波长λ=365 nm,柱温35℃。四环素、金霉素、差向金霉素保留时间分别为1.40 min、10.64 min、8.62 min。实验结果表明,加入表面活性剂SDS对土壤中单一的四环素和三种四环素类药物都有解吸作用;在表面活性剂浓度为6 cmc,作用时间为10 h的条件下,三种四环素类抗生素解吸作用都达到了60%以上。
High Performance Liquid Chromatography(HPLC) was available to detect tetracycline(TC), chlorotetracycline(CTC) and chlorquatrimycline(CQC) simultaneously. In this paper, HPLC was used to analyze the desorption of tetracycline in soil influenced by sodium dodecyl sulfat(SDS). The analysis was performed on a C18 column(3.9 mm×150 mm,5 μm)with a mobile phase N,N-dimethylformamide: water: phosphoric acid solution(2 mol/L)=28:70:2. The UV detection wavelength(λ) was set as 365 nm and the column temperature was set at 35 ℃. The retention time of TC, CTC and CQC was 1.40 min, 10.64 min and 8.62 min,respectively.It was found that the surfactantSDS accelerated the desorption processof single or mixed tetracycline. If SDS concentration was 6 times of critical micelle concentrations(6 cmc),the desorption efficiency of three tetracycline antibioticsreached more than 60 % after 10 hours.
High Performance Liquid Chromatography(HPLC) was available to detect tetracycline(TC), chlorotetracycline(CTC) and chlorquatrimycline(CQC) simultaneously. In this paper, HPLC was used to analyze the desorption of tetracycline in soil influenced by sodium dodecyl sulfat(SDS). The analysis was performed on a C18 column(3.9 mm×150 mm,5 μm)with a mobile phase N,N-dimethylformamide: water: phosphoric acid solution(2 mol/L)=28:70:2. The UV detection wavelength(λ) was set as 365 nm and the column temperature was set at 35 ℃. The retention time of TC, CTC and CQC was 1.40 min, 10.64 min and 8.62 min,respectively.It was found that the surfactantSDS accelerated the desorption processof single or mixed tetracycline. If SDS concentration was 6 times of critical micelle concentrations(6 cmc),the desorption efficiency of three tetracycline antibioticsreached more than 60 % after 10 hours.
引文
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[8]Sokolic M, Filipovic B, Pokorny. M. High-performance liquidchromatographic procedures in monitoring the production and qualitycontrol of chlortetracycline[J]. J Chromatogr A, 1990, 509(1):189-193.
[9]周武杰,刘金彦,胡亚运.表面活性剂对土壤中残留头孢他啶的解吸作用[J].中国抗生素杂志,2013,38(12):931-935.
[10]Bettahar A, Schafer G, Baviere M. An optimized surfactantformu-lation for the remediation of diesel oil polluted sandyaquifers[J]. En-viron Sci Technol, 1999, 33:1269-1273.
[11]牛迪,岑桂秋,李嘉诚,等.不同结构头基对阴/非离子表面活性剂混合体系胶团化行为的影响[J].精细化工, 2013,30(10):1102-1107.
[12]郭喜丰,肖广全,马丽莉,等.超声波降解四环素类抗生素废水[J],环境工程学报,2014,8(4):1503-1509.
[13]张婷.四环素类抗生素在土壤中的吸附/解吸行为研究[D].辽宁工程技术大学,硕士学位论文.2014.
[2]Sarmah AK, Meyer MT, Boxalla BA. A global perspective on theuse, sales, exposure pathways, occurrence, fate and effects of veteri-nary antibiotics(VAs)in the envi-ronment[J]. Chemosphere, 2006,65(5):725-759.
[3]刘雨霞,鲍艳宇.土壤中四环素类抗生素污染研究进展[J].环境污染与防治,2011,33(8):81-86,91.
[4]Glen RB, Helge R, Deborah AG, et al. Pharmaceuticals and per-sonal care products(PPCPs)in surface and treated waters ofLouisiana, USA and Ontario, Canada[J].Sci Total Environ,2003,311:135-149.
[5]Edwanrds DA, Luthy RG, Liu Z. Solubilization of polycyclic aro-matic hydrocarbons in micellar nonionic surfactant solutions[J].Envi-ron Sci Technol,1991,25:127-133.
[6]Park JL, Abriola LK, Weber WJ, et al. Solubilization rates nickelin micellar solutions of nonionic surfactants[J].Enviro Sci Technol,2000,34:476-482.
[7]姚玉玲,李绍波,万喻平,等.高效液相色谱同时测定饲料中三种抗生素[J].化学研究与应用.2009,21(1):26-27.
[8]Sokolic M, Filipovic B, Pokorny. M. High-performance liquidchromatographic procedures in monitoring the production and qualitycontrol of chlortetracycline[J]. J Chromatogr A, 1990, 509(1):189-193.
[9]周武杰,刘金彦,胡亚运.表面活性剂对土壤中残留头孢他啶的解吸作用[J].中国抗生素杂志,2013,38(12):931-935.
[10]Bettahar A, Schafer G, Baviere M. An optimized surfactantformu-lation for the remediation of diesel oil polluted sandyaquifers[J]. En-viron Sci Technol, 1999, 33:1269-1273.
[11]牛迪,岑桂秋,李嘉诚,等.不同结构头基对阴/非离子表面活性剂混合体系胶团化行为的影响[J].精细化工, 2013,30(10):1102-1107.
[12]郭喜丰,肖广全,马丽莉,等.超声波降解四环素类抗生素废水[J],环境工程学报,2014,8(4):1503-1509.
[13]张婷.四环素类抗生素在土壤中的吸附/解吸行为研究[D].辽宁工程技术大学,硕士学位论文.2014.