脱落酸对水稻根系生长素合成与运输的调控
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摘要
利用一系列不同浓度的脱落酸(ABA)处理水稻幼苗根系,观察水稻初生根根长、冠根数目、侧根数目及长度、DR5::GUS转基因材料GUS活性、生长素的合成及运输相关基因表达水平等变化.结果表明,ABA可以抑制水稻初生根的伸长、冠根和侧根的发生和伸长,并且抑制效应呈现剂量效应.DR5::GUS活性在整个根系中随ABA处理浓度升高而减弱,但根尖部位的DR5::GUS活性经过ABA处理后表现增强;生长素合成相关基因YUCCA2、YUCCA4、YUCCA7,生长素运输载体基因AUX3、AUX5、PiN1b、PiN2、PiN5a、PiN9、PiN10a均显著下降表达.
The rice seedlings were treated by difference concentration of ABA( abscisic acid). The root phenotype,DR5: : GUS expression pattern,the changes of auxin biosynthesis and transport genes expression in were studied.The results demonstrated that primary root elongation,crown root initiation,and lateral root initiation and elongation was inhibited in ABA treated seedling. Besides,GUS activity was also reduced in the ABA treated DR5: : GUS transgenic plants. On the contrary,GUS activity in root tip increased with ABA treatment. Furthermore,Both auxin biosynthesis genes YUCCA2,YUCCA4,YUCCA7,and auxin transport genes AUX3,AUX5,PIN1 b,PIN2,PIN5 a,PIN9,PIN10 a were down-regulated in ABA treated rice root.
The rice seedlings were treated by difference concentration of ABA( abscisic acid). The root phenotype,DR5: : GUS expression pattern,the changes of auxin biosynthesis and transport genes expression in were studied.The results demonstrated that primary root elongation,crown root initiation,and lateral root initiation and elongation was inhibited in ABA treated seedling. Besides,GUS activity was also reduced in the ABA treated DR5: : GUS transgenic plants. On the contrary,GUS activity in root tip increased with ABA treatment. Furthermore,Both auxin biosynthesis genes YUCCA2,YUCCA4,YUCCA7,and auxin transport genes AUX3,AUX5,PIN1 b,PIN2,PIN5 a,PIN9,PIN10 a were down-regulated in ABA treated rice root.
引文
[1] DE SMET I,ZHANG H,INZE D,et al. A novel role for abscisic acid emerges from underground[J]. Trends in Plant Sci-ence,2006,11(9):434-439.
[2] DING Z,DE SMET I. Localised ABA signalling mediates root growth plasticity[J]. Trends in Plant Science,2013,18(10):533-535.
[3] CUTLER S R,RODRIGUEZ P L,FINKELSTEIN R R,et al. Abscisic acid:emergence of a core signaling network[J]. An-nual Review of Plant Biology,2010,61(1):651-679.
[4]李金涛,潘娟娟,闫寒,等.植物激素调控水稻冠根发育研究进展[J].信阳师范学院学报(自然科学版),2018,31(3):511-516.LI Jintao,PAN Juanjuan,YAN Han,et al. Research advances of plant hormone regulating crown root development in rice[J]. Journal of Xinyang Normal University(Natural Science Edition),2018,31(3):511-516.
[5] DEL BIANCO M,KEPINSKI S. Context,specicity,and self-organization in auxin response[J]. Cold Spring Harbor Per-spective in Biology,2011,3(1):a001578.
[6] MASHIGUCHUI K,TANAKA K,SAKAI T,et al. The main auxin biosynthesis pathway in Arabidopsis[J]. Proceedings ofthe National Academy of Sciences of the United States of America,2011,108(45):18512-18517.
[7] JUNG J K,MCCOUCH S. Getting to the roots of it:genetic and hormonal control of root architecture[J]. Frontier in PlantScience,2013,4,doi:10.3389/fpls.2013.00186.
[8] ZHAO H M,MA T F,WANG X,et al. Os AUX1 controls lateral root initiation in rice(Oryza sativa L.)[J]. Plant Cell andEnvironment,2015,38(11):2208-2222.
[9] LEYSER O. Auxin distribution and plant pattern formation:How many angels can dance on the point of PIN?[J] Cell,2005,121(6):819-822.
[10] XU M,ZHU L,SHOU H,et al. PIN1 family gene,Os PIN1,involved in auxin-dependent adventitious root emergence andtillering in rice[J]. Plant Cell Physiology,2005,46(10):1674-1681.
[11] WANG L,GUO M,LI Y,et al. LARGE ROOT ANGLE1,encoding Os PIN2,is involved in root system architecture in rice[J]. Journal of Experimental Botany,2017,69(3):385-397.
[12] VANSTRAELEN M,BENKOVA E. Hormonal interactions in the regulation of plant development[J]. Annual Review of Celland Development Biology,2012,28(28):463-487.
[13] XU W F,JIA L G,SHI W M.,et al. Abscisic acid accumulation modulates auxin transport in the root tip to enhance protonsecretion for maintaining root growth under moderate water stress[J].New Phytologist,2013,197(1):139-150.
