Ⅰ群4型禽腺病毒卵黄抗体效价与鸡攻毒保护相关性试验
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摘要
取实验室试制的卵黄抗体,用磷酸盐缓冲液稀释成Ⅰ群4型禽腺病毒卵黄抗体琼扩效价分别为12、14和18,取不同效价卵黄抗体接种21和42日龄鸡,24 h后进行攻毒保护试验。结果表明,随着卵黄抗体效价的升高,抗体对鸡的攻毒保护率也随之升高,即抗体效价与攻毒保护率呈正相关。
Three batches of Ⅰ type 4 avian adenovirus antibodies produced in laboratory were diluted by Phosphate butter solution respectively for the AGP titer of 18, 14 and 12. Antibodies with different titer were injected to21 and 42 day old SPF chickens. After 24 hours, SPF chickens were attacked with group I type 4 avian adenovirus.The results showed that as the titer of antibodies increased, the virus attack protection also increased. The antibody titer was positively correlated with the protection rate of attack.
Three batches of Ⅰ type 4 avian adenovirus antibodies produced in laboratory were diluted by Phosphate butter solution respectively for the AGP titer of 18, 14 and 12. Antibodies with different titer were injected to21 and 42 day old SPF chickens. After 24 hours, SPF chickens were attacked with group I type 4 avian adenovirus.The results showed that as the titer of antibodies increased, the virus attack protection also increased. The antibody titer was positively correlated with the protection rate of attack.
引文
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[2]初欢欢,单虎,郑秀云,等.9株Ⅰ群禽腺病毒的分离鉴定及hexon基因的遗传变异分析[J].中国兽医杂志,2017(1):7-9.
[3]王亚楠,韩涛,李纯玲,等.禽腺病毒Ⅰ群的研究现状[J].中国动物保健,2017,19(1):61-64.
[4]藏玉婷,张杨,王彬,等.小鹅瘟卵黄抗体效价与攻毒保护相关性试验[J].广东畜牧兽医科技,2015,40(3):39-40.
[5]刘吉山,肖跃强,于雪,等.麻鸭感染Ⅰ群禽腺病毒4型的致病及防治研究[J].中国兽医科学,2017(9):1 112-1 117.
[6]孙德君,柴华,冯艳男,等.禽Ⅰ群腺病毒血清4型感染的诊断与防治[J].兽医导刊,2017(15):29-30.
[7]张玉霞,袁小远,徐怀英,等.鸡腺病毒Ⅰ群血清4型的分离鉴定与防治[J].家禽科学,2016(10):29-30.
[8]王慧,程小果,郭建友,等.近期鸡群I群禽腺病毒的流行与防治[J].上海畜牧兽医通讯,2016(2):62-63.
[9]李敏,马玉峰,杨振.Ⅰ型禽腺病毒在我国鸡群中的流行情况和防控措施[J].兽医导刊,2017(1):30-32.