铝胁迫对3种油茶初生根生理特性的影响
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摘要
为耐铝基因筛选和油茶耐铝品种的选育提供参考,以广宁红花油茶、高州油茶和普通油茶为材料,采用水培法,研究在不同浓度、不同时间铝胁迫条件下初生根的生理变化。结果表明:铝胁迫24h后,3种油茶初生根生长受到抑制,广宁红花油茶、高州油茶和普通油茶在铝浓度为4mmol/L时的初生根相对伸长率分别为71.94%、54.57%和47.25%,初生根活力分别为89.05mgTTF/(g·h)、120.06mgTTF/(g·h)和87.05mgTTF/(g·h)。3种油茶初生根质膜透性随铝浓度升高和处理时间延长增大,胁迫结束时相对电导率达到峰值,广宁红花油茶、高州油茶和普通油茶在铝浓度为4mmol/L时,其相对电导率分别为65.80%、73.26%和75.28%。处理周期内,3种油茶SOD活性呈下降趋势,POD、CAT活性先升高后降低。胁迫结束时广宁红花油茶、高州油茶和普通油茶在铝浓度为4 mmol/L时的初生根MDA含量分别为11.72nmol/g、35.56nmol/g和21.46nmol/g,游离脯氨酸含量分别为610.97μg/g、649.63μg/g和687.75μg/g。油茶可通过一系列生理变化耐受铝胁迫伤害,不同油茶耐铝性存在差异,通过对铝胁迫条件下油茶初生根的各项生理指标比较分析得出,3中油茶中广宁红花油茶耐铝性最好,高州油茶次之,普通油茶最差。
The physiological change of primary roots of Camellia semiserrata,Camellia gauchowensis and Camellia oleifera was analyzed by water culture method under Al stress with different concentration and treatment days to provide a reference for gene screening of Al tolerance and breeding of oil tea species with Al tolerance.Results:The primary root growth of three oil tea species is inhibited after 1d Al stress.The relative elongation and activity of primary roots of C.semiserrata,C.gauchowensis and C.oleiferaare 71.94% and 89.05mgTTF/(g·h),54.57% and 120.06mgTTF/(g·h),47.25% and87.05mgTTF/(g·h)respectively when Al concentration is 4mmol/L.The membrane permeability of primary roots of three oil tea species increases with increase of Al concentration and treatment hours and their relative conductance reaches the peak value when Al stress ends.The relative conductance of C.semiserrata,C.gauchowensis and C.oleiferais 65.80%,73.26% and 75.28% respectively when Al concentration is 4mmol/L.The SOD activity of three oil tea species shows a declining trend but their POD and CAT activity presents a first rising and then declining trend under Al stress.The MDA and free proline content in primary roots of C.semiserrata,C.gauchowensis and C.oleiferais 11.72nmol/g and610.97μg/g,35.56nmol/g and 649.63μg/g,21.46nmol/g and 687.75μg/g respectively when Al concentration is 4mmol/L.The oil tea can resist Al stress by a series of physiological change but there is a difference in Al tolerance among different oil tea species.The Al tolerance of C.semiserratais the best,followed by C.gauchowensis and C.oleifera.
The physiological change of primary roots of Camellia semiserrata,Camellia gauchowensis and Camellia oleifera was analyzed by water culture method under Al stress with different concentration and treatment days to provide a reference for gene screening of Al tolerance and breeding of oil tea species with Al tolerance.Results:The primary root growth of three oil tea species is inhibited after 1d Al stress.The relative elongation and activity of primary roots of C.semiserrata,C.gauchowensis and C.oleiferaare 71.94% and 89.05mgTTF/(g·h),54.57% and 120.06mgTTF/(g·h),47.25% and87.05mgTTF/(g·h)respectively when Al concentration is 4mmol/L.The membrane permeability of primary roots of three oil tea species increases with increase of Al concentration and treatment hours and their relative conductance reaches the peak value when Al stress ends.The relative conductance of C.semiserrata,C.gauchowensis and C.oleiferais 65.80%,73.26% and 75.28% respectively when Al concentration is 4mmol/L.The SOD activity of three oil tea species shows a declining trend but their POD and CAT activity presents a first rising and then declining trend under Al stress.The MDA and free proline content in primary roots of C.semiserrata,C.gauchowensis and C.oleiferais 11.72nmol/g and610.97μg/g,35.56nmol/g and 649.63μg/g,21.46nmol/g and 687.75μg/g respectively when Al concentration is 4mmol/L.The oil tea can resist Al stress by a series of physiological change but there is a difference in Al tolerance among different oil tea species.The Al tolerance of C.semiserratais the best,followed by C.gauchowensis and C.oleifera.
