斑割复合体及其杂交后代的生物量及根系性状
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摘要
本研究利用前期获得的含广西斑茅和广西割手密血缘的甘蔗育种新材料及其亲本共11份无性系进行桶栽试验,分析了参试材料植株地上部生物量和根系的主要相关性状在斑茅割手密复合体杂交利用过程各世代的变化。结果表明:参试的斑茅割手密复合体及其与甘蔗杂交、回交获得的F1代材料以及BC1代在株高、茎径、单茎重和叶面积上都表现比其亲本甘蔗有更大的优势;根系重量和活力都略有增加;地上部分生物产量上均表现出超亲效应,其超亲效应与绿叶面积的超亲表现相关性显著。
Effective utilization of new genes from wild germplasm is important for broadening the genetic base of sugarcane breeding.Aboveground biomass and main characters of root in 11 clones of successive generations of intergeneric hybrid Erianthus arundinaceus × Saccharum spontaneum and their progeny were analyzed.The results showed that the plant height,stalk diameter,single stalk weight and leaf area in the intergeneric hybrids F1 and BC1 were all superior to their parents.Slight increases in weight and activity of root were also found in the hybrid lines.Aboveground biomass was increased distinctively,which displayed a significant correlation with the hybrid vigor of green leaf area.These results provide theoretical bases to improve sugarcane cultivars by utilizing superior genes in both S.spontaneum and E.arundinaceusin introgression breeding program.
Effective utilization of new genes from wild germplasm is important for broadening the genetic base of sugarcane breeding.Aboveground biomass and main characters of root in 11 clones of successive generations of intergeneric hybrid Erianthus arundinaceus × Saccharum spontaneum and their progeny were analyzed.The results showed that the plant height,stalk diameter,single stalk weight and leaf area in the intergeneric hybrids F1 and BC1 were all superior to their parents.Slight increases in weight and activity of root were also found in the hybrid lines.Aboveground biomass was increased distinctively,which displayed a significant correlation with the hybrid vigor of green leaf area.These results provide theoretical bases to improve sugarcane cultivars by utilizing superior genes in both S.spontaneum and E.arundinaceusin introgression breeding program.
引文
[1]Li Y R,Yang L T.Sugarcane agriculture and sugar industry in China[J].Sugar Tech,2015,17(1):1-8.
[2]Gouy M,Nibouche S,Hoarau J Y,Costet L.Improvement of yield per se in sugarcane[M].In:Varshney R K,Tuberosa R.Translational Genomics for Crop Breeding:Abiotic Stress,Yield,and Quality.Hoboken:John Wiley&Sons Ltd,2013:211-238
[3]李杨瑞,杨丽涛,谭宏伟,朱秋珍,王维赞,杨柳.广西甘蔗栽培技术的发展进步[J].南方农业学报,2014,45(10):1770-1775Li Y R,Yang L T,Tan H W,Zhu Q Z,Wang W Z,Yang L.Development and progress of sugarcane farming technologies in Guangxi,China[J].Journal of Southern Agriculture,2014,45(10):1770-1775(in Chinese)
[4]Waclawovsky A J,Sato P M,Lembke C G,Moore P H,Souza G M.Sugarcane for bioenergy production:an assessment of yield and regulation of sucrose content.Plant Biotechnology Journal,2010,8:263-276
[5]Tew T L.World sugarcane variety census-year 2000[J].Sugar Cane International,2003,2(March/April):12-18
