4种合成活性肽对小鼠生长性能和免疫机能的影响
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摘要
本试验选用4种合成生物活性肽(肽1、肽2、肽3、肽4分别由15、7、9、7个氨基酸构成,采用固相合成柱手工合成的直链肽)对小鼠进行灌喂饲养试验,旨在探讨其对小鼠的生长性能、免疫机能和器官发育的影响。选取清洁级健康小鼠90只,随机分为9组,每组10只,试验组分别连续灌喂10μg/kg体重和50μg/kg体重剂量的4种活性肽,空白对照组灌喂等量蒸馏水。试验期32d。结果表明:(1)灌喂活性肽15d和32d,小鼠体重、平均日耗料量各试验组之间及与对照组间差异均不显著(P>0.05)。(2)小鼠灌喂活性肽15d,50μg/kg体重肽3能显著提高小鼠血清IgG和IFN-γ含量(P<0.05)。(3)小鼠灌喂活性肽15d,50μg/kg体重肽1组小鼠肝脏系数显著高于10μg/kg体重肽1组(P<0.05),其余各组之间无显著差异(P>0.05);心脏、脾脏和肾脏系数各组之间差异均不显著(P>0.05)。小鼠灌喂活性肽32d时,50μg/kg体重活性肽2、肽3、肽4对小鼠心脏、肝脏和肾脏系数有显著提高作用。综上可见,4种人工合成生物活性肽对小鼠生长性能未见明显影响,对免疫机能和脏器系数有一定的促进作用。
A experiment was conducted to discuss the growth performance,immune organ index and immune function of four synthetic bioactive peptides(peptide 1,peptide 2,peptide 3and peptide 4were consist of 15,7,9and 7amino acids respectively,the linear peptides compound by solid-phase synthesis manual)on mice by gastric perfusion.90 clean health mice were randomly divided into 9groups with 10 mice per group with,the test groups were administered with 10μg/kg and 50μg/kg body weight of four synthetic bioactive peptides,the blank control group was fed with equivalent distilled water.The test period was 32 days.The result showed that,(1)There was no significantly difference on the weight and the average daily feed consumption of the mice feeding synthetic bioactive peptides 15 days and 32 days among the experiment groups and control group(P>0.05).(2)Bioactive peptide 3feeding 15 days 50μg/kg body weight can significantly improve serum IgG andγ-interferon content on mice(P<0.05).(3)On the mice feeding synthetic bioactive peptides 15 days,liver coefficient of the group with 50μg/kg body weight peptide 1was significant higher than group 10μg/kg body weight peptide 1(P<0.05),there was no significant different in the other groups(P>0.05).Cardiac,spleen and kidney coefficients were no significant different in the each group(P>0.05).On the mice feeding synthetic bioactive peptides 32 days,bioactive peptide 2,3and 4feeding 50μg/kg body weight could significantly improve its heart,liver and kidney coefficient(P<0.05).So,four synthetic bioactive peptides had no obvious significance on growth performance,and had certain promoting effect on immune function and organ coefficient of mice.
A experiment was conducted to discuss the growth performance,immune organ index and immune function of four synthetic bioactive peptides(peptide 1,peptide 2,peptide 3and peptide 4were consist of 15,7,9and 7amino acids respectively,the linear peptides compound by solid-phase synthesis manual)on mice by gastric perfusion.90 clean health mice were randomly divided into 9groups with 10 mice per group with,the test groups were administered with 10μg/kg and 50μg/kg body weight of four synthetic bioactive peptides,the blank control group was fed with equivalent distilled water.The test period was 32 days.The result showed that,(1)There was no significantly difference on the weight and the average daily feed consumption of the mice feeding synthetic bioactive peptides 15 days and 32 days among the experiment groups and control group(P>0.05).(2)Bioactive peptide 3feeding 15 days 50μg/kg body weight can significantly improve serum IgG andγ-interferon content on mice(P<0.05).(3)On the mice feeding synthetic bioactive peptides 15 days,liver coefficient of the group with 50μg/kg body weight peptide 1was significant higher than group 10μg/kg body weight peptide 1(P<0.05),there was no significant different in the other groups(P>0.05).Cardiac,spleen and kidney coefficients were no significant different in the each group(P>0.05).On the mice feeding synthetic bioactive peptides 32 days,bioactive peptide 2,3and 4feeding 50μg/kg body weight could significantly improve its heart,liver and kidney coefficient(P<0.05).So,four synthetic bioactive peptides had no obvious significance on growth performance,and had certain promoting effect on immune function and organ coefficient of mice.
