草莓育种新动态及发展趋势
|
摘要
草莓作为重要的浆果类作物之一,其栽培面积和产量一直居于世界小浆果生产的首位,业已成为我国效益最好的农业产业。栽培草莓选育始于19世纪初的英国,我国草莓育种起步较晚始于20世纪50年代,本文按照时间节点介绍了国内外草莓育种历程及代表性草莓品种。八倍体栽培草莓基因组中亚基因组间存在高度同源性,到目前为止未组装出较为完整的参考基因组;二倍体森林草莓基因组经过多次修订具有较高的完整性和精密性。随着测序技术的发展,大量的草莓品质和抗性相关的分子标记被开发,这些标记也被应用于草莓育种项目中,大大提高了育种效率。我国草莓育种存在亲本遗传背景狭窄、育种方法单一等问题,针对这些问题提出了我国草莓育种未来方向和发展趋势。
Strawberry is one of the most important berry crops worldwide,and is always ranking first in cultivation area and yield harvest among the small berries.Nowadays the strawberry planting has become one of profiling agricultural industries in China.The breeding of cultivated strawberries began in England in the early 19 th century,while it began in the 1950 s in China.According to the timeline,the breeding history and representative varieties of strawberry at home and abroad were described in this paper.So far a relatively complete reference genome has not been assembled,due to the high degree of homology between subgenomes in the octoploid strawberry genome.The diploid strawberry(Fragaria vesca)genome has high integrity and precision after revision.With the development of sequencing technology,a large number of molecular markers related to strawberry quality and resistance have been developed.These markers have also been used in strawberry breeding program and greatly improved the breeding efficiency.There are some problems in strawberry breeding in China,such as narrow parental genetic background and single breeding method.In view of these problems,the future direction and development trend of strawberry breeding in China were reviewed.
Strawberry is one of the most important berry crops worldwide,and is always ranking first in cultivation area and yield harvest among the small berries.Nowadays the strawberry planting has become one of profiling agricultural industries in China.The breeding of cultivated strawberries began in England in the early 19 th century,while it began in the 1950 s in China.According to the timeline,the breeding history and representative varieties of strawberry at home and abroad were described in this paper.So far a relatively complete reference genome has not been assembled,due to the high degree of homology between subgenomes in the octoploid strawberry genome.The diploid strawberry(Fragaria vesca)genome has high integrity and precision after revision.With the development of sequencing technology,a large number of molecular markers related to strawberry quality and resistance have been developed.These markers have also been used in strawberry breeding program and greatly improved the breeding efficiency.There are some problems in strawberry breeding in China,such as narrow parental genetic background and single breeding method.In view of these problems,the future direction and development trend of strawberry breeding in China were reviewed.
引文
[1]王桂霞,张运涛,董静,张利喜.中国草莓育种的回顾和展望.植物遗传资源学报,2008,9(2):272-276Wang G X,Zhang Y T,Dong J,Zhang L X.Retrospection and prospect of strawberry breeding in China.Journal of Plant Genetic Resources,2008,9(2):272-276
[2]齐永志,贾薇,苏媛,刘雪静,王宁,马燕会,甄文超.草莓新品种‘连达’.园艺学报,2017,44(7):1419-1420Qi Y Z,Jia W,Su Y,Liu X J,Wang N,Ma Y H,Zhen W C.Anew strawberry cultivar‘Lianda’.Acta Horticulturae Sinica,2017,44(7):1419-1420
[3]常琳琳,董静,钟传飞,孙健,孙瑞,石琨,王桂霞,张运涛.中国育成草莓品种的系谱分析.果树学报,2018,35(2):158-167Chang L L,Dong J,Zhong C F,Sun J,Sun R,Shi K,Wang G X,Zhang Y T.Pedigree analysis of strawberry cultivars released in China.Journal of Fruit Science,2018,35(2):158-167
[4]Darrow G.The Strawberry.History,Breeding and Physiology.New York:Holt,Rinehart and Winston,1966
[5]Rubinstein J.Fragaria×ananassa:past,present and future production of the modern strawberry.(2015-11-17)[2018-10-15].http://hdl.handle.net/11299/175838
[6]Schreier P.Quantitative composition of volatile constituents in cultivated strawberries,Fragaria ananassa cv.Senga Sengana,Senga Litessa and Senga Gourmella.Journal of the Science of Food and Agriculture,1980,31(5):487-494