引文
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[11]陈荣府,董晓英,赵学强,等.木本植物适应酸性土壤机理的研究进展——以胡枝子(Lespedeza bicolor)和油茶(Camellia oleifera)为例[J].土壤,2015,47(2):252-258.
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[14]陈爱葵,韩瑞宏,李东洋,等.植物叶片相对电导率测定方法比较研究[J].广东教育学院学报,2010,30(5):88-91.
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[17]周楠,韦冬萍,刘鹏,等.铝毒胁迫对两种基因型油菜苗期根系形态及生理特性的影响[J].中国油料作物学报,2008,30(4):443-449.
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[19]周媛,章艺,吴玉环,等.酸铝胁迫对栝楼根系生长及铝积累的影响[J].农业环境科学学报,2011,30(12):2434-2439.
[20]郭天荣,陈丽萍,冯其芳,等.铝、镉胁迫对空心菜生长及抗氧化特性的影响[J].核农学报,2015,29(3):571-576.
[21]TAMáS L,DUDíKOVáJ,UR EKOVK,et al.Effect of cadmium and temperature on the lipoxygenase activity in barley root tip[J].Protoplasma,2009,235:17-25.
[22]黄筱敏,刘承军,WEEMBA L K,等.柱花草品种间耐酸铝胁迫差异及其机理初探[J].广西农业科学,2007,38(1):38-42.
[23]王伟,杨野,郭再华.铝胁迫对不同耐铝小麦品种根生理结构及活性氧代谢酶的影响[J].华中农业大学学报,2010,29(6):715-720.
[24]李俊钰,胥晓,杨鹏,等.铝胁迫对青杨雌雄幼苗生理生态特征的影响[J].应用生态学报,2012,23(1):45-50.
[25]杨野,郭再华,耿明建,等.铝胁迫下不同耐铝小麦品种活性氧代谢差异及与小麦耐铝性的关系[J].生态环境学报,2010,19(1):177-182.
[26]刘鹏,徐根娣,姜雪梅,等.铝对大豆幼苗膜脂过氧化和体内保护系统的影响[J].农业环境科学学报,2004,23(1):51-54.
[27]蔡琪敏,陈洁,张志祥,等.铜胁迫对两种苔藓植物生理生化的影响[J].浙江林业科技,2008,28(6):24-27.
[2]武孔焕,陈奇,李昆志,等.铝胁迫对黑大豆膜脂过氧化及抗氧化酶活性的影响[J].西北植物学报,2012,32(3):511-517.
[3]KOCHIAN L V,HOEKENGA O A,PINEROS M A.How do crop plants tolerate acid soil?Mechanisms of aluminum tolerance and phosphorus efficiency[J].Annual Review of Plant Biology,2004,55:459-493.
[4]YAMAMOTO Y,KOBAYASHI Y,DEVI S R,et al.Oxidative stress triggered by aluminum in plant roots[J].Plant and Soil,2003,255(1):239-243.
[5]PAN J W,ZHENG K,YE D,et al.Aluminum-induced ultraweak luminescence changes and sister-chromatid exchanges in root tip cells of barley[J].Plant Science,2004,167(6):1391-1399.
[6]陈泰林,钱春梅,张建军,等.植物铝胁迫响应机制的研究进展[J].热带农业科学,2010,30(2):37-48.
