甘蔗与斑茅割手密复合体的回交后代真实性鉴定及染色体遗传分析
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摘要
【目的】掌握斑茅割手密复合体(简称斑割复合体)在杂交利用过程中的染色体遗传规律,为斑割复合体适宜回交利用代数提供参考依据。【方法】选用4对引物对甘蔗与斑茅割手密复合体的回交后代进行SSR标记鉴定,并利用甘蔗根尖细胞酶解去壁低渗法对真实杂种进行染色体数目观察。【结果】供试的28个杂交后代材料(15个GT05-164与GXASF_108-2-28杂交BC_1代,13个GT42与GXASBC_112-A6-25杂交BC_2代)均为真实杂种。选择在2对SSR引物中均扩增出父本特征带的后代(5个BC_1代和7个BC_2代)进行染色体观察,结果发现,5个BC_1代的染色体数目介于95~98条,其染色体按"n+n"方式传递;7个BC_2代的染色体数目介于94~101条,其染色体也按"n+n"方式传递。【结论】甘蔗与斑割复合体杂交BC_1和BC_2代中染色体传递方式均为"n+n",母本甘蔗的染色体未加倍,高贵化育种进程减慢,可能需要更高的杂交代数才能获得聚集斑茅和割手密血缘的优良品种(系)。
【Objective】Inheritance of chromosome was studied in the process of hybridization between sugarcane(Saccharum officinarum) and intergeneric hybrid complex(Erianthus arundinaceus×Saccharum spontaneum) to lay a good foundation for exploration of optimum backcross generations.【Method】The backcross progenies from sugarcane(S.officinarum)and intergeneric hybrid(E.arundinaceusxS.spontaneum) were identified with four pairs of SSR primers and chromosome number for the hybrids was calculated by means of cell wall degradation hypotonic smear method.【Result】All 28 progenies,15 BC_1 progenies from GT05-164 and GXASF_108-2-28 and 13 BC_2 progenies from GT42 and GXASBC_112-A6-25,were confirmed as hybrids.Chromosome numbers of progenies(five BC_1 hybrids and seven BC_2 hybrids) in which amplification of paternal characteristics band occurred in two pairs of SSR primers were observed.The somatic chromosome numbers of five BC_ hybrids and seven BC_2 hybrids were 95-98 and 94-101,respectively,all following the law of n+n.【Conclusion】 Chromosome transmission in BC_1 and BC_2 hybrids of backcross progeny from sugarcane and intergeneric hybrid follows n+n mode.Chromosome numbers female parent sugarcane(S.officinarum) are not doubled,and nobilisation progress slows dowa More generations may be needed to obtain superior varieties(lines) of E.arundinaceus and S.spontaneum consanguinity.
【Objective】Inheritance of chromosome was studied in the process of hybridization between sugarcane(Saccharum officinarum) and intergeneric hybrid complex(Erianthus arundinaceus×Saccharum spontaneum) to lay a good foundation for exploration of optimum backcross generations.【Method】The backcross progenies from sugarcane(S.officinarum)and intergeneric hybrid(E.arundinaceusxS.spontaneum) were identified with four pairs of SSR primers and chromosome number for the hybrids was calculated by means of cell wall degradation hypotonic smear method.【Result】All 28 progenies,15 BC_1 progenies from GT05-164 and GXASF_108-2-28 and 13 BC_2 progenies from GT42 and GXASBC_112-A6-25,were confirmed as hybrids.Chromosome numbers of progenies(five BC_1 hybrids and seven BC_2 hybrids) in which amplification of paternal characteristics band occurred in two pairs of SSR primers were observed.The somatic chromosome numbers of five BC_ hybrids and seven BC_2 hybrids were 95-98 and 94-101,respectively,all following the law of n+n.【Conclusion】 Chromosome transmission in BC_1 and BC_2 hybrids of backcross progeny from sugarcane and intergeneric hybrid follows n+n mode.Chromosome numbers female parent sugarcane(S.officinarum) are not doubled,and nobilisation progress slows dowa More generations may be needed to obtain superior varieties(lines) of E.arundinaceus and S.spontaneum consanguinity.