[7]Whitehouse A B,Simpson D W,Johnson A W,Mcleary KJ,Passey A J,Troop S W.‘Malling Centenary’,a short-day strawberry cultivar from NIAB-EMR.Acta Horticulturae,2017,1156:185-188
[8]Hancock J F,Edger P P,Callow P W,Thomas H,Finn CE.Generating a unique germplasm base for the breeding of dayneutral strawberry cultivars.HortScience,2018,53(7):1069-1071
[9]Simpson D.The economic importance of strawberry crops.the genomes of rosaceous berries and their wild relatives.Springer Cham,2018:1-7
[10]Whitaker V M,Lee S,Osorio L F,Verma S,Roach J A,Mangandi J.Advances in strawberry breeding at the University of Florida.Acta Horticulturae,2017,1156:1-6
[11]Nagano S,Shirasawa K,Maeda F,Watanabe M.Challenge to genomic selection in strawberry at four breeding stations in Japan.Acta Horticulturae,2017,1203:1-8
[12]赵密珍.日本四季草莓育种与夏秋草莓生产概况.中国果树,2006(2):61-62Zhao M Z.General situation of strawberry breeding and summer and autumn production in Japan.China Fruits,2006(2):61-62
[13]Takuya W,Koichiro O,Soichiro N,Sachiko I,Hideyuki S,Miyuki M.Development and characterization of a strawberry MAGIC population derived from crosses with six strawberry cultivars.Breeding Science,2017,67(4):370-381
[14]Bentvelsen G C M,Bouw E,Zanten J E V V.Breeding strawberries(Fragaria×ananassa duch.)from seed.Acta Horticulturae,1997,439:149-154
[15]Maruo T,Ito Y,Ishikawa M,Kamisoyama D,Ohmameuda M,Ogura Y.About the practical use of the seed propagation type strawberry(F.×ananassa duch.)cultivation system(using june-bearing cultivar).Hort Research,2007,61(1):1-10
[16]Morishita M.status of strawberry breeding and cultivation in Japan.Journal of the Medical Library Association,2014,94(2):145-51
[17]Mori T,Kohori J,Kitamura H,Inokuchi T,Kato I,Sone K.Development of F1-hybrid strawberry of seed propagation type named‘yotsuboshi’by collaborative breeding among institutes.Hort Research,2015,14(4):409-418
[18]Kang C,Darwish O,Geretz A,Shahan R,Alkharouf N,Liu Z.Genome-scale transcriptomic insights into early-stage fruit development in woodland strawberry Fragaria vesca.Plant Cell,2013,25(6):1960-1978
[19]Shulaev V,Sargent D J,Crowhurst R N,Mockler T C,Folkerts O,Delcher A L,Jaiswal P,Mockaitis K,Liston A,Mane S P,Burns P,Davis T M,Slovin J P,Bassil N,Hellens R P,Evans C,Harkins T,Kodira C,Desany B,Crasta O R,Jensen R V,Allan A C,Michael T P,Setubal J C,Celton J M,Rees D J G,Williams K P,Holt S H,Rojas J J R,Chatterjee M,Liu B,Silva H,Meisel L,Adato A,Filichkin S A,Troggio M,Viola R,Ashman T L,Wang H,Dharmawardhana P,Elser J,Raja R,Priest H D,Bryant D W,Fox S E,Givan S A,Wilhelm L J,Naithani S,Christoffels A,Salama D Y,Carter J,Girona E L,Zdepski A,Wang W Q,Kerstetter R A,Schwab W,Korban SS,Davik J,Monfort A,Denoyes-Rothan B,Arus P,Mittler R,Flinn B,Aharoni A,Bennetzen J L,Salzberg S L,Dickerman A W,Velasco R,Borodovsky M,Veilleux R E,Folta K M.The genome of woodland strawberry(Fragaria vesca).Nature Genetics,2010,43:109-116
[20]Darwish O,Shahan R,Liu Z,Slovin J P,Alkharouf N W.Reannotation of the woodland strawberry(Fragaria vesca)genome.BMC Genomics,2015,16:29-38
[21]Tennessen J A,Govindarajulu R,Ashman T L,Liston A.Evolutionary origins and dynamics of octoploid strawberry subgenomes revealed by dense targeted capture linkage maps.Genome Biology and Evolution,2014,6(12):3295-313
[22]Edger P P,VanBuren R,Colle M,Poorten T J,Wai C M,Niederhuth C E,Alger E I,Ou S,Acharya C B,Wang J,Callow P,McKain M R,Shi J,Collier C,Xiong Z,Mower J P,Slovin J P,Hyt?nen T,Jiang N,Childs K L,Knapp S J.Singlemolecule sequencing and optical mapping yields an improved genome of woodland strawberry(Fragaria vesca)with chromosome-scale contiguity.GigaScience,2018,7(2):1-7
[23]Davis T M,Denoyes-Rothan B,Lerceteau-K?hler E.Strawberry//Kole C.Fruits and Nuts.Genome Mapping and Molecular Breeding in Plants.Berlin,Heidelberg:Springer,2007:189-207
