乙型病毒性肝炎前S1抗原在HBV感染诊断中的价值
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摘要
目的:探讨乙型病毒性肝炎前S1抗原(PreS1-Ag)与乙肝病毒(HBV)感染之间的关系。方法:收集新疆汉族HBV感染200例(其中慢性HBV携带者61例,慢性乙型肝炎75例,肝硬化43例,肝癌21例)和健康对照者200名,运用实时荧光定量PCR检测HBV DNA载量,同时用酶联免疫吸附试验检测前S1抗原及HbeAg等,ALT、AST等生化指标使用罗氏7600自动生化仪检测。结果:各病例组HbeAg阳性、HBV-DNA阳性和PreS1-Ag阳性差异均无统计学意义(P>0.05);ASC组的肝功能指标ALT、AST与其他三组比较均有明显的统计学意义(P<0.05)。HbeAg与PreS1-Ag两种检测指标的阳性率较一致,检测结果具有统计学意义(P<0.05)。结论:PreS1-Ag与HBV-DNA,HBe Ag检测率高度符合,其是一项十分重要的病毒复制指标。本研究提示PreS1-Ag可作为HbeAg和HBV-DNA检测的补充和对照,其与HBV感染存在显著相关性。
Objective: To investigate the relationship between HBV-DNA, pre-S1 antigen and hepatitis B virus(HBV) infection.Methods:200 healthy controls and 200 cases of chronic HBV infection(61 cases of chronic HBV carriers, 75 cases of chronic hepatitis B, cirrhosis of the liver in 43 cases, 21 cases of liver cancer)were collected from Xinjiang Han nationality,Detection of HBV DNA load by real time fluorescence quantitative PCR,meanwhile, enzyme-linked immunosorbent assay was used to detect PreS1 antigen(PreS1-Ag) and HbeAg,We used the Roche 7600 automatic biochemical analyzer ALT, AST and other biochemical indicators.Results:There was no significant difference in HbeAg positive, HBV-DNA positive and preS1 Ag positive differences in each group(P>0.05); The ALT and AST of liver function in ASC group were statistically significant compared with the other three groups(P<0.05).The postive of HbeAg and preS1-Ag was consistent, and the results were statistically significard(P<0.05).Conclusion:PreS1-Ag,HBV-DNA and HBe Ag detection rate was highly consistent and was a very important viral replication index.It was suggested that PreS1-Ag can be used as a supplement and control of HbeAg and HBV-DNA detection.
Objective: To investigate the relationship between HBV-DNA, pre-S1 antigen and hepatitis B virus(HBV) infection.Methods:200 healthy controls and 200 cases of chronic HBV infection(61 cases of chronic HBV carriers, 75 cases of chronic hepatitis B, cirrhosis of the liver in 43 cases, 21 cases of liver cancer)were collected from Xinjiang Han nationality,Detection of HBV DNA load by real time fluorescence quantitative PCR,meanwhile, enzyme-linked immunosorbent assay was used to detect PreS1 antigen(PreS1-Ag) and HbeAg,We used the Roche 7600 automatic biochemical analyzer ALT, AST and other biochemical indicators.Results:There was no significant difference in HbeAg positive, HBV-DNA positive and preS1 Ag positive differences in each group(P>0.05); The ALT and AST of liver function in ASC group were statistically significant compared with the other three groups(P<0.05).The postive of HbeAg and preS1-Ag was consistent, and the results were statistically significard(P<0.05).Conclusion:PreS1-Ag,HBV-DNA and HBe Ag detection rate was highly consistent and was a very important viral replication index.It was suggested that PreS1-Ag can be used as a supplement and control of HbeAg and HBV-DNA detection.
引文
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[6]程振波,谭黎明,李建英,等.乙型肝炎病毒感染者血清HBVLP检测意义及其与Pre S1抗原、HBV DNA之间关系的研究[J].实用预防医学,2016,23(4):487-489
[7]陈华宏,陈谋,洪洁华,等.乙肝前S1抗原在乙肝诊疗中的临床价值以及与HBV-DNA相关性研究[J].实验与检验医学,2017,35(1):102-109
[8]黄之品,杨伟,李增彩,等.216例乙肝患者HBe Ag、HBVDNA、Pre S1-Ag相关性的临床分析[J].辽宁医学院学报,2014,35(3):42-43
[9]陈华宏,陈谋,洪洁华,等.乙肝前S1抗原在乙肝诊疗中的临床价值以及与HBV-DNA相关性研究[J].实验与检验医学,2017,35(1):102-109
[10]Gane EJ.Future anti-HBV strategies[J].Liver Int,2017,37 Suppl1:40-44
[2]罗慧琴,王志刚,李玲,等,乙肝患者血清标志物与前S1抗原及HBV-DNA相关性分析[J].国际检验医学杂志,2013,34(24):3337-3338
[3]Hamid MA,Amin MA,Kassem HH,et al.E-Selection gene polymorphism and coronary artery disease:a genetic association study[J].Heart Mirror Journal,2007,1(2):57-62
[4]皱伟,李功军.乙型肝炎前S1抗原与HBV-DNA检测相关性分析[J].数理医药学杂志,2015,28(2):222-223
[5]于宏建,王亚军,杨树新.HBV标记物、HBV-DNA和pre S1-Ag检测的相关性分析与临床意义[J].吉林医学,2015,36(9):1790
[6]程振波,谭黎明,李建英,等.乙型肝炎病毒感染者血清HBVLP检测意义及其与Pre S1抗原、HBV DNA之间关系的研究[J].实用预防医学,2016,23(4):487-489
[7]陈华宏,陈谋,洪洁华,等.乙肝前S1抗原在乙肝诊疗中的临床价值以及与HBV-DNA相关性研究[J].实验与检验医学,2017,35(1):102-109
[8]黄之品,杨伟,李增彩,等.216例乙肝患者HBe Ag、HBVDNA、Pre S1-Ag相关性的临床分析[J].辽宁医学院学报,2014,35(3):42-43
[9]陈华宏,陈谋,洪洁华,等.乙肝前S1抗原在乙肝诊疗中的临床价值以及与HBV-DNA相关性研究[J].实验与检验医学,2017,35(1):102-109
[10]Gane EJ.Future anti-HBV strategies[J].Liver Int,2017,37 Suppl1:40-44