引文
高轶静,方锋学,刘昔辉,张荣华,宋焕忠,杨荣仲,罗霆,段维兴,游建华,张革民.2012.甘蔗与斑茅割手密复合体杂交后代的分子标记鉴定[J].植物遗传资源学报,13(5):912-916.Gao Y J,Fang F X,Liu X H,Zhang R H,Song H Z,Yang R Z,Luo T,Duan W X,You J H,Zhang G M.2012.Identification of progeny from crosses between sugarcane(Saccharum spp.)and intergeneric hyhrid complex(Erianthus arundinaceus×Saccharum spontaneum)with molecular markers[J].Journal of Plant Genetic Resources,13(5):912-916.
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黄玉新,罗霆,刘昔辉,周珊,杨翠芳,高轶静,杨翠凤,段维兴,周会,张革民,李杨瑞.2016.甘蔗与斑茅割手密复合体(GXAS07-6-1)杂交后代的染色体遗传分析[J].热带作物学报,37(2):220-225.Huang Y X,Luo T,Liu X H,Zhou S,Yang C F,Gao Y J,Yang C F,Duan W X,Zhou H,Zhang G M,Li Y R.2016.Genetic analysis of chromosomes for the progeny between sugarcane(Saccharum spp.)and intergeneric hybrid complex(Erianthus arundinaceusxSaccharum spontaneum)[J].Chinese Journal of Tropical Crops,37(2):220-225.
廖芩,黄河星,黄兴,杨善,莫俊杰,周鸿凯.2015.甘蔗物理诱变M2代株系的ISSR分析[J].江西农业学报,27(7):28-30.Liao Q,Huang H X,Huang X,Yang S,Mo J J,Zhou H K.2015.ISSR analysis of physically-mutated sugarcane strains in M2 generation[J].Acta Agriculturae Jiangxi,27(7):28-30.
林秀琴,陆鑫,毛钧,刘新龙,苏火生,蔡青.2013.甘蔗属热带种与滇蔗茅远缘杂交F_1代GISH分析[J]_西南农业学报,26(4):1327-1331.Lin X Q,Lu X,Mao J,Liu X L,Su H S.Cai Q.2013.GISH analysis of intergeneric F_1 hybrids between Saccharum ofGcinarum and Erianthus rockii[J].Southwest China Journal of Agricultural Sciences,26(4):1327-1331.
刘文荣,邓祖湖,张木清,卓晓蕾,符成,张垂明.2004.甘蔗斑茅的杂交利用及其杂种后代鉴定系列研究Ⅲ.甘蔗斑茅远缘杂交后代细胞遗传分析[J].作物学报,30(11):1093-1096.Liu W R.Deng Z H,Zhang M Q,Zhuo X L,Fu C,Zhang C M.2004.Use and characterization of the genuine intergeneric hybrids from the cross of Saccharum spp.and .arundinaceum Retz.Ⅲ.Cytogenetic analysis for the hybrid and backcross progeny of S.officinarum L.and Erianthus sect.Ripidium[J].Acta Agronomica Sinica,30(11):1093-1096.
刘昔辉,方锋学,高轶静,张荣华,宋焕忠,杨荣仲,方位宽,段维兴,罗霆,张革民,李杨瑞.2012a.斑茅割手密杂种后代真实性鉴定及遗传分析[J].作物学报,38(5):914-920.Liu X H,Fang F X,Gao Y J,Zhang R H,Song H Z,Yang R Z,Fang W K,Duan W X,Luo T,Zhang G M,Li Y R.2012a.Identification and genetic analysis of hybrid from cross between Erianthus arundinacius(Retz.)Jesws.and Saccharum spontaneum L.[J].Acta Agronomica Sinica,38(5):914-920.