[24]Hirakawa H,Shirasawa K,Kosugi S,Tashiro K,Nakayama S,Yamada M,Kohara M,Watanabe A,Kishida Y,Fujishiro T,Tsuruoka H,Minami C,Sasamoto S,Kato M,Nanri K,Komaki A,Yanagi T,Guoxin Q,Maeda F,Ishikawa M,Kuhara S,Sato S,Tabata S,Isobe S N.Dissection of the octoploid strawberry genome by deep sequencing of the genomes of Fragaria species.DNA Research,2014,21(2):169-181
[25]Davis T M,Yu HA.linkage map of the diploid strawberry,Fragaria vesca.Journal of Heredity,1997,88(3):215-221
[26]Sargent D J,Davis T M,Tobutt K R,Wilkinson M J,Battey N H,Simpson D W.A genetic linkage map of microsatellite,gene-specific and morphological markers in diploid Fragaria.Theoretical and Applied Genetics,2004,109(7):1385-1391
[27]Sargent D J,Yang Y,?urbanovski N,Bianco L,Buti M,Velasco R,Giongo L,Davis T M.HaploSNP affinities and linkage map positions illuminate subgenome composition in the octoploid,cultivated strawberry(Fragaria×ananassa).Plant Science,2016,242:140-150
[28]Lerceteau-K?hler E,Guerin G,Laigret F,Denoyes-Rothan B.Characterization of mixed disomic and polysomic inheritance in the octoploid strawberry(Fragaria×ananassa)using AFLPmapping.Theoretical and Applied Genetics,2003,107(4):619-628
[29]Sargent D J,Fernandéz-Fernandéz F,Ruiz-Roja J J,Sutherland B G,Passey A,Whitehouse A B.A genetic linkage map of the cultivated strawberry(Fragaria×ananassa)and its comparison to the diploid Fragaria reference map.Molecular Breeding,2009,24(3):293-303
[30]Sargent D,Kuchta P,Girona E,Zhang H,Davis T,Celton JM,Marchese A,Korbin M,Folta K M,Shulaev V,Simpson W.Simple sequence repeat marker development and mapping targeted to previously unmapped regions of the strawberry genome sequence.Plant Genome,2011,4(3):165-177
[31]van Dijk T,Pagliarani G,Pikunova A,Noordijk Y,YilmazTemel H,Meulenbroek B,Visser R G,van de Weg E.Genomic rearrangements and signatures of breeding in the allo-octoploid strawberry as revealed through an allele dose based SSR linkage map.BMC Plant Biology,2014,14(1):55
[32]Bassil N V,Davis T M,Zhang H,Ficklin S,Mittmann M,Webster T,Mahoney L,Wood D,Alperin E S,Rosyara U R,Koehorst-vanc Putten H,Monfort A,Sargent D J,Amaya I,Denoyes B,Bianco L,van Dijk T,Pirani A,Iezzoni A,Main D,Peace C,Yang Y,Whitaker V,Verma S,Bellon L,Brew F,Herrera R,van de Weg E.Development and preliminary evaluation of a 90K Axiom?SNP array for the allo-octoploid cultivated strawberry Fragaria×ananassa.BMC Genomics,2015,16(1):155
[33]Verma S,Bassil N,van de Weg E,Harrison R J,Monfort A,Hidalgo J M,Amaya I,Denoyes B,Mahoney L L,Davis T M,Fan Z,Knapp S,Whitaker V M.Development and evaluation of the Axiom?IStraw35 384HT array for the allo-octoploid cultivated strawberry Fragaria ananassa.Acta Horticulturae,2017,1156:75-82
[34]Ma honey L L,Sargent D J,Abebe-Akele F,Wood D J,Ward J A,Bassil N V,Hancock J F,Folta K M,Davis T M.Ahigh-density linkage map of the ancestral diploid strawberry,Fragaria iinumae,constructed with single nucleotide polymorphism markers from the IStraw 90 array and genotyping by sequencing.Plant Genome,2016,9(2):1-14
[35]Roach J A,Verma S,Peres N A,Jamieson A R,van De Weg WE,Bink M C A M,Bassil N V,Lee S,Whitaker V M.FaRXf1:a locus conferring resistance to angular leaf spot caused by Xanthomonas fragariae in octoploid strawberry.Theoretical and Applied Genetics,2016,129(6):1191-1201
[36]Mangandi J,Verma S,Osorio L,Peres N A,Van de Weg E,Whitaker V M.Pedigree-based analysis in a multiparental population of octoploid strawberry reveals QTL alleles conferring resistance to Phytophthora cactorum..G3 Genesgenetics,2017,7(6):1707-1719
[37]Anciro A,Mangandi J,Verma S,Whitaker V,Lee S.Identification of quantitative trait loci and molecular markers for resistance to Colletotrichum crown rot in strawberry.Phytopathology,2016,106(S2):6
[38]Lerceteau-K?hler E,Moing A,Guérin G,Renaud C,Petit A,Rothan C,Denoyes B.Genetic dissection of fruit quality traits in the octoploid cultivated strawberry highlights the role of homoeo-QTL in their control.Theoretical and Applied Genetics,2012,124(6):1059-1077