[7]贺根和,刘强,彭水娥.铝胁迫对野生油茶光合特性的影响[J].湖北农业科学,2010,49(7):1593-1595,1598.
[8]谭晓风,袁军,李泽,等.铝磷对普通油茶根系形态和活力及物质分配的影响[J].中南林业科技大学学报,2011,31(12):108-110.
[9]HE G H,ZHANG J F,HU X H,et al.Effect of aluminum toxicity and phosphorus deficiency on the growth and photosynthesis of oil tea(Camellia oleifera Abel.)seedlings in acidic red soils[J].Acta Physiologiae Plantarum,2011,33(4):1285-1292.
[10]OSAWA H,IKEDA S,TANGE T.The rapid accumulation of aluminum is ubiquitous in both the evergreen and deciduous leaves of Theaceae and Ternstroemiaceae plants over a wide pH range in acidic soils[J].Plant and Soil,2013,363(1):49-59.
[11]陈荣府,董晓英,赵学强,等.木本植物适应酸性土壤机理的研究进展——以胡枝子(Lespedeza bicolor)和油茶(Camellia oleifera)为例[J].土壤,2015,47(2):252-258.
[12]张立军,樊金娟.植物生理学实验教程[M].北京:中国农业大学出版社,2007.
[13]刘萍,李明军.植物生理学实验技术[M].北京:科学出版社,2007:42-44.
[14]陈爱葵,韩瑞宏,李东洋,等.植物叶片相对电导率测定方法比较研究[J].广东教育学院学报,2010,30(5):88-91.
[15]ZHAO Z Q,MA J F,SATO K,et al.Differential Al resistance and citrate secretion in barley(Hordeum vulgare L.)[J].Planta,2003,217(5):794-800.
[16]孟赐福,姜培坤,曹志洪,等.植物的铝毒机制和耐铝机制研究进展[J].浙江农业学报,2009,21(4):411-416.
[17]周楠,韦冬萍,刘鹏,等.铝毒胁迫对两种基因型油菜苗期根系形态及生理特性的影响[J].中国油料作物学报,2008,30(4):443-449.
[18]王小平.茶树(Camellia sinensis L.)幼苗品质及生理对铝氟交互处理响应的研究[D].金华:浙江师范大学,2006.
[19]周媛,章艺,吴玉环,等.酸铝胁迫对栝楼根系生长及铝积累的影响[J].农业环境科学学报,2011,30(12):2434-2439.
[20]郭天荣,陈丽萍,冯其芳,等.铝、镉胁迫对空心菜生长及抗氧化特性的影响[J].核农学报,2015,29(3):571-576.
[21]TAMáS L,DUDíKOVáJ,UR EKOVK,et al.Effect of cadmium and temperature on the lipoxygenase activity in barley root tip[J].Protoplasma,2009,235:17-25.
[22]黄筱敏,刘承军,WEEMBA L K,等.柱花草品种间耐酸铝胁迫差异及其机理初探[J].广西农业科学,2007,38(1):38-42.
[23]王伟,杨野,郭再华.铝胁迫对不同耐铝小麦品种根生理结构及活性氧代谢酶的影响[J].华中农业大学学报,2010,29(6):715-720.
[24]李俊钰,胥晓,杨鹏,等.铝胁迫对青杨雌雄幼苗生理生态特征的影响[J].应用生态学报,2012,23(1):45-50.
[25]杨野,郭再华,耿明建,等.铝胁迫下不同耐铝小麦品种活性氧代谢差异及与小麦耐铝性的关系[J].生态环境学报,2010,19(1):177-182.
[26]刘鹏,徐根娣,姜雪梅,等.铝对大豆幼苗膜脂过氧化和体内保护系统的影响[J].农业环境科学学报,2004,23(1):51-54.
[27]蔡琪敏,陈洁,张志祥,等.铜胁迫对两种苔藓植物生理生化的影响[J].浙江林业科技,2008,28(6):24-27.