miRNA-329和Tiam1在胃癌中的表达及其临床意义
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摘要
目的探讨胃癌组织和癌旁正常组织中miRNA-329(miR-329)、Tiam1的表达及其临床意义。方法收集2015年6月至2017年3月在该院接受治疗的36例胃癌组织和癌旁组织标本,利用实时荧光定量PCR检测miR-329、Tiam1 mRNA的表达,并对两者进行相关性分析,研究miR-329、Tiaml与临床病理特征的关系。结果胃癌组织的miR-329水平比癌旁组织低,差异有统计学意义(P<0.05);胃肠癌组织Tiam1 mRNA水平比癌旁组织高,差异有统计学意义(P<0.05)。miR-329与Tiam1 mRNA水平呈明显负相关(r=-0.854,P<0.01)。Ⅲ期、Ⅳ期胃癌组织中miR-329水平明显低于Ⅰ期和Ⅱ期(P<0.05);Ⅲ期、Ⅳ期胃癌组织中Tiam1 mRNA水平明显高于Ⅰ期和Ⅱ期(P<0.05)。miR-329在有淋巴结转移胃癌组织的表达明显低于无淋巴结转移者(P<0.05),而有淋巴结转移胃癌组织中Tiam1 mRNA表达水平明显高于无淋巴结转移者(P<0.05)。结论 miR-329及Tiam1可能是胃癌的诊断标志物和潜在治疗靶点。
Objective To investigate the expression of microRNA-329(miR-329)and Tiam1 in gastric cancer tissues and the para-carcinoma tissues,and analyze their clinical significances.Methods A total of 36 samples of gastric cancer tissues and the para-carcinoma tissues from whom treated at the hospital from June2015 to March 2017 were collected.The expressions of miR-329 and Tiam1 mRNA were detected by real-time fluorescence quantitative PCR.The correlation between them and their correlations with the clinical pathology features were analyzed.Results miR-329 expression was lower in gastric cancer tissues compared with that in the para-carcinoma tissues,the difference was statistically significant(P<0.05).Tiam1 mRNA was significantly higher in gastric cancer tissues compared with that in the para-carcinoma tissues,the difference was statistically significant(P<0.05).miR-329 expression was negatively correlated with Tiam1 mRNA expression(r=-0.854,P<0.01).Furthermore,miR-329 expressions in gastric cancer tissues in stage Ⅲ and Ⅳ were significantly lower than those in stageⅠandⅡ(P<0.05),while Tiam1 mRNA expressions in gastric cancer tissues in stage Ⅲand Ⅳ were significantly higher than those in stageⅠandⅡ(P<0.05).In addition,miR-329 expression in gastric cancer tissues with lymph node metastasis was obviously lower than that without lymph node metastasis(P<0.05),while Tiam1 mRNA expression was obviously higher(P<0.05).Conclusion miR-29 band Tiam1 might be the potential diagnostic markers and therapeutic targets for gastric cancer.
Objective To investigate the expression of microRNA-329(miR-329)and Tiam1 in gastric cancer tissues and the para-carcinoma tissues,and analyze their clinical significances.Methods A total of 36 samples of gastric cancer tissues and the para-carcinoma tissues from whom treated at the hospital from June2015 to March 2017 were collected.The expressions of miR-329 and Tiam1 mRNA were detected by real-time fluorescence quantitative PCR.The correlation between them and their correlations with the clinical pathology features were analyzed.Results miR-329 expression was lower in gastric cancer tissues compared with that in the para-carcinoma tissues,the difference was statistically significant(P<0.05).Tiam1 mRNA was significantly higher in gastric cancer tissues compared with that in the para-carcinoma tissues,the difference was statistically significant(P<0.05).miR-329 expression was negatively correlated with Tiam1 mRNA expression(r=-0.854,P<0.01).Furthermore,miR-329 expressions in gastric cancer tissues in stage Ⅲ and Ⅳ were significantly lower than those in stageⅠandⅡ(P<0.05),while Tiam1 mRNA expressions in gastric cancer tissues in stage Ⅲand Ⅳ were significantly higher than those in stageⅠandⅡ(P<0.05).In addition,miR-329 expression in gastric cancer tissues with lymph node metastasis was obviously lower than that without lymph node metastasis(P<0.05),while Tiam1 mRNA expression was obviously higher(P<0.05).Conclusion miR-29 band Tiam1 might be the potential diagnostic markers and therapeutic targets for gastric cancer.
引文
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[23]曹苏娟,李国亮,张忠山,等.胃癌组织中Tiam1的表达及临床意义[J].实用癌症杂志,2012,27(4):343-345.
[24]LIU L,WU D H,DING Y Q.Tiam1gene expression and its significance in colorectal carcinoma[J].World J Gastroenterol,2005,11(5):705-707.
