中哈边境区域黑腿星翅蝗遗传多样性与遗传分化研究
|
摘要
【目的】为了明确中哈边境区域黑腿星翅蝗Calliptamus barbarus Costa的遗传多样性,阐明其区域性发生的遗传机制。【方法】测定中哈边境新疆边境区域阿勒泰、塔城、博乐、伊犁等和哈萨克斯坦境内黑腿星翅蝗5个种群100个体的COⅠ基因全长序列(1 540 bp),利用DnaSP 5.0,MEGA 6.0和Arlequin3.5等软件分析种群遗传多样性与分化情况。【结果】共获得100条COⅠ基因序列,在5个地理种群中发现122个变异位点,包含69个单倍型,其中1个单倍型为4个种群所共享。种群间的遗传距离范围为0.001-0.086。总群体单倍型多样性指数H_d=0.987,各地理种群单倍型多样性介于0.973-0.995之间,总群体核苷酸多样性P_i=0.008 4,总群体的遗传分化系数G_(st)=0.004 5,总群体固定系数F_(st)=0.014 2,总基因流N_m=4.61。中性检验(Tajima's D=-1.553 3,P>0.05;Fu’s Fs=-3.732 4,P>0.05)结果表明中哈边境黑腿星翅蝗种群在较近的历史上没有出现群体扩张。单倍型网络图和NJ单倍型系统树结果一致,博乐种群与其他种群分化较明显,AMOVA分子变异分析结果表明中哈边境不同地理种群黑腿星翅蝗的遗传分化主要来自种群内部(98.56%),种群间变异水平很低(1.44%)。不同地理种群的遗传距离与地理距离间的相关性不显著。各单倍型散布在不同地理种群中,未形成明显的系统地理结构。【结论】中哈边境区域不同地理种群黑腿星翅蝗基因交流频繁,遗传分化程度低。
[Objectives] To investigate the genetic diversity of Calliptamus barbarus in the border area between China and Kazakhstan, and reveal the molecular mechanism underlying serious regional outbreaks of this pest. [Methods] Specimens of C. barbarus were collected from 5 different geographic populations(ALT, TC, BL, YL and Kazakhstan) on the border between China and Kazakhstan. A total of 100 full-length sequences of the mitochondrial cytochrome oxidase subunit Ⅰ(COⅠ)gene(1 540 bp) were obtained, sequenced, and compared, using DnaSP 5.0 and Arlequin 3.5 software. [Results] About 122 mutations were found, including 69 haplotypes, one of which was shared by all 4 populations. The genetic distance between populations ranged from 0.001 to 0.086 and the total haplotype diversity index(H_d) was 0.987. The diversity of haplotypes among different geographic populations ranged from 0.973 to 0.995. The total nucleotide diversity(P_i), total genetic differentiation coefficient(G_(st)), total fixed coefficient(F_(st)), and total gene flow(N_m) were 0.008 4, 0.004 5, 0.014 2 and 4.61,respectively. Neutrality test results(Tajima′s D=-1.553 3,P >0.05;Fu's Fs=-3.732 4,P >0.05) indicated that C. barbarus populations on the border between China and Kazakhstan have not recently expanded. The information obtained from the haplotype network and a neighbour-joining phylogenetic tree were consistent, and the differentiation of the BL population from other populations was obvious. The genetic difference between populations(1.44%) is much less than the genetic variation within populations, indicating that geographic distance has no obvious effect on the genetic distance between different populations. No obvious geographic structure is apparent in a phylogenetic tree and a haplotype network based on haplotypes of the different geographic populations. [Conclusion] There is frequent gene exchange between different geographic populations of C. barbarus in the border area between China and Kazakhstan and genetic differentiation of this species in this region is consequently low.