[39]Castro P,Lewers K S.Identification of quantitative trait loci(QTL)for fruit-quality traits and number of weeks of flowering in the cultivated strawberry.Molecular Breeding,2016,36(10):138
[40]van Dijk T,Pagliarani G,Pikunova A,Noordijk Y,YilmazTemel H,Meulenbroek B,Visser R G,Van de Weg E.Genomic rearrangements and signatures of breeding in the allo-octoploid strawberry as revealed through an allele dose based SSR linkage map.BMC Plant Biology,2014,14(1):55
[41]Verma S,Osorio L F,Lee S,Bassil N V,Whitaker VM.Genome-assisted breeding in the octoploid strawberry//Hyt?nen T,Graham J,Harrison R.The Genomes of Rosaceous Berries and Their Wild Relatives.https://doi.org/10.1007/978-3-319-76020-9_12
[42]Chambers A H,Pillet J,Plotto A,Bai J,Whitaker V M,Folta K M.Identification of a strawberry flavor gene candidate using an integrated genetic-genomicanalytical chemistry approach.BMC Genomics,2014,15(1):217
[43]Sánchez-Sevilla J F,Cruz-Rus E,Valpuesta V,Botella MA,Amaya I.Deciphering gamma-decalactone biosynthesis in strawberry fruit using a combination of genetic mapping,RNA-Seq and eQTL analyses.BMC Genomics,2014,15(1):218
[44]Zorrilla-Fontanesi Y,Cabeza A,Domínguez P,Medina JJ,Valpuesta V,Denoyes-Rothan B,Sánchez-Sevilla J F,Amaya I.Quantitative trait loci and underlying candidate genes controlling agronomical and fruit quality traits in octoploid strawberry(Fragaria×ananassa).Theoretical and Applied Genetics,2012,123(5):755-778
[45]Gaston A,Perrotte J,Lerceteau-K?hler E,Rousseau-Gueutin M,Petit A,Hernould M,Rothan C,Denoyes B.PFRU,a single dominant locus regulates the balance between sexual and asexual plant reproduction in cultivated strawberry.Journal of Experimental Botany,2013,64(7):1837-1848
[46]Perrotte J,Gaston A,Potier A,Petit A,Rothan C,Denoyes B.Narrowing down the single homoeologous FaPFRU locus controlling flowering in octoploid strawberry using a selective mapping strategy.Plant Biotechnology Journal,2016,14(11):2176-2189
[47]Verma S,Roach J,Mangandi J,Lee S,Salinas N,Bassil N,Bink M,van de Weg E,Peace C,Iezzoni A,Whitaker V.DNA-informed strawberry breeding in RosBREED//San Diego,CA,USA:Plant and animal genome XXIV conference.[2016-08-13]https://pag.confex.com/pag/xxiv/webprogram/Paper18784.html
[48]Sargent D J,Hadonou A M,Simpson D W.Development and characterization of polymorphic microsatellite markers from Fragaria viridis,a wild diploid strawberry.Molecular Ecology Resources,2003,3(4):550-552
[49]James C M,Wilson F,Hadonou A M,Tobutt K R.Isolation and characterization of polymorphic microsatellites in diploid strawberry(Fragaria vesca L.)for mapping,diversity studies and clone identification.Molecular Ecology Notes,2003,3(2):171-173
[50]Davis T M,DiMeglio L M,Yang R,Styan S M N,Lewers K S.Assessment of SSR marker transfer from the cultivated strawberry to diploid strawberry species:functionality,linkage group assignment,and use in diversity analysis.The Journal of the American Society for Horticultural Science,2006,131(4):506-512
[51]Sargent D J,Passey T,Surbanovski N,Lopez Girona E,Kuchta P,Davik J,Harrison R,Passey A,Whitehouse A B,Simpson D W.A microsatellite linkage map for the cultivated strawberry(Fragaria×ananassa)suggests extensive regions of homozygosity in the genome that may have resulted from breeding and selection.Theoretical and Applied Genetics,2012,124(7):1229-1240
[52]Isobe S N,Hirakawa H,Sato S,Maeda F,Ishikawa M,Mori T,Yamamoto Y,Shirasawa K,Kimura M,Fukami M,Hashizume F,Tsuji T,Sasamoto S,Kato M,Nanri K,Tsuruoka H,Minami C,Takahashi C,Wada T,Ono A,Kawashima K,Nakazaki N,Kishida Y,Kohara M,Nakayama S,Yamada M,Fujishiro T,Watanabe A,Tabata S.Construction of an integrated high density simple sequence repeat linkage map in cultivated strawberry(Fragaria×ananassa)and its applicability.DNAResearch,2013,20(1):79-92