[2]MINGUEZ B,LACHENMAYER A.Diagnostic and prognostic molecular markers in hepatocellular carcinoma[J].Dis Markers,2011,31(3):181-190.
[3]HARTGRINK H H,JANSEN E P,VAN GRIEKEN NC,et al.Gastric cancer[J].Lancet,2009,374(9688):477-490.
[4]GOTODA T,YAMAMOTO H,SOETIKNO R M.Endoscopic submucosal dissection of early gastric cancer[J].JGastroenterol,2006,41(10):929-942.
[5]LI C,LI J F,CAI Q,et al.MiRNA-199a-3p:apotential circulating diagnostic biomarker for early gastric cancer[J].J Surg Oncol,2013,108(2):89-92.
[6]SEKAR D,HAIRUL ISLAM V I,THIRUGNANASAM-BANTHAM K,et al.Relevance of miR-21in HIV and non-HIV-related lymphomas[J].Tumour Biol,2014,35(9):8387-8393.
[7]SARAVANAN S,THIRUGNANASAMBANTHAM K,HANIEH H,et al.miRNA-24and miRNA-466i-5p controls inflammation in rat hepatocytes[J].Cell Mol Immunol,2015,12(1):113-115.
[8]SEKAR D,SARAVANAN S,KARIKALAN K,et al.Role of microRNA 21in mesenchymal stem cell(MSC)differentiation:apowerful biomarker in MSCs derived cells[J].Curr Pharm Biotechnol,2015,16(1):43-48.
[9]HE L,THOMSON J M,HEMANN M T,et al.A microRNApolycistron as a potential human oncogene[J].Nature,2005,435(743):828-833.
[10]SHIMONO Y,ZABALA M,CHO R W,et al.Downregulation of miRNA-200clinks breast cancer stem cells with normal stem cells[J].Cell,2009,138(3):592-603.
[11]LI Z,YU X,WANG Y,et al.By downregulating TIAM1expression,microRNA-329suppresses gastric cancer invasion and growth[J].Oncotarget,2015,6(19):17559-17569.
[12]SHI Y L,MIAO R Z,CHENG L,et al.Up-regulation of T-lymphoma and metastasis gene 1in gastric cancer and its involvement in cell invasion and migration[J].Chin Med J(Engl),2013,126(4):640-645.
[13]SOBIN L H,FLEMING I D.TNM classification of malignant tumors,fifth edition(1997).union Internationale contre le cancer and the American joint committee on cancer[J].Cancer,1997,80(9):1803-1804.
[14]HEISE K,BERTRAN E,ANDIA M E,et al.Incidence and survival of stomach cancer in a high-risk population of Chile[J].World J Gastroenterol,2009,18(15):1854-1862.
[15]WU C W,HSIUNG C A,LO S S,et al.Nodal dissection for patients with gastric cancer:a randomised controlled trial[J].Lancet Oncol,2006,7(4):309-315.
[16]WU C W,LO S S,SHEN K H,et al.Surgical mortality,survival,and quality of life after resection for gastric cancer in the elderly[J].World J Surg,2000,24(4):465-472.
[17]FEI B J,WU H R.MiR-378inhibits progression of human gastric cancer MGC-803cells by targeting MAPK1in vitro[J].Oncol Res,2012,20(12):557-564.
[18]YE Z,JING Z L,YANGBO L,et al.Propofol inhibits proliferation and invasion of osteosarcoma cells by regulation of microRNA-143expression[J].Oncol Res,2013,21(4):201-207.
[19]WANG Z Y,WANG N,LIU P X,et al.MicroRNA-25regulates chemoresistance-associated autophagy in breast cancer cells,aprocess modulated by the natural autophagy inducer isoliquiritigenin[J].Oncotarget,2014,5(16):7013-7026.
[20]YANG H F,LI Q,ZHAO W H,et al.miR-329suppresses the growth and motility of neuroblastoma by targeting KDM1A[J].FEBS Lett,2014,588(1):192-197.
[21]XIAO B X,TAN L,HE B F,et al.MiRNA-329targeting E2F1inhibits cell proliferation in glioma cells[J].JTransl Med,2013,11(1):172.
[22]WANG X J,LU X X,ZHANG T,et al.Mir-329restricts tumor growth by targeting grb2in pancreatic cancer[J].Oncotarget,2016,7(16):21441-21453.
[23]曹苏娟,李国亮,张忠山,等.胃癌组织中Tiam1的表达及临床意义[J].实用癌症杂志,2012,27(4):343-345.
[24]LIU L,WU D H,DING Y Q.Tiam1gene expression and its significance in colorectal carcinoma[J].World J Gastroenterol,2005,11(5):705-707.