妊娠期妇女血清乙肝病毒DNA表达载量与前S1抗原表达的相关性分析
摘要
目的探讨妊娠期妇女血清乙肝病毒DNA表达载量与前S1抗原表达的相关性。方法选取2015年4月-2017年4月在内蒙古医科大学附属医院进行乙肝病毒检测的妊娠期妇女560例为研究对象,采用酶联免疫法对所有孕妇血清中的前S1抗原表达情况进行检测,采用实时荧光定量PCR技术对所有孕妇血清中的乙肝病毒DNA表达载量进行检测,分析乙肝病毒DNA表达载量与前S1抗原表达的相关性。结果乙肝病毒DNA阳性标本222例,乙肝病毒DNA阴性标本338例。阳性标本中前S1抗原阳性率显著高于阴性标本的阳性率(P<0. 05)。前S1抗原在乙肝病毒DNA阳性标本中的表达显著高于乙肝病毒DNA阴性样本(P<0. 05)。在乙肝病毒DNA阳性标本中,97例大三阳标本中的前S1抗原阳性率为92. 78%,而在乙肝病毒DNA阴性的标本中,2例大三阳标本中前S1抗原阳性率为50. 00%,两组数据比较差异有统计学意义(P<0. 05)。在乙肝病毒DNA阳性标本中,124例小三阳标本中的前S1抗原阳性率为58. 87%,而在乙肝病毒DNA阴性的标本中,337例小三阳标本中前S1抗原阳性率为67. 06%,两组数据比较差异无统计学意义(P>0. 05)。结论妊娠期妇女血清乙肝病毒DNA的表达与前S1抗原阳性率存在一定的相关性。HBc/HBe Ag/HBsAg三项阳性模式中前S1抗原表达与乙肝病毒DNA具有一定的相关性,而HBsAg/抗HBc/抗HBe三项阳性模式中前S1抗原与乙肝病毒DNA无明显相关性。
引文
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[2]Lei Q,Ao K,Zhang Y,et al.Prognostic factors of the short-term outcomes of patients with hepatitis B virus-associated acute-on-chronic liver failure[J].Clinics(Sao Paulo),2017,72(11):686-692.
[3]曹秀贞,张丽菊,易为,等.HBV感染与妊娠期急性脂肪肝的相关性研究[J].中华实验和临床感染病杂志(电子版),2014,(3):373-375.
[4]Portilho MM,Nabuco LC,Villela-Nogueira CA,et al.Detection of occult hepatitis B in serum and oral fluid samples[J].Mem Inst Oswaldo Cruz,2018,113(1):62-65.
[5]丁霄雁,耿慧,段彼得,等.乙型肝炎病毒感染对孕妇妊娠期肝内胆汁淤积症的影响研究[J].中华医院感染学杂志,2015,(2):433-435.
[6]Yoo S,Wang W,Wang Q,et al.A pilot systematic genomic comparison of recurrence risks of hepatitis B virus-associated hepatocellular carcinoma with low-and high-degree liver fibrosis[J].BMCMed,2017,15(1):214.
[7]王星星,徐亚林,魏勇,等.前S1抗原与甲胎蛋白检测对妊娠期糖尿病孕妇HBV感染的研究[J].中华医院感染学杂志,2015,25(24):5672-5674.
[8]Mds D,Toprak M,Allmer J.A machine learning approach for MicroRNA precursor prediction in retro-transcribing virus genomes[J].J IntegrBioinform,2016,13(5):3-10.
[9]刘佳,王翠秀.替比夫定治疗妊娠中晚期乙型肝炎病毒感染免疫耐受期高病毒血症孕妇的效果及停药后丙氨酸氨基转移酶升高比例观察[J].中国妇幼保健,2017,32(15):3477-3480.
[10]Li J,He J,Fu Y,et al.Hepatitis B virus X protein inhibits apoptosis by modulating endoplasmic reticulum stress response[J].Oncotarget,2017,8(56):96027-96034.
[11]何明,王彩英,何树新,等.HBV感染母亲妊娠期低蛋白血症对母婴影响的相关分析[J].北京医学,2016,38(10):1047-1049.
[12]Okoror LE,Ajayi AO,Ijalana OB.Elevated serumβ2-microglobulin in individuals coinfected with hepatitis B and hepatitis D virus in a rural settings in Southwest Nigeria[J].BMC Res Notes,2017,10(1):719.
[13]付冬,马小艳,刘敏,等.89例慢性乙型肝炎妊娠妇女孕期肝功能异常的临床分析[J].中华实验和临床感染病杂志(电子版),2014,8(4):556-560.
[14]Yi S,Yan Y,Xiong W,et al.Distinct clinical characteristics draw a new prognostic model for splenic marginal zone lymphoma in HBVhigh prevalent region[J].Oncotarget,2017,8(58):98757-98770.
[15]李飞凤,黄启涛,黄晨,等.妊娠期慢性乙型肝炎病毒携带者病毒载量与妊娠期糖尿病的相关性[J].实用医学杂志,2016,32(19):3117-3119.
[16]Smith JW,Kroker-Lobos MF,Lazo M,et al.Aflatoxin and viral hepatitis exposures in Guatemala:molecular biomarkers reveal a unique profile of risk factors in a region of high liver cancer incidence[J].PLoS One,2017,12(12):e0189255.
[17]闫嘉营.妊娠期乙型肝炎重叠戊型肝炎病毒感染对母婴的影响[J].中国妇幼保健,2015,30(2):207-208.
[18]Luma HN,Safb E,Okalla C,et al.Prevalence and characteristics of hepatitis delta virus infection in a tertiary hospital setting in Cameroon[J].J Clin Exp Hepatol,2017,7(4):334-339.
[19]车荣华,刘敏,杨杰莲,等.乙型肝炎病毒阳性与妊娠期糖尿病的相关性研究[J].上海交通大学学报(医学版),2016,36(11):1636-1639.
[20]Wang H,Zhou Y,Yan R,et al.Development and validation of a model for staging hepatic fibrosis for chronic hepatitis B patients with Eantigen-positive[J].Oncotarget,2017,8(58):98812-98822.