斑茅割手密复合体(GXAS07-6-1)及其与甘蔗F_1的GISH分析
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摘要
斑茅割手密复合体(GXAS07-6-1)是广西蔗茅属斑茅和广西甘蔗属割手密的属间杂种,聚集了双亲的优点。本研究利用基于Alu-like的PCR鉴定方法对GXAS07-6-1及甘蔗与GXAS07-6-1的3份F_1材料进行真实性鉴定,基于基因组原位杂交技术(GISH)对父本GXAS07-6-1及其3份F_1染色体组成及核型进行分析。研究结果表明:3份F_1材料为GXAS07-6-1的真杂种;父本GXAS07-6-1的染色体众数为62条,其中30条来自蔗茅属斑茅,32条来自甘蔗属割手密,核型分类属于1B,其染色体按"n+n"方式传递;GXASF_108-2-17、GXASF_108-2-22、GXASF_108-2-32的染色体数目为78~80条,其中69~71条来自甘蔗属,9~11条来自蔗茅属斑茅,3份F_1的核型分类分别属于2B、1B、1B,染色体传递方式均为"n+n"。父本GXAS07-6-1及3份F_1材料中均未发现有染色体的交换或易位现象。甘蔗与斑割复合体杂交,蔗茅属斑茅染色体在亲子间传递过程存在丢失现象。
GXAS07-6-1,an intergeneric hybrid of Erianthus arundinaceus(Retz.) Jeswiet and Saccharum spontaneum L.has the advantages of its parents.In this study,the turth and fales of three F_1 hybrids progenies GXAS08-2-17,GXAS08-2-22 and GXAS08-2-32 were identified by the PCR method of Alu-like.The chromosomes composi-tion and karyotype of the GXAS07-6-1 and the F_1 generation were analyzed using the genomic in situ hybridization(GISH) technique.The results showed that the three F_1 materials were true progenies.There were 62 chromosomes in GXAS07-6-1,of which 30 were from E.arundinaceus(Retz.) Jeswiet and 32 were from S.spontaneum L..and the somatic chromosome karyotypic was 1B,following n+n transmission.Furthermore,the chromosomes numbers of GXASF_108-2-17,GXASF_108-2-22 and GXASF_108-2-32 ranged from 78 to 80,of which 69-71 were from Saccharum spp.and 9 to 11 were from E.arundinaceus(Retz.) Jeswiet.The somatic chromosome karyotypes of three F_1 progeny were 2B,1B,1B,respectively,and all of the three progenies following n+n transmission.Chromosome translocation and exchange were not observed in the intergeneric hybrid GXAS07-6-1 and its F_1 hybrids as well.Chromosome missing also was confirmed from E.arundinaceus(Retz.) Jeswiet in the F_1 progeny.
GXAS07-6-1,an intergeneric hybrid of Erianthus arundinaceus(Retz.) Jeswiet and Saccharum spontaneum L.has the advantages of its parents.In this study,the turth and fales of three F_1 hybrids progenies GXAS08-2-17,GXAS08-2-22 and GXAS08-2-32 were identified by the PCR method of Alu-like.The chromosomes composi-tion and karyotype of the GXAS07-6-1 and the F_1 generation were analyzed using the genomic in situ hybridization(GISH) technique.The results showed that the three F_1 materials were true progenies.There were 62 chromosomes in GXAS07-6-1,of which 30 were from E.arundinaceus(Retz.) Jeswiet and 32 were from S.spontaneum L..and the somatic chromosome karyotypic was 1B,following n+n transmission.Furthermore,the chromosomes numbers of GXASF_108-2-17,GXASF_108-2-22 and GXASF_108-2-32 ranged from 78 to 80,of which 69-71 were from Saccharum spp.and 9 to 11 were from E.arundinaceus(Retz.) Jeswiet.The somatic chromosome karyotypes of three F_1 progeny were 2B,1B,1B,respectively,and all of the three progenies following n+n transmission.Chromosome translocation and exchange were not observed in the intergeneric hybrid GXAS07-6-1 and its F_1 hybrids as well.Chromosome missing also was confirmed from E.arundinaceus(Retz.) Jeswiet in the F_1 progeny.
