内源H_2O_2对水稻种子萌发的影响
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摘要
通过用不同浓度的烟酰胺腺嘌呤二核苷酸磷酸(nicotinamide adenine dinucleotide phosphate, NADPH)合成酶抑制剂碘二苯(diphenylene iodonium, DPI)及过氧化氢(hydrogen peroxide, H_2O_2)清除剂二甲基硫脲(dimethylthiourea,DMTU)分别培养水稻(Oryza sativa L.)种子,研究内源H_2O_2对种子萌发过程胚根、胚芽和胚根根尖活力等的影响。结果表明,DPI及DMTU培养的水稻种子其胚根生长和胚芽生长均受到抑制,尤其是DPI对胚根生长的抑制作用更为显著。DPI和DMTU对水稻种子萌发的影响均呈现出浓度效应,即浓度越高,抑制作用越强。其中,DPI对水稻种子萌发的抑制作用比DMTU的更为明显。此外,胚根根尖的超氧阴离子(superoxideanion,O_2?)和H_2O_2含量随DMTU浓度增大而减少,根尖细胞受损也越严重。由此推测,内源H_2O_2可能参与调控水稻种子萌发过程。
To explore the effect of endogenous hydrogen peroxide(H_2 O_2) on the growth of radicle and plumule, and the apical vitality in the radicle during germination, rice(Oryza sativa L.) seeds were cultured with different concentrations of nicotinamide adenine dinucleotide phosphate(NADPH) synthase inhibitor diphenylene iodonium(DPI) or H_2 O_2 scavenger dimethylthiourea(DMTU). The results showed that the growth of radicle and plumule in the rice seeds cultured with both DMTU and DPI was inhibited, especially that with DPI. Higher concentration of DPI or DMTU resulted in stronger inhibitory effect on rice seed germination. The inhibitory effect of DPI on rice seed germination was more obvious than that of DMTU. In addition, the contents of superoxide anion(O_2-) and H_2 O_2 decreased in the apical of radicle with the increase of DMTU concentration, and the number of damage cell increased. Therefore, It suggested that endogenous H_2 O_2 may be involved in regulating the germination of rice seeds.
To explore the effect of endogenous hydrogen peroxide(H_2 O_2) on the growth of radicle and plumule, and the apical vitality in the radicle during germination, rice(Oryza sativa L.) seeds were cultured with different concentrations of nicotinamide adenine dinucleotide phosphate(NADPH) synthase inhibitor diphenylene iodonium(DPI) or H_2 O_2 scavenger dimethylthiourea(DMTU). The results showed that the growth of radicle and plumule in the rice seeds cultured with both DMTU and DPI was inhibited, especially that with DPI. Higher concentration of DPI or DMTU resulted in stronger inhibitory effect on rice seed germination. The inhibitory effect of DPI on rice seed germination was more obvious than that of DMTU. In addition, the contents of superoxide anion(O_2-) and H_2 O_2 decreased in the apical of radicle with the increase of DMTU concentration, and the number of damage cell increased. Therefore, It suggested that endogenous H_2 O_2 may be involved in regulating the germination of rice seeds.
引文
[1]王琼.水稻种子萌发过程中RAmy1A基因的特异转录因子的筛选与鉴定[D].北京:中国科学院大学,2016.
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[2]Larkindale J,Huang B.Thermotolerance and antioxidant systems in Agrostis stolonifera:involvement of salicylic acid,abscisic acid,calcium,hydrogen peroxide,and ethylene[J].Journal of Plant Physiology,2004,161(4):405-413.
[3]Neill S J,Desikan R,Clarke A,et al.Hydrogen peroxide and nitric oxide as signalling molecules in plants[J].Journal of Experimental Botany,2002,53(372):1237-1247.
[4]Hung S H.Hydrogen peroxide functions as a stress signal in plants[J].Botanical Bulletin of Academia Sinica,2005,46(1):1-10.
[5]徐芳杰.过氧化氢作为信号分子在小麦耐铝性中的作用及机理[D].杭州:浙江大学,2011.
[6]Wang G,Xiao Y,Deng X,et al.Exogenous hydrogen peroxide contributes to heme oxygenase-1 delaying programmed cell death in isolated aleurone layers of rice subjected to drought stress in a c GMP-dependent manner[J].Frontiers in Plant Science,2018,9:84.
[7]孔祥强,罗振,张艳军,等.过氧化氢预处理促进盐胁迫下棉花种子萌发的效应[C]//全国棉花青年学术研讨会,2015.
[8]张凤芝,胡文婷,苏国兴.多胺降解产生H2O2在莴苣种子萌发过程中的作用[J].苏州大学学报(自然科学版),2011,27(1):84-89.
[9]王光辉.HO-1协同H2O2调控干旱诱导的水稻糊粉层PCD的发生[D].海口:海南大学,2014.
[10]张倩倩,蔚潇,贺学勤.过氧化氢清除剂对H2O2处理下中国石竹幼苗不同器官抗氧化系统的影响[J].北方园艺,2016(22):82-86.
[11]蒋景龙.外源过氧化氢抑制山黧豆种子萌发期初生根的向地性生长并诱导其发生水平弯曲[D].兰州:兰州大学,2012.
[12]Lee S E,Shin H T,Hwang H J,et al.Antioxidant activity of extracts from Alpinia katsumadai seed[J].Phytotherapy Research,2003,17(9):1041-1047.
[13]Biondi S.Ectopic expression of maize polyamine oxidase and pea copper amine oxidase in the cell wall of tobacco plants[J].Plant Physiology,2004,134(4):1414.
[14]韩明明.镉锌互作下H2O2对水稻根系生长发育的影响[D].淄博:山东理工大学,2011.
[15]李师翁.H2O2在绿豆不定根形成与发育中的信号作用及其机理[D].兰州:兰州大学,2007.
[16]林莹.老化大豆胚轴细胞死亡生物学基础的研究[D].福州:福建农林大学,2012.