[Objectives] To investigate the genetic diversity of Calliptamus barbarus in the border area between China and Kazakhstan, and reveal the molecular mechanism underlying serious regional outbreaks of this pest. [Methods] Specimens of C. barbarus were collected from 5 different geographic populations(ALT, TC, BL, YL and Kazakhstan) on the border between China and Kazakhstan. A total of 100 full-length sequences of the mitochondrial cytochrome oxidase subunit Ⅰ(COⅠ)gene(1 540 bp) were obtained, sequenced, and compared, using DnaSP 5.0 and Arlequin 3.5 software. [Results] About 122 mutations were found, including 69 haplotypes, one of which was shared by all 4 populations. The genetic distance between populations ranged from 0.001 to 0.086 and the total haplotype diversity index(H_d) was 0.987. The diversity of haplotypes among different geographic populations ranged from 0.973 to 0.995. The total nucleotide diversity(P_i), total genetic differentiation coefficient(G_(st)), total fixed coefficient(F_(st)), and total gene flow(N_m) were 0.008 4, 0.004 5, 0.014 2 and 4.61,respectively. Neutrality test results(Tajima′s D=-1.553 3,P >0.05;Fu's Fs=-3.732 4,P >0.05) indicated that C. barbarus populations on the border between China and Kazakhstan have not recently expanded. The information obtained from the haplotype network and a neighbour-joining phylogenetic tree were consistent, and the differentiation of the BL population from other populations was obvious. The genetic difference between populations(1.44%) is much less than the genetic variation within populations, indicating that geographic distance has no obvious effect on the genetic distance between different populations. No obvious geographic structure is apparent in a phylogenetic tree and a haplotype network based on haplotypes of the different geographic populations. [Conclusion] There is frequent gene exchange between different geographic populations of C. barbarus in the border area between China and Kazakhstan and genetic differentiation of this species in this region is consequently low.
引文
Azhbenov VK,Baybussenov KS,Sarbaev AT,Harizanova VB,2015.Preventive approach of phytosanitary control of locust pests in Kazakhstan and adjacent areas.International Conference on Agricultural,Ecological and Medical Sciences.Pengang,Malaysia.33-37.
Baybussenov KS,Sarbaev AT,Azhbenov VK,Harizanova VB,2014.Environmental features of population dynamics of hazard nongregarious locusts in northern Kazakhstan.Life Science Journal,11(10s):277-281.
Baybussenov KS,Sarbaev AT,Azhbenov VK,Harizanova VB,2015.Predicting the phase state of the abundance dynamics of harmful non-gregarious locusts in Northern Kazakhstan and substantiation of protective measures.Biosciences Biotechnology Research Asia,12(2):1535-1543.
Bensasson D,Zhang DX,Hewitt GM,2000.Frequent assimilation of mitochondrial DNA by grasshopper nuclear genomes.Molecular Biology and Evolution,17(3):406-415.
Blanchet E,Blondin L,Gagnaire PA,Foucart A,Vassal JM,Lecoq M,2010.Multiplex PCR assay to discriminate four neighbour species of the Calliptamus genus(Orthoptera:Acrididae)from France.Bulletin of Entomology and Research,100(6):701-706.
Blanchet E,Lecoq M,Sword GA,Pages C,Blondin L,Billot C,Rivallan R,Foucart A,Vassal JM,Risterucci AM,Chapuis MP,2012a.Population structures of three Calliptamus spp.(Orthoptera:Acrididae)across the Western Mediterranean Basin.European Journal of Entomology,109(3):445-455.
Blanchet E,Lecoq M,Pages C,Rivallan R,Foucart A,Billot C,Vassal JM,Risterucci AM,Chapuis MP,2012b.A comparative analysis of fine-scale genetic structure in three closely related syntopic species of the grasshopper genus Calliptamus.Canadian Journal of Zoology-Revue Canadienne de Zoologie,90(1):31-41.
Burgov A,Novikova O,Mayorov V,Adkison L,Blinov A,2006.Molecular phylogeny of Palearctic genera of Gomphocerinae grasshoppers(Orthoptera:Acrididae).Systematic Entomology,31(2):362-368.
Chen T,Chen HW,Yang L,2013.The Climate productivity response to the regional climate changes in Bortala Mongol Autonomous Prefecture of China.Desert and Oasis Meteorology,7(3):57-61.[陈涛,陈洪武,杨辽,2013.博州地区气候生产力对区域气候变化的响应.沙漠与绿洲气象,7(3):57-61.]
