铁皮石斛化学成分的分离与鉴定
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摘要
目的研究铁皮石斛(Dendrobium officinale Kimura et Migo.)中的化学成分。方法采用大孔吸附树脂、正相硅胶、ODS、Sephadex LH-20等柱色谱法和制备型高效液相色谱法等分离技术,对铁皮石斛乙醇提取物化学成分进行分离、纯化,并通过化合物的理化性质与波谱特征鉴定其化合物结构。结果从乙醇提取物中分离并鉴定了9个化合物,分别为反式-3,4,5-三甲氧基肉桂醇(trans-3,4,5-trimethoxyl-cinnamyl alcohol,1)、广玉兰赖宁苷(magnolenin C,2)、officinalioside(3)、moellenoside A (4)、松脂素-4-O-β-D-吡喃葡萄糖(pinoresinol-4-O-β-D-glucopyranoside,5)、erianthridin(6)、ephemeranthol A(7)、2,7-dihydroxy-3,4-dimethoxyphenanthrene(8)和毛兰菲(confusarin,9)。结论化合物1～5为首次从石斛属植物中分离得到。
Objective To study the chemical constituents from Dendrobium officinale Kimura et Migo.Methods The compounds were isolated from ethanol extract of it by chromatographic methods such as macroporous resin,silica gel,ODS,Sephadex LH-20 column chromatography,HPLC and so on. The compounds were identified by the physicochemical property and spectral features. Results Nine compounds were isolated from ethanol extract and their structures were identified as trans-3,4,5-trimethoxyl-cinnamyl alcohol( 1),magnolenin C( 2),officinalioside( 3),moellenoside A( 4),pinoresinol-4-O-β-Dglucopyranoside( 5),erianthridin( 6),ephemeranthol A( 7),2,7-dihydroxy-3,4-dimethoxyphenanthrene( 8),and confusarin( 9),respectively. Conclusions Compounds 1-5 are obtained from the genus of Dendrobium for the first time.
Objective To study the chemical constituents from Dendrobium officinale Kimura et Migo.Methods The compounds were isolated from ethanol extract of it by chromatographic methods such as macroporous resin,silica gel,ODS,Sephadex LH-20 column chromatography,HPLC and so on. The compounds were identified by the physicochemical property and spectral features. Results Nine compounds were isolated from ethanol extract and their structures were identified as trans-3,4,5-trimethoxyl-cinnamyl alcohol( 1),magnolenin C( 2),officinalioside( 3),moellenoside A( 4),pinoresinol-4-O-β-Dglucopyranoside( 5),erianthridin( 6),ephemeranthol A( 7),2,7-dihydroxy-3,4-dimethoxyphenanthrene( 8),and confusarin( 9),respectively. Conclusions Compounds 1-5 are obtained from the genus of Dendrobium for the first time.
引文
[1]叶飞飞,胡雪华,李晓红,等.铁皮石斛研究进展[J].农技服务,2016,33(4):18-19.
[2]李娟,李顺祥,黄丹,等.铁皮石斛资源、化学成分及药理作用研究进展[J].科技导报,2011,29(18):74-79.
[3]郭丽宏.铁皮石斛资源、化学成分及药理作用研究进展[J].亚太传统医药,2014,10(21):45-46.
[4] YANG Y,JIANG J Z,LUOBU Q,et al. The fungicidal terpenoids and essential oil from Litsea cubeba in Tibet[J]. M olecules,2010,15(10):7075.
[5]冯锋,朱明晓,谢宁.毛冬青化学成分研究[J].中国药学杂志,2008,43(10):732-736.
[6]杨炳友,杨春丽,刘艳,等.洋金花根中苯丙素类化学成分研究[J].中草药,2017,48(14):2820-2826.
[7] ZHENG X K,LI K K,WANG Y Z,et al. A new dihydrobenzofuran lignanoside from Selaginella moellendorffii Hieron[J]. Chinese Chemical Letters,2008,19(1):79-81.
[8]任慧,董丽梅,周忠玉,等.南美蟛蜞菊化学成分研究[J].中药材,2015,38(7):1426-1429.
[9] YANG H,GONG Y,WANG Z,et al. Studies on chemical constituents of Dendrobium chrysotoxum[J]. Chinese Traditional&Herbal Drugs,2001,31(4):304-306.
[10]KIM J H,OH S Y,HAN S B,et al. Anti-inflammatory effects of Dendrobium nobile,derived phenanthrenes in LPS-stimulated murine macrophages[J]. Archives of Pharmacal Research,2015,38(6):1117-1126.
[11]HUANG Y L,LAY H L,SHEN C C,et al. Stilbenoids from the stems of Dendrobium sonia[J]. Chinese Pharmaceutical Journal,2000,52(6):305-311.
[2]李娟,李顺祥,黄丹,等.铁皮石斛资源、化学成分及药理作用研究进展[J].科技导报,2011,29(18):74-79.
[3]郭丽宏.铁皮石斛资源、化学成分及药理作用研究进展[J].亚太传统医药,2014,10(21):45-46.
[4] YANG Y,JIANG J Z,LUOBU Q,et al. The fungicidal terpenoids and essential oil from Litsea cubeba in Tibet[J]. M olecules,2010,15(10):7075.
[5]冯锋,朱明晓,谢宁.毛冬青化学成分研究[J].中国药学杂志,2008,43(10):732-736.
[6]杨炳友,杨春丽,刘艳,等.洋金花根中苯丙素类化学成分研究[J].中草药,2017,48(14):2820-2826.
[7] ZHENG X K,LI K K,WANG Y Z,et al. A new dihydrobenzofuran lignanoside from Selaginella moellendorffii Hieron[J]. Chinese Chemical Letters,2008,19(1):79-81.
[8]任慧,董丽梅,周忠玉,等.南美蟛蜞菊化学成分研究[J].中药材,2015,38(7):1426-1429.
[9] YANG H,GONG Y,WANG Z,et al. Studies on chemical constituents of Dendrobium chrysotoxum[J]. Chinese Traditional&Herbal Drugs,2001,31(4):304-306.
[10]KIM J H,OH S Y,HAN S B,et al. Anti-inflammatory effects of Dendrobium nobile,derived phenanthrenes in LPS-stimulated murine macrophages[J]. Archives of Pharmacal Research,2015,38(6):1117-1126.
[11]HUANG Y L,LAY H L,SHEN C C,et al. Stilbenoids from the stems of Dendrobium sonia[J]. Chinese Pharmaceutical Journal,2000,52(6):305-311.