刘昔辉,方锋学,张荣华,宋焕忠,杨荣仲,高轶静,区惠平,雷敬超,罗霆,段维兴,张革民,李杨瑞.2012b.甘蔗与河八王属间杂种的SSR标记鉴定[J].西南农业学报,25(1):38-43.Liu X H,Fang F X,Zhang R H,Song H Z,Yang R Z,Gao Y J,Ou H P,Lei J C,Luo T,Duan W X,Zhang G M,Li Y R.2012b.Identification of progenies from Sugarcane×Narenga porphyrocoma(Hance)Bor.by SSR marker[J].Southwest China Journal of Agricultural Sciences,25(1):38-43.
刘许辉.2013.甘蔗与斑茅割手密复合体杂交后代的遗传分析[D].南宁:广西大学.Liu X H.2013.Genetic analysis for the progenies from the cross Saccharum with the hybrid of Erianthus arundinaceus(Retz.)Jeswiet and Saccharum spontaneum L[D].Nanning:Guangxi University.
陆鑫,毛钧.刘洪博.刘新龙,马丽,苏火生,蔡青.2012.甘蔗野生种滇蔗茅种质创新利用研究Ⅰ.甘蔗与滇蔗茅远缘杂交F_1群体构建与SSR分子标记鉴定[J].植物遗传资源学报,13(2):321-324.Lu X,Mao J,Liu H B,Liu X L,Ma L,Su H S,Cai Q.2012.Innovation germplasm of Erianthus rockii I.Establishment of F_1 intergeneric distant hybridization of Saccharum officinarum L.and Erianthus mckii and the identification of F_1by SSR molecular markers[J].Journal of Plant Genetic Resources,13(2):321-324.
桃联安,楚连璧,经艳芬,刘新龙,安汝尔.董立华,杨李和,周清明.殷惠芬.2009.云南割手密82-114种间杂交后代SSR分子标记鉴定[J].植物遗传资源学报.10(1):132-135.Tao L A,Chu L B,Jing Y F,Liu X L,An R D,Dong L H,Yang L H,Zhou Q M,Duan H F.2009.Identification of genuine hybrids from the cross of chewing cane and Saccharum spontaneum in Yunnan by SSR markers[J].Journal of Plant Genetic Resources,10(1):132-135.
王先宏,李富生,何丽莲,娄红波,杨清辉,何顺长.2014.甘蔗与蔗茅杂交F_2BC_1代的染色体遗传分析[J].中国农学通报,30(15):81-85.Wang X H,Li F S,He L L,Lou H B,Yang Q H,He S C.2014.Chromosomal transmission of F_2BC_1 progenies between sugarcane and Erianthus fulvus[J].Chinese Agricultural Science Bulletin,30(15):81-85.
吴嘉云.2013.甘蔗与斑茅后代染色体遗传分析及抗性初步评价[D].福州:福建农林大学.Wu J Y.2013.The chromosome inheritance for the hybrid progeny of S.officinarum L.and E.arundinaceum and germplasm resistance preliminary evaluation[D].Fuzhou:Fujian Agricultural and Forestry University.
杨翠凤,张春雅,杨丽涛,李杨瑞.2015.不同基因型甘蔗种质资源的表型遗传多样性[J].广西植物,35(4):556-563.Yang C F,Zhang C Y,Yang L T,Li Y R.2015.Phenotypic and genetic diversity of sugarcane(Saccharum L.)protospecies and landraces[J].Guihaia,35(4):556-563.
阴云伙,温和楦,陈龙,程攀,曲姗姗,田发春,彭炳生,吴帅,李土明,周卫营.2015.利用SSR标记鉴定两系杂交稻“广两优7203”的种子纯度[J].江西农业学报,27(11):20-22.Yin Y H,Wen H X,Chen L,Cheng P,Qu S S,Tian F C,Peng B S,Wu S,Li T M,Zhou W Y.2015.Identification of seed purity of two-line hybrid rice"Guangliangyou 7203"by using SSR markers[J].Acta Agriculturae Jiangxi,27(11):20-22.
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