两种合成活性肽对肉鸡免疫功能的影响研究
|
摘要
本试验旨在研究两种合成活性肽对肉鸡免疫功能的影响。1440只1 d AA肉鸡随机分为8个处理,设置2个对照组(空白对照组及杆菌肽锌组),试验组饮水供给两种肽(均设置3个剂量:10、20、40μg/kg体重),每组设6个重复,试验期42 d。结果表明:40μg/kg体重肽I和肽Ⅱ可显著提高21 d肉鸡血清新城疫抗体效价(P<0.05),显著提高21、42 d鸡血清Ig G水平(P<0.05);3个剂量肽I可显著提高血清Ig M水平(P<0.05),40μg/kg体重肽Ⅱ对42 d鸡亦有此作用(P<0.05)。21 d时各试验组血清IL-2含量均显著高于两个对照组(P<0.05或P<0.01),42 d时40μg/kg体重肽I组和肽Ⅱ组血清IL-2及IFN-r含量显著提高(P<0.01或P<0.05)。40μg/kg体重肽I和肽Ⅱ可显著提高42 d鸡血清溶菌酶含量(P<0.05),40μg/kg体重肽I可显著21 d鸡血清补体C3含量(P<0.05)。20μg/kg体重肽Ⅱ和肽I分别显著提高了21 d鸡法氏囊指数和42 d鸡胸腺指数(P<0.05)。可见,2种合成活性肽均有提高肉鸡免疫功能活性,均以饮水供给40μg/kg体重效果最好。
Two synthetic active peptides were studied to investigate their effe ct on immune function of broilers in this experiment.1440 1-day-old AA broilers were randomly divided into eight groups including two control groups(blank control group and bacitracin zinc group) and trial groups.Experimental groups with 6 replicates in each group were given two peptides by drinking supply with 3 doses(10,20,40 μg/kg body weight).The test lasted 42 d.The results showed that:The serum Newcastle disease antibody titers of 21 d broilers were increased significantly by 40 μg/kg body weight peptide I and peptide Ⅱ(P <0.05),and the serum Ig G levels of 21 d and 42 d broilers were increased significantly(P<0.05).The serum Ig M level was increased significantly by peptide I with three doses(P<0.05 or P<0.01),and 40 μg/kg body weight peptideⅡhad the same effect on 42 d broilers(P<0.05).The serum IL-2 contents of all test groups were significantly higher than that of the two control groups at 21d(P<0.05 or P<0.01).The serum IL-2 and IFN-r contents were also increased significantly in groups of 40 μg/kg body weight peptide I and peptide Ⅱ(P<0.05 or P <0.01).The serum lysozyme content of 42 d broilers was significantly improved by 40 μg/kg of body weight peptide I and peptide Ⅱ(P <0.05).The serum complement C3 content of 21 d broilers was significantly improved by40 μg/kg body weight peptide I(P <0.05).The bursal index of 21 d broilers was increased significantly by 20 μg/kg body weight peptideⅡ(P<0.05),and the thymus index of 42 d broilers was also improved by 20 μg/kg of body weight peptide I(P <0.05).So the conclusion was that the two synthetic peptides had the activity of improving the immune function of broilers,and the best dose of them was 40 μg/kg body weight.
Two synthetic active peptides were studied to investigate their effe ct on immune function of broilers in this experiment.1440 1-day-old AA broilers were randomly divided into eight groups including two control groups(blank control group and bacitracin zinc group) and trial groups.Experimental groups with 6 replicates in each group were given two peptides by drinking supply with 3 doses(10,20,40 μg/kg body weight).The test lasted 42 d.The results showed that:The serum Newcastle disease antibody titers of 21 d broilers were increased significantly by 40 μg/kg body weight peptide I and peptide Ⅱ(P <0.05),and the serum Ig G levels of 21 d and 42 d broilers were increased significantly(P<0.05).The serum Ig M level was increased significantly by peptide I with three doses(P<0.05 or P<0.01),and 40 μg/kg body weight peptideⅡhad the same effect on 42 d broilers(P<0.05).The serum IL-2 contents of all test groups were significantly higher than that of the two control groups at 21d(P<0.05 or P<0.01).The serum IL-2 and IFN-r contents were also increased significantly in groups of 40 μg/kg body weight peptide I and peptide Ⅱ(P<0.05 or P <0.01).The serum lysozyme content of 42 d broilers was significantly improved by 40 μg/kg of body weight peptide I and peptide Ⅱ(P <0.05).The serum complement C3 content of 21 d broilers was significantly improved by40 μg/kg body weight peptide I(P <0.05).The bursal index of 21 d broilers was increased significantly by 20 μg/kg body weight peptideⅡ(P<0.05),and the thymus index of 42 d broilers was also improved by 20 μg/kg of body weight peptide I(P <0.05).So the conclusion was that the two synthetic peptides had the activity of improving the immune function of broilers,and the best dose of them was 40 μg/kg body weight.
引文
[1]张栗,王宇.生物活性肽的功能与制备研究进展[J].郑州牧业工程高等专科学校学报,2013,33(3):20-21.
[2]王竹清,李八方.生物活性肽及其研究进展[J].中国海洋药物杂志,2010,29(2):60-68.
[3]刘海军,乐超银,邵伟,等.生物活性肽研究进展[J].中国酿造,2015,(5):5-7.
[4]张鸿鹏,甘一如.多肽液相分段合成及其进展[J].化学工业与工程,2006,23(3):254-259.
[5]郭鑫.动物免疫学实验教程[M].北京:中国农业大学出版社,2006:61-64.
[6]李秀英.免疫禽流感疫苗HI抗体的试验研究[J].河北畜牧兽医,2004,20(12):19-20.
[7]杨海燕,靳继惠,张传美,等.一种新型复合生物活性肽对SPF鸡抗病力和生长性能的影响[J].黑龙江畜牧兽医,2014,10(上):144-146.
[8]韩雪娇,马改彦,周玉岩,等.抗菌肽对肉鸡生产性能和免疫功能的影响[J].中国畜牧杂志,2014,50(07):70-73.
[9]王静.抗菌肽对肉鸡生产性能和免疫功能的影响研究[D].山东农业大学,2007,6.
[10]文慧,魏时来,张石蕊,等.L-茶氨酸对黄羽肉鸡生产性能和免疫功能的影响[J].动物营养学报,2012,24(10):1946-1954.
[11]张斌.人工合成胸腺五肽对雏鸡免疫功能的影响[D].扬州:扬州大学,2007,6.
[12]李金莲.日粮中添加不同水平抗菌肽对肉鸡生长性能及免疫力的影响[J].黑龙江畜牧兽医,2014,06(上):87-88.
[13]Robert E W Hancock,Gill Dilman.The role of cationic antimicrobial peptides in innate host defences[J].Trends Microbio,2000,8(9):402-409.
[14]李丽.抗菌肽Abaecin制备及其对肉鸡生长及免疫影响的研究[D].内蒙古农业大学,2010,06.
[15]都海明.抗菌脂肽对AA肉鸡生产性能、养分代谢及抗氧化机能的影响[D].南京:南京农业大学,2009,06.
[2]王竹清,李八方.生物活性肽及其研究进展[J].中国海洋药物杂志,2010,29(2):60-68.
[3]刘海军,乐超银,邵伟,等.生物活性肽研究进展[J].中国酿造,2015,(5):5-7.
[4]张鸿鹏,甘一如.多肽液相分段合成及其进展[J].化学工业与工程,2006,23(3):254-259.
[5]郭鑫.动物免疫学实验教程[M].北京:中国农业大学出版社,2006:61-64.
[6]李秀英.免疫禽流感疫苗HI抗体的试验研究[J].河北畜牧兽医,2004,20(12):19-20.
[7]杨海燕,靳继惠,张传美,等.一种新型复合生物活性肽对SPF鸡抗病力和生长性能的影响[J].黑龙江畜牧兽医,2014,10(上):144-146.
[8]韩雪娇,马改彦,周玉岩,等.抗菌肽对肉鸡生产性能和免疫功能的影响[J].中国畜牧杂志,2014,50(07):70-73.
[9]王静.抗菌肽对肉鸡生产性能和免疫功能的影响研究[D].山东农业大学,2007,6.
[10]文慧,魏时来,张石蕊,等.L-茶氨酸对黄羽肉鸡生产性能和免疫功能的影响[J].动物营养学报,2012,24(10):1946-1954.
[11]张斌.人工合成胸腺五肽对雏鸡免疫功能的影响[D].扬州:扬州大学,2007,6.
[12]李金莲.日粮中添加不同水平抗菌肽对肉鸡生长性能及免疫力的影响[J].黑龙江畜牧兽医,2014,06(上):87-88.
[13]Robert E W Hancock,Gill Dilman.The role of cationic antimicrobial peptides in innate host defences[J].Trends Microbio,2000,8(9):402-409.
[14]李丽.抗菌肽Abaecin制备及其对肉鸡生长及免疫影响的研究[D].内蒙古农业大学,2010,06.
[15]都海明.抗菌脂肽对AA肉鸡生产性能、养分代谢及抗氧化机能的影响[D].南京:南京农业大学,2009,06.