卡松对柳州白莲洞遗址表面霉菌的抑制作用
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摘要
本文主要探究了白莲洞遗址表面的霉菌的种类,以及卡松对分离的霉菌的抑制作用.将在遗址表面采集的土样配制成土悬液,采用平板涂布法在马铃薯葡萄糖琼脂培养基上培养霉菌并分离纯化得到6种主要的霉菌,分别为地霉属、根霉属、青霉属、毛霉属、曲霉属(黄曲霉、黑曲霉).用滤纸片法考察不同浓度的卡松溶液对霉菌的抑制作用,发现对曲霉、地霉、根霉、青霉的抑菌效果良好,时效长,对毛霉的抑菌效果相对较差.
In this paper, the types of molds from the surface of Bailiandong site and the inhibitory effect of kathon on the isolated molds were studied. The collected soils were prepared into soil suspension.The molds contained in suspension were cultured on potato dextrose agar medium by spread plate method, and then purified for several times. Finally, six kinds of major molds were isolated. They were geotrichum, rhizopus, penicillum, mucor, aspergillus(aspergillus flavus and aspergillus niger),respectively. The inhibitory effect of different concentrations of kathon solution on mold was studied by filter paper method. The result indicated that the antibacterial effect on aspergillus, geotrichum,rhizopus, penicillum is good and preserves for a long time, but that on mucor is worse.
In this paper, the types of molds from the surface of Bailiandong site and the inhibitory effect of kathon on the isolated molds were studied. The collected soils were prepared into soil suspension.The molds contained in suspension were cultured on potato dextrose agar medium by spread plate method, and then purified for several times. Finally, six kinds of major molds were isolated. They were geotrichum, rhizopus, penicillum, mucor, aspergillus(aspergillus flavus and aspergillus niger),respectively. The inhibitory effect of different concentrations of kathon solution on mold was studied by filter paper method. The result indicated that the antibacterial effect on aspergillus, geotrichum,rhizopus, penicillum is good and preserves for a long time, but that on mucor is worse.
引文
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[2]柳州市博物馆.柳州市白莲洞旧石器时代晚期文化遗址中的脊椎动物遗骸[J].古脊椎动物与古人类,1975,13(2):137.
[3]刘文.柳州白莲洞、鲤鱼嘴遗址燧石石器[J].四川文物,2008(6):36-38.
[4]周国兴.中国广西柳州白莲洞石器时代洞穴遗址--对华南地区旧石器时代晚期文化向新石器时代早期文化过渡的探索[J].东南文化,1986(2):8-13.
[5]苏文俊,王金海,付敏,等.白莲洞遗址危岩体形成机理及破坏模式[J].煤田地质与勘探,2016,44(2):59-65,72.
[6]夏寅,李蔓,张尚欣,等.遗址博物馆内土遗址本体可溶性盐和霉菌危害预防与治理的进展[J].文物保护与考古科学,2013,25(4):114-119.
[7]田金英,王春蕾.霉菌对文物的影响初探[J].中国博物馆,1999(1):82-86.
[8]李晓华.试谈霉菌对文物的危害及防治[J].东南文化,1996(3):134-137.
[9]徐卫国,陈勇.2-甲基-4-异噻唑啉-3-酮及其衍生物5-氯-2-甲基-4-异噻唑啉-3-酮的合成[J].浙江化工,2001,32(2):55-57.
[10]李程碑,解彬.异噻唑啉酮(卡松)防腐剂的色泽及工艺控制[J].应用化工,2012,41(2):286-289,293.
[11]武婷,王超,王星,等.反相高效液相色谱法测定化妆品中的24种防腐剂[J].分析化学,2007,35(10):1439-1443.
[12]魏景超.真菌鉴定手册[M].上海:上海科学技术出版社,1979.
[13]中华人民共和国卫生部.食品卫生微生物学检验常见产毒霉菌的鉴定:GB/T 4789.16-2003[S].北京:中国标准出版社,2003.
[14]戴芳澜.真菌的形态和分类[M].北京:科学出版社,1987.
[15]张文康,梁燕艺,刘瑶,等.CdS/g-C3N4复合材料的制备及其光催化性能研究[J].广西科技大学学报,2018,29(4):66-73.
[16]罗春林,韦菲,侯蕾,等.T8-NH2/E-44复合涂层的制备及其防腐性能研究[J].广西科技大学学报,2016,27(3):50-54.