PreS1抗原和HBV血清学标志物与HBV DNA的相关性及临床意义
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摘要
目的研究前S1抗原(Pre S1)、乙型肝炎病毒核酸(HBVDNA)及HBV血清标志物(HBV-M)之间关联,探讨三者在在HBV感染诊疗的临床应用价值。方法回顾性分析2016年1月～2018年6月本院1184例HBV感染疑似患者Pre S1、HBVDNA及HBV-M的检测情况。结果大三阳组Pre S1与其他6组比较,差异均存在统计学意义(χ~2=87. 597,111.736,216.866,168.015,421.362,324.532,202.062,P <0.05);"大三阳"组HBVDNA阳性率与其他6组比较,差异均有统计学意义(χ~2=131.174,254.110,216.866,158.416,402.563,306.883,181.859,P <0.05);PreS1及HBV DNA阳性率远高于其他各组;PreS1与HBeAg的关联有统计学意义(χ~2=478.981,P <0.001),PreS1与HBVDNA的关联也有统计学意义(χ~2=909.450,P <0.001);PreS1与HBeAg诊断符合率为85.13%;Pre与HBVDNA的诊断符合率为94.93%。HBeAg阳性乙肝患者HBVDNA平均病毒载量高于HBeAg阴性乙肝患者,差异有统计学意义(P <0.05)。结论传统HBV血清学检测HBeAg与PreS1及HBV DNA具有一定的相关性,PreS1及HBV DNA更能准确的反映HBV的复制状态及传染性。
Objective To explore the clinical application value of the three parameter in the diagnosis and treatment of hepatitis B virus infection,we analysed the relationship between pre S1 antigen(PreS1), hepatitis B virus nucleic acid(HBV-DNA) and hepatitis B virus serum markers(HBV-M). Methods A retrospective analysis was conducted on the detection data of PreS1, HBV-DNA and HBV-M in 1 184 patients with suspected HBV infection in our hospital from January 2016 to June 2018. Results There was a statistical difference between the PreS1 and the other six groups(χ~2=87.597,111.736,216.866,168.015,421.362,324.532,202.062, P<0.05), and the positive rate of HBV-DNA in the "big three positive" group was significantly different from that of the other six groups(χ~2=131.174,254.110,216.866,158.416,402.563,306.883. 181.859, P<0.05), the positive rate of PreS1 and HBVDNA was much higher than that of other groups, and there was a significant association between PreS1 and HBeAg(χ~2=478.981, P<0.001), and there was a significant correlation between PreS1 and HBV-DNA(χ~2=909.450, P<0.001),the diagnostic coincidence rate between PreS1 and HBeAg was 85.13% and that between pre and HBV-DNA was 94.93%. the average viral load of HBV-DNA in patients with positive hepatitis B was higher than that in HBeAg negative hepatitis B patients(P<0.05). Conclusion the traditional serological detection of hepatitis B virus(HBV) HBeAg has certain correlation with PreS1 and HBVDNA. PreS1 and HBV-DNA can more accurately reflect the replication status and contagion of HBV.
Objective To explore the clinical application value of the three parameter in the diagnosis and treatment of hepatitis B virus infection,we analysed the relationship between pre S1 antigen(PreS1), hepatitis B virus nucleic acid(HBV-DNA) and hepatitis B virus serum markers(HBV-M). Methods A retrospective analysis was conducted on the detection data of PreS1, HBV-DNA and HBV-M in 1 184 patients with suspected HBV infection in our hospital from January 2016 to June 2018. Results There was a statistical difference between the PreS1 and the other six groups(χ~2=87.597,111.736,216.866,168.015,421.362,324.532,202.062, P<0.05), and the positive rate of HBV-DNA in the "big three positive" group was significantly different from that of the other six groups(χ~2=131.174,254.110,216.866,158.416,402.563,306.883. 181.859, P<0.05), the positive rate of PreS1 and HBVDNA was much higher than that of other groups, and there was a significant association between PreS1 and HBeAg(χ~2=478.981, P<0.001), and there was a significant correlation between PreS1 and HBV-DNA(χ~2=909.450, P<0.001),the diagnostic coincidence rate between PreS1 and HBeAg was 85.13% and that between pre and HBV-DNA was 94.93%. the average viral load of HBV-DNA in patients with positive hepatitis B was higher than that in HBeAg negative hepatitis B patients(P<0.05). Conclusion the traditional serological detection of hepatitis B virus(HBV) HBeAg has certain correlation with PreS1 and HBVDNA. PreS1 and HBV-DNA can more accurately reflect the replication status and contagion of HBV.
引文
1欧阳文婷,曾智杰,孙艳虹.HBV前S1抗原与HBV-D N A、H B V-M的关系[J].分子诊断与治疗杂志,2009,1(1):20-22.