Gao SJ,Li DW,Liu AP,Yan ZJ,Xu LB,2011.Sequence analysis of mt DNA COⅠgene and relationships of geographical populations of Oedaleus asiaticu in Inner Mongolia.Acta Agrestia Sinica,19(5):846-857.[高书晶,李东伟,刘爱萍,闫志坚,徐林波,2011.不同地理种群的亚洲小车蝗mt DNA COⅠ基因序列及其相互关系.草地学报,19(5):846-857.]
Huo GM,2006.Molecular phylogenetic analysis of acridoidea from COⅠand Cytb gene sequences.Master thesis.Nanjing:Nanjing Normal University.[霍光明,2006.应用COⅠ和Cytb基因序列研究蝗总科昆虫的系统进化.硕士学位论文.南京:南京师范大学.]
Jiang SS,2011.Study on genetic diversity of Rammeacris kiangsu of 5 geographical populations based on partial sequences of 16Sr RNA gene,COⅠgene,and COⅠⅠgene.Master thesis.Changsha:Central South University of Forestry and Technology.[姜石生,2011.基于线粒体DNA的16S r RNA、COⅠ和COⅠⅠ基因的黄脊竹蝗5地理种群遗传多样性研究.硕士学位论文.长沙:中南林业科技大学.]
Kalinowski ST,2005.Do polymorphic loci require large sample sizes to estimate genetic distances?Heredity,94(1):33-36.
Kokanova EO,2014.The current state of natural foci of swarming locusts(Orthoptera,Acrididae)in Turkmenistan.Entomological Review,94(6):826-828.
Leigh JW,Bryant D,2015.Pop ART:Full-feature software for haplotype network construction.Methods in Ecology and Evolution,6(9):1110-1116.
Li YL,Li X,Liang TS,Zhang LG,Li DD,2013.Sequence analysis of mt DNA COⅠgene of 7 geographical populations of Oedaleus asiaticus in Beijing and surrounding regions.Plant Protection,39(5):117-222.[李云龙,李霞,梁铁双,张立国,李冬冬,2013.北京及周边地区7个地理种群的亚洲小车蝗mt DNA COⅠ基因序列分析.植物保护,39(5):117-222.]
Lu Y,Wang HQ,Wei XZ,Chen R,Han ST,Li J,2013.Characteristics and causes of Loxostege sticticalis reappearing in Xinjiang in 2012.China Plant Protection,33(12):47-51.[芦屹,王惠卿,魏新政,陈蓉,韩顺涛,李晶,2013.2012年新疆草地螟重发特点及原因分析.中国植保导刊,33(12):47-51.]
Librado P,Rozas J,2009.Dna SP v5:a software for comprehensive analysis of DNA polymorphism data.Bioinformatics,25(11):1451-1452.
Ma C,Yang PC,Jiang F,Marie-Pierre C,Yasen S,Gregory AS,Kang L,2012.Mitochondrial genomes reveal the global phylogeography and dispersal routes of the migratory locust.Molecular Ecology,21(17):4344-4358.
Rouibah M,López-López A,Presa JJ,Doumandji SA,2016.Molecular phylogenetic and phylogeographic study of two forms of Calliptamus barbarus(Costa 1836)(Orthoptera:Acrididae,Calliptaminae)from two regions of Algeria.International Journal of Entomology,52(2):77-87.
Rozas J,Sánchez-Delbarrio JC,Messeguer X,Rozas R,2003.Dna SP,DNA polymorphism analyses by the coalescent and other methods.Bioinformatics,19(18):2496-2497.
Schneider S,Roessli D,Exco EL,2000.Arlequin(version 2000):Asoftware for population genetic analysis.Geneva:Genetics and Biometry Laboratory,University of Geneva.
Sergeev MG,1992.Distribution patterns of Orthoptera in North and Central Asia.Journal of Orthoptera Research,182(1):14-24.
Sun W,Zhang ZT,Lei CP,Dong H,Qian HT,Cong B,2013.Genetic diversity and genetic differentiation among different geographic populations of Oedaleus infernalis Saussure(Orthoptera:Acridoidea).Journal of Shenyang Agricultural University,44(6):748-753.[孙嵬,张柱亭,类成平,董辉,钱海涛,丛斌,2013.不同地理种群黄胫小车蝗的遗传多样性及遗传分化研究.沈阳农业大学学报,44(6):748-753.]