[6]张木清,王华忠,白晨.糖料作物遗传改良与高效育种[M].北京:中国农业出版社,2006:1-252Zhang M Q,Wang H Z,Bai C.Genetic Improvement and Highly Efficient Breeding for Sugar Crop[M].Beijing:China Agricultural Press,2006:1-252(in Chinese)
[7]李杨瑞.现代甘蔗学[M].北京:中国农业出版社,2010:103-116.Li Y R.Modern Sugarcane Science[M].Beijing:China Agriculture Press,2010:103-106(in Chinese)
[8]Nair N V.Sugarcane varietal development programmes in India:An overview[J].Sugar Tech,2011,13(4):275-280
[9]王丽萍,马丽,夏红明,陆鑫,蔡青,范源洪,陈辉,刘新龙.甘蔗细茎野生种(S spontaneum)在杂交育种中的利用[J].中国糖料,2006,2(1):1-4Wang L P,Ma L,Xia H M,Lu X,Cai Q,Fan Y H,Chen H,Liu X L.Application of S spontaneumin sugarcane cross-breeding.Sugar Crops of China,2006,2(1):1-4(in Chinese)
[10]桃联安,经艳芬,董立华,安汝东,杨李和,周清明,段慧芬,朱建荣.云南甘蔗细茎野生种82-114测交后代主要性状遗传分析[J].植物遗传资源学报,2011,12(3):419-424Tao L A,Jing Y F,Dong L H,An R D,Yang L H,Zhou Q M,Duan H F,Zhu J R.Genetic analysis of main traits in descendants of crossing with Saccharum spontaneum 82-114in Yunnan.Journal of Plant Genetic Resources,2011,12(3):419-424(in Chinese)
[11]陈建文,Nathalie Piperidis,李奇伟,陈勇生,符成,Phillip Jackson,George Piperidis,邓海华.用基因组原位杂交方法分析甘蔗-斑茅杂种及回交后代的染色体组成[J].分子植物育种,2010,2(1):293-296Chen J W,Nathalie Piperidis,Li Q W,Chen Y S,Fu C,Phillip Jackson,George Piperidis,Deng H H.Chromosome pattern of the hybrids and their backcross progenies derived from sugarcane and Erianthus arundinaceus identified by in situ genomic hybridization.Molecular Plant Breeding,2010,2(1):293-296(in Chinese)
[12]Piperidis N,Chen J W,Deng H H,Wang L P,Jackson P,Pepiridis G.GISH characterization of Erianthus arundinaceus chromosomes in three generations of sugarcane intergeneric hybrids[J].Genome,2010,53:331-336
[13]刘少谋,符成,陈勇生.近十年海南甘蔗育种场斑茅后代回交利用研究[J].甘蔗糖业,2007(4):1-6Liu S M,Fu C,Chen Y S.Utilization of Erianthus arundinaceus progeny through backcross over the last decade at Hainan sugarcane breeding station[J].Sugarcane and Cane Sugar,2007(4):1-6(in Chinese)
[14]王丽萍,蔡青,范源洪,陆鑫,Karen Aiken,马丽,刘新龙,夏红明.甘蔗(Saccharum)与斑茅(Erianthus arundinaceus)远缘杂交利用研究[J].西南农业学报,2007,20(4):721-726Wang L P,Cai Q,Fan Y H,Lu X,Karen Aiken,Ma L,Liu X L,Xia H M.Study on the distant hybrid utilization between Saccharum and Erianthus arundinanceus[J].Southwest J Journal Agric Sci,2007,20(4):721-726(in Chinese)
[15]刘昔辉,方锋学,高轶静,张荣华,宋焕忠,杨荣仲,方位宽,段维兴,罗霆,张革民,李杨瑞.斑茅割手密杂种后代真实性鉴定及遗传分析[J].作物学报,2012,38(5):914-920Liu X H,Fang F X,Gao Y J,Zhang R H,Song H Z,Yang R Z,Fang W K,Duan W X,Luo T,Zhang G M,Li Y R.Identification and genetic analysis of hybrid from cross between Erianthus arundinacius(Retz)Jesws and Saccharum spontaneum L[J].Acta Agronomica Sinica,2012,38(05):914-920(in Chinese)
[16]高轶静,方锋学,刘昔辉,张荣华,宋焕忠,杨荣仲,罗霆,段维兴,游建华,张革民.甘蔗与斑茅割手密复合体杂交后代的分子鉴定[J].植物遗传资源学报,2012,13(5):912-916Gao Y J,Fang F X,Liu X H,Zhang R H,Song H Z,Yang R Z,Luo T,Duan W X,You J H,Zhang G M.Identification of progeny from crosses between sugarcane(Saccharum spp.)and intergeneric hybrid complex(Erianthus arundinaceus×Saccharum spontaneum)with molecular markers[J].Journal of Plant Genetic Resources,2012,13(5):912-916(in Chinese)