2单万水,韩红星,单金岚,等.Pre S-1和HBV-DNA与H B V-M相关性分析[J].实用医技杂志,2005(12):1576-1578.
3周萍,郭炫,陈葳,等.乙肝患者HBV-LP与HBV-DNA表达的相关性及临床应用评价[J].临床和实验医学杂志,2013,12(19):1524-1528.
4周琳,宋成,顾园园.慢性乙型肝炎患者HBV前S1抗原与HBV-M和HBV-DNA的相关性分析[J].实用预防医学,2008(5):1586-1587.
5李彩东,吴斌,陈锡莲,等.乙型肝炎病毒前S1抗原与HBV DNA和HBV-M及肝功能的相关性探讨[J].国际检验医学杂志,2015,36(7):936-938.
6郑卫东,田彩霞,左万超.1427例乙型肝炎患者PreS1、H B V D N A和H B V-M相关性分析[J].国际检验医学杂志,2012,33(7):802-805.
7高洪亮,张清禄,李宁.乙型肝炎病毒前S 1抗原与HBVDNA定量检测相关性分析及临床应用价值[J].中国病毒学杂志,2013,3(6):455-457.
8王建华,王卫国,马黎丽,等.慢性乙型肝炎病毒感染者乙肝病毒大蛋白检测的临床意义[J].中国实验诊断学,2012,16(9):1023-1025.
9李珉珉,洪丹妮,朱勤爱,等.PreS1抗原与HBeAg联合检测用于预测HBV-DNA的水平[J].暨南大学学报:自然科学与医学版,2011,32(6):637-640.
10曾繁钰,蒋冰,谭璐.Pre S1抗原与HBV血清学标志物和HBVDNA的相关性及临床意义[J].武汉大学学报:医学版,2014,35(1):85-87.
11何美林,许红梅.乙型肝炎病毒pre S1蛋白研究进展[J].儿科药学杂志,2018,24(2):49-52.
12 Casillas R,Tabernero D,Gregori J,et al.Analysis of hepatitis B virus pre S1 variability and prevalence of the rs2296651 polymorphism in a Spanish population[J].World J Gastroenterol,2018,24(6):680-692.
13 Lee S A,Lee S Y,Choi Y M,et al.Sex disparity in viral load,inflammation and liver damage in transgenic mice carrying full hepatitis B virus genome with the W4P mutation in the preS1region[J].World J Gastroenterol,2018,24(10):1084-1092.
2单万水,韩红星,单金岚,等.Pre S-1和HBV-DNA与H B V-M相关性分析[J].实用医技杂志,2005(12):1576-1578.
3周萍,郭炫,陈葳,等.乙肝患者HBV-LP与HBV-DNA表达的相关性及临床应用评价[J].临床和实验医学杂志,2013,12(19):1524-1528.
4周琳,宋成,顾园园.慢性乙型肝炎患者HBV前S1抗原与HBV-M和HBV-DNA的相关性分析[J].实用预防医学,2008(5):1586-1587.
5李彩东,吴斌,陈锡莲,等.乙型肝炎病毒前S1抗原与HBV DNA和HBV-M及肝功能的相关性探讨[J].国际检验医学杂志,2015,36(7):936-938.
6郑卫东,田彩霞,左万超.1427例乙型肝炎患者PreS1、H B V D N A和H B V-M相关性分析[J].国际检验医学杂志,2012,33(7):802-805.
7高洪亮,张清禄,李宁.乙型肝炎病毒前S 1抗原与HBVDNA定量检测相关性分析及临床应用价值[J].中国病毒学杂志,2013,3(6):455-457.
8王建华,王卫国,马黎丽,等.慢性乙型肝炎病毒感染者乙肝病毒大蛋白检测的临床意义[J].中国实验诊断学,2012,16(9):1023-1025.
9李珉珉,洪丹妮,朱勤爱,等.PreS1抗原与HBeAg联合检测用于预测HBV-DNA的水平[J].暨南大学学报:自然科学与医学版,2011,32(6):637-640.
10曾繁钰,蒋冰,谭璐.Pre S1抗原与HBV血清学标志物和HBVDNA的相关性及临床意义[J].武汉大学学报:医学版,2014,35(1):85-87.
11何美林,许红梅.乙型肝炎病毒pre S1蛋白研究进展[J].儿科药学杂志,2018,24(2):49-52.
12 Casillas R,Tabernero D,Gregori J,et al.Analysis of hepatitis B virus pre S1 variability and prevalence of the rs2296651 polymorphism in a Spanish population[J].World J Gastroenterol,2018,24(6):680-692.
13 Lee S A,Lee S Y,Choi Y M,et al.Sex disparity in viral load,inflammation and liver damage in transgenic mice carrying full hepatitis B virus genome with the W4P mutation in the preS1region[J].World J Gastroenterol,2018,24(10):1084-1092.