右美托咪定预处理对CLP大鼠急性肾损伤的保护作用
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摘要
目的观察右美托咪定预处理对盲肠结扎穿孔(CLP)脓毒症模型大鼠急性肾损伤的影响及TLR4表达变化。方法 24只大鼠随机分为三组,假手术(SHAM)组,模型(CLP)组和右美托咪定(DEX)组,每组8只,各组在造模前30min分别腹腔注射等剂量生理盐水或右美托咪定100μg·kg~(-1),随后行CLP造模并观察24h,酶联免疫吸附法测定血清TNF-α和IL-6,HE染色观察肾组织病理结构变化,荧光定量PCR测定肾组织TLR4 mRNA表达。结果与CLP组比较,DEX组血清TNF-α和IL-6含量明显减少(P<0.01),肾组织的损伤情况明显减轻,TLR4 mRNA表达亦显著减少(P<0.01)。结论右美托咪定预处理能减轻脓毒症引起的肾组织炎症反应,具有一定保护作用。
OBJECTIVE To observe the effects of dexmedetomidine preconditioning on renal acute injury and the TLR4 expression changes in rats with cecal ligation perforation( CLP). METHODS 24 rats were randomly divided into three groups,SHAM group,model( CLP) group and dexmedetomidine( DEX) group,each group includes eight rats,and recerved isodose physiological saline or dexmedetomidine( 100μg·kg-1) by intraperitoneal respectively 30 minutes before making model,then makes the CLP building 24 h and observes it. TNF-α and IL-6 in the serum were measured by ELISA. The changes of pathological structure of renal tissue were observed with hematoxylin-eosin staining and the expression of renal TLR4 mR NA was measured by RT PCR. RESULTS Compared with group CLP,the expression of TNF-α and IL-6 in the serum significantly decreased( P < 0. 01),the change of kidney his-tological showed lessened injury,the level of TLR4 mR NA was lower apparently( P < 0. 01) in group DEX. CONCLUSION The pretreatment of dexmedetomidine can reduce the inflammatory reaction of nephridial tissue caused by sepsis and have a certain protective effect.
OBJECTIVE To observe the effects of dexmedetomidine preconditioning on renal acute injury and the TLR4 expression changes in rats with cecal ligation perforation( CLP). METHODS 24 rats were randomly divided into three groups,SHAM group,model( CLP) group and dexmedetomidine( DEX) group,each group includes eight rats,and recerved isodose physiological saline or dexmedetomidine( 100μg·kg-1) by intraperitoneal respectively 30 minutes before making model,then makes the CLP building 24 h and observes it. TNF-α and IL-6 in the serum were measured by ELISA. The changes of pathological structure of renal tissue were observed with hematoxylin-eosin staining and the expression of renal TLR4 mR NA was measured by RT PCR. RESULTS Compared with group CLP,the expression of TNF-α and IL-6 in the serum significantly decreased( P < 0. 01),the change of kidney his-tological showed lessened injury,the level of TLR4 mR NA was lower apparently( P < 0. 01) in group DEX. CONCLUSION The pretreatment of dexmedetomidine can reduce the inflammatory reaction of nephridial tissue caused by sepsis and have a certain protective effect.
引文
[1]于冬梅,张铁铮,周锦,等.右美托咪定对内毒素血症大鼠急性肾损伤的保护作用[J].实用药物与临床,2014,17(12):1545-1547.
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[5]Y.Chen,L.Miao,Y.Yao,et al.Dexmedetomidine Ameliorate CLP-Induced Rat Intestinal Injury via Inhibition of Inflammation[J].Mediators Inflamm,2015,(7):918361.
[6]J.Zhang,Z.Wang,Y.Wang,et al.The effect of dexmedetomidine on inflammatory response of septic rats[J].BMC Anesthesiology,2015,15(1):68.
[2]D.Rittirsch MS,Huber-Lang MA,Flierl PA.Ward.Immunodesign of experimental sepsis by cecal ligation and puncture[J].Nature protocols,2009,4(1):31-36.
[3]林峰,吴伟芳,杨瑞芝,等.盲肠结扎穿孔方式与小鼠死亡情况的相关性研究[J].海峡药学,2017,29(6):258-260.
[4]方传奇,李建中.右美托咪定对脂多糖致伤大鼠肾小管上皮细胞TOLL样受体4表达的影响[J].江苏医药,2016,42(2):137-139.
[5]Y.Chen,L.Miao,Y.Yao,et al.Dexmedetomidine Ameliorate CLP-Induced Rat Intestinal Injury via Inhibition of Inflammation[J].Mediators Inflamm,2015,(7):918361.
[6]J.Zhang,Z.Wang,Y.Wang,et al.The effect of dexmedetomidine on inflammatory response of septic rats[J].BMC Anesthesiology,2015,15(1):68.