[17]张志良.植物生理学实验手册[M].北京:高等教育出版社,1998.33-38Zhang Z L.The Laboratory Methods of Plant Physiology[M].Beijing:Higher Education Press,1998:33-38(in Chinese)
[18]Jiang N,Di Y T,Zhao G C,Xu K Z,Wu Z H,Zhang Z A,Ling F L.Biomasses in different organs of rice cultivars developed during recent forty-seven years in Jilin Province,China[J].Rice Science,2010,17(3):206-212
[19]Tollenaar M.Genetic improvement in grain yield of commercial maize hybrids grown in Ontario from 1959to 1988[J].Crop Science,1989,29:1365-1371
[20]胡昌浩,董树亭,王空军,孙庆泉.我国不同年代玉米品种生育特性演进规律研究:Ⅱ.物质生产特性的演进[J].玉米科学,1998,6(3):49-53Hu C H,Dong S T,Wang K J,Sun Q Q.Study of growth and development evolution characteristics in maize varieties released in different years:Ⅱ.Evolution of dry matter production[J].Journal of Maize Sciences,1998,6(3):49-53(in Chinese)
[21]Brancourt H M,Doussinault G,Lecomte C,Berard P,Le B B,Trottet M.Genetic improvement of agronomic traits of winter wheat cultivars released in France from 1946to 1992[J].Crop Science,2003,43:37-45
[22]Shearman V J,Sylvester-Bradley R,Scott R K,Foulkes M J.Physiological processes associated with wheat yield progress in the UK[J].Crop Science,2005,45:175-185
[23]Cober E R,Morrison M J,Ma B,Butler G.Genetic improvement rates of short-season soybean increase with plant population[J].Crop Science,2005,45:1029-1034
[24]周鸿凯,刘桂富,蔡华斌,王尚钦,郭荣发.甘蔗生物量育种的ADGE遗传分析[J].热带亚热带植物学报,2005,13(3):259-263Zhou H K,Liu G F,Cai H B,Wang S Q,Guo R F.Genetic analysis of biomass in sugarcane using ADGE model[J].Journal of Tropical and Subtropical Botany,2005,13(3):259-263(in Chinese)
[25]Legendre B L,Burner D M.Biomass production of sugarcane cultivars and early-generation hybrids[J].Biomass Bioenergy,1995,8(2):55-61
[26]Russell R S.Plant Root System:Their Function and Interaction with the Soil[M].London:McGraw-Hill Book Co,1997:12-15
[27]De dorlodot S,Forster B,Pages L,Price A,Tuberosa R,Draye X.Root system architecture:Opportunities and constraints for genetic improvement of crops[J].Trends in Plant Science,2007,12(10):470-481
[28]程式华,曹立勇,陈深广,朱德峰,王熹,闵绍楷,翟虎渠.后期功能型超级杂交稻的概念及生物学意义[J].中国水稻科学,2005,19(3):280-284Cheng S H,Cao L Y,Chen S G,Zhu D F,Wang X,Min S K,Zhai H Q.Conception of late-stage vigor super hybrid rice and its biological significance l[J].Chinese Journal of Rice Science,2005,19(3):280-284(in Chinese)
[29]Fitter A.Characteristics and functions of root systems[M].In:Waisel Y,Eshel A,Kafkafi U,Plant Roots,the Hidden Half.New York:Marcel Dekker Inc,2002:15-32
[2]Gouy M,Nibouche S,Hoarau J Y,Costet L.Improvement of yield per se in sugarcane[M].In:Varshney R K,Tuberosa R.Translational Genomics for Crop Breeding:Abiotic Stress,Yield,and Quality.Hoboken:John Wiley&Sons Ltd,2013:211-238
[3]李杨瑞,杨丽涛,谭宏伟,朱秋珍,王维赞,杨柳.广西甘蔗栽培技术的发展进步[J].南方农业学报,2014,45(10):1770-1775Li Y R,Yang L T,Tan H W,Zhu Q Z,Wang W Z,Yang L.Development and progress of sugarcane farming technologies in Guangxi,China[J].Journal of Southern Agriculture,2014,45(10):1770-1775(in Chinese)