Tamura K,Stecher G,Peterson D,Filipski A,Kumar S,2013.MEGA6:molecular evolutionary genetics analysis verson 6.0.Molecular Biology and Evolution,30:2725-2729.
Tajima F,1989.Statistical method for testing the neutral mutation hypothesis by DNA polymorphism.Genetics,123(3):585-595.
Wang L,Xu GQ,Liu DF,Han XL,2006.A study of the relation between a siatic migratory locust and meteorological factors.Xinjiang Meteorology,29(5):25-27.[王磊,徐光青,刘大锋,韩晓玲,2006.迁入性亚洲飞蝗与气象因子关系的研究.新疆气象,29(5):25-27.]
Xu MY,2009.Molecular phylogeny of Acridoidea basedon 18Sr RNA,16S r RNA,COⅠ,COⅠⅠgenes.Master thesis.Baoding:Hebei University.[徐淼洋,2009.基于18S r RNA、16S r RNA、COⅠ、COⅠⅠ基因的蝗总科系统发育研究.硕士学位论文.保定:河北大学.]
Yang L,2008.Molecular evolution and phylogeny of mitochondrial COⅠand COⅠⅠgenes in Catantopidae.Master thesis.Xi’an:Shanxi Normal University.[杨亮,2008.斑腿蝗科部分种的线粒体COⅠ与COⅠⅠ基因分子进化与系统学研究.硕士学位论文.西安:陕西师范大学.]
Yang XM,Lu YH,2018.Population genetic structure of Helicoverpa armigera in Ningxia,Inner Mongolia and neighboring areas.Chinese Journal of Applied Entomology,55(1):25-31.[杨现明,陆宴辉,2018.基于线粒体DNA的宁夏、内蒙古及周边地区棉铃虫种群遗传结构.应用昆虫学报,55(1):25-31.]
Zhang L,2008.Molecular phylogeny of Catantopidae of COⅠand Cytb sequences.Master thesis.Xi’an:Shanxi Normal University.[张陵,2008.基于COⅠ与Cytb基因序列的斑腿蝗科部分种类的分子系统学研究.硕士学位论文.西安:陕西师范大学.]
Zhang LJ,Luo JY,Zhang S,Ma Y,Wang CY,Lv LM,Zhu CZ,Cui JJ,2018.Population genetic structure and genetic diversity of Adelphocoris nigritylus based on mt DNA COⅠgene sequence variation.Chinese Journal of Applied Entomology,55(4):667-678.[张丽娟,雒珺瑜,张帅,马妍,王春义,吕丽敏,朱香镇,崔金杰,2018.基于线粒体COⅠ基因黑唇苜蓿盲蝽种群遗传结构与遗传多样性分析.应用昆虫学报,55(4):667-678.]
Baybussenov KS,Sarbaev AT,Azhbenov VK,Harizanova VB,2014.Environmental features of population dynamics of hazard nongregarious locusts in northern Kazakhstan.Life Science Journal,11(10s):277-281.
Baybussenov KS,Sarbaev AT,Azhbenov VK,Harizanova VB,2015.Predicting the phase state of the abundance dynamics of harmful non-gregarious locusts in Northern Kazakhstan and substantiation of protective measures.Biosciences Biotechnology Research Asia,12(2):1535-1543.
Bensasson D,Zhang DX,Hewitt GM,2000.Frequent assimilation of mitochondrial DNA by grasshopper nuclear genomes.Molecular Biology and Evolution,17(3):406-415.
Blanchet E,Blondin L,Gagnaire PA,Foucart A,Vassal JM,Lecoq M,2010.Multiplex PCR assay to discriminate four neighbour species of the Calliptamus genus(Orthoptera:Acrididae)from France.Bulletin of Entomology and Research,100(6):701-706.