[53]Sánchez-Sevilla J F,Horvath A,Botella M A,Gaston A,Folta K,Kilian A,Denoyes B,Amaya I.Diversity arrays technology(DArT)marker platforms for diversity analysis and linkage mapping in a complex crop,the octoploid cultivated strawberry(Fragaria×ananassa).PLoS One,2015,10(12):e0144960
[54]Noh Y H,Lee S,Whitaker V M,Cearley K R,Cha J S.Ahigh-throughput marker-assisted selection system combining rapid DNA extraction and high-resolution melting and simple sequence repeat analysis:strawberry as a model for fruit crops.Journal of Berry Research,2017,7(1):23-31
[55]Lerceteau-K?hler E,Guérin G,Denoyes-Rothan B.Identification of SCAR markers linked to Rca2 anthracnose resistance gene and their assessment in strawberry germplasm.Theoretical and Applied Genetics,2005,111(5):862-870
[56]Mathey M M.Phenotyping diverse strawberry(Fragaria spp.)germplasm for aid in marker-assisted breeding,and marker-trait association for red stele(Phytophthora fragariae)resistance marker Rpf1.Oregon:Oregon State University,USA,2013
[57]Salinas N.Validation of molecular markers associated with perpetual flowering(PF)and soluble solids content(SSC)in strawberry(Fragaria×ananassa Duch.ex Rozier).Oregon:Oregon State University,USA,2015
[58]Meuwissen T H E,Hayes B J,Goddard M E.Prediction of total genetic value using genome-wide dense marker maps.Genetics,2001,157(4):1819-1829
[59]Gezan S,Osorio L F,Verma S,Whitaker V M.An experimental validation of genomic selection in octoploid strawberry.Horticulture Research,2017,4:16070
[60]Asker S.Some viewpoints on Fragaria×Potentilla intergeneric hybridization.Hereditas,1971,67(2):181-190
[2]齐永志,贾薇,苏媛,刘雪静,王宁,马燕会,甄文超.草莓新品种‘连达’.园艺学报,2017,44(7):1419-1420Qi Y Z,Jia W,Su Y,Liu X J,Wang N,Ma Y H,Zhen W C.Anew strawberry cultivar‘Lianda’.Acta Horticulturae Sinica,2017,44(7):1419-1420
[3]常琳琳,董静,钟传飞,孙健,孙瑞,石琨,王桂霞,张运涛.中国育成草莓品种的系谱分析.果树学报,2018,35(2):158-167Chang L L,Dong J,Zhong C F,Sun J,Sun R,Shi K,Wang G X,Zhang Y T.Pedigree analysis of strawberry cultivars released in China.Journal of Fruit Science,2018,35(2):158-167
[4]Darrow G.The Strawberry.History,Breeding and Physiology.New York:Holt,Rinehart and Winston,1966
[5]Rubinstein J.Fragaria×ananassa:past,present and future production of the modern strawberry.(2015-11-17)[2018-10-15].http://hdl.handle.net/11299/175838
[6]Schreier P.Quantitative composition of volatile constituents in cultivated strawberries,Fragaria ananassa cv.Senga Sengana,Senga Litessa and Senga Gourmella.Journal of the Science of Food and Agriculture,1980,31(5):487-494
[7]Whitehouse A B,Simpson D W,Johnson A W,Mcleary KJ,Passey A J,Troop S W.‘Malling Centenary’,a short-day strawberry cultivar from NIAB-EMR.Acta Horticulturae,2017,1156:185-188
[8]Hancock J F,Edger P P,Callow P W,Thomas H,Finn CE.Generating a unique germplasm base for the breeding of dayneutral strawberry cultivars.HortScience,2018,53(7):1069-1071
[9]Simpson D.The economic importance of strawberry crops.the genomes of rosaceous berries and their wild relatives.Springer Cham,2018:1-7
[10]Whitaker V M,Lee S,Osorio L F,Verma S,Roach J A,Mangandi J.Advances in strawberry breeding at the University of Florida.Acta Horticulturae,2017,1156:1-6
[11]Nagano S,Shirasawa K,Maeda F,Watanabe M.Challenge to genomic selection in strawberry at four breeding stations in Japan.Acta Horticulturae,2017,1203:1-8
[12]赵密珍.日本四季草莓育种与夏秋草莓生产概况.中国果树,2006(2):61-62Zhao M Z.General situation of strawberry breeding and summer and autumn production in Japan.China Fruits,2006(2):61-62
[13]Takuya W,Koichiro O,Soichiro N,Sachiko I,Hideyuki S,Miyuki M.Development and characterization of a strawberry MAGIC population derived from crosses with six strawberry cultivars.Breeding Science,2017,67(4):370-381
[14]Bentvelsen G C M,Bouw E,Zanten J E V V.Breeding strawberries(Fragaria×ananassa duch.)from seed.Acta Horticulturae,1997,439:149-154
[15]Maruo T,Ito Y,Ishikawa M,Kamisoyama D,Ohmameuda M,Ogura Y.About the practical use of the seed propagation type strawberry(F.×ananassa duch.)cultivation system(using june-bearing cultivar).Hort Research,2007,61(1):1-10
