化学发光技术检测乙肝前S1蛋白的临床价值
|
摘要
目的:采用化学发光技术检测乙肝前S1蛋白(pre-S1)和乙肝两对半,探讨乙肝pre-S1作为反映乙肝病毒感染、复制、传染性及肝细胞损伤指标的临床意义。方法:采用化学发光技术检测337例乙肝表面抗原阳性患者的血清乙肝两对半和pre-S1,比较不同乙肝两对半模式患者pre-S1的阳性率。结果:乙肝表面抗原的S/CO值高低与pre-S1有关,乙肝表面抗原S/CO值越高,pre-S1的阳性率越高,不同两对半模式pre-S1阳性率不同,其中以"大三阳"[HBsAg(+)/HBeAg(+)/HBcAb(+)]的阳性率最高(93.3%)。乙肝e抗原与pre-S1结果差异有显著性(P<0.01)。结论:化学发光技术检测前S蛋白能较早以及较准确地反映乙肝病毒复制的情况,对于条件不适合做乙肝病毒DNA的实验室,pre-S1是乙肝两对半的补充。
Objective:To explore the clinical value of determination of hepatitis B virus(HBV) pre-S1 in detection of HBV replication,infectivity,and liver cell injury with chemiluminescent assay.Methods:Chemiluminescent assay was used to determine serum levels of pre-S1 protein and HBV-M(HBsAg,HBsAb,HBeAg,HBeAb,HBcAb) in 337 HBsAg positive cases,then the levels of pre-S1 protein in different HBV-M groups were compared.Results:S/CO value of HBsAg was related to the level of pre-S1 protein,as it was increased with the increase of the pre-S1 protein level.The positive rate of pre-S1 was different in different modality of two pair semi-hepatitis B,and it was highest(93.3%) in patients with HBsAg(+)/HBeAg(+)/HBcAb(+).There was a significant difference between HBeAg and HBV pre-S1 protein(P<0.01).Conclusions:HBV pre-S1 protein detected with chemiluminescent assay could reflect the replication of HBV earlier and more correctly.Therefore,in the lab where HBV-DNA detection is not available,HBV pre-S1 protein is a complement for HBV-M.
Objective:To explore the clinical value of determination of hepatitis B virus(HBV) pre-S1 in detection of HBV replication,infectivity,and liver cell injury with chemiluminescent assay.Methods:Chemiluminescent assay was used to determine serum levels of pre-S1 protein and HBV-M(HBsAg,HBsAb,HBeAg,HBeAb,HBcAb) in 337 HBsAg positive cases,then the levels of pre-S1 protein in different HBV-M groups were compared.Results:S/CO value of HBsAg was related to the level of pre-S1 protein,as it was increased with the increase of the pre-S1 protein level.The positive rate of pre-S1 was different in different modality of two pair semi-hepatitis B,and it was highest(93.3%) in patients with HBsAg(+)/HBeAg(+)/HBcAb(+).There was a significant difference between HBeAg and HBV pre-S1 protein(P<0.01).Conclusions:HBV pre-S1 protein detected with chemiluminescent assay could reflect the replication of HBV earlier and more correctly.Therefore,in the lab where HBV-DNA detection is not available,HBV pre-S1 protein is a complement for HBV-M.
引文
1 Hsieh YH,Su IJ,Wang HC,et al.Pre2S mutant surface antigens in chronic hepatitis B virus infection induce oxidative stress and DNA damage.Carcinogenesis,2004,25:2023-2032.
2董运启.乙型肝炎病毒前S1蛋白检测的临床价值.国际检验医学杂志,2012,(S1),20-21
3张和平,林国跃,王萍,等.HBV前S-2蛋白检测与HBV-DNA和乙肝二对半模式相关性研究.医学综述,2011,(01):520-522
4杨晋荣,杨栋梁.HBeAg阴性HBV感染者不同病毒载量与前S1抗原和大蛋白关系研究.临床合理用药杂志,2011,(35):12-13
5 Funk ML,Rosenberg DM,Lok AS.World wide epidemic of HBeAg negative chronic hepatitis B and associated precore and core promoter variants J.Viral Hepat,2002,9:52-61.
6张学英,李励,柴晓文.乙型肝炎病毒标志物不同模式与HBVDNA、PA、ADA及ALT浓度的相关性研究.临床和实验医学杂志,2012,(6):17-18
7黄启强,黄丽庆,周晓兰,等.乙型肝炎病毒前S1抗原与传统标志物相关性分析.检验医学与临床,2011,(11):1341-1343
2董运启.乙型肝炎病毒前S1蛋白检测的临床价值.国际检验医学杂志,2012,(S1),20-21
3张和平,林国跃,王萍,等.HBV前S-2蛋白检测与HBV-DNA和乙肝二对半模式相关性研究.医学综述,2011,(01):520-522
4杨晋荣,杨栋梁.HBeAg阴性HBV感染者不同病毒载量与前S1抗原和大蛋白关系研究.临床合理用药杂志,2011,(35):12-13
5 Funk ML,Rosenberg DM,Lok AS.World wide epidemic of HBeAg negative chronic hepatitis B and associated precore and core promoter variants J.Viral Hepat,2002,9:52-61.
6张学英,李励,柴晓文.乙型肝炎病毒标志物不同模式与HBVDNA、PA、ADA及ALT浓度的相关性研究.临床和实验医学杂志,2012,(6):17-18
7黄启强,黄丽庆,周晓兰,等.乙型肝炎病毒前S1抗原与传统标志物相关性分析.检验医学与临床,2011,(11):1341-1343