Blanchet E,Lecoq M,Sword GA,Pages C,Blondin L,Billot C,Rivallan R,Foucart A,Vassal JM,Risterucci AM,Chapuis MP,2012a.Population structures of three Calliptamus spp.(Orthoptera:Acrididae)across the Western Mediterranean Basin.European Journal of Entomology,109(3):445-455.
Blanchet E,Lecoq M,Pages C,Rivallan R,Foucart A,Billot C,Vassal JM,Risterucci AM,Chapuis MP,2012b.A comparative analysis of fine-scale genetic structure in three closely related syntopic species of the grasshopper genus Calliptamus.Canadian Journal of Zoology-Revue Canadienne de Zoologie,90(1):31-41.
Burgov A,Novikova O,Mayorov V,Adkison L,Blinov A,2006.Molecular phylogeny of Palearctic genera of Gomphocerinae grasshoppers(Orthoptera:Acrididae).Systematic Entomology,31(2):362-368.
Chen T,Chen HW,Yang L,2013.The Climate productivity response to the regional climate changes in Bortala Mongol Autonomous Prefecture of China.Desert and Oasis Meteorology,7(3):57-61.[陈涛,陈洪武,杨辽,2013.博州地区气候生产力对区域气候变化的响应.沙漠与绿洲气象,7(3):57-61.]
Gao SJ,Li DW,Liu AP,Yan ZJ,Xu LB,2011.Sequence analysis of mt DNA COⅠgene and relationships of geographical populations of Oedaleus asiaticu in Inner Mongolia.Acta Agrestia Sinica,19(5):846-857.[高书晶,李东伟,刘爱萍,闫志坚,徐林波,2011.不同地理种群的亚洲小车蝗mt DNA COⅠ基因序列及其相互关系.草地学报,19(5):846-857.]
Huo GM,2006.Molecular phylogenetic analysis of acridoidea from COⅠand Cytb gene sequences.Master thesis.Nanjing:Nanjing Normal University.[霍光明,2006.应用COⅠ和Cytb基因序列研究蝗总科昆虫的系统进化.硕士学位论文.南京:南京师范大学.]
Jiang SS,2011.Study on genetic diversity of Rammeacris kiangsu of 5 geographical populations based on partial sequences of 16Sr RNA gene,COⅠgene,and COⅠⅠgene.Master thesis.Changsha:Central South University of Forestry and Technology.[姜石生,2011.基于线粒体DNA的16S r RNA、COⅠ和COⅠⅠ基因的黄脊竹蝗5地理种群遗传多样性研究.硕士学位论文.长沙:中南林业科技大学.]
Kalinowski ST,2005.Do polymorphic loci require large sample sizes to estimate genetic distances?Heredity,94(1):33-36.
Kokanova EO,2014.The current state of natural foci of swarming locusts(Orthoptera,Acrididae)in Turkmenistan.Entomological Review,94(6):826-828.
Leigh JW,Bryant D,2015.Pop ART:Full-feature software for haplotype network construction.Methods in Ecology and Evolution,6(9):1110-1116.
Li YL,Li X,Liang TS,Zhang LG,Li DD,2013.Sequence analysis of mt DNA COⅠgene of 7 geographical populations of Oedaleus asiaticus in Beijing and surrounding regions.Plant Protection,39(5):117-222.[李云龙,李霞,梁铁双,张立国,李冬冬,2013.北京及周边地区7个地理种群的亚洲小车蝗mt DNA COⅠ基因序列分析.植物保护,39(5):117-222.]
Lu Y,Wang HQ,Wei XZ,Chen R,Han ST,Li J,2013.Characteristics and causes of Loxostege sticticalis reappearing in Xinjiang in 2012.China Plant Protection,33(12):47-51.[芦屹,王惠卿,魏新政,陈蓉,韩顺涛,李晶,2013.2012年新疆草地螟重发特点及原因分析.中国植保导刊,33(12):47-51.]
Librado P,Rozas J,2009.Dna SP v5:a software for comprehensive analysis of DNA polymorphism data.Bioinformatics,25(11):1451-1452.
Ma C,Yang PC,Jiang F,Marie-Pierre C,Yasen S,Gregory AS,Kang L,2012.Mitochondrial genomes reveal the global phylogeography and dispersal routes of the migratory locust.Molecular Ecology,21(17):4344-4358.