[16]Morishita M.status of strawberry breeding and cultivation in Japan.Journal of the Medical Library Association,2014,94(2):145-51
[17]Mori T,Kohori J,Kitamura H,Inokuchi T,Kato I,Sone K.Development of F1-hybrid strawberry of seed propagation type named‘yotsuboshi’by collaborative breeding among institutes.Hort Research,2015,14(4):409-418
[18]Kang C,Darwish O,Geretz A,Shahan R,Alkharouf N,Liu Z.Genome-scale transcriptomic insights into early-stage fruit development in woodland strawberry Fragaria vesca.Plant Cell,2013,25(6):1960-1978
[19]Shulaev V,Sargent D J,Crowhurst R N,Mockler T C,Folkerts O,Delcher A L,Jaiswal P,Mockaitis K,Liston A,Mane S P,Burns P,Davis T M,Slovin J P,Bassil N,Hellens R P,Evans C,Harkins T,Kodira C,Desany B,Crasta O R,Jensen R V,Allan A C,Michael T P,Setubal J C,Celton J M,Rees D J G,Williams K P,Holt S H,Rojas J J R,Chatterjee M,Liu B,Silva H,Meisel L,Adato A,Filichkin S A,Troggio M,Viola R,Ashman T L,Wang H,Dharmawardhana P,Elser J,Raja R,Priest H D,Bryant D W,Fox S E,Givan S A,Wilhelm L J,Naithani S,Christoffels A,Salama D Y,Carter J,Girona E L,Zdepski A,Wang W Q,Kerstetter R A,Schwab W,Korban SS,Davik J,Monfort A,Denoyes-Rothan B,Arus P,Mittler R,Flinn B,Aharoni A,Bennetzen J L,Salzberg S L,Dickerman A W,Velasco R,Borodovsky M,Veilleux R E,Folta K M.The genome of woodland strawberry(Fragaria vesca).Nature Genetics,2010,43:109-116
[20]Darwish O,Shahan R,Liu Z,Slovin J P,Alkharouf N W.Reannotation of the woodland strawberry(Fragaria vesca)genome.BMC Genomics,2015,16:29-38
[21]Tennessen J A,Govindarajulu R,Ashman T L,Liston A.Evolutionary origins and dynamics of octoploid strawberry subgenomes revealed by dense targeted capture linkage maps.Genome Biology and Evolution,2014,6(12):3295-313
[22]Edger P P,VanBuren R,Colle M,Poorten T J,Wai C M,Niederhuth C E,Alger E I,Ou S,Acharya C B,Wang J,Callow P,McKain M R,Shi J,Collier C,Xiong Z,Mower J P,Slovin J P,Hyt?nen T,Jiang N,Childs K L,Knapp S J.Singlemolecule sequencing and optical mapping yields an improved genome of woodland strawberry(Fragaria vesca)with chromosome-scale contiguity.GigaScience,2018,7(2):1-7
[23]Davis T M,Denoyes-Rothan B,Lerceteau-K?hler E.Strawberry//Kole C.Fruits and Nuts.Genome Mapping and Molecular Breeding in Plants.Berlin,Heidelberg:Springer,2007:189-207
[24]Hirakawa H,Shirasawa K,Kosugi S,Tashiro K,Nakayama S,Yamada M,Kohara M,Watanabe A,Kishida Y,Fujishiro T,Tsuruoka H,Minami C,Sasamoto S,Kato M,Nanri K,Komaki A,Yanagi T,Guoxin Q,Maeda F,Ishikawa M,Kuhara S,Sato S,Tabata S,Isobe S N.Dissection of the octoploid strawberry genome by deep sequencing of the genomes of Fragaria species.DNA Research,2014,21(2):169-181
[25]Davis T M,Yu HA.linkage map of the diploid strawberry,Fragaria vesca.Journal of Heredity,1997,88(3):215-221
[26]Sargent D J,Davis T M,Tobutt K R,Wilkinson M J,Battey N H,Simpson D W.A genetic linkage map of microsatellite,gene-specific and morphological markers in diploid Fragaria.Theoretical and Applied Genetics,2004,109(7):1385-1391
[27]Sargent D J,Yang Y,?urbanovski N,Bianco L,Buti M,Velasco R,Giongo L,Davis T M.HaploSNP affinities and linkage map positions illuminate subgenome composition in the octoploid,cultivated strawberry(Fragaria×ananassa).Plant Science,2016,242:140-150
[28]Lerceteau-K?hler E,Guerin G,Laigret F,Denoyes-Rothan B.Characterization of mixed disomic and polysomic inheritance in the octoploid strawberry(Fragaria×ananassa)using AFLPmapping.Theoretical and Applied Genetics,2003,107(4):619-628
[29]Sargent D J,Fernandéz-Fernandéz F,Ruiz-Roja J J,Sutherland B G,Passey A,Whitehouse A B.A genetic linkage map of the cultivated strawberry(Fragaria×ananassa)and its comparison to the diploid Fragaria reference map.Molecular Breeding,2009,24(3):293-303
[30]Sargent D,Kuchta P,Girona E,Zhang H,Davis T,Celton JM,Marchese A,Korbin M,Folta K M,Shulaev V,Simpson W.Simple sequence repeat marker development and mapping targeted to previously unmapped regions of the strawberry genome sequence.Plant Genome,2011,4(3):165-177