[14] BRADY S M,SARKAR S F,BONETTA D,et al. The ABSCISIC ACID INSENSITIVE 3(ABI3)gene is modulated by far-nesylation and is involved in auxin signalling and lateral root development in Arabidopsis[J]. Plant Journal,2003,34(1):67-75.
[15] SHKOLNIK-INBAR D,BAR-ZVI D.ABI4 mediates abscisic acid and cytokinin inhibition of lateral root formation by reduc-ing polar auxin transport in Arabidopsis[J]. Plant Cell,2010,22(11):3560-3573.
[16] JEON E,KANG N Y,CHO C,et al. LBD14/ASL17 positively regulates lateral root formation and is involved in ABA re-sponse for root architecture in arabidopsis[J]. Plant&Cell Physiology,2017,58(12):2190-2201.
[17] WANG T,LI C,WU Z,et al. Abscisic acid regulates auxin homeostasis in rice root tips to promote root hair elongation[J].Frontier in Plant Science,2017,8:1121.
[18] GONG X,FLORES-VERGARA M A,HONG J H,et al. SEUSS integrates gibberellin signaling with transcriptional inputsfrom the SHR-SCR-SCL3 module to regulate middle cortex formation in the arabidopsis root[J]. Plant Physiology,2016,170(3):1675-1683.
[2] DING Z,DE SMET I. Localised ABA signalling mediates root growth plasticity[J]. Trends in Plant Science,2013,18(10):533-535.
[3] CUTLER S R,RODRIGUEZ P L,FINKELSTEIN R R,et al. Abscisic acid:emergence of a core signaling network[J]. An-nual Review of Plant Biology,2010,61(1):651-679.
[4]李金涛,潘娟娟,闫寒,等.植物激素调控水稻冠根发育研究进展[J].信阳师范学院学报(自然科学版),2018,31(3):511-516.LI Jintao,PAN Juanjuan,YAN Han,et al. Research advances of plant hormone regulating crown root development in rice[J]. Journal of Xinyang Normal University(Natural Science Edition),2018,31(3):511-516.
[5] DEL BIANCO M,KEPINSKI S. Context,specicity,and self-organization in auxin response[J]. Cold Spring Harbor Per-spective in Biology,2011,3(1):a001578.
[6] MASHIGUCHUI K,TANAKA K,SAKAI T,et al. The main auxin biosynthesis pathway in Arabidopsis[J]. Proceedings ofthe National Academy of Sciences of the United States of America,2011,108(45):18512-18517.
[7] JUNG J K,MCCOUCH S. Getting to the roots of it:genetic and hormonal control of root architecture[J]. Frontier in PlantScience,2013,4,doi:10.3389/fpls.2013.00186.
[8] ZHAO H M,MA T F,WANG X,et al. Os AUX1 controls lateral root initiation in rice(Oryza sativa L.)[J]. Plant Cell andEnvironment,2015,38(11):2208-2222.
[9] LEYSER O. Auxin distribution and plant pattern formation:How many angels can dance on the point of PIN?[J] Cell,2005,121(6):819-822.
[10] XU M,ZHU L,SHOU H,et al. PIN1 family gene,Os PIN1,involved in auxin-dependent adventitious root emergence andtillering in rice[J]. Plant Cell Physiology,2005,46(10):1674-1681.
[11] WANG L,GUO M,LI Y,et al. LARGE ROOT ANGLE1,encoding Os PIN2,is involved in root system architecture in rice[J]. Journal of Experimental Botany,2017,69(3):385-397.
[12] VANSTRAELEN M,BENKOVA E. Hormonal interactions in the regulation of plant development[J]. Annual Review of Celland Development Biology,2012,28(28):463-487.
[13] XU W F,JIA L G,SHI W M.,et al. Abscisic acid accumulation modulates auxin transport in the root tip to enhance protonsecretion for maintaining root growth under moderate water stress[J].New Phytologist,2013,197(1):139-150.
[14] BRADY S M,SARKAR S F,BONETTA D,et al. The ABSCISIC ACID INSENSITIVE 3(ABI3)gene is modulated by far-nesylation and is involved in auxin signalling and lateral root development in Arabidopsis[J]. Plant Journal,2003,34(1):67-75.
[15] SHKOLNIK-INBAR D,BAR-ZVI D.ABI4 mediates abscisic acid and cytokinin inhibition of lateral root formation by reduc-ing polar auxin transport in Arabidopsis[J]. Plant Cell,2010,22(11):3560-3573.
[16] JEON E,KANG N Y,CHO C,et al. LBD14/ASL17 positively regulates lateral root formation and is involved in ABA re-sponse for root architecture in arabidopsis[J]. Plant&Cell Physiology,2017,58(12):2190-2201.
[17] WANG T,LI C,WU Z,et al. Abscisic acid regulates auxin homeostasis in rice root tips to promote root hair elongation[J].Frontier in Plant Science,2017,8:1121.
[18] GONG X,FLORES-VERGARA M A,HONG J H,et al. SEUSS integrates gibberellin signaling with transcriptional inputsfrom the SHR-SCR-SCL3 module to regulate middle cortex formation in the arabidopsis root[J]. Plant Physiology,2016,170(3):1675-1683.