Rouibah M,López-López A,Presa JJ,Doumandji SA,2016.Molecular phylogenetic and phylogeographic study of two forms of Calliptamus barbarus(Costa 1836)(Orthoptera:Acrididae,Calliptaminae)from two regions of Algeria.International Journal of Entomology,52(2):77-87.
Rozas J,Sánchez-Delbarrio JC,Messeguer X,Rozas R,2003.Dna SP,DNA polymorphism analyses by the coalescent and other methods.Bioinformatics,19(18):2496-2497.
Schneider S,Roessli D,Exco EL,2000.Arlequin(version 2000):Asoftware for population genetic analysis.Geneva:Genetics and Biometry Laboratory,University of Geneva.
Sergeev MG,1992.Distribution patterns of Orthoptera in North and Central Asia.Journal of Orthoptera Research,182(1):14-24.
Sun W,Zhang ZT,Lei CP,Dong H,Qian HT,Cong B,2013.Genetic diversity and genetic differentiation among different geographic populations of Oedaleus infernalis Saussure(Orthoptera:Acridoidea).Journal of Shenyang Agricultural University,44(6):748-753.[孙嵬,张柱亭,类成平,董辉,钱海涛,丛斌,2013.不同地理种群黄胫小车蝗的遗传多样性及遗传分化研究.沈阳农业大学学报,44(6):748-753.]
Tamura K,Stecher G,Peterson D,Filipski A,Kumar S,2013.MEGA6:molecular evolutionary genetics analysis verson 6.0.Molecular Biology and Evolution,30:2725-2729.
Tajima F,1989.Statistical method for testing the neutral mutation hypothesis by DNA polymorphism.Genetics,123(3):585-595.
Wang L,Xu GQ,Liu DF,Han XL,2006.A study of the relation between a siatic migratory locust and meteorological factors.Xinjiang Meteorology,29(5):25-27.[王磊,徐光青,刘大锋,韩晓玲,2006.迁入性亚洲飞蝗与气象因子关系的研究.新疆气象,29(5):25-27.]
Xu MY,2009.Molecular phylogeny of Acridoidea basedon 18Sr RNA,16S r RNA,COⅠ,COⅠⅠgenes.Master thesis.Baoding:Hebei University.[徐淼洋,2009.基于18S r RNA、16S r RNA、COⅠ、COⅠⅠ基因的蝗总科系统发育研究.硕士学位论文.保定:河北大学.]
Yang L,2008.Molecular evolution and phylogeny of mitochondrial COⅠand COⅠⅠgenes in Catantopidae.Master thesis.Xi’an:Shanxi Normal University.[杨亮,2008.斑腿蝗科部分种的线粒体COⅠ与COⅠⅠ基因分子进化与系统学研究.硕士学位论文.西安:陕西师范大学.]
Yang XM,Lu YH,2018.Population genetic structure of Helicoverpa armigera in Ningxia,Inner Mongolia and neighboring areas.Chinese Journal of Applied Entomology,55(1):25-31.[杨现明,陆宴辉,2018.基于线粒体DNA的宁夏、内蒙古及周边地区棉铃虫种群遗传结构.应用昆虫学报,55(1):25-31.]
Zhang L,2008.Molecular phylogeny of Catantopidae of COⅠand Cytb sequences.Master thesis.Xi’an:Shanxi Normal University.[张陵,2008.基于COⅠ与Cytb基因序列的斑腿蝗科部分种类的分子系统学研究.硕士学位论文.西安:陕西师范大学.]
Zhang LJ,Luo JY,Zhang S,Ma Y,Wang CY,Lv LM,Zhu CZ,Cui JJ,2018.Population genetic structure and genetic diversity of Adelphocoris nigritylus based on mt DNA COⅠgene sequence variation.Chinese Journal of Applied Entomology,55(4):667-678.[张丽娟,雒珺瑜,张帅,马妍,王春义,吕丽敏,朱香镇,崔金杰,2018.基于线粒体COⅠ基因黑唇苜蓿盲蝽种群遗传结构与遗传多样性分析.应用昆虫学报,55(4):667-678.]