nm23蛋白在前列腺癌患者中的表达与结核菌L型感染的相关性研究
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摘要
目的探讨转移抑制基因nm23及结核菌L型感染与前列腺癌发病的相关性。方法选取2007年1月-2017年1月医院收治的前列腺癌患者580例作为研究对象。通过免疫组织化学检验,观察nm23蛋白及nm23mRNA在不同分期前列腺癌患者中的表达情况,记录所有前列腺癌患者中结核菌L型感染概率,分析结核菌L型感染与前列腺癌的关系。结果 580例前列腺癌患者中,nm23蛋白表达阳性患者共297例(51.21%),nm23mRNA阳性检出301例(51.90%);随病理分级和临床分期增高,nm23蛋白、mRNA的阳性检出率逐渐降低,结核菌L型感染检出率逐渐增高(P<0.05);580例患者中,有213例检出前列腺癌转移,非转移患者nm23蛋白、nm23mRNA的阳性检出率高于转移患者(P<0.05);非转移组结核菌L型感染检出率低于转移组(P<0.05)。结论 nm23蛋白在前列腺肿瘤中表达情况受病理分级和临床分期影响,提示nm23蛋白可作为评估前列腺癌患者肿瘤分化程度和预后的重要指标。随前列腺癌病情进展,结核菌L型感染发生风险增加,提示结核菌L型感染可能影响前列腺癌的发生和进展,值得临床进一步研究。
OBJECTIVE To explore the metastasis suppressor gene nm23 and the correlation between Mycobacterium tuberculosis L-form infection and pathogenesis of prostate cancer.METHODS A total of 580 prostate cancer patients who were treated in hospitals from Jan 2007 to Jan 2017 were recruited as the study objects.The expression of nm23 protein and nm23 mRNA in the patients with different stages of prostate cancer was observed by means of immunohistochemistry test,the probabilities of the M.tuberculosis L-form infection were recorded,and the correlation between the M.tuberculosis L-form infection and the prostate cancer was analyzed.RESULTS Of the 580 prostate cancer patients,297(51.21%)were tested positive for nm23 protein,and 301(51.90%)were tested positive for nm23 mRNA.With the increase of tumor pathological grade and clinical stage,tjhe positive rates of nm23 protein and mRNA were gradually decreased,while the detection rate of M.tuberculosis L-form infection was gradually increased(P<0.05).Among the 580 patients,213 were diagnosed with prostate cancer metastasis;the positive rates of nm23 protein and nm23 mRNA of the patients without metastasis were significantly higher than those of the patients with metastasis(P<0.05);the incidence of Mt.uberculosis L-form infection was lower in the patients without metastasis than in the patients with metastasis(P<0.05).CONCLUSIONThe expression of nm23 protein is affected by the pathological grade and clinical stage,suggesting that nm23 protein can be used as an important index for evaluation of the degree of tumor differentiation and prognosis of the prostate cancer.The risk of M.tuberculosis L-form infection is increased with the progress of prostate cancer,indicating that the M.tuberculosis L-form infection may affect the occurrence and progression of prostate cancer,and it is worthy to be promoted in the hospital.
OBJECTIVE To explore the metastasis suppressor gene nm23 and the correlation between Mycobacterium tuberculosis L-form infection and pathogenesis of prostate cancer.METHODS A total of 580 prostate cancer patients who were treated in hospitals from Jan 2007 to Jan 2017 were recruited as the study objects.The expression of nm23 protein and nm23 mRNA in the patients with different stages of prostate cancer was observed by means of immunohistochemistry test,the probabilities of the M.tuberculosis L-form infection were recorded,and the correlation between the M.tuberculosis L-form infection and the prostate cancer was analyzed.RESULTS Of the 580 prostate cancer patients,297(51.21%)were tested positive for nm23 protein,and 301(51.90%)were tested positive for nm23 mRNA.With the increase of tumor pathological grade and clinical stage,tjhe positive rates of nm23 protein and mRNA were gradually decreased,while the detection rate of M.tuberculosis L-form infection was gradually increased(P<0.05).Among the 580 patients,213 were diagnosed with prostate cancer metastasis;the positive rates of nm23 protein and nm23 mRNA of the patients without metastasis were significantly higher than those of the patients with metastasis(P<0.05);the incidence of Mt.uberculosis L-form infection was lower in the patients without metastasis than in the patients with metastasis(P<0.05).CONCLUSIONThe expression of nm23 protein is affected by the pathological grade and clinical stage,suggesting that nm23 protein can be used as an important index for evaluation of the degree of tumor differentiation and prognosis of the prostate cancer.The risk of M.tuberculosis L-form infection is increased with the progress of prostate cancer,indicating that the M.tuberculosis L-form infection may affect the occurrence and progression of prostate cancer,and it is worthy to be promoted in the hospital.
引文
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[22]刘勇,赵芳芳,夏佩莹.p^16和bcl-2基因与结核分枝杆菌L型感染在前列腺癌中的表达及意义[J].中华全科医学,2005,3(5):379-381.
[23]Zhang L,Li L,Wei H,et al.Transcriptional factor FOXO3negatively regulates the expression of nm23-H1in nonsmall cell lung cancer[J].Thorac Cancer,2016,7(1):9-16.