[4]Waclawovsky A J,Sato P M,Lembke C G,Moore P H,Souza G M.Sugarcane for bioenergy production:an assessment of yield and regulation of sucrose content.Plant Biotechnology Journal,2010,8:263-276
[5]Tew T L.World sugarcane variety census-year 2000[J].Sugar Cane International,2003,2(March/April):12-18
[6]张木清,王华忠,白晨.糖料作物遗传改良与高效育种[M].北京:中国农业出版社,2006:1-252Zhang M Q,Wang H Z,Bai C.Genetic Improvement and Highly Efficient Breeding for Sugar Crop[M].Beijing:China Agricultural Press,2006:1-252(in Chinese)
[7]李杨瑞.现代甘蔗学[M].北京:中国农业出版社,2010:103-116.Li Y R.Modern Sugarcane Science[M].Beijing:China Agriculture Press,2010:103-106(in Chinese)
[8]Nair N V.Sugarcane varietal development programmes in India:An overview[J].Sugar Tech,2011,13(4):275-280
[9]王丽萍,马丽,夏红明,陆鑫,蔡青,范源洪,陈辉,刘新龙.甘蔗细茎野生种(S spontaneum)在杂交育种中的利用[J].中国糖料,2006,2(1):1-4Wang L P,Ma L,Xia H M,Lu X,Cai Q,Fan Y H,Chen H,Liu X L.Application of S spontaneumin sugarcane cross-breeding.Sugar Crops of China,2006,2(1):1-4(in Chinese)
[10]桃联安,经艳芬,董立华,安汝东,杨李和,周清明,段慧芬,朱建荣.云南甘蔗细茎野生种82-114测交后代主要性状遗传分析[J].植物遗传资源学报,2011,12(3):419-424Tao L A,Jing Y F,Dong L H,An R D,Yang L H,Zhou Q M,Duan H F,Zhu J R.Genetic analysis of main traits in descendants of crossing with Saccharum spontaneum 82-114in Yunnan.Journal of Plant Genetic Resources,2011,12(3):419-424(in Chinese)
[11]陈建文,Nathalie Piperidis,李奇伟,陈勇生,符成,Phillip Jackson,George Piperidis,邓海华.用基因组原位杂交方法分析甘蔗-斑茅杂种及回交后代的染色体组成[J].分子植物育种,2010,2(1):293-296Chen J W,Nathalie Piperidis,Li Q W,Chen Y S,Fu C,Phillip Jackson,George Piperidis,Deng H H.Chromosome pattern of the hybrids and their backcross progenies derived from sugarcane and Erianthus arundinaceus identified by in situ genomic hybridization.Molecular Plant Breeding,2010,2(1):293-296(in Chinese)
[12]Piperidis N,Chen J W,Deng H H,Wang L P,Jackson P,Pepiridis G.GISH characterization of Erianthus arundinaceus chromosomes in three generations of sugarcane intergeneric hybrids[J].Genome,2010,53:331-336
[13]刘少谋,符成,陈勇生.近十年海南甘蔗育种场斑茅后代回交利用研究[J].甘蔗糖业,2007(4):1-6Liu S M,Fu C,Chen Y S.Utilization of Erianthus arundinaceus progeny through backcross over the last decade at Hainan sugarcane breeding station[J].Sugarcane and Cane Sugar,2007(4):1-6(in Chinese)
[14]王丽萍,蔡青,范源洪,陆鑫,Karen Aiken,马丽,刘新龙,夏红明.甘蔗(Saccharum)与斑茅(Erianthus arundinaceus)远缘杂交利用研究[J].西南农业学报,2007,20(4):721-726Wang L P,Cai Q,Fan Y H,Lu X,Karen Aiken,Ma L,Liu X L,Xia H M.Study on the distant hybrid utilization between Saccharum and Erianthus arundinanceus[J].Southwest J Journal Agric Sci,2007,20(4):721-726(in Chinese)
[15]刘昔辉,方锋学,高轶静,张荣华,宋焕忠,杨荣仲,方位宽,段维兴,罗霆,张革民,李杨瑞.斑茅割手密杂种后代真实性鉴定及遗传分析[J].作物学报,2012,38(5):914-920Liu X H,Fang F X,Gao Y J,Zhang R H,Song H Z,Yang R Z,Fang W K,Duan W X,Luo T,Zhang G M,Li Y R.Identification and genetic analysis of hybrid from cross between Erianthus arundinacius(Retz)Jesws and Saccharum spontaneum L[J].Acta Agronomica Sinica,2012,38(05):914-920(in Chinese)