引文
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[3]刘少谋,符成,陈勇生.近十年海南甘蔗育种场斑茅后代回交利用研究[J].甘蔗糖业,2007(4):1-6
[4]符成,邓海华,杨业后,等.斑茅及其杂种后代主要经济性状研究[J].甘蔗糖业,2004(4):1-5
[5]王丽萍,蔡青,范源洪,等.甘蔗(Saccharum)与斑茅(Erianthus arundinaceus)远缘杂交利用研究[J].西南农业学报,2007,20(4):721-726
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[7]高轶静,方锋学,刘昔辉,等.甘蔗与斑茅割手密复合体杂交后代的分子标记鉴定[J].植物遗传资源学报,2012,13(5):912-916
[8]黄玉新,罗霆,刘昔辉,等.甘蔗与斑茅割手密复合体(GXAS07-6-1)杂交后代的染色体遗传分析[J].热带作物学报,2016,37(2):220-225
[9]Alix K,Paulet F,Glaszmann J C,et al.Interspecies-specific sequences in the Saccharum complex[J].Theor Appl Genet,1999,99:962-968
[10]林秀琴,陆鑫,毛钧.甘蔗属热带种与滇蔗茅远缘杂交F1代GISH分析[J].西南农业学报,2013,26(4):1327-1331
[11]李懋学,陈瑞阳.关于植物核型分析的标准化问题[J].武汉植物学研究,1985,3(4):297-302
[12]黄玉新.斑茅割手密复合体(GXAS07-6-1)及其与甘蔗杂交后代的分子细胞遗传学研究[D].南宁:广西大学,2016
[13]陈健文,Piperidis N,李奇伟,等.用基因组原位杂交方法分析甘蔗-斑茅杂种及回交后代的染色体组成[J].分子植物育种,2012,8(2):293-296
[14]黄永吉,吴嘉云,刘少谋,等.基于GISH的甘蔗与斑茅F1染色体遗传与核型分析[J].植物遗传资源学报,2014,15(2):394-398
[15]Stebbins G L.Chromosomal evolution in higher plants[M].London:Edward Arnold,1971:87-89
[16]洪德元.植物细胞分类学[M].北京:科学出版社,1990
[17]刘文荣,邓祖湖,张木清,等.甘蔗斑茅的杂交利用及其杂种后代鉴定系列研究Ⅲ.甘蔗斑茅远缘杂交后代细胞遗传分析[J].作物学报,2004,30(11):1093-1096
[18]邓祖湖,李玉蝉,刘文荣,等.甘蔗和斑茅远缘杂交后代的染色体遗传分析[J].热带作物学报,2007,28(3):62-67
[19]吴嘉云.甘蔗与斑茅杂交后代染色体遗传分析及抗性初步评价[D].福州:福建农林大学,2013
[20]黄永吉,符成,林炜乐,等.3个甘蔗与斑茅远缘杂交后代BC1的染色体遗传分析[J].热带作物学报,2015,36(1):53-58
[2]Piperidis N,Chen J W,Deng H H,et al.GISH characterization of Erianthus arundinaceus chromosomes in three generations of sugar cane intergeneric hybrids[J].Genome,2010,53:331-336
[3]刘少谋,符成,陈勇生.近十年海南甘蔗育种场斑茅后代回交利用研究[J].甘蔗糖业,2007(4):1-6
[4]符成,邓海华,杨业后,等.斑茅及其杂种后代主要经济性状研究[J].甘蔗糖业,2004(4):1-5
[5]王丽萍,蔡青,范源洪,等.甘蔗(Saccharum)与斑茅(Erianthus arundinaceus)远缘杂交利用研究[J].西南农业学报,2007,20(4):721-726
[6]刘昔辉,方锋学高轶静,等.斑茅割手密杂种后代真实性鉴定及遗传分析[J].作物学报,2012,38(5):914-920
[7]高轶静,方锋学,刘昔辉,等.甘蔗与斑茅割手密复合体杂交后代的分子标记鉴定[J].植物遗传资源学报,2012,13(5):912-916
[8]黄玉新,罗霆,刘昔辉,等.甘蔗与斑茅割手密复合体(GXAS07-6-1)杂交后代的染色体遗传分析[J].热带作物学报,2016,37(2):220-225
[9]Alix K,Paulet F,Glaszmann J C,et al.Interspecies-specific sequences in the Saccharum complex[J].Theor Appl Genet,1999,99:962-968
[10]林秀琴,陆鑫,毛钧.甘蔗属热带种与滇蔗茅远缘杂交F1代GISH分析[J].西南农业学报,2013,26(4):1327-1331
[11]李懋学,陈瑞阳.关于植物核型分析的标准化问题[J].武汉植物学研究,1985,3(4):297-302
[12]黄玉新.斑茅割手密复合体(GXAS07-6-1)及其与甘蔗杂交后代的分子细胞遗传学研究[D].南宁:广西大学,2016
[13]陈健文,Piperidis N,李奇伟,等.用基因组原位杂交方法分析甘蔗-斑茅杂种及回交后代的染色体组成[J].分子植物育种,2012,8(2):293-296
[14]黄永吉,吴嘉云,刘少谋,等.基于GISH的甘蔗与斑茅F1染色体遗传与核型分析[J].植物遗传资源学报,2014,15(2):394-398
[15]Stebbins G L.Chromosomal evolution in higher plants[M].London:Edward Arnold,1971:87-89
[16]洪德元.植物细胞分类学[M].北京:科学出版社,1990
[17]刘文荣,邓祖湖,张木清,等.甘蔗斑茅的杂交利用及其杂种后代鉴定系列研究Ⅲ.甘蔗斑茅远缘杂交后代细胞遗传分析[J].作物学报,2004,30(11):1093-1096
[18]邓祖湖,李玉蝉,刘文荣,等.甘蔗和斑茅远缘杂交后代的染色体遗传分析[J].热带作物学报,2007,28(3):62-67
[19]吴嘉云.甘蔗与斑茅杂交后代染色体遗传分析及抗性初步评价[D].福州:福建农林大学,2013
[20]黄永吉,符成,林炜乐,等.3个甘蔗与斑茅远缘杂交后代BC1的染色体遗传分析[J].热带作物学报,2015,36(1):53-58