[24]Udager AM,DeMarzo AM,Shi Y,et al.Concurrent nuclear ERG and MYC protein overexpression defines a subset of locally advanced prostate cancer:potential opportunities for synergistic targeted therapeutics[J].Prostate,2016,76(9):845-853.
[2]段秀枝,陶志华.PCA3基因与前列腺癌的诊断及其应用价值研究[J].中华检验医学杂志,2016,39(8):568-570.
[3]闫存玲,李志艳,何群,等.血清前列腺特异性抗原同源异构体2在前列腺癌诊断中的应用价值[J].中华检验医学杂志,2015,38(12):813-817.
[4]中华医学会泌尿外科分会.前列腺癌诊断治疗指南[J].继续医学教育,2007,21(6):30-39.
[5]Gerke T,Daniela B,Beltran H,et al.Abstract 4740:loss of the tumor suppressor zinc finger protein-36and risk of lethal prostate cancer[J].Cancer Res,2017,77(13):4740-4742.
[6]Ando M,Mukai Y,Ushijima RI,et al.Disseminated Mycobacterium tuberculosis infection masquerading as metastasis after heavy ion radiotherapy for prostate cancer[J].Intern Med,2016,55(22):3387-3392.
[7]《中华结核和呼吸杂志》编辑委员会.结核分支杆菌L型的检测方法(试行)[J].中华结核和呼吸杂志,2003,26(2):67-69.
[8]Zhang K,Chen X,Zhou J,et al.Association between MMP2-1306C/T polymorphism and prostate cancer susceptibility:a meta-analysis based on 3906subjects[J].Oncotarget,2017,8(27):45020-45029.
[9]Lv Y,Guo S,Li XG,et al.Sputum and serum microRNA-144levels in patients with tuberculosis before and after treatment[J].Int J Infect Dis,2016,43(8):68-73.
[10]Jeon J,Kim TJ,Park HS,et al.Malignant pleural effusion from metastatic prostate cancer:a case report with unusual cytologic findings[J].J Pathol Transl Med,2018,52(4):257-261.
[11]Lee SY,Choi SH.Treatment experience for incidentally diagnosed asymptomatic prostate tuberculosis in a patient with history of BCG intravesical therapy[J].Urol Case Rep,2017,17:39-41.
[12]Joneja U,Short WR,Roberts AL.Disseminated tuberculosis with prostatic abscesses in an immunocompromised patient-A case report and review of literature[J].IDCases,2016,5:15-20.
[13]邵鹏飞,殷长军,李杰,等.腹腔镜下前列腺癌根治术治疗高危前列腺癌的临床效果分析[J].中华外科杂志,2015,53(11):847-851.
[14]Yang T,Chen BZ,Li DF,et al.Reduced nm23protein level correlates with worse clinicopathologic features in colorectal cancers:a meta-analysis of pooled data[J].Medicine,2016,95(4):e2589.
[15]Rasool RU,Nayak D,Chakraborty S,et al.Differential regulation of nm23-H1under hypoxic and serum starvation conditions in metastatic cancer cells and its implication in EMT[J].Eur J Cell Biol,2017,96(2):164-171.
[16]刘喜波,苏晓英,傅燕萍,等.p53、nm23在前列腺癌中的表达和意义[J].中国煤炭工业医学杂志,2002,5(4):325-327.
[17]臧晋,严春寅,张炜.nm23/NDPK在前列腺癌中的表达及意义[J].苏州大学学报:医学版,2003,23(5):548-550.
[18]Dzhivhuho GA,Nangammbi TC,Samie A.Association of interleukin-8gene polymorphisms in HIV patients with opportunistic infections in Limpopo Province,South Africa[J].Genet Mol Res,2016,15(1):15-19.
[19]Rastogi A,Ali A,Tan SH,et al.Autoantibodies against oncogenic ERG protein in prostate cancer:potential use in diagnosis and prognosis in a panel with C-MYC,AMACR and HERV-K Gag[J].Genes Cancer,2016,7(11-12):394-413.
[20]Huang M,Narita S,Inoue T,et al.Fatty acid binding protein4enhances prostate cancer progression by upregulating matrix metalloproteinases and stromal cell cytokine production[J].Oncotarget,2017,8(67):111780-111794.
[21]赵芳芳,杨正梅,汪万英.COX-2、Ki-67和结核菌L型感染在前列腺癌中的表达[J].中国微生态学杂志,2010,22(4):346-349.
[22]刘勇,赵芳芳,夏佩莹.p^16和bcl-2基因与结核分枝杆菌L型感染在前列腺癌中的表达及意义[J].中华全科医学,2005,3(5):379-381.
[23]Zhang L,Li L,Wei H,et al.Transcriptional factor FOXO3negatively regulates the expression of nm23-H1in nonsmall cell lung cancer[J].Thorac Cancer,2016,7(1):9-16.
[24]Udager AM,DeMarzo AM,Shi Y,et al.Concurrent nuclear ERG and MYC protein overexpression defines a subset of locally advanced prostate cancer:potential opportunities for synergistic targeted therapeutics[J].Prostate,2016,76(9):845-853.