[16]高轶静,方锋学,刘昔辉,张荣华,宋焕忠,杨荣仲,罗霆,段维兴,游建华,张革民.甘蔗与斑茅割手密复合体杂交后代的分子鉴定[J].植物遗传资源学报,2012,13(5):912-916Gao Y J,Fang F X,Liu X H,Zhang R H,Song H Z,Yang R Z,Luo T,Duan W X,You J H,Zhang G M.Identification of progeny from crosses between sugarcane(Saccharum spp.)and intergeneric hybrid complex(Erianthus arundinaceus×Saccharum spontaneum)with molecular markers[J].Journal of Plant Genetic Resources,2012,13(5):912-916(in Chinese)
[17]张志良.植物生理学实验手册[M].北京:高等教育出版社,1998.33-38Zhang Z L.The Laboratory Methods of Plant Physiology[M].Beijing:Higher Education Press,1998:33-38(in Chinese)
[18]Jiang N,Di Y T,Zhao G C,Xu K Z,Wu Z H,Zhang Z A,Ling F L.Biomasses in different organs of rice cultivars developed during recent forty-seven years in Jilin Province,China[J].Rice Science,2010,17(3):206-212
[19]Tollenaar M.Genetic improvement in grain yield of commercial maize hybrids grown in Ontario from 1959to 1988[J].Crop Science,1989,29:1365-1371
[20]胡昌浩,董树亭,王空军,孙庆泉.我国不同年代玉米品种生育特性演进规律研究:Ⅱ.物质生产特性的演进[J].玉米科学,1998,6(3):49-53Hu C H,Dong S T,Wang K J,Sun Q Q.Study of growth and development evolution characteristics in maize varieties released in different years:Ⅱ.Evolution of dry matter production[J].Journal of Maize Sciences,1998,6(3):49-53(in Chinese)
[21]Brancourt H M,Doussinault G,Lecomte C,Berard P,Le B B,Trottet M.Genetic improvement of agronomic traits of winter wheat cultivars released in France from 1946to 1992[J].Crop Science,2003,43:37-45
[22]Shearman V J,Sylvester-Bradley R,Scott R K,Foulkes M J.Physiological processes associated with wheat yield progress in the UK[J].Crop Science,2005,45:175-185
[23]Cober E R,Morrison M J,Ma B,Butler G.Genetic improvement rates of short-season soybean increase with plant population[J].Crop Science,2005,45:1029-1034
[24]周鸿凯,刘桂富,蔡华斌,王尚钦,郭荣发.甘蔗生物量育种的ADGE遗传分析[J].热带亚热带植物学报,2005,13(3):259-263Zhou H K,Liu G F,Cai H B,Wang S Q,Guo R F.Genetic analysis of biomass in sugarcane using ADGE model[J].Journal of Tropical and Subtropical Botany,2005,13(3):259-263(in Chinese)
[25]Legendre B L,Burner D M.Biomass production of sugarcane cultivars and early-generation hybrids[J].Biomass Bioenergy,1995,8(2):55-61
[26]Russell R S.Plant Root System:Their Function and Interaction with the Soil[M].London:McGraw-Hill Book Co,1997:12-15
[27]De dorlodot S,Forster B,Pages L,Price A,Tuberosa R,Draye X.Root system architecture:Opportunities and constraints for genetic improvement of crops[J].Trends in Plant Science,2007,12(10):470-481
[28]程式华,曹立勇,陈深广,朱德峰,王熹,闵绍楷,翟虎渠.后期功能型超级杂交稻的概念及生物学意义[J].中国水稻科学,2005,19(3):280-284Cheng S H,Cao L Y,Chen S G,Zhu D F,Wang X,Min S K,Zhai H Q.Conception of late-stage vigor super hybrid rice and its biological significance l[J].Chinese Journal of Rice Science,2005,19(3):280-284(in Chinese)
[29]Fitter A.Characteristics and functions of root systems[M].In:Waisel Y,Eshel A,Kafkafi U,Plant Roots,the Hidden Half.New York:Marcel Dekker Inc,2002:15-32