[31]van Dijk T,Pagliarani G,Pikunova A,Noordijk Y,YilmazTemel H,Meulenbroek B,Visser R G,van de Weg E.Genomic rearrangements and signatures of breeding in the allo-octoploid strawberry as revealed through an allele dose based SSR linkage map.BMC Plant Biology,2014,14(1):55
[32]Bassil N V,Davis T M,Zhang H,Ficklin S,Mittmann M,Webster T,Mahoney L,Wood D,Alperin E S,Rosyara U R,Koehorst-vanc Putten H,Monfort A,Sargent D J,Amaya I,Denoyes B,Bianco L,van Dijk T,Pirani A,Iezzoni A,Main D,Peace C,Yang Y,Whitaker V,Verma S,Bellon L,Brew F,Herrera R,van de Weg E.Development and preliminary evaluation of a 90K Axiom?SNP array for the allo-octoploid cultivated strawberry Fragaria×ananassa.BMC Genomics,2015,16(1):155
[33]Verma S,Bassil N,van de Weg E,Harrison R J,Monfort A,Hidalgo J M,Amaya I,Denoyes B,Mahoney L L,Davis T M,Fan Z,Knapp S,Whitaker V M.Development and evaluation of the Axiom?IStraw35 384HT array for the allo-octoploid cultivated strawberry Fragaria ananassa.Acta Horticulturae,2017,1156:75-82
[34]Ma honey L L,Sargent D J,Abebe-Akele F,Wood D J,Ward J A,Bassil N V,Hancock J F,Folta K M,Davis T M.Ahigh-density linkage map of the ancestral diploid strawberry,Fragaria iinumae,constructed with single nucleotide polymorphism markers from the IStraw 90 array and genotyping by sequencing.Plant Genome,2016,9(2):1-14
[35]Roach J A,Verma S,Peres N A,Jamieson A R,van De Weg WE,Bink M C A M,Bassil N V,Lee S,Whitaker V M.FaRXf1:a locus conferring resistance to angular leaf spot caused by Xanthomonas fragariae in octoploid strawberry.Theoretical and Applied Genetics,2016,129(6):1191-1201
[36]Mangandi J,Verma S,Osorio L,Peres N A,Van de Weg E,Whitaker V M.Pedigree-based analysis in a multiparental population of octoploid strawberry reveals QTL alleles conferring resistance to Phytophthora cactorum..G3 Genesgenetics,2017,7(6):1707-1719
[37]Anciro A,Mangandi J,Verma S,Whitaker V,Lee S.Identification of quantitative trait loci and molecular markers for resistance to Colletotrichum crown rot in strawberry.Phytopathology,2016,106(S2):6
[38]Lerceteau-K?hler E,Moing A,Guérin G,Renaud C,Petit A,Rothan C,Denoyes B.Genetic dissection of fruit quality traits in the octoploid cultivated strawberry highlights the role of homoeo-QTL in their control.Theoretical and Applied Genetics,2012,124(6):1059-1077
[39]Castro P,Lewers K S.Identification of quantitative trait loci(QTL)for fruit-quality traits and number of weeks of flowering in the cultivated strawberry.Molecular Breeding,2016,36(10):138
[40]van Dijk T,Pagliarani G,Pikunova A,Noordijk Y,YilmazTemel H,Meulenbroek B,Visser R G,Van de Weg E.Genomic rearrangements and signatures of breeding in the allo-octoploid strawberry as revealed through an allele dose based SSR linkage map.BMC Plant Biology,2014,14(1):55
[41]Verma S,Osorio L F,Lee S,Bassil N V,Whitaker VM.Genome-assisted breeding in the octoploid strawberry//Hyt?nen T,Graham J,Harrison R.The Genomes of Rosaceous Berries and Their Wild Relatives.https://doi.org/10.1007/978-3-319-76020-9_12
[42]Chambers A H,Pillet J,Plotto A,Bai J,Whitaker V M,Folta K M.Identification of a strawberry flavor gene candidate using an integrated genetic-genomicanalytical chemistry approach.BMC Genomics,2014,15(1):217
[43]Sánchez-Sevilla J F,Cruz-Rus E,Valpuesta V,Botella MA,Amaya I.Deciphering gamma-decalactone biosynthesis in strawberry fruit using a combination of genetic mapping,RNA-Seq and eQTL analyses.BMC Genomics,2014,15(1):218
[44]Zorrilla-Fontanesi Y,Cabeza A,Domínguez P,Medina JJ,Valpuesta V,Denoyes-Rothan B,Sánchez-Sevilla J F,Amaya I.Quantitative trait loci and underlying candidate genes controlling agronomical and fruit quality traits in octoploid strawberry(Fragaria×ananassa).Theoretical and Applied Genetics,2012,123(5):755-778
[45]Gaston A,Perrotte J,Lerceteau-K?hler E,Rousseau-Gueutin M,Petit A,Hernould M,Rothan C,Denoyes B.PFRU,a single dominant locus regulates the balance between sexual and asexual plant reproduction in cultivated strawberry.Journal of Experimental Botany,2013,64(7):1837-1848
[46]Perrotte J,Gaston A,Potier A,Petit A,Rothan C,Denoyes B.Narrowing down the single homoeologous FaPFRU locus controlling flowering in octoploid strawberry using a selective mapping strategy.Plant Biotechnology Journal,2016,14(11):2176-2189
[47]Verma S,Roach J,Mangandi J,Lee S,Salinas N,Bassil N,Bink M,van de Weg E,Peace C,Iezzoni A,Whitaker V.DNA-informed strawberry breeding in RosBREED//San Diego,CA,USA:Plant and animal genome XXIV conference.[2016-08-13]https://pag.confex.com/pag/xxiv/webprogram/Paper18784.html
[48]Sargent D J,Hadonou A M,Simpson D W.Development and characterization of polymorphic microsatellite markers from Fragaria viridis,a wild diploid strawberry.Molecular Ecology Resources,2003,3(4):550-552
[49]James C M,Wilson F,Hadonou A M,Tobutt K R.Isolation and characterization of polymorphic microsatellites in diploid strawberry(Fragaria vesca L.)for mapping,diversity studies and clone identification.Molecular Ecology Notes,2003,3(2):171-173
[50]Davis T M,DiMeglio L M,Yang R,Styan S M N,Lewers K S.Assessment of SSR marker transfer from the cultivated strawberry to diploid strawberry species:functionality,linkage group assignment,and use in diversity analysis.The Journal of the American Society for Horticultural Science,2006,131(4):506-512
[51]Sargent D J,Passey T,Surbanovski N,Lopez Girona E,Kuchta P,Davik J,Harrison R,Passey A,Whitehouse A B,Simpson D W.A microsatellite linkage map for the cultivated strawberry(Fragaria×ananassa)suggests extensive regions of homozygosity in the genome that may have resulted from breeding and selection.Theoretical and Applied Genetics,2012,124(7):1229-1240
[52]Isobe S N,Hirakawa H,Sato S,Maeda F,Ishikawa M,Mori T,Yamamoto Y,Shirasawa K,Kimura M,Fukami M,Hashizume F,Tsuji T,Sasamoto S,Kato M,Nanri K,Tsuruoka H,Minami C,Takahashi C,Wada T,Ono A,Kawashima K,Nakazaki N,Kishida Y,Kohara M,Nakayama S,Yamada M,Fujishiro T,Watanabe A,Tabata S.Construction of an integrated high density simple sequence repeat linkage map in cultivated strawberry(Fragaria×ananassa)and its applicability.DNAResearch,2013,20(1):79-92
[53]Sánchez-Sevilla J F,Horvath A,Botella M A,Gaston A,Folta K,Kilian A,Denoyes B,Amaya I.Diversity arrays technology(DArT)marker platforms for diversity analysis and linkage mapping in a complex crop,the octoploid cultivated strawberry(Fragaria×ananassa).PLoS One,2015,10(12):e0144960
[54]Noh Y H,Lee S,Whitaker V M,Cearley K R,Cha J S.Ahigh-throughput marker-assisted selection system combining rapid DNA extraction and high-resolution melting and simple sequence repeat analysis:strawberry as a model for fruit crops.Journal of Berry Research,2017,7(1):23-31
[55]Lerceteau-K?hler E,Guérin G,Denoyes-Rothan B.Identification of SCAR markers linked to Rca2 anthracnose resistance gene and their assessment in strawberry germplasm.Theoretical and Applied Genetics,2005,111(5):862-870
[56]Mathey M M.Phenotyping diverse strawberry(Fragaria spp.)germplasm for aid in marker-assisted breeding,and marker-trait association for red stele(Phytophthora fragariae)resistance marker Rpf1.Oregon:Oregon State University,USA,2013
[57]Salinas N.Validation of molecular markers associated with perpetual flowering(PF)and soluble solids content(SSC)in strawberry(Fragaria×ananassa Duch.ex Rozier).Oregon:Oregon State University,USA,2015
[58]Meuwissen T H E,Hayes B J,Goddard M E.Prediction of total genetic value using genome-wide dense marker maps.Genetics,2001,157(4):1819-1829
[59]Gezan S,Osorio L F,Verma S,Whitaker V M.An experimental validation of genomic selection in octoploid strawberry.Horticulture Research,2017,4:16070
[60]Asker S.Some viewpoints on Fragaria×Potentilla intergeneric hybridization.Hereditas,1971,67(2):181-190