引文
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[16]张文博,刘静波,王莹,等.蛋清高F值寡肽对受试小鼠脏器影响的研究[J].食品科学,2008,11:601-604
[17]康苏,夏丽洁,马纪,等.新疆家蚕抗菌肽对小鼠体征指标和脏器组织影响的研究[J].食品工业科技,2013,21:343-347
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[19]陈晓生,温刘发,张辉华,等.饲粮中添加抗菌肽对肉鸭生产性能及免疫器官、内脏器官的影响[J].畜禽业,2005,02:12-13
[2]Trindade F,Amado F,Pinto da Costa J,et al.Salivary peptidomic as a tool to disclose new potential antimicrobial peptides[J].J Proteomics,2014,19
[3]陈文雅,计成,王碧莲.生物活性肽的功能与应用[J].饲料研究,2014,21:38-41
[4]孙永泰.多功能饲料添加剂—生物活性肽[J].江西饲料,2014,05:17-19
[5]Martínez-Augustin O,Rivero-Gutiérrez B,Mascaraque C,et al.Food Derived Bioactive Peptides and Intestinal Barrier Function[J].Int J Mol Sci,2014,15(12):22857-22873
[6]Zhang H,Hu CA,Kovacs-Nolan J,et al.Bioactive dietary peptides and amino acids in inflammatory bowel disease[J].Amino Acids,2014,11,14
[7]孙惠敏.CecropinA-thanatin杂合肽对小鼠生长性能及免疫功能的影响[D].银川:宁夏医科大学,2013
[8]黄金雨,陈开健,鄢阳军.生物活性肽对幼鲤生长性能影响的研究[J].内陆水产,2006,(08):32-34
[9]舒美艳,许国焕,吴月嫦,等.乳源活性肽对罗非鱼生长性能和血清生化指标的影响[J].动物营养学报,2009,(02):219-225
[10]汪官保.植物活性肽对哺乳仔猪生产性能的影响及其促生长机理的研究[D].西宁:青海大学,2007
[11]张晶,李牧,单安山,等.大豆活性肽对14日龄早期断奶仔猪生长性能和甲状腺素分泌的影响[J].东北农业大学学报,2005,(04):467-471
[12]刘丽娜,何东平,张声华.花生多肽对正常小鼠免疫功能的影响[J].中国粮油学报,2009,24(8):88-91
[13]吕凤霞,陆兆新.大豆生理活性物质功能的研究进展[J].食品科技,2001,(5):70
[14]国明明,华欲飞.大豆肽免疫调节作用的研究[J].食品科技,2007,(7):242-244
[15]巢警殳.林蛙皮活性肽对小鼠免疫功能的影响[D].长春:吉林大学,2011
[16]张文博,刘静波,王莹,等.蛋清高F值寡肽对受试小鼠脏器影响的研究[J].食品科学,2008,11:601-604
[17]康苏,夏丽洁,马纪,等.新疆家蚕抗菌肽对小鼠体征指标和脏器组织影响的研究[J].食品工业科技,2013,21:343-347
[18]童辉,栾新红,宁志利,等.玉米蛋白多肽对豁眼鹅屠宰性能及脏器系数的影响[J].中国家禽,2009,10:8-10
[19]陈晓生,温刘发,张辉华,等.饲粮中添加抗菌肽对肉鸭生产性能及免疫器官、内脏器官的影响[J].畜禽